Streptomyces Genome Engineering for Biosynthesis and Regulation of a Novel Immunosuppressant Linear Polyketide, Tautomycetin

Eung?Soo Kim 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

통조림용 가다랑어육의 식품성분

오광수,김정균,김인수,이응호,김복규 國立統營水産專門大學 附設 水産科學硏究所 1990 수산과학연구소보고 Vol.2 No.-

가다랑어 혈합육을 보다 효율적으로 이용하기 위한 일련의 기초연구로서, 가다랑어 혈합육의 지질성분, 함질소엑스분 및 구성아미노산 등을 보통 육과 함께 분석하여 비교, 검토하였다. 보통육은 혈합육에 비해 수분, 조단백질 함량은 약간 많은 반면, 조지방과 회분 함량은 혈합육이 약간 많았다. 휘발성염기질소량은 보토육이 22.7mg/100g, 혈합육이 46.9mg/100ㅎ으로 혈합육이 보통육에 비해 선도저하가 훨씬 빨랐다. 보통육과 혈합육의 총지질 조성은 각각 중성지질 79.7%, 71.9%, 당지질, 6.8%, 9.5%, 인지질13.5%, 18.6%로 이루어져 있었다. 중성지질의 성분은 free fatty acid ac triglyceride, 인지질은 phosphatidyl ethanolamine, phosphatidyl, 인지질은 phosphatidyl choline이 주성분이었고, lysogud 인지질의축적도 확인되었다. 총지지르 중성, 당 및 인지질의 축적도 확인되었다. 종지질, 중성, 당 및 인지질의 지반산 조성에서 보통육은 16:0 및 18:1을 주체로 한 포화산 및 모노엔산의 조성비가 혈합육보다 높았으며, 혈합육은 폴리엔산, 특히 22:6의 비율이 월등히 높았다. 16:0. 18:0, 18:1, 20:5 및 22:6 등이 주요 구성지방산이었다. 유리아미노산의 조성은 총함량에서 보통육 쪽이 많았고, 보통육에는 histidine, 혈합육에는 tauring의 보통육에서는 680.9mg.100g 정도 전존해 있었으나, 혈합육에서는 IMP의 분해가 급속히 진행되어 73.1mg/100g에 지나지 않았다. TMAO 및 TMA는 보통육에 비해 혈합육에 휠씬 많이 함유되어 있었고, total creatinine은 보통육 쪽이 많았다. 구성아미노산의 조성은 양시료 간에 별차이가 없었고, glutamic acid, aspartic acid, lysine, leucine, alanine, isoleucine 및 arginine 등이 주요 구성아니노산이었다. The lipid components, nitrogenous extracts and amino acids of dark muscle(DM) of skipjack (Katsuwonus pelamis) were analyzed and compared with those of white muscle(WM). WM was higher in moisture and crude protein content, and lower in crude lipid and ash content than those of DM. Contents of volatile basic nitrogen in WM and DM were 22.7mg/100g and 46.9mg/100g. Total lipid(TL) of WM and DM consisted of 79.7%, 71.9% neutral lipid(NL), 6.8%, 9.5% glycolipid(GL), and 13.5%, 18.6% phospholipid(PL), respectively. NL was mainly composed of free fatty acid, triglyceride, and PL was mainly occupied by phosphatidyl ethanolamine, phosphatidyl choline. Also lysophosphatidyl choline and lysophosphatidyl ethanolamine were identified in PL. In fatty acid composition of TL, NL, GL and PL, WM revealed higher contents in saturates and monoenes such as 16 : 0, 18 : 1, while DM showed higher contents in polyenes such as 22 : 6 especially. The major fatty acids of these samples were generally 16 : 0, 18 : 0, 18 : 1, 20 : 5 and 22 : 6. Contents of total free amino acids from WM and DM were 5,982.3mg/100g and 4,450.7mg/100g (dry base). Of free amino acids, Tau concentration was much higher in DM than in WM, Ala, Gly, Met, Arg, Thr were also high in DM. But His was much higher in concentration in WM. Content of inosinic acid(IMP) in WM(680.9mg/100g) was higher than that of DM(73.1mg/100g). The degradations of IMP proceeded very rapidly in DM. DM contained much higher trimethylamine oxide and trimethylamine than those of WM. The profile of combined amino acids in these samples were very similar, and main amino acids were Glu, Asp, Lys, Ala, Ile and Arg.

