DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

정안침요법(整顔鍼療法)을 응용하여 호전된 후천성 사경환자 치험 2례

박서영,김재수,홍의재,이윤경,이봉효,임성철,정태영,이경민,Park, Seo-Young,Kim, Jae-Su,Hong, Eui-Jae,Lee, Yoon-Kyoung,Lee, Bong-Hyo,Lim, Seong-Chul,Jung, Tae-Young,Lee, Kyung-Min 대한침구의학회 2008 대한침구의학회지 Vol.25 No.4

Objectives : This report intended to estimate effects of Jung-an acupuncture on the acquired torticollis caused by muscle contraction. Methods : The patients were treated by Jung-an acupuncture, herb medication and Physical treatment daily. The improvement of the patients was judged by Toronto Western Spasmodic Torticollis Rating Scale(TWSTRS), Tsui's score and Visual Analogue Scale(VAS). Results : After 7 days of treatment, the 2 patients' neck pain and cervical movement were improved. Also, TWSTRS score, Tsui's score and VAS were all reduced. Conclusions : As the Jung-an acupuncture's theological base is organized with meridian theory and myofacial release therapy, We considered the Jung-an acupuncture is effective not only for the skin disease but also for the muscle problem such as torticollis.

Environmental Microbiology / Microbial Diversity : Calcium Carbonate Precipitation by Bacillus and Sporosarcina Strains Isolated from Concrete and Analysis of the Bacterial Community of Concrete(s)

( Hyun Jung Kim ),( Hyo Jung Eom ),( Chulwoo Park ),( Jaejoon Jung ),( Bora Shin ),( Wook Kim ),( Namhyun Chung ),( In Geol Choi ),( Woojun Park ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.3

Microbially induced calcium carbonate precipitation (CCP) is a long-standing but re-emerging environmental engineering process for production of self-healing concrete, bioremediation, and long-term storage of CO2. CCP-capable bacteria, two Bacillus strains (JH3 and JH7) and one Sporosarcina strain (HYO08), were isolated from two samples of concrete and characterized phylogenetically. Calcium carbonate crystals precipitated by the three strains were morphologically distinct according to field emission scanning electron microscopy. Energy dispersive X-ray spectrometry mapping confirmed biomineralization via extracellular calcium carbonate production. The three strains differed in their physiological characteristics: growth at alkali pH and high NaCl concentrations, and urease activity. Sporosarcina sp. HYO08 and Bacillus sp. JH7 were more alkali- and halotolerant, respectively. Analysis of the community from the same concrete samples using barcoded pyrosequencing revealed that the relative abundance of Bacillus and Sporosarcina species was low, which indicated low culturability of other dominant bacteria. This study suggests that calcium carbonate crystals with different properties can be produced by various CCP-capable strains, and other novel isolates await discovery.

치매방지 작업기구 사용이 항산화효소 활성에 미치는 영향

조경혜,박정은,윤지윤,문세보,박효영,정인옥,이정원 서울여자대학교 자연과학연구소 2001 자연과학연구논문집 Vol.13 No.-

We developed an occupational therapy tool to maintain and improve the cognitive function for the elderly who have senile dementia. 86 elderly people took a mininal mental state examination (MMSE) for the therapy to perform the occupational therapy, and divided into three groups based on avarage ages and MMSE values. The occupational therapy was treated three times per week for 10 weeks. The results showed that there were significant differences in the performing scores before and after the treatment ; the scores for the occupational therapy were increased significantly (p<0.001) and the performing time was shortened from 6-week throughout 10-week regimen. This study was to examine the effect on the activity of antioxidant enzymes by occupational therapy. The activities of antioxidant enzymes such as catalase, Glutathion-6-phosphate dehydrogenase (G6PDH), and superoxide dismutase (SOD) were increased to the normal standard by the occupational therapy for the elderly having dementia. The recovery of activities of antioxidant enzymes was obvious in severe demented elderly compared to those in normal or mild demented elderly. These results suggest that the occupational therapy had an beneficial effect on the protection against the oxidative stress especially for severe demented elderly, and the occupational tool need to be modified so as to improve the protection against the oxidative stress for normal and/or mild demented elderly.

