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고광표 ( Gwangpyo Ko ),김진경 ( Jin-kyeong Kim ),조승화 ( Seong-wha Jo ),정도연 ( Do-youn Jeong ),운노타쯔야 ( Tatsuya Unno ) 한국응용생명화학회(구 한국농화학회) 2020 Journal of Applied Biological Chemistry (J. Appl. Vol.63 No.1
발효식품은 장 건강을 포함하여 건강상의 이로운점을 제공하는 건강기능식품으로 인식되고 있다. 따라서 본 연구는 Lactobacillus plantarum과 Bacillus amyloliquefaciens로 발효 된 커피원두가 건강한 사람의 장내미생물 생태에 미치는 영향을 조사하였다. 커피원두를 발효하여 플라보노이드와 폴리페놀과 같은 이로운 물질이 증가하였다. 또한 발효커피의 섭취로 인해 유의한 장내 미생물생태 및 물질대사 변화가 관찰되지 않았지만, 섬유소 분해 및 단쇄지방산을 생성하는 유익한 미생물이 증가하였다. 본 연구 결과는 발효커피 섭취로인해 장내미생물생태 및 물질대사를 유지하면서 유익한 미생물이 증가하였음을 확인하였다. Fermented foods have been recognized as functional foods that provide health benefits, including the modulation of intestinal microbiota. Therefore, the aim of the present study was to examine the effects of coffee beans fermented with Lactobacillus plantarum and Bacillus amyloliquefaciens on healthy human gut microbiota. Fermentation increased the content of beneficial substances (i.e., flavonoids and polyphenols). The consumption of fermented coffee increased the occurrence of beneficial microorganisms such as fiber degraders and short-chain fatty acid producers, although no significant microbiota shifts were observed after the coffee consumption. The analysis of metabolic activities also showed no difference after the coffee consumption. Our study demonstrates that the consumption of the fermented coffee may increase some beneficial bacterial while remaining the gut microbiota and its activities.
학교식당 및 교실배식 과정 전·후 미생물 오염에 관한 연구
정해용,손주혜,이재윤,이인애,고지연,고나윤,박성준,고광표,김성균,Jung, HeaYong,Sohn, JuHae,Lee, JaeYoon,Lee, InAe,Ko, JiYean,Ko, NaYun,Park, SungJun,Ko, GwangPyo,Kim, Sungkyoon 한국환경보건학회 2015 한국환경보건학회지 Vol.41 No.6
Objectives: The aim of this study is to investigate microbial contamination in the school food service environment for the assessment of microbial food safety. Methods: We collected both swab samples from tables and desks and airborne bacterial samples from an elementary school (School A) and a high school (School B). Heterotrophic plate count, total coliform, Staphylococcus aureus, and Bacillus cereus were measured with selective media to quantify microbial concentration. PCR assay targeting 16S rRNA genes was performed to identify the strains of S. aureus and B. cereus isolated. In addition, we made a food service checklist for the locations to evaluate the food service environment. A Wilcoxon test was employed to examine the differences in microbial concentration between before lunchtime and afterwards. Results: Heterotrophic plate counts showed higher levels after-lunch compared to before-lunch at School B. However, levels of S. aureus were higher in the after-lunch period (p<0.05) in both classrooms and in the cafeteria in School A. B. cereus was only sparsely detected in School B. Several samples from food dining carts were found to be contaminated with bacteria, and facilities associated with food delivery were found to be vulnerable to bacterial contamination. Although microbial concentrations in the air showed little difference between before- and after-lunchtime in the cafeteria in School A, those in classrooms were greater after-lunchtime at both schools. Conclusion: Our results suggested that the microbial safety in schools after lunchtime of concern. Necessary preventive measures such as hygiene education for students and food handlers should be required to minimize microbial contamination during food service processes in schools.
Park, SungJun,Ko, Young-Seon,Jung, Haeyong,Lee, Cheonghoon,Woo, Kyoungja,Ko, GwangPyo Elsevier 2018 The Science of the total environment Vol.625 No.-
<P><B>Abstract</B></P> <P>Silver nanoparticles (AgNPs) have been reported as an effective alternative for controlling a broad-spectrum of pathogenic viruses. We developed a micrometer-sized silica hybrid composite decorated with AgNPs (AgNP-SiO<SUB>2</SUB>) to prevent the inherent aggregation of AgNPs, and facilitated their recovery from environmental media after use. The production process had a high-yield, and fabrication was cost-effective. We evaluated the antiviral capabilities of Ag30-SiO<SUB>2</SUB> particles against two model viruses, bacteriophage MS2 and murine norovirus (MNV), in four different types of water (deionized, tap, surface, and ground). MNV was more susceptible to Ag30-SiO<SUB>2</SUB> particles in all four types of water compared to MS2. Furthermore, several water-related factors, including temperature and organic matter content, were shown to affect the antimicrobial capabilities of Ag30-SiO<SUB>2</SUB> particles. The modified Hom model was the best-fit disinfection model for MNV disinfection in the different types of water. Additionally, this study demonstrated that the effects of a certain level of physical obstacles in water were negligible in regards to the use of Ag30-SiO<SUB>2</SUB> particles. Thus, effective use of AgNPs in water disinfection processes can be achieved using our novel hybrid composites to inactivate various waterborne viruses.</P> <P><B>Highlights</B></P> <P> <UL> <LI> AgNP-SiO<SUB>2</SUB> can be synthesized using a high-yield, large-scale process. </LI> <LI> AgNP-SiO<SUB>2</SUB> maintained strong antiviral characteristics in different types of water. </LI> <LI> Modified Hom was the best model for murine norovirus disinfection using AgNP-SiO<SUB>2</SUB>. </LI> <LI> AgNP-SiO<SUB>2</SUB> can be used without significant risk to human health and the environment. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Effect of Metformin on Metabolic Improvement and Gut Microbiota
Lee, Heetae,Ko, GwangPyo American Society for Microbiology 2014 Applied and environmental microbiology Vol.80 No.19
<P>Metformin is commonly used as the first line of medication for the treatment of metabolic syndromes, such as obesity and type 2 diabetes (T2D). Recently, metformin-induced changes in the gut microbiota have been reported; however, the relationship between metformin treatment and the gut microbiota remains unclear. In this study, the composition of the gut microbiota was investigated using a mouse model of high-fat-diet (HFD)-induced obesity with and without metformin treatment. As expected, metformin treatment improved markers of metabolic disorders, including serum glucose levels, body weight, and total cholesterol levels. Moreover, <I>Akkermansia muciniphila</I> (12.44% ± 5.26%) and <I>Clostridium cocleatum</I> (0.10% ± 0.09%) abundances increased significantly after metformin treatment of mice on the HFD. The relative abundance of <I>A. muciniphila</I> in the fecal microbiota was also found to increase in brain heart infusion (BHI) medium supplemented with metformin <I>in vitro</I>. In addition to the changes in the microbiota associated with metformin treatment, when other influences were controlled for, a total of 18 KEGG metabolic pathways (including those for sphingolipid and fatty acid metabolism) were significantly upregulated in the gut microbiota during metformin treatment of mice on an HFD. Our results demonstrate that the gut microbiota and their metabolic pathways are influenced by metformin treatment.</P>