Extended-Gate Metal Oxide Semiconductor Field Effect Transistor-Based Biosensor for Detection of Deoxynivalenol

Kwon, Insu,Lee, Hee-Ho,Choi, Jinhyeon,Shin, Jang-Kyoo,Seo, Sang-Ho,Choi, Sung-Wook,Chun, Hyang Sook Japan Society of Applied Physics 2011 Japanese journal of applied physics Vol.50 No.6

Protective effects of endurance exercise on skeletal muscle remodeling against doxorubicininduced myotoxicity in mice

( Insu Kwon ) 한국운동영양학회 2020 Physical Activity and Nutrition (Phys Act Nutr) Vol.24 No.2

[Purpose] Doxorubicin (DOX) is a potent anti-cancer drug that appears to have severe myotoxicity due to accumulation. The skeletal muscle has a regeneration capacity through satellite cell activation when exposed to extracellular stimulus or damage. Endurance exercise (EXE) is a therapeutic strategy that improves pathological features and contributes to muscle homeostasis. Thus, this study investigated the effect of EXE training in mitigating chronic DOX-induced myotoxicity. [Methods] Male C57BL/6J mice were housed and allowed to acclimatize with free access to food and water. All the mice were randomly divided into four groups: sedentary control (CON, n=9), exercise training (EXE, n=9), doxorubicin treatment (DOX, n=9), doxorubicin treatment and exercise training (DOX+EXE, n=9) groups. The animals were intraperitoneally injected with 5 mg/kg/week of DOX treatment for 4 weeks, and EXE training was initiated for treadmill adaptation for 1 week and then performed for 4 weeks. Both sides of the soleus (SOL) muscle tissues were dissected and weighed after 24 hours of the last training sessions. [Results] DOX chemotherapy induced an abnormal myofiber’s phenotype and transition of myosin heavy chain (MHC) isoforms. The paired box 7 (PAX7) and myoblast determination protein 1 (MYOD) protein levels were triggered by DOX, while no alterations were shown for the myogenin (MYOG). DOX remarkably impaired the a-actinin (ACTN) protein, but the EXE training seems to repair it. DOX-induced myotoxicity stimulated the expression of the forkhead box O3 (FOXO3a) protein, which was accurately controlled and adjusted by the EXE training. However, the FOXO3a-mediated downstream markers were not associated with DOX and EXE. [Conclusion] EXE postconditioning provides protective effects against chronic DOX-induced myotoxicity, and should be recommended to alleviate cancer chemotherapy- induced late-onset myotoxicity.

Angiotensin-converting enzyme gene insertion/deletion polymorphism is not associated with BMI in Korean adults

( Insu Kwon ) 한국운동영양학회 2020 Physical Activity and Nutrition (Phys Act Nutr) Vol.24 No.1

[Purpose] Recent studies have demonstrated a probable association between ACE I/D polymorphism and obesity. Thus, this study aimed to investigate whether ACE I/D polymorphism influenced the susceptibly of developing obesity in Korean adults. [Methods] A total of 353 healthy Korean adults aged between 30 and 82 years were recruited, including 157 males and 196 females. Among the participants, 103 (29.2 %) were classified as normal (BMI < 23 kg/m²), 117 (33.1 %) as overweight (23 kg/m² ≤ BMI < 25 kg/ m²), and 133 (37.7 %) as obese (BMI ≥ 25 kg/m²). ACE polymorphism (rs1799752) analysis was performed using the MGB TaqMan® SNP Genotyping assay with 3 types of primers and 2 types of probes. The distributions of the ACE genotypes and allele frequencies were analyzed among the three groups using the Hardy- Weinberg equilibrium, chi-square tests, and multiple regression analysis. [Results] The distribution of the ACE genotypes were as follows: normal [II: n=38 (36.9 %), ID: n=46 (36.8 %), DD: n=19 (18.4 %)], overweight [II: n=43 (36.8 %), ID: n=55 (47.0 %), DD: n=19 (16.2 %)], and obese [II: n=41 (30.8 %), ID: n=76 (57.0 %), DD: n=16 (12.0 %)]. Unexpectedly, the I allele, rather than the D allele, was common in the obese group. [Conclusion] ACE I/D polymorphism is not associated with BMI in Korean adults. Thus, it is unlikely to be a powerful candidate gene for obesity in Korean adults.

