S-542 Indomethacin aggravates the renal injury by inhibition of adenosine-medited renal protection in AKI

( Hyejung Kim ),( Sun-hee Kim ),( Mi Seon Kang ),( Park Seok Ju ),( Min Sung An ),( Ki Beom Bae ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1

Background: Ischemia-reperfusion injury (IRI) is a leading cause of acute kidney injury with high morbidity and mortality due to limited therapy. AKI emerges in various clinical settings and is complex with outcome linking oxidative stress, inflammation, and cell death. Therefore protection of AKI is still an unsolved problem. Indomethacin is generally known that it inhibits the production of prostaglandins through the inhibition both cyclooxygenase (COX) 1 and 2. Prostaglandins have a wide variety of effect such as regulation of vasodilation, inflammation, regeneration, pain, fever. Therefore effect of indomethacin in AKI is different according to injury model. We investigated whether indomethacin which inhibits the production of prostaglandins aggravate the renal injury in AKI mouse model. Methods: Male C57/BL6 mice (8-10 weeks old, weight 20~25 g) were used. Acute kidney injury is induced by bilateral kidneys pedicle clamping which were subjected to 20 min or 30 min at both kidneys. Mice were treated with indomethacin at before and after injury. Blood and kidney samples were collected at 24 hr after IRI. The expression level of creatinine, N-gal & Kim-1 were detected in serum. And the expression level of PGE2, cAMP and adenosine were detected in kidney. Kidney Injury score were measured by HE staining and TUNEL. Results: In bilateral AKI model, Serum NGAL level and creatinine level were significantly highest in indomethacin treated group compared to non-treated group (NGAL, p<0.05; creatinine, p<0.01). Indomethacin treated group showed significantly more necrosis and apoptosis compared to non-treated group. Furthermore, Indomethacine inhibited the production of prostaglandins, cAMP and adenosine. Conclusions: Indomethacin inhibits adenosine-mediated renal protection by inhibition of prostaglandin production in AKI. Therefore Indomethacin worsened renal injury by inhibition of prostaglandin production in AKI. * This research was supported by a grant of the Korea Health Technology R&D Project through the KHIDI, funded by the Ministry of Health & Welfare, Republic of Korea (grant number :H15C2212)

초소형 E-Mobility 디자인 형태의 색채 감성요소에 대한 연구

김혜정 ( Kim¸ Hyejung ),김인중 ( Kim¸ Injoong ) 한국공간디자인학회 2021 한국공간디자인학회논문집 Vol.16 No.7