북한산국립공원 북한산성-인수봉 지역의 산림군집구조

추갑철 ( Gab Cheul Choo ),엄태원 ( Tae Won Um ),김갑태 ( Gab Tae Kim ),박삼봉 ( Sam Bong Park ),안효현 ( Hyo Hyeon An ),김남호 ( Nam Ho Kim ),김희정 ( Hee Jung Kim ) 한국환경생태학회 2008 한국환경생태학회지 Vol.22 No.2

북한산 국립공원지역의 식생구조를 파악하고자, 북한산성에서 인수봉에 이르는 지역에 16개의 방형구(2500㎡)를 설정하여 식생을 조사하였다. 식물군집을 분류한 결과 16개 조사구는 신갈나무 혼효림 군집, 소나무-신갈나무 군집, 신갈나무 우점군집의 3개 군집으로 분류되었다. 북한산 국립공원내 북한산성에서 인수봉에 이르는 지역의 우점수종은 신갈나무로 나타났다. 수종간의 상관관계는 신갈나무와 진달래, 신갈나무와 개옻나무, 진달래와 철쭉, 노린재나무와 철쭉, 당단풍과 함박꽃나무 등의 수종들 간에는 높은 정의상관이 인정 되었고, 물푸레나무와 진달래, 노간주나무와 당단풍, 쪽동백나무와 당단풍 등의 수종들간에 부의 상관이 비교적 높은 편이다. 조사지의 군집별 종 다양성도는 1.23 6~1.319의 범위로 다른 국립공원들의 능선부 식생에 비하여 비교적 높게 나타났다. To investigate the vegetation structure of the region from Bukhansanseong to Insubong in Bukhansan National park, 16 plost(2500㎡) set up with random sampling method were surveyed. Three groups Quercus mongolica -Mixed Broad Ieaved community, Pinus densiflora -Quercus mongolica community, Quercus mongolica community was classified (or communities were classified) by cluster analysis. Quercus mongolica were found as a major woody plant species in Bukhansan National park region. High positive correlation were proved between Quercus mongolica and Rhododendron; Quercus mongolica and Rhus tricocarpa; Rhododendron mucronulatum and Rhododendron schlippenbachi; Symplocos chinensis and Rhododendron schlippenbachii; Acer pseudosieboldianum and Magnolia sieboldii, and relatively high negative correlation was proved between Fraxinus rhynchophylla and Rhododendron mucronulatum; Juniperus rigida and Acer pseudosieboldianum; Styrax obassia and Acer pseudosieboldianum. Species diversity(H`) of investigated groups were ranged from 1.236~1.319 and it was relatively high compared to those of the ridge area of other national parks.

한계계단 응답특성을 이용한 영구자석형 동기전동기 속도제어

박승엽,전인효,최중경 창원대학교 공작기계기술연구센터 1999 연구업적집 Vol.1 No.1

본 논문에서는 한계계단 응답특성을 이용하여 영구자석형 동기전동기의 속도 제어계에 대한 새로운 자동 동조 PI(Proportional Integral)제어기를 설계하였다. 과도응답 기간동안 소위 한계입력이라고 하는 계단입력을 인가하여 PI 제어기의 자동동조를 위한 정보를 얻기 위한 기법을 제안한다. 시스템 파라메터 추정과 동시에 속도제어가 온라인으로 이루어지는데, 이것은 시스탬의 불확실성에도 불구하고 한계입력에 의해서 얻어진 시스템 파라메터 정보가 연속적으로 PI 제어기에 적용될 수 있다는 것이다. 제안된 자동동조 알고리즘은 단조증가 계단응답 특성을 갖는 영구 자석형 동기전동기의 속도제어에 대한 시뮬레이션과 실험결과를 통하여 그 유효함을 확인하였다. In this paper, a new auto-tuning PI controller for the speed servo system of a PMSM is designed by using limited step response characteristics. The method is proposed that gets information about auto-tuning of PI regulator by the injection fo step input, called limited input, during a transient response time of control. System parameter estimation and speed control could be continuously executed. This means that in despite of system uncertainty the system information obtained by limited input can be continuously applied to the PI regulator. We demonstrate the effectiveness of the proposed auto-tuning algorithm through simulation and experiment result of the speed control for a PMSM having monotone increasing step response.

