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토양소독액 자동분사 제어를 위한 분사 특성에 대한 연구 Ⅱ - 실험
유한주 ( Hanju Yoo ),오주선 ( Jooseon Oh ),박두산 ( Tusan Park ),심성보 ( Sungbo Shim ) 한국농업기계학회 2021 한국농업기계학회 학술발표논문집 Vol.26 No.2
토양 병해충은 밭작물 생산성 저하의 원인이 되므로, 윤작 혹은 토양 소독을 통해 적절한 토양 관리를 실시하고 있다. 윤작은 2가지 이상의 작물을 돌려가면서 농사를 짓는 방법으로 토지 이용도를 높이고, 반복 재배에도 균형 잡힌 토질을 유지할 수 있으나, 국내 농가 규모에는 부적절한 방식으로 널리 사용되지 못하고 있다. 토양 소독 방식 중 열처리 방식은 비용이 많이 들고 사용되는 장치 자체가 크므로, 상대적으로 재배 규모가 큰 환경에 적합한 방식으로 분류된다. 화학 약품을 살포하는 방식은 지중에 소독액을 살포하고 비닐로 덮어 훈증ㆍ소독하는 방법으로, 경제적이고 소규모 농업에도 적합하다. 그러나, 작업자가 소독액에 직접 노출되므로 작업자 안전과 작업 편의를 위해, 경운ㆍ소독액 분사ㆍ비닐피복이 동시에 진행할 수 있는 농기계 자동화 연구가 진행되고 있다. 본 논문에서는, 소독액 권장 도포량을 따르며 트랙터 차속에 따라 분사량이 제어되는 장치를 개발을 위해, 제어 변수를 설정하고 변수 간 특성을 확인하는 실험적 연구 결과를 제시한다. 제어 변수로는 트랙터 차속, 제어 모터 회전속도에 따른 분사 유량-분사 압력과 노즐 경으로 설정하였으며, 실험 결과는 분사량 자동제어 알고리즘 개발을 위한 기초 데이터로 활용할 것이다.
Suppression of melanogenesis by ebastine, the H1 antagonist, in human melanocytes
( Young Jae Kim ),( Hanju Yoo ),( Hyoung Min Na ),( Woo Jin Lee ),( Youngsup Song ),( Sung Eun Chang ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.1
Background: Histamine is involved in cutaneous melanogenesis, and H2-receptor antagonists were reported to inhibit melanogenesis through modulation of the cyclic adenosine monophosphate/protein kinase A axis. However, H1-receptor antagonists have not well been investigated for regulation of melanogenesis. Objectives: We examined whether ebastine, a commonly used H1-receptor antagonist for the treatment of itchy dermatosis may inhibit melanogenesis. Methods: In normal human melanocytes (NHM), Mel-ab cells, and ex vivo skin cultures were examined for cell viability. Melanin content, tyrosinase activity, and levels of MITF, DCT, TRP1, tyrosinase and membranous protein kinase C (PKC)-βII were measured after treatment of ebastine. Ex vivo skin culture were subject to melanin index and melanocytic viability. Results: Ebastine decreased melanin content and tyrosinase activity in a dose-dependent manner without affecting cell viability. Mechanistically, ebastine reduced membranous expression of PKC-βII. In ex vivo human skin, ebastine decreased melanosome deposition with well preserved epidermal melanocytes. Conclusion: Further clinical investigations are required; still, our findings imply that anti-melanogenic action of ebastine could be valuable for the management of skin hyperpigmentary disorders which are often associated with itchy dermatosis.
NEDD4L limits cAMP signaling through ubiquitination of CREB‐regulated transcription coactivator 3
Kim, Yo‐,Han,Yoo, Hanju,Hong, A‐,Reum,Kwon, Minseo,Kang, Sang‐,Wook,Kim, Kyunggon,Song, Youngsup Federation of American Society for Experimental Bi 2018 The FASEB Journal Vol.32 No.7
<P>The transcription factor cAMP-responsive element-binding protein (CREB) is involved in a variety of physiologic processes. Although its activity appears to be largely correlated with its phosphorylation status, cAMP-mediated dephosphorylation and the subsequent nuclear migration of the CREB-regulated transcription factors (CRTCs) are required to stimulate CREB transcriptional activity. Among the 3 identified mammalian homologs of CRTCs, CRTC3 has been shown to be expressed predominantly in adipose tissues in response to catecholamine signals that regulate lipid metabolism. Here, we show that prolonged cAMP signaling down-regulates CRTC3 in a proteasome-dependent manner and that neural precursor cell-expressed developmentally down-regulated gene 4-like (NEDD4L), a specific ubiquitin ligase for CRTC3, is responsible for this process. By recognizing the PY motif of CRTC3, NEDD4L interacts with CRTC3 and promotes its polyubiquitination. Interaction between NEDD4L and CRTC3 is further boosted by cAMP signaling, and this enhanced interaction appears to be dependent on the cAMP-mediated phosphorylation of NEDD4L at the Ser448 site. Furthermore, we show that food withdrawal stimulates NEDD4L phosphorylation in mice, which then show a decrease of adipose tissue CRTC3 protein levels. Together, these results suggest that NEDD4L plays a key role in the feedback regulation of cAMP signaling by limiting CRTC3 protein levels.Kim, Y.-H., Yoo, H., Hong, A.-R., Kwon, M., Kang, S.-W., Kim, K., Song, Y. NEDD4L limits cAMP signaling through ubiquitination of CREB-regulated transcription coactivator 3.</P>
Kim, Songhui,Yoon, Youngwoon,Lee, Hanju,Choi, Ah Reum,Jung, Kwang-Hwan,Babajanyan, Arsen,Abrahamyan, Tigran,Yoo, Hyungkeun,Lee, Jung-Ha,Cha, Deokjoon,Berthiau, Gerard,Friedman, Barry,Lee, Kiejin Wiley 2013 Journal of biophotonics Vol.6 No.2
<P>We study the opto-electrical properties of Natronomonas pharaonis sensory rhodopsin II (NpSRII) by using a near-field microwave microprobe (NFMM) under external light illumination. To investigate the possibility of application of NFMM to biological macromolecules, we used time dependent properties of NPSRII before/after light activation which has three distinct states - ground-state, M-state, and O-state. The diagnostic ability of NFMM is demonstrated by measuring the microwave reflection coefficient (S(11)) spectrum of NpSRII under steady-state illumination in the wavelength range of 350-650 nm. Moreover, we present microwave reflection coefficient S(11) spectra in the same wavelength range for two fast-photocycling rhodopsins: green light-absorbing proteorhodopsin (GPR) and Gloeobacter rhodopsin (GR). In addition the frequency sweep shift can be detected completely even for tiny amounts of sample (10(-3) OD of rhodopsin). Based on these results NFMM shows both very high sensitivity for detecting conformational changes and produces a good time-resolved spectrum.</P>