Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Stain improvement in the white button mushroom ‘Seolgang’ and its varietal characteristics in Agaricus bisporus

Byung-Joo Lee,Mi-Ae Lee,Yong-Gyun Kim,Kwang-Won Lee,Yong-Pyo Lim,Byung-Eui Lee,Ho-Yeon Song 한국버섯학회 2012 한국버섯학회지 Vol.10 No.4

The button mushroom (Agaricus bisporus) is one of the most widely cultivated important edible mushroom species. In the breeding of new button mushroom, ‘Seolgang’ was developed by crossing two monokaryons ‘CM020913-27’ and ‘SSU423-31’. Because of the secondarily homothallism, only a small percentage of the basidia produce 3 or 4 spores, which are mostly haploid (n) and do not fruit. Single spore cultures derived from these types of spores produce a vegetative mycelium that also contain a variable number of genetically identical nuclei per cell called monokaryon. The lack of clamp connections between monokaryon and dikaryon required a series of mycelial culture and fruiting test. After crossing, hybrids were cultivated on a small scale and on a commercial scale at a farm. For this, the spawn was made by a commercial spawn producer and the spawned compost by a commercial compost producer. Mycelial growth of ‘Seolgang’ on CDA was better at 20℃ and 25℃ when it was compared with that of ‘505 Ho’. The mature cap shape of new strain ‘Seolgang’ is oblate spheroid and the immature cap shape is round to oblate spheroid. The cap diameter was 41.2 mm on average. In comparison with white strain ‘505 Ho’, the strain had a yield that was 9% higher. It produced fruiting bodies which had a higher weight on average per fruiting body and were 19% firmer with a good shelf life. Days of fruiting body were 3-4 days later than those of ‘505 Ho’. The physical characteristics such as elasticity, chewiness, adhesiveness were better than that of ‘505 Ho’. Genetic analysis of the new strain ‘Seolgang’ showed different profiles compared to ‘505 Ho’, CM02913-27, SSU413-31, when RAPD primers A02 and O04 were used.

그람음성 세균에 대한 나노실버의 항균효과

송호연,고광균 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Silver is one of the most universal anti-microbial substances. To improve the anti-microbial activity of silver, we successfully produced nanosilver by nano-technology. We examined the anti-microbial activity of nanosilver for several Gram negative pathogenic bacteria(Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi). Most bacteria were controlled within 1 ppm of nanosilver witin 1 hour. The mechanism of anti-microbial effects was explored by SEM and TEM. The severe morphologic changes, irregular cell wall, increased electron density of cytoplasm, and plasmolysis, were observed. From the result, nanosilver will be available as an antibiotics alternative.

RF-magnetron 스퍼터링에 의해 제조된 HAp와 HAp-Ag 복합재료의 미세구조와 In-vitro 연구

송호연,이희정,고광균 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Microstructure and in-vistro study of RF-magnetron sputtered HAp and HAp-Ag composite films on the ZrO_(2) substrate were investigated using XPS, SEM, AFM and HR-TEM techniques. The homogenous and dense films about 0.8 ㎛ in thickness were successfully obtained and they were comprised with nano-crystalline grains about 30 nm in diameter. Micro cracks were not found at the interfaces between HAp and ZrO_(2) substrate and also on theire films. Without the dependency of Ag addition the fracure mode of HAp-Ag films showed a transgranular type. Due to the excellent bioaffinity, osteoblast MG-63 cells wre grown well on the HAp and HAp-Ag films with dense and healthly morphology, although the growth rate of cells on the HAp-Ag film was slower than that of the HAp film.

사람의 혈관 내피세포에 대한 Salmonella 균종의 감염

송호연,고광균,최순룡 순천향대학교 1994 논문집 Vol.17 No.4

In order to elucidate the mechanism of injury to endothelial cell of blood vessels in typhoid fever, Salmonella typhi was inoculated in cultured human endothelial cells. Endothelial cells were prepared from the umbilical vein that was freshly obtained from human umbilical cord by collagenase treatment. After screening of cultured endothelial cells through the immunofluorescent antibody technique, ultrastructural change of cytopathic Aeffect, obtained by lectron microscopy, showed typical progress of pathogenesis caused by S. typhi; adherence, endocytosis, intracellular replication and release of microorganism. These patholgical changes led to cellular disruption and final death of endothelial cells. Endothelial cells of vascular system found to be the site of amplification as well as the target organ of S. typhi, which could play important role in hematogenous spread of this microorganism and also in pathologic sign or symptom in typhoid fever. An experimental infection of S. typhi in cultured human endothelial cells found to be an excellent and valuable virulence assay system. The above ability of S. typhi to proliferate and lead to endothelial cell death in cultured human endothelial cells confirmed to be a factor of endovascular disorder of typhoid fever such as rose spot on abdomen of patients.