Mild Cognitive Impairment 노인군의 점화효과 특성

박재우,김도관,고선규,고효정,김성환,김재경,서민영,이인수,김지혜 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.3

연구목적 : 이 연구에서는 K-MMSE에서 25점 이하를 받은 50대 이상 노인들을 MCI(Mild Cognitive Impairment) 군으로 보고 이들의 인지 기능 특성을 점화 효과를 중심으로 살펴보고자 하였다. 먼저, 점화 효과가 MCI 노인군에서 있어서도 유지되고 있는지를 알아보고, 인지 기능의 손상 정도에 따라 점화 효과가 유의미하게 변화하는 지를 살펴보고자 하였다. 방 법 : 본 연구에서는 K-NAME 점수 25점 이하의 노인 96명으 대상으로 ADAS-Cog와 일종의 간접 기억 검사 도구인 지각적 파악 과제를 제작하여 점화 효과를 측정하여 인지 기능의 퇴행이 시작된 고연령군이 나타내는 암묵 기억 능력 특성을 알아보았다. 점화효과는 컴퓨터 상에 사전 노출된 자극과 그렇지 않은 자극을 순간 노출시켜 미리 노출된 자극에 대해 반응 시간이 줄어들고 정확 반응이 증가하는 정도로써 측정하였다. 이 때 지능이나 연령, 학력 수준과 같은 변인이 미치는 영향을 통제하기 위해 한국판 웩슬러 지능 검사의 <어휘>, <토막> 소검사를 실시하였고, 인구통계학적 변인에 관한 면접을 실시하였다. 통계적 분석 과정에서는 먼저, 연구에서 측정된 점화효과의 특성을 알아보기 점화 효과 측정치의 기술 통계치를 구하였으며 다른 측정 변인들과의 상관 분석을 실시하였다. 마지막으로 측정된 점화효과가 인지 기능의 퇴행 수준에 비례하는 지의 여부를 파악하기 위해 ADAS-Cog 및 나이, 교육 연한 및 지능 수준을 독립변인으로 회귀분석을 실시하였다. 결 과 : 먼저 기술 통계 분석 결과에 의하면 본 연구에 참여한 피보험자들의 경우 점화효과에 의해 미리 노출된 자극을 평균 3.6개 더 정확히 파악하였으며 평균 59ms 더 빠르게 반응한 것으로 나타나 이들 집단에서도 점화효과가 유지되고 있음을 알 수 있었다. 다음으로 변인들 간의 상관 분석 결과에 따르면, 정확 파악 반응 점수에 따른 점화효과는 전반적인 인지 기능을 평가하는 K-MMSE 및 ADAS-Cog와 정적인 상관이 있는 것으로 나타났으며 반응 속도로 측정한 점화효과의 경우에는 나이와는 정적인 상관이, KWIS의 <어휘> 소검사 점수와는 부적인 상관이 있는 것으로 나타났다. 또한, 본 연구에서 사용된 점화 효과 측정치를 종속 변인으로 ADAS-Cog와 KWIS의 어휘 및 코막 소검사로 측정한 지능 수준, 나이와 교육 연한과 같은 인구 통계학적 변인을 독립 변인으로 회귀 분석을 실시한 결과에 따르면 정화간응 점수로 측정한 점화 효과의 경우 상관 분석 결과에서와 같이 ADAS-Cog 변인만이 유의미한 효과를 갖는 것으로 나타났으며 반응 속도로 측정한 점화 효과의 경우 나이의 효과만 유의미한 것으로 나타났다. 결 론 : 이러한 결과는 경미한 인지 기능의 손상이 진행되고 있는 고연령군의 경우에는 외현 기억 능력은 손상이 시작되지만 점화효과로 측정된 암묵 기억이 전반적으로는 유지되다가 인지 기능의 퇴행이 진행됨에 따라 노출된 자극을 더 정확히 파악하는 능력이 저하되고 점화 효과에 의해 인출 속도가 빨라지는 현상이 연령이 증가함에 따라 함께 증가하는 것으로 나타남을 말해 준다. Objectives : In this study, the cognitive characteristics, especially priming effect, of above 50 years old age group who had less than 25 points in K-MMSE were investigated. Methods : In this study, an implicit memory test measuring priming effect was made and administered ti old age people who had less than 25 points in k-MMSe in screening session. To control the effect of age, educational level and intelligence, demograohic variables were measured, and the subjects of KWIS, vocabulary and block design were administered. Descriptive statistical analysis of the two priming measures and correlational analysis between variables were done. To test the effect of cognitive functioning on priming effect multiple regression analysis was done. Results : Correlational analysis revealed priming score obtained from correct identification response was positively correlated with K-MMSE and ADAS-Cog. And priming score obtained from mean reaction time was positively correlated with age and negatively correlated with vocabulary substest of KWIS. The regression analysis results indicated from correct identification response, whereas age has significant effect on priming score obtained from mean reaction time. Conclusions : The results revealed that as cognitive deterioration progresses priming effect which identify primed stimulus correctly diminish, and as one grow older within age 50 to 70, priming effect which identifies primed stimulus quickly increase.