(연구배경 및 목적) 한국의 온실가스 감축 목표는 2020년 30% 감축에서 2030년에는 배출량 전망치의 37% 감축이 이루어 져야 한다. 이는 자동차 산업 전반적으로 기회와 재앙을 한꺼번에 가져 왔다. 온실가스 문제는 스마트 시티의 일환인 모빌리티 플랫폼 시장을 앞당겼다. 모빌리티 사회가 대두되면서 고령자, 주부, 장애인, 1인 가구 등 고가의 차량을 운영할 여력이 없거나 상시 이용이 필요 없는 경우 활용할 수 있는 저비용의 차량을 찾고 있다. 모빌리티 사회는 나홀로차량 확대로 경제적인 이유로 초소형 전기차를 찾는 고객이 나날이 늘어나고 있다. 이러한 변화 속에서 고객 선택의 폭을 다양하게 할 시대요구를 반영한 디자인이 필요하다. (연구방법) 연구방법으로는 초소형 전기차는 용도에 따라 다양한 형태로 분석 되기 때문에 여기서는 초소형 전기차의 전기승용차에 한정 짓고자 한다. 디자인의 형태 분석에 따라 형태, 기능, 시스템으로 일반적으로 연구를 하지만 본 연구자는 연구의 질적 향상과 심도있는 연구를 위하여 형태에 초점을 두고 그 중에서 디자인형태 구성요소 중에서 형태, 점, 선, 면, 입체, 색등 다양한 자료가 있지만, 시지각적으로 가장 가독성이 높은 색채를 중점으로 연구하고자 한다. (결과) 본 연구에서는 국내의 초소형 전기차를 EK-001, EK-002, EK-003경우 주조색과 보조색, 강조색이 다양하게 포지션으로 배색된 것에 비하여 해외의 EO-004, EO-005, EO-006의 경우 주조색을 메인으로 두고 보조색, 강조색은 아주 적은 배색 비율을 가지고 있다. 따라서 향후 국내 초소형 전기차개발시 이러한 연구내용을 적용한 색채 계획이 수립 필요하다. (결론) 초소형전기차의 다양한 디자인형태는 실내외 색채 디자인, 공간 조명 디자인, 개인 맞춤형 색채 디자인 플랫폼 등을 비롯하여 외관 색채의 다양한 확장으로 새로운 비즈니스 모델을 창출되길 기대한다. 특히 해외 색채의 경우 사용자의 취향에 따라 다양하게 가변가능한 색채를 사용하고 있으며 기존 색채의 방향에서 벗어나 운전자 선택의 폭을 넓게 하고 있다. 하지만 국내는 해외의 색채계획 수립과 다르게 주조색, 보조색, 강조색 포지션이 명확하지 않은 단점이 있다. 이는 문화적, 민족성의 차이 일수도 있기 때문에 심도 있는 접근과 연구가 향후 필요하다. (Background and Purpose) Korea's greenhouse gas reduction goal has developed from a 30% reduction in its emission forecast by 2020 to a 37% reduction by 2030. This has created both opportunities and disasters in the automobile industry as a whole. The greenhouse gas problem has accelerated the mobility platform market, which is part of smart cities. With the emergence of a mobile society, demand for low-cost vehicles that can be used for people such as the elderly, housewives, the disabled, and single-person households, or people that do not need to use them all the time, is growing. In the age of mobility, the number of customers looking for ultra-small electric vehicles for economic reasons is increasing. Based on these social realities, a design that reflects the era of diversifying customer choices is needed. (Method) As a research method, ultra-small electric vehicles are analyzed in various forms depending on their application; in this study, we limit them to ultra-small electric vehicles. In general, many types of research are conducted by dividing design into form, function, and system according to the analysis of the form of the design. However, this research focuses on the form of design for improving research quality through an in-depth study. In addition, among the design type components, there are various data such as shape, dot, line, face, solid, and color, but this study focuses on the color with the highest visual readability. (Results) Therefore, in the case of EK-001, EK-002, EK-003, the casting color, auxiliary color, and highlight color were colored in various positions, whereas in the case of EO-004, EO-005, EO-006 overseas, the auxiliary color and highlight color had a very small color ratio. Therefore, the color plans that have been applied to the results of this study ought to be used when developing ultra-small electric vehicles in the future. (Conclusions) Various design forms of ultra-small electric vehicles are expected to create new business models by expanding exterior colors, including indoor and outdoor color design, spatial lighting design, and personalized color design platforms. In particular, in the case of overseas colors, various variable colors are used depending on the user's taste, and the driver's choice is broadened away from the direction of the existing color. However, unlike overseas color planning in Korea, there is a disadvantage in that the positions of casting, auxiliary, and highlighted colors are not clear. As this may represent cultural or ethnic differences, in-depth approaches and further research are needed in future.

Acupuncture suppresses kainic acid-induced neuronal death and inflammatory events in mouse hippocampus

Kim, Seung-Tae,Doo, Ah-Reum,Kim, Seung-Nam,Kim, Song-Yi,Kim, Yoon Young,Kim, Jang-Hyun,Lee, Hyejung,Yin, Chang Shik,Park, Hi-Joon Springer-Verlag 2012 JOURNAL OF PHYSIOLOGICAL SCIENCES Vol.62 No.5

<P>The administration of kainic acid (KA) causes seizures and produces neurodegeneration in hippocampal CA3 pyramidal cells. The present study investigated a possible role of acupuncture in reducing hippocampal cell death and inflammatory events, using a mouse model of kainic acid-induced epilepsy. Male C57BL/6 mice received acupuncture treatments at acupoint HT8 or in the tail area bilaterally once a day for 2?days and again immediately after an intraperitoneal injection of KA (30?mg/kg). HT8 is located on the palmar surface of the forelimbs, between the fourth and fifth metacarpal bones. Twenty-four hours after the KA injection, neuronal cell survival, the activations of microglia and astrocytes, and mRNA expression of two proinflammatory cytokines, interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), were measured in the hippocampus. Acupuncture stimulation at HT8, but not in the tail area, significantly reduced the KA-induced seizure, neuron death, microglial and astrocyte activations, and IL-1β mRNA expression in the hippocampus. The acupuncture stimulation also decreased the mRNA expression of TNF-α, but it was not significant. These results indicate that acupuncture at HT8 can inhibit hippocampal cell death and suppress KA-induced inflammatory events, suggesting a possible role for acupuncture in the treatment of epilepsy.</P>

Effects of HSP27 downregulation on PDT resistance through PDT-induced autophagy in head and neck cancer cells