대식세포내 결핵균 증식에 미치는 Prothymosin alpha Antisense Oligonucleotide의 영향

송호연 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Mycobactetium tuberculosis remains one of the world's most important pathogens. It is able to grow and survive within macrophage. I recently have identified the up-regulation of prothymosin α in THP-1 cells infected with Mycobacterium tuberculosis H_(37)Rv(virulent strain) using the differential display reverse transcriptase polymerase chain reaction(DD RT-PCR) and nothern blot. The purpose of this study was to identify the effect of prothymosin α antisense oligonucleotide in multiplication of Mycobacterium tuberculosis in macrophages. I have confirmed the over-expression of prothymosin α in THP-1 cells infected with M. Tuberculosis H_(37)Rv 48 hours postinfection. Also I found that the prothymosin α antisense oligonucleotide induced the replication of mycobacteria in macrophages infected with M. tuberculosis H_(37)Rv. This result suggests that the over-expression of prothymosin α protein is induced by infection of a virulent mycobacteria and it might be inhibit the replication of mycobacteria in macrophages.

생체세라믹 분말이 뼈모세포의 증식에 미치는 영향

송호연,고광균 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

The important cause of re-operation in patients who received total hip arthroplasty is wear-debries-mediated osteolysis in bone-implant interface. We observed the effects of Al_(2)O_(3) and Ag-coated alumina (Al_(2)O_(3)-Ag) particles on the growth and morphologic changes of osteoblast. The results were compared with that of osteoblast cultured on Al_(2)O_(3), HAp-coated Al_(2)O_(3) and HAp-Ag composite Al_(2)O_(3) thin films. According to the effects of the particles, the cell number was decreased and the morphology of osteoblast was changed considerably compared with control. However, the proliferation of osteoblast cultured on HAp and HAp-Ag thin films were increased than control. These results suggest that the shape (particles) of material as well as characteristics of material itself may give a significant effect on the behavior of cell.

결핵균 감염에 따른 큰 포식세포의 Ferritin H chain 유전자 발현

송호연,고광균 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The purpose of this study was to identify macrophage genes regulated by infection of mycobacteria. Mycobacterium tuberculosis H37Rv (ATCC 27294) was provided from Korean National Tuberculosis Association. We have used a differential display RT-PCR to isolated cDNAs corresponding to transcripts that induced in THP-1 cells infected with M. tuberculosis. Among several differentially expressed trascripts, one clone, designated as 1-TC8 was 322 bp and was identical to human ferritin heavy(H) chain gene. Northern blot analysis confiremed that ferritin H chain gene has been markedly over-expressed in monocytic THP-1 cells infected with live Mycobacterium tuberculosis.

결핵균 감염에 의한 대식세포에서의 TNFα 유전자 발현 증가

송호연 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Mycobactetium tuberculosis is capable of growing and survival dormant for a long time within macrophages. This might be due to a balance between antimicrobial activity of host cell and pathogencity of M. tuberculosis. This study examined gene regulation in human monocyte derived THP-1 cell infected with mycobacteria. Gene expression was evaluated by differential display RT-PCR and was compared to uninfected cells. Results were confirmed by real time quantitative RT-PCR(TaqMan). Among many up or down-regulated clones, 25 colnes were sequenced and compared with known genes on GenBank. One of over-expressed clones from THP-1 cells infected with mycobacteria was identical to human tumor necrosis factorα(TNFα). In particular, the expression of TNFα was highly over-expressed in THP-1 cells infected with M. tuberculosis H_(37)Ra(avirulent) than H_(37)Rv(virulent). This may in fact be a mycobactetial virulence tactic.

Vibrio cholerae균 분리를 위한 새로운 분별배지

송호연,고광균 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

The purpose of this experiment is to develop a new differential medium for Vibrio cholera. The TCBS agar medium is used commonly for the identification of vibrio species, but it contains some inhibitors against other bacteria to increase the selectivity for vibrio species. So we developed the BYS agar medium as a new differential medium for V. cholera. We compared the colony forming unit(CFU) between BYS and TCBS using streak culture method and micro-pour plate method(100 ㎕). The CFU count was more than thirty times higher in BYS agar medium than in TCBS in both methods. These results suggest that BYS agar medium is a differential medium better than TCBS agar medium for the identification of V. cholera.