체중감량 프로그램에 따른 체력의 변화에 대한 연구 : by Obese Female College Students in Seoul

박효정,장도석,장은재 동덕여자대학교 2003 생활과학연구 Vol.8 No.-

The purpose of this study was to investigate the effect of the aerobic exercise with calorie restricted diet on the change of the body composition, health-related fitness and performance-related fitness during 4 weeks. Twelve obese female college students ages from 20 to 24 who participated in this program. Variables were body composition; Fat mass, Fat free mass. Fat percentage, PIBW(Percentage of ideal Body Weight), BMI(Body Mass Index), health-related fitness and performance-related fitness. The results were as following; First. Diet was found to have a significant decrease of 266㎉ in the change of energy intake and exercise was found to have a significant consume of 309.71㎉ in the change of energy expenditure. Second, Weight, BMI, PIBW, percent body fat, fat mass were found to have a significant decrease of 5.02%, 5.01%, 5.25%, 2.58%, and 2.61% in the change of the body composition, respectively. (P>0.05) FFM was also decreased but it was not significantly. Third, Grip strength and Sit-ups were found to have a significant increase of 2.69% and 2.78% in the change of health-related fitness. (P>0.05) Back strength, Sitting trunk flexion and VO2max were found to have increase of 1.47%, 0.35% and 0.75% in the change of health-related fitness. Forth, Whole body reaction time was found to have a significant decrease of 2.62% in the change of performance-related fitness. Vertical jump and Closed eyes-foot balance were found to have a increase of 1.96% and 1.79% in the change of performance-related fitness. As the results, the weight control program demonstrated to have a positive effect in the improvement of the body composition, health-related fitness and performance-related fitness in obese female.

중학교 수학에서의 대수개�牟�대한 이해와 적용에 관한 연구 : 중학교 3학년을 중심으로 For the third grade of middle school

朴順喆,朴孝精 조선대학교 사범대학 부설 교과교육연구소 1999 敎科敎育硏究 Vol.2 No.1

국가 및 공공기관 소속 전문박물관의 참여전시 연구 : 대법원 법원전시관 중심으로

박효정 국립중앙박물관 2019 박물관 교육 Vol.3 No.-

박물관은 전시주제에 따라 종합박물관과 전문박물관으로 구분된다. 과학의 발전은 소장품의 전문적 분류를 야기했고 이는 전문화된 박물관 발생으로 이어졌다. 전문박물관은 국가 및 공공기관에서 국가의 문화발전과 경제발전을 위하고 그 기관의 홍보와 이미지 향상 그리고 기관의 서비스 보존, 계승, 발전을 알리기 위해 설립되었다. 그러나 전문박물관의 양적 성장에 비해 운영과 활성화 측면에서 국립박물관으로 등록했음에도 불구하고 전문 인력의 부족으로 더딘 성장을 보였다. 본 연구는 국가 및 공공 기관 소속 박물관의 더딘 성장의 이유와 내부적 한계점 및 문제점, 극복 사례를 대법원 소속 법원전시관을 통해 살펴보았다. 대법원 소속 법원박물관은 전문인력 부족, 소장품 결여와 기관 내 홍보관이나 자료관으로 인식되는 이미지가 있었다. 이런 문제점을 해결하기 위해 참여모델의 전문가인 니나 사이먼의 ‘협력 프로젝트-공동개발형’ 모델을 전시에 시도하였다. 그 결과 박물관의 전시 방향이 관람자 중심으로 바뀌었고 내부적으로도 관람자 중심의 박물관 운영을 이해하게 되었다. The establishment of museums with a specialty under governmental or public agencies aims to realize public relations that manifest the preservation and succession of those agencies. Since the 2000s, however, their operation and vitalization have developed at a relatively slower pace compared to their rapid increase in numbers. The major problems of museums with a specialty under the agencies are a lack of curators and or collection, resulting in difficulties in producing new contents. This study aims to introduce cases in which museums with a specialty found their development plans in participatory exhibitions to be in line with a changing environment for museums. It is the participatory exhibition of the Court Museum of the Supreme Court which was designed by the cooperative project-collaborative development model suggested by Nina Simon among other models. This exhibition broadens the scope of the audience and served as an opportunity to change the previous direction of the museum. The agency came to understand that the operation of the museum centered on the audience, and afterwards, it started to consider the audience rather than the public relations of the agency from the phase of deciding the themes of the special exhibitions. Thus, this study intends to suggest the process of the participatory exhibitions of the Court Museum of the Supreme Court that can be an exemplary case to seek for the appropriate ways to create new contents.