Gate 전극의 post annel에 따른 GOI의 특성

김응수 부산 외국어 대학교 2000 外大論叢 Vol.20 No.1

The characteristics of GOI (Gate Oxide Integrity) according to post gate anneal were investigated, and it is found that the property of polycrystalline silicon gate electrode and reliability of then gate oxide are strongly affected by the post anneal. The strong compressive stress in amorphous silicon gate is relaxed as the micro-structure of the gate changes to polycrystalline silicon by the increase of anneal temperature. The reliability of then gate oxide and interface properties between oxide and silicon substrate greatly depends on the activation anneal temperature. Particularly, the interface trap density at oxide/silicon decreases with the increasing of anneal temperature, which is consistent with the behavior of polycrystalline silicon gate stress.

폐선암조직에서 Neutral Ribonuclease의 분리와 성상에 관한 연구

김응수,고재경,지행옥 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

Concentrations of nucleic acids and proteins were determined in adenocarcinoma tissue of the lung and were compared with those in the control lung tissue. Also studied were properties of the neutral RNase specific to the lung cancer to investigate the possible role of the RNase in process involved in carcinogenesis of adenocarcinoma of the lung. DNA and protein centents were unchanged, but RNA content was increased in adenocarcinoma tissue of the lung. Neutral RNase activity was unchanged in the cancer tissue, indicating that the RNase could not be used as a marker for the lung cancer. Proteins and neutral RNase in adenocarcinoma tissue of the lung were separated by a DEAE-cellulose column chromatography into 7peaks each, of which the peak I neutral RNase isozyme was not found in the control lung tissue. This indicated that the peak I neutral RNase was specific to the adenocarcinoma of the lung. The peak I neutral RNase isolated from the adenocarcinoma tissue of the lung did not hydrolyze single stranded (ss) polydeoxyribonucleotides and double stranded (ds) polynucleotides, but hydrolyzed ss polyribonucleotides. The enzyme was observed to be highly active toward poly C, poly U and RNA, indicating that the RNase appeared to be mixed type of secretory and nonseretory RNase. The peak I RNase was not active toward A-A and G-G linkages, but unusually highly active toward A-C and A-U linkages (4 to 6 fold as active as C-C linkage). These results indicated that the peak I neutral RNase isolated from the adenocarcinoma of the lung was (1)specific to the lung cancer. (2) mixed type of seretory and nonsecretory enzymes, (3) unusually highly active toward A-C and A-U linkages of ss polyribonucleotides and RNA, suggesting that the RNase might play roles in processes involved in carcinogenesis and suppression of cancer.

MOCVD법에 의해 제조된 Fe₂O₃-TiO₂계 박막의 내식성과 광전류 응답 특성에 관한 연구

金顯洙,尹在弘,金明鎬,杉本克久,邊鷹善 國立 昌原大學校 産業技術硏究所 1998 産技硏論文集 Vol.12 No.-

Fe₂O₃-TiO₂ films were formed by MOCVD technique, and their corrosion resistance and photoelectrochemical property were examined in acidic, neutral and alkaline solutions. The corrosion resistance was examined by an anodic polarization test, and the photocurrent response by a photoelectrochemical polarization test. The photocurrent and quantum yield maximum of the films depend on the titanium cationic fraction(Xη) in the films. They decreased in the range of Xη=0.0-0.70, then increased in the range of Xη=0.70-0.95, and finally decreased in the range of Xη=0.91-1.0 with increasing Xηvalue. The increase in the photocurrent and quuantum yield maximum in the range of Xη=0.70-0.95 was attributed to the amorphous structure. Such a change in the photocurrent and quantum yield maximum was independent to the solution pH. The corrosion resistance of the films increased with increasing Xηvalue of the film in acidic, neutral and alkaline solutions. Therefore, the films with Xη=0370-0.95 can be concluded to have high photocurrent response and high corrosion resistance.