KIM, JISUN,LIM, HAESOON,KIM, SANGWOO,CHO, HYEJUNG,KIM, YONG,LI, XIAOJIE,CHOI, HONGRAN,KIM, OKJOON Spandidos Publications 2016 Oncology Reports Vol.35 No.4

<P>We previously reported that photodynamic therapy (PDT) induces cell death in head and neck cancer through both autophagy and apoptosis. Regulation of cell death by autophagy and apoptosis is important to enhance the effects of PDT. Autophagy maintains a balance between cell death and PDT resistance. Downregulation of heat shock protein 27 (HSP27) induces PDT resistance in head and neck cancer cells. Furthermore, HSP70 regulates apoptosis during oxidative stress. However, the role of HSPs in PDT-induced cell death through autophagy and apoptosis is unclear. Therefore, in the present study, we investigated the effects of HSP27 and HSP70 on PDT-induced cell death of oral cancer cells through autophagy and apoptosis. Cancer cells were treated with hematoporphyrin at varying doses, followed by irradiation at 635 nm with an energy density of 5 mW/cm(2). We determined the changes in HSP expression by determining the levels of PARP-1 and LC3II in PDT-resistant cells. Furthermore, we assessed cell death signaling after downregulating HSPs by transfecting specific siRNAs. We observed that PDT decreased HSP27 expression but increased HSP70 expression in the head and neck cancer cells. Treatment of cells with LC3II and PARP-1 inhibitors resulted in upregulation of HSP70 and HSP27 expression, respectively. Downregulation of HSP27 and HSP70 induced cell death and PDT resistance through autophagy and apoptosis. Moreover, downregulation of HSP27 in PDT-resistant cells resulted in enhanced survival. These results indicate that the regulation of HSP27 and HSP70 plays a principal role in increasing the effects of PDT by inducing autophagic and apoptotic cell death.</P>

Ideal Blood Pressure in Patients With Atrial Fibrillation

Kim, Daehoon,Yang, Pil-Sung,Kim, Tae-Hoon,Jang, Eunsun,Shin, Hyejung,Kim, Ha Yan,Yu, Hee Tae,Uhm, Jae-Sun,Kim, Jong-Youn,Pak, Hui-Nam,Lee, Moon-Hyoung,Joung, Boyoung,Lip, Gregory Y.H. Elsevier 2018 JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY - Vol.72 No.11

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>The 2017 American College of Cardiology/American Heart Association (ACC/AHA) Guideline for High Blood Pressure in Adults redefined hypertension as systolic blood pressure (BP) ≥130 mm Hg or diastolic BP ≥80 mm Hg. The optimal BP for patients with atrial fibrillation (AF) is uncertain.</P> <P><B>Objectives</B></P> <P>The goal of this study was to investigate the impacts of the 2017 ACC/AHA guideline and to determine the ideal BP threshold for the management of high BP in patients with AF.</P> <P><B>Methods</B></P> <P>This study analyzed data for 298,374 Korean adults with oral anticoagulant–naive, nonvalvular AF obtained from the National Health Insurance Service database from 2005 to 2015.</P> <P><B>Results</B></P> <P>According to the Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation and Treatment of High Blood Pressure guideline, 62.2% of the individuals in our sample had hypertension. After applying the 2017 ACC/AHA guideline, 79.4% had hypertension, including 17.2% with newly redefined hypertension (130 to 139/80 to 89 mm Hg). Those with newly redefined hypertension had greater risks of major cardiovascular events (hazard ratio: 1.07; 95% confidence interval: 1.04 to 1.10; p < 0.001), ischemic stroke, intracranial hemorrhage, and heart failure admission, compared with nonhypertensive patients (<130/80 mm Hg). Among patients with AF undergoing hypertension treatment, patients with BP ≥130/80 mm Hg or <120/80 mm Hg were at significantly higher risks of major cardiovascular events than patients with BP of 120 to 129/<80 mm Hg.</P> <P><B>Conclusions</B></P> <P>Patients with AF and newly redefined hypertension according to the 2017 ACC/AHA guideline were at higher risk of major cardiovascular events, suggesting that the new BP threshold is beneficial for timely diagnosis and intervention. BP of 120 to 129/<80 mm Hg was the optimal BP treatment target for patients with AF undergoing hypertension treatment.</P> <P><B>Central Illustration</B></P> <P>[DISPLAY OMISSION]</P>

Enhanced NMDA receptor-mediated synaptic transmission, enhanced long-term potentiation, and impaired learning and memory in mice lacking IRSp53.

