A Comparison of Lyophilized Amniotic Membrane with Cryopreserved Amniotic Membrane for the Reconstruction of Rabbit Corneal Epithelium

Ahn Jae-Il,Jang In-Keun,Lee Doo-Hoon,Seo Young-Kwon,Yoon Hee-Hoon,Shin Youn-Ho,Kim Jae-Chan,Song Kye-Yong,Lee Hee-Gu,Yang Eun-Kyung,Kim Ki-Ho,Park Jung-Keung The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.3

Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn's AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an ex-plantation method. The corneal epithelium could be reconstructed by culturing the third­passage corneal epithelial cells on the AM. A lyophilized amniotic membrane (LAM) has a higher rate of graft take, a longer shelf life, is easier to store, and safer, due to gamma irradiation, than a (AM. The corneal epithelium reconstructed on the LAM and (AM, supported by the two­Teflon rings, was similar to normal corneal epithelium. However, the advantages of the LAM over that of the (AM make the former more useful. The reconstruction model of the corneal epithelium, using AM, is considered as a good in vitro model for transplantation of cornel epithelium into patients with a severely damaged cornea.

TV 드라마 속 "중년 여배우들"의 패션과 미용요소 분석

임희경(Hee Kyung Im),안금희(Gum Hee Ahn) 한국디자인문화학회 2011 한국디자인문화학회지 Vol.17 No.2

본 연구는 ``미워도 다시한번 2009``(2009년)의 전인화와 최명길, ``즐거운 나의 집``(2010년)의 황신혜, ``마이더스``(2011년)의 김희애를 중심으로 TV드라마 속 중년 여배우들의 패션과 미용요소를 살펴봄으로써 패션의 유행 변화를 파악하고 예측 및 분석하는데 기초자료를 제공하여 중년 여성의 패션과 미용 산업에 폭넓은 발전에 도움이 되고자 하는 데 목적을 두었다. 연구방법은 성격분석 및 의상, 헤어스타일, 메이크업, 액세서리 등 드라마 공식 홈페이지 스틸 컷 사진과 드라마 방영 화면을 캡처한 사진 40장을 수집하여 실증적 분석도구로 활용하여 패션과 미용요소들을 분석하였다. 구체적인 사진 선정 방법은 전문가 집단을 대상으로 미용관련학과 교수 2인, 미용학과 대학원생 6인 총 8인을 구성해 패션과 미용요소가 대표성 있는 사진을 선별하였다. 또한 논문의 이론적 배경을 뒷받침하기 위해 인터넷과 문헌연구로는 국내 관련 서적, 논문, 간행물, 문헌자료, 인터넷을 중심으로 논문의 타당성을 증명하였다. 연구 범위는 1장에서 TV드라마의 개념 및 고찰을 하였고, 2장에서는 TV 드라마에 나타난 패션과 미용요소를 2000년대 전반에 걸쳐 알아봄으로 그것들이 단순히 시각적으로 시청자들을 자극하는 것이 아니라 대중들에게 자연스럽게 유행을 주도하고 창조하는 문화적 아이콘으로 선두적 역할을 하고 있다라는 것을 알아본다. 3장인 결과에서는 각 TV드라마에 나타난 중년 여배우들의 패션과 미용요소들을 구체적으로 살펴보았다. ``미워도 다시한번 2009``의 전인화의 패션은 팜므파 탈룩, 미용요소는 스모키 메이크업과 미디엄 레이어길이의 헤어스타일과 업스타일을 하였다. 최명길의 패션은 CEO룩, 미용요소는 매트한 피부질감의 깨끗한 메이크업을 하였으며 헤어스타일은 보브컷을 하였다. ``즐거운 나의 집``의 황신혜는 락시크룩의 패션과 스모키 메이크업과 롱 웨이브헤어스타일을 선보였다. ``마이더스``의 김희애는 패션은 노블레스룩, 미용요소는 내추럴 메이크업과 층이 없는 단발머리 스타일이었다. 4장에서는 결론부분으로 TV드라마 속 중년 여배우들의 패션과 미용요소는 극의 내용을 표현하기도 하지만 그 시대의 유행 트렌드를 반영하기도 하고 중년여성뿐 만아니라 다양한 연령대의 대중들에게 모방심리를 자극하여 유행을 창조하는 등 사회적으로 미치는 영향력이 크다고 할 수 있겠다라고 마무리하였다. The purpose of this study is to help a broad development in the middle-aged women`s fashion and beauty industries by providing basic data to examine, predict and analyze fashion changes throughout fashion and beauty elements of the middle-aged actress in TV drama focusing on Jeon, In-Hwa and Choi, Myoung-Gil of ``Although Is Hateful Again 2009`` (in 2009), Hwang, Sin-Hye of ``Sweet My Home`` (in 2010) and Kim Hee-Ae of ``Midas`` (2011). As a method of the research, the elements of fashion and beauty were analyzed by collecting captured 40 pictures from drama official website steel-cut picture and drama screen as an empirical analysis tool such as analysis of the characteristic, costumes, hairstyles, makeup, accessories. The method to select a specific picture is to select representative fashion and beauty elements picture with the target group of experts such as 2 cosmetic professors, 6 fashion graduate students-total 8 people. In addition, the validity of the thesis was demonstrated focusing on the domestic-related books, papers, publications, literature, internet regarding Internet and Literature Review to support a theoretical background of thesis. Scope of the research is the concept and review of TV dramas in chapter 1 and fashion and beauty elements on TV drama throughout the 2000s plays a leading role to lead and create trend naturally to the public as a cultural icon, not to simply stimulate the audience visually in chapter 2. Fashion and beauty elements of middle-aged actress on TV drama were examined in chapter 3. The fashion of Jeon, In-Hwa of ``Although Is Hateful Again 2009`` (in 2009) is Femme fatale and cosmetic elements are smoky makeup and medium-length hair style and up style. The fashion of Choi, Myoung-Gil is CEO look and cosmetic elements are clean makeup of matt skin texture and the hair style is Bob cut. Hwang, Sin-Hye of ``Sweet My Home`` (in 2010) showed Rockchic look fashion, smoky makeup and long wave hairstyles. The fashion of Kim Hee-Ae of ``Midas`` (2011) is Nobless look and beauty elements are natural makeup and pageboy. In chapter 4, as a result, the fashion and beauty elements of middle-aged actress in a TV drama present information of the drama, but reflect the trend of the times and create the trend by stimulating imitation mind to the public with various ages as well as middle-aged women, thus it impacts on the society heavily.