Effects of long-term resistance exercise training on autophagy in rat skeletal muscle of chloroquine-induced sporadic inclusion body myositis

( Insu Kwon ),( Youngil Lee ),( Ludmila M. Cosio Lima ),( Joon Yong Cho ),( Dong Chul Yeom ) 한국운동영양학회 2015 Physical Activity and Nutrition (Phys Act Nutr) Vol.19 No.3

[Purpose] We examined whether resistance exercise training restores impaired autophagy functions caused by Chloroquine (CQ)-induced Sporadic Inclusion Body Myositis (sIBM) in rat skeletal muscle. [Methods] Male wistar rats were randomly assigned into three groups: Sham (n = 6), CQ (n = 6), and CQ + Exercise (CE, n = 6). To create a rat model of sIBM, rats in the CQ and CE group were intraperitoneally injected with CQ 5 days a week for 16 weeks. Rats in the CE group performed resistance exercise training 3 times a week for 8 weeks in conjunction with CQ starting from week 9 to week 16. During the training period, maximal carrying load, body weight, muscle weight, and relative muscle weight were measured. Autophagy responses were examined by measuring specific markers. [Results] While maximal carrying capacity for resistance exercise training was dramatically increased in the CE group, no significant changes occurred in the skeletal muscle weight as well as in the relative muscle weight of CE compared to the other groups. CQ treatment caused significant increases in the levels of Beclin-1 and p62, and decreases in the levels of LAMP-2 proteins. Interestingly, no significant differences in the LC3-II/I ratio or the LC3-II protein levels were observed. Although CQ-treatment groups suppressed the levels of the potent autophagy inducer, BNIP3, p62 levels were decreased in only the CE group. [Conclusion] Our findings demonstrate that sIBM induced by CQ treatment results in muscle degeneration via impaired autophagy and that resistance exercise training improves movable loading activity. Finally, regular exercise training may provide protection against sIBM by enhancing the autophagy flux through p62 protein.

다양한 기록 채널에 적응 가능한 PRML 방법 및 구현

권인수(Insu Kwon),권석범(Sokbom Kwon),이재진(Jaejin Lee) 한국통신학회 2003 한국통신학회 학술대회논문집 Vol.2003 No.7

인구통계적 응집성이 기업성과에 미치는 영향에서 HR 제도의 역할 탐구 : 순환근무와 탄력근무를 중심으로

권인수(Insu Kwon),이하은(Ha-eun Lee),김상준(Sang-Joon Kim) 강원대학교 경영경제연구소 2021 Asia-Pacific Journal of Business Vol.12 No.4

Purpose - The purpose of this study is to specify the relationship between demographic cohesion and firm performance by examining the roles of HR practices, such as job rotation and flexible work arrangement. Design/methodology/approach - This study samples 1,093 firms in Korea and collects their data between 2007 and 2017 from Workplace Panel Survey, a database from Korea Labor Institute. The demographic cohesion is measured using the Herphindal-Hershman index and the firm performance is measured with net incomes. This study employs a fixed-effects model for the estimation of firm performance with respect to demographic cohesion, job rotation, and flexible work arrangement. Findings - There is a positive relationship between demographic cohesion and net incomes. And the relationship is positively moderated by job rotation. However, flexible work arrangement shows a mixed moderation. Research implications or Originality - Differentiated from the studies on demographic diversity, this study shows that demographic cohesion has a mixed impact on firm performance. While demographic cohesion can improve firm performance through trust building, in-group favoritism, and collective identity it entails internal conflicts. However the link between demographic cohesion and firm performance is moderated by job rotation and flexible work arrangement. While there is a positive moderation of job rotation, there is a negative moderation of flexible work arrangement.

Effect of Silk Fibroin Biomaterial Coating on Cell Viability and Intestinal Adhesion of Probiotic Bacteria

( Gicheol Kwon ),( Bohye Heo ),( Mi Jin Kwon ),( Insu Kim ),( Jaeryang Chu ),( Byung-yong Kim ),( Byoung-kook Kim ),( Sung Sun Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.4

Probiotics can be processed into a powder, tablet, or capsule form for easy intake. They are exposed to frequent stresses not only during complex processing steps, but also in the human body after intake. For this reason, various coating agents that promote probiotic bacterial stability in the intestinal environment have been developed. Silk fibroin (SF) is a material used in a variety of fields from drug delivery systems to enzyme immobilization and has potential as a coating agent for probiotics. In this study, we investigated this potential by coating probiotic strains with 0.1% or 1% water-soluble calcium (WSC), 1% SF, and 10% trehalose. Under simulated gastrointestinal conditions, cell viability, cell surface hydrophobicity, and cell adhesion to intestinal epithelial cells were then measured. The survival ratio after freeze-drying was highest upon addition of 0.1% WSC. The probiotic bacteria coated with SF showed improved survival by more than 10.0% under simulated gastric conditions and 4.8% under simulated intestinal conditions. Moreover, the cell adhesion to intestinal epithelial cells was elevated by 1.0-36.0%. Our results indicate that SF has positive effects on enhancing the survival and adhesion capacity of bacterial strains under environmental stresses, thus demonstrating its potential as a suitable coating agent to stabilize probiotics throughout processing, packaging, storage and consumption.