Kim, Myoung-Hwan,Choi, Jeonghoon,Yang, Jinhee,Chung, Woosuk,Kim, Ji-Hyun,Paik, Sang Kyoo,Kim, Karam,Han, Seungnam,Won, Hyejung,Bae, Young-Soo,Cho, Suk-Hee,Seo, Jinsoo,Bae, Yong Chul,Choi, Se-Young,Kim The Society 2009 The Journal of neuroscience Vol.29 No.5

<P>IRSp53 is an adaptor protein that acts downstream of Rac and Cdc42 small GTPases and is implicated in the regulation of membrane deformation and actin filament assembly. In neurons, IRSp53 is an abundant postsynaptic protein and regulates actin-rich dendritic spines; however, its in vivo functions have not been explored. We characterized transgenic mice deficient of IRSp53 expression. Unexpectedly, IRSp53(-/-) neurons do not show significant changes in the density and ultrastructural morphologies of dendritic spines. Instead, IRSp53(-/-) neurons exhibit reduced AMPA/NMDA ratio of excitatory synaptic transmission and a selective increase in NMDA but not AMPA receptor-mediated transmission. IRSp53(-/-) hippocampal slices show a markedly enhanced long-term potentiation (LTP) with no changes in long-term depression. LTP-inducing theta burst stimulation enhances NMDA receptor-mediated transmission. Spatial learning and novel object recognition are impaired in IRSp53(-/-) mice. These results suggest that IRSp53 is involved in the regulation of NMDA receptor-mediated excitatory synaptic transmission, LTP, and learning and memory behaviors.</P>

GIT1 is associated with ADHD in humans and ADHD-like behaviors in mice

Won, Hyejung,Mah, Won,Kim, Eunjin,Kim, Jae-Won,Hahm, Eun-Kyoung,Kim, Myoung-Hwan,Cho, Sukhee,Kim, Jeongjin,Jang, Hyeran,Cho, Soo-Churl,Kim, Boong-Nyun,Shin, Min-Sup,Seo, Jinsoo,Jeong, Jaeseung,Choi, S Nature Publishing Group, a division of Macmillan P 2011 Nature medicine Vol.17 No.5

Attention deficit hyperactivity disorder (ADHD) is a psychiatric disorder that affects ??% of school-aged children; however, the mechanisms underlying ADHD remain largely unclear. Here we report a previously unidentified association between G protein??coupled receptor kinase??interacting protein-1 (GIT1) and ADHD in humans. An intronic single-nucleotide polymorphism in GIT1, the minor allele of which causes reduced GIT1 expression, shows a strong association with ADHD susceptibility in humans. Git1-deficient mice show ADHD-like phenotypes, with traits including hyperactivity, enhanced electroencephalogram theta rhythms and impaired learning and memory. Hyperactivity in Git1<SUP>??/??</SUP> mice is reversed by amphetamine and methylphenidate, psychostimulants commonly used to treat ADHD. In addition, amphetamine normalizes enhanced theta rhythms and impaired memory. GIT1 deficiency in mice leads to decreases in ras-related C3 botulinum toxin substrate-1 (RAC1) signaling and inhibitory presynaptic input; furthermore, it shifts the neuronal excitation-inhibition balance in postsynaptic neurons toward excitation. Our study identifies a previously unknown involvement of GIT1 in human ADHD and shows that GIT1 deficiency in mice causes psychostimulant-responsive ADHD-like phenotypes.

수학문장제 문제해결력 검사 타당화 연구: 학습장애 위험군 선별을 중심으로

김동일(Kim, Dongil),고혜정(Koh, Hyejung),신재현(Shin, Jaehyun),김이내(Kim, Ienai),김우리야(Kim, Wooliya),김붕년(Kim, Boongnyun),이기정(Lee, Kijyung) 한국열린교육학회 2013 열린교육연구 Vol.21 No.1