뇌성마비아의 모발내 중금속 함량 분석

안경희,김희상,유창현,윤동환,홍장혁,유승돈,이준원 경희대학교 2001 慶熙醫學 Vol.17 No.1

흰쥐 뇌줄기에 분포하는 Nitric Oxide Synthase 신경세포에 관한 면역조직화학적 연구

나준희,허영범,박찬,김정혜,유진화,안희경,이원규 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.2

환경침해를 이유로 한 유지청구의 법적 근거

안경희 안성산업대학교 2006 論文集 Vol.38 No.-

Das Thema der zivilrechtliche Abwehranspruchsgrundlage fu¨r beeintra¨chtigende Umwelteinwirkungen ist einerseits seit langem vorhanden und bekannt, andererseits hat die Thematik naheliegenderweise erst in den vergangenen 20 Jahren die riesige Bedeutung erlangt, die dem Problemkreis heute zukommt. Bei aktiver Abwehr der Immissionen handelt es sich um Abwehrrecht des Gesto¨rten und Einwendungen des Sto¨rers. Die Anspruchsgrundlage des Abwehranspruchs ist § 214 BGB. Danach kann der Eigentu¨mer die Beseitigung jeder Beeintra¨chtigung seines Eigentums außer einer Besitzentziehung oder Besitzvorenthaltung fordern. Hiergegen wiederum kann der Sto¨rer die Einwendungen gema¨ß § 217 BGB vorbringen, der Gestörte habe die Beeintra¨chtigung zu dulden.

뇌성마비아의 모발내 망간 분석 연구

김희상,김종영,안경회 大韓再活醫學會 1999 Annals of Rehabilitation Medicine Vol.23 No.3

Reconstruction of Rabbit Corneal Epithelium on Lyophilized Amniotic Membrane Using the Tilting Dynamic Culture Method

Ahn, Jae-Il,Lee, Doo-Hoon,Ryu, Yang-Hwan,Jang, In-Keun,Yoon, Mun-Young,Shin, Youn Ho,Seo, Young-Kwon,Yoon, Hee-Hoon,Kim, Jae-Chan,Song, Kye-Yong,Yang, Eun-Kyung,Kim, Ki-Ho,Park, Jung-Keug Blackwell Publishing Inc 2007 Artificial Organs Vol.31 No.9

<P>Abstract: </P><P>Rabbit corneal epithelium was reconstructed using tilting dynamic culture with a self-manufactured, amniotic membrane (AM) supporter and a lyophilized amniotic membrane (LAM). Rabbit corneal epithelial (RCE) cells were cultured and cryopreserved after isolation from the limbus. The second- and third-passage RCE cells were plated onto the epithelial side of the LAM of Ahn's AM supporter. Two days later, the air–liquid interface culture was maintained with third-passage RCE cells for 6 days and second-passage corneal epithelial cells for 9 days. The average viability of thawed RCE cells, assessed using trypan blue dye exclusion, was 77.42%. The reconstructed corneal epithelium was characterized by histological (hematoxylin and eosin) and immunohistochemical staining (proliferating cell nuclear antigen) for light microscopy, and by reverse transcriptase-polymerase chain reaction, glucose assay, and transmission electron microscopy. The basal layer of the reconstructed corneal epithelium was well formed, and the epithelium was tightly constructed due to the increase in cell proliferation and differentiation caused by the tilting dynamic culture, as opposed to static culture. Tilting dynamic culture was useful for the reconstruction of the epithelium using easily damaged epithelial cells and resulted in more stratum cell layers. Moreover, cytokeratin (CK3) mRNA expression in tilting dynamic cultured third-passage RCE cells seeded onto AM was greater than in static cultured third-passage RCE cells. The morphology of the reconstructed corneal epithelium on LAM by tilting dynamic culture for 9 days resembled that of the skin epidermis. This was thought to be because the tilting dynamic culture not only accelerated the proliferation and differentiation of cells by physical or mechanical stimulation, but also ensured that the supply of medium was delivered to the basal cells more efficiently. Thus, the reconstruction of the corneal epithelium using LAM and tilting dynamic culture was considered to be a good in vitro model for autologous or allogeneic transplantation of corneal epithelium and skin epidermis in patients with damaged epithelia. </P>

요추부 경막외 스테로이드 주사에서 경추궁(經椎弓)법과경추간공(經椎間孔)법의 효과

김희상,홍장혁,안경회,김용걸 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.4

흉추부 신경병증에서 체성감각 유발전위검사의 임상적 유용성

안경회,오진주,김희상,이종하,정용설 대한근전도전기진단의학회 2003 대한근전도 전기진단의학회지 Vol.5 No.1

골다공성 척추 압박 골절 환자에서 시행한후관절 주사술의 효과 비교

김희상,유승돈,안경회 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.3