리더-구성원의 교환관계가 심리적 주인의식에 미치는 영향

권인수(Kwon Insu),서문교(Seo Moonkyo),고병기(Koh Byoungki) 한국인적자원개발학회 2017 인적자원개발연구 Vol.20 No.4

소명의 사후적 형성

권인수(Insu Kwon),김상준(Sang-Joon Kim) 연세대학교 경영연구소 2017 연세경영연구 Vol.54 No.1

본 연구는 소명의식의 개념을 재조명하여, 소명의식이 시간의 흐름에 따라 변할 수 있음을 주장한다. 지금까지의 소명의식에 대한 연구는 선험적으로 형성되어 시간의 흐름에 따라 변화하지 않을 것이라는 가정에 기초하고 있으나, 본 연구는 조직 내에서의 직급이 시간과 밀접하게 연동되어 있는 조직 구성요소임에 착안하여 직급에 따라 소명의식이 달라짐을 보여 주고자 하였다. 직급은 단순히 조직의 직제 상에 표기된 지위뿐만 아니라 경력개발 측면에서의 사회적 규범이라는 특성을 지닌다. 여기서 사회적 규범이란 특정한 직급에 도달하기 위해서 시간이 필요함을 인식하고, 어느 정도의 시간을 투자해야 특정한 직급으로 승진할 수 있는지를 의미한다. 본 연구는 어떤 개인이 사회적 규범보다 이른 승진인지 아니면 늦은 승진을 했는 지를 측정하여 실제 경험하는 직급 외에 인지적으로 위치한 직급 역시 소명의식 형성에 영향을 줄 것이라는 주장을 검토하였다. 분석 대상의 경력개발단계 및 소속 조직특성의 일관성을 위해서 은행 19개의 211명의 은행원으로부터 수집된 자료를 바탕으로 직급과 소명의식 간의 관계를 분석하였다. 그 결과, 직급에 따라 소명의식이 차이가 있음을 발견하였고, 또 코호트 분석을 통해 간접적이지만 소명의식은 직급 코호트에 가변적인 모습을 보이고 있음을 발견하였다. 이는 곧, 소명의식은 사후적으로 형성될 수 있음을 보여주는 것으로 해석된다. 본 연구는 소명의식의 사후적 형성에 대한 탐색적 연구의 성격을 띄며, 직업에 대한 의미부여나 센스메이킹에 대한 연구를 소명의식 관점에서 시도하였다는 점에서 그 이론적, 실무적 시사점을 찾을 수 있다. This study revisits the concept of calling, assumed as fixed or ex ante, by examining the possibilities that one’s sense around calling can be changed over time. In particular, given that ranks in organization can represent one’s career achievement as well as his/her work experiences, we argue that the sense of a calling can be dependent on the level of ranks. Drawn from social construction theory, we attempt to reconceptualize rank in organization with the level of rank and the norm-adaptive rank. First, the level of rank can be understood as what an employee has achieved in his/her organization. This sense of achievement with the level of rank can make his/her job more meaningful, reinforcing the sense of a calling. On the other hand, we posit that a particular rank requires time duration, which is either formalized in the promotion ladder or acknowledged as a social norm based on one’s age or work duration. If one gets promoted fast or slow, he/she will violate the social norm. This deviation will influence how employees perceive a calling. As such, whether an employee can adapt to the social norm or not can be another factor to change the sense of a calling. To examine this idea, we surveyed 211 employees in 19 bank and financial companies in Korea. And we investigated how the level of rank and the norm-adaptive rank can influence the sense of a calling. We found two things. First, employees at the higher rank tend to perceive a stronger sense of calling. Second, fast promotion relative to the social norm is likely to collapse a sense of a calling while slow promotion tends to reinforce the sense of a calling. These findings reveal that sense of a calling is neither static nor ex ante, but dynamic and ex post. Our study is the first attempt in Korea to illuminate the non-static character of calling. We discuss how we can understand this character of calling and provide some theoretical and practical implications.

직무스트레스의 선행요인과 상사-부하간 교환관계(LMX) 질의 조절효과

권인수(Insu Kwon),백승무(Seungmoo Baik) 연세대학교 경영연구소 2011 연세경영연구 Vol.48 No.1

본 연구는 직무스트레스의 선행요인과 조절요인에 관한 연구로서 첫째, 군 조직구성원을 대상으로 직무스트레스의 선행요인의 영향력을 분석하였으며, 둘째 이들 선행요인과 직무스트레스의 관계를 상사-부하간 교환관계(LMX) 질이 조절하는지를 분석하였다. 연구결과 신체적 스트레스에 대해서는 역할모호성, 역할과부하, 경력관리 그리고 의사소통이, 심리적 스트레스에 대해서는 역할갈등, 역할모호성, 역할과부하, 분배공정성 그리고 경력관리가 유의한 영향을 미치는 것으로 나타났다. 그리고 역할특성, 직무특성 그리고 조직특성 등의 선행요인들과 직무스트레스의 관계에서 LMX 질의 조절효과가 부분적으로 확인되었다. This study investigates the antecedents of the job stress and the moderating effect of Leader-Member Exchange quality between the antecedents and two forms of job stress, physical and psychological stress. Results suggest role ambiguity, role overload, career development, and communication are significantly associated with physical stress. And role conflict, role ambiguity, role overload, organizational justice, and career development have significant effect on psychological stress. Also, LMX quality had a partial moderating effect between the antecedents and job stress.