대구광역시 중구의 가로수 및 열섬 모자이크 현황 분석

김수봉,정응호,김기호 한국환경과학회 2006 한국환경과학회지 Vol.15 No.4

대장암조직에서 아포토시스 검출 방법에 관한 연구

김광호,심강섭,김성숙,구혜수,박응범 梨花女子大學校 醫科大學 醫科學硏究所 1997 EMJ (Ewha medical journal) Vol.20 No.2

1989년 1월부터 1992년 12월 사이에 대장암으로 근치적 절제술을 시행받은후 추적조사가 가능한 45명의 환자를 대상 대장암조직의 Hematoxylin-Eosin염색을 통하여 apoptotic body를 광학 현미경하에서 400배율 하에서 관찰하여 암세포 100개당 평균개수를 세었으며 TUNEL 방법을 이용하여 면화학적염색을 통하여 염색정도를 판정을하여 대장암에서 아포토시스의 정도를 판정하여 다음과 같은 결과를 얻었다. Dukes병기 A 일 때 apoptotic body가 평균 1.93개, B 일 때 2.66개, C 인 경우 2.76개 였으며 분화도에 따라서는 고분화암인 경우 2.51개, 중등도분화암인 경우는 3.01개, 저분화암인 경우는 2.15개였다. 5년내 사망한 환자에서는 3.55개 였으며 5년이상 생존한 환자에서는 2.8개였다. 형태학적으로 apoptotic body의 수를 세어 아포토시스를 측정하여 예후예측인자로 사용할 수 있으리라 사료되며 아포토시스와 p53의 발현과의 관계를 보았을 때 특별한 상관관계를 발견 할 수 없어 bcl-2의 발현과 더불어 아토토시스와의 관게에 대하여 좀더 연구가 되어야 하겠다. Objectives : Apoptosis is a specific mode of cell death recognized by a characteristic pattern of morphological, biochemical, and molecular changes, There are several methods of detection of apoptosis. Morphological changes involve a characteristic pattern of chromation and cytoplasm. The landmark of apoptosis is endonucleolysis, with nuclear DNA initially degraded at the linker sections to fragments equivalent to single and multiple nucleosomes. Detection of DNA fragments is situ using the terminal deoxyribonucleotidyl transferase(TDT)-mediated dUTP-digoxigenin nick end labeling (TUNEL) assay is increasingly applied to investigate apoptosis. We studied the detection method of apoptosis morphologically and by using TUNEL assay and examined the correlation of p53 expression and apoptosis. Methods : Forty-five cases of colorectal cancer were selected. The number of apoptotic bodies was expressed as a number per 100 cancer cells. The TUNEL assay was performed with in situ Apoptag kit®. Results : The mean number of the apoptotic bodies was 2.28 in the patients who survived over 5 years after curative resection and 3.55 in the patients who died within 5 years(p=0.001). There was a relationship between the number of apoptotic bodies which were measured by morphologic study and the results which were measured by TUNEL assay. There was no relationship between p53 expression and apoptosis. Conclusion : These results suggest that the frequency of apoptotic bodies may be a prognostic factor for colorectal cancer and apoptosis could be measured by morphological study without special study.

센서를 이용한 소형의 보안 시스템 설계 및 제작

김응수,손기환 부산 외국어 대학교 2004 外大論叢 Vol.28 No.-

A security system using an infrared sensor was fabricated to prevent an intruder. The fabricated system has pasword key pad to permit someone to enter the house and office. The control circuit of the system is designed by VHDL(Very high speed integrated Hardware Description Language). The output signals were displayed in LCD, LED, buzzer. This designed system in this paper has a advantage to supplement additional function with ease.

광섬유와 도파로를 이용한 압력센서

김응수,강신원 부산 외국어 대학교 2003 外大論叢 Vol.26 No.-

Novel pressure sensor consisted of single mode fiber and planar waveguide was demonstrated. The resonance wavelength of sensor was shifted due to refractive index variation of polymers by pressure and its sensitivity depended on materials of waveguide. We adopted symmetric waveguide structure to solve the problem of polarization dependence in side polished fiber. AZ4562 and THB30 are used as a waveguide materials and the sensitivity was -0.008㎚/bar and 0.16㎚/bar, respectively.

중복 자궁 경부, 맹낭성 종질중격 및 동측 신장발생부전을 가진 교통성 중복자궁 1례

김윤진,전명권,이응수,최형민 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

The class of uterine malformation known as communicating uteri is characterized by two uterocervical cavities connected by an isthmic communication. We experienced case of communicating double uterus with double cervix, blind hemivagina and ipsilateral renal agenesis. The complaint of the patient was odorous vaginal discharge with lower abdominal pain unrelated with menstrual cycle. We confirmed these Mullerian anomalies by transvaginal ultrasound. IVP(intravenous pyelography), MRI(magnetic resonance imaging), hysterosalpingography and hysteroscopy. After vaginal septoplasty, her complaint was subsided. We report this case with brief review of literatures.