본 연구는 학생들의 수학문장제 문제해결력을 정확히 예측할 수 있고 보다 직접적으로 측정할 수 있는 검사도구로서 개발된 ‘수학문장제 문제해결력 검사’의 신뢰도와 타당도를 검증하고자 하였다. 본 연구에서는 ‘수학문장제 문제해결력 검사’의 타당도를 구성타당도, 준거타당도, 그리고 변별타당도 측면에서 살펴보았다. 그 결과, 문항 간 내적 합치도는 전체 .898로 높은 편이었고, 타당도의 경우, 요인의 일차원성이 확보되어 본 검사가 단일요인을 측정하고자 함이 입증되었으며, 기초학습기능 평가체제-읽기 및 수학과의 준거타당도가 양호한 것으로 나타났다. 또한 읽기와 수학의 한 영역 또는 두 영역 모두에 심각한 문제를 지닌 읽기-수학 공존 학습장애의 위험을 보이는 저성취 아동들을 적절히 변별해내는 데에도 효과적인 검사도구임이 밝혀졌다. 따라서 ‘수학문장제 문제해결력 검사’는 교육현장에서 수학능력을 정형화된 연산 정확도로만 측정하기보다 수학교육의 궁극적인 목표인 문제해결능력을 점검하는데 유용하게 사용될 수 있을 것으로 기대된다. The purpose of this study is to develop a ‘curriculum-based mathematical word problem solving measurement’ which can predict with accuracy and measure directly mathematical word problem solving ability, and to verify the reliability and validity of that measurement. The study examined the reliability and validity of a ‘curriculum-based mathematical word problem solving measurement.’ In terms of validity, factor analysis was conducted and both criteria validity and discriminant validity were examined. The results showed relatively high internal consistency coefficients across all items with Cronbach’s α coefficient which was .898. In the result of factor analysis, it yielded only one principle component and it was significantly correlated with BASA-Math and maze test of BASA-Reading. In addition, by examining the discriminant validity, it was revealed that significant differences in mathematical word problem solving scores were found among the children with reading difficulties, math difficulties, and math-reading comorbid difficulties. Therefore, a 'curriculum-based mathematical word problem solving measurement' would be able to provide a more reliable information of mathematical problem solving ability rather than simple calculation tests, and it is expected to be used appropriately in school settings.

석유화학 공장 사무실 공기질과 근로자 자각 증상과의 연관성에 관한 연구

김기연(Kiyoun Kim),김혜정(Hyejung Kim),김현수(Hyunsoo Kim),김치년(Chinyon Kim),원종욱(Jonguk Won),노영만(Youngman Roh),노재훈(Jaehoon Roh) 한국실내환경학회 2006 한국실내환경학회지 Vol.3 No.3

This research was performed with the subject of 111 offices in a chemical plant of Korea. Airborne concentration level of carbon dioxide, carbon monoxide, formaldehyde and total suspended particulates in the office rooms were measured along with temperature and relative humidity. Simultaneously, general characteristics and subjective health symptoms of 500 office workers were investigated through a questionnaire consisting of the five point weighting method : 0 = not at all, 1 = a little bit, 2 = moderately, 3 = quite a bit and 4 = extremely. Results showed that the mean values for temperature and relative humidity in investigated office rooms were 22.2℃ and 34.3%, respectively. The concentrations of formaldehyde, PM10, carbon dioxide and carbon monoxide were 0.17 ppb, 28.8 ㎍/㎥, 0.95 ppm and 424 ppm, respectively. 56.4% of all the workers investigated replied with a sense of fatigue or weariness (mean=2.23), drowsiness or languidness (mean=2.22), sneezing (mean=2.11) and ophthalmia oreye fatigue (mean=2.07). Female workers, non-smokers, non-drinkers, workers who do shift more than nine hours a day, and workers in poor health experienced more subjective symptoms than other workers (p<0.05). Subjective symptoms of office workers were significantly associated with the following items of office characteristics: the purchase date of office equipment, the change of office furniture, the use of paint, smoking in the office, ventilation condition, the use of an individual air-conditioner, the use of a fax machine, the degree of office repair, the proportion of workers per office, the degree of satisfaction in office environment, and the operation hours of the air-conditioner. Also, subjective symptoms were positively correlated with indoor environmental factors such as relative humidity, carbon dioxide level, level of PM10, and formaldehyde (p<0.05). In conclusion, office characteristics and air quality in a chemical plant affected subjective health symptoms of office workers. Thus, in order to improve the health of workers, to enhance work efficiency, and to establish a better office environment, air quality control in office rooms by optimal ventilation, adequately occupied number of workers in one office, and maintenance of office equipment should be fulfilled thoroughly.

Individualized Acupuncture for Symptom Relief in Functional Dyspepsia: A Randomized Controlled Trial

Ko, Seok-Jae,Kuo, Braden,Kim, Seul-Ki,Lee, Hyangsook,Kim, Jinsung,Han, Gajin,Kim, Juyeon,Kim, Song-Yi,Jang, Seungwon,Son, Jiyoung,Kim, Minji,Lee, Hyejung,Yeo, Inkwon,Joo, Kwang Ro,Park, Jae-Woo Mary Ann Liebert 2016 Journal of Alternative & Complementary Medicine Vol.22 No.12