Profiling of remote skeletal muscle gene changes resulting from stimulation of atopic dermatitis disease in NC/Nga mouse model

Donghee Lee,Yelim Seo,Young-Won Kim,Seongtae Kim,Jeongyoon Choi,Sung-Hee Moon,Hyemi Bae,Hui-sok Kim,Hangyeol Kim,Jae-Hyun Kim,Tae-Young Kim,Eunho Kim,Suemin Yim,Inja Lim,Hyoweon Bang,Jung-Ha Kim,Jae-H 대한약리학회 2019 The Korean Journal of Physiology & Pharmacology Vol.23 No.5

Although atopic dermatitis (AD) is known to be a representative skin disorder, it also affects the systemic immune response. In a recent study, myoblasts were shown to be involved in the immune regulation, but the roles of muscle cells in AD are poorly understood. We aimed to identify the relationship between mitochondria and atopy by genome-wide analysis of skeletal muscles in mice. We induced AD-like symptoms using house dust mite (HDM) extract in NC/Nga mice. The transcriptional profiles of the untreated group and HDM-induced AD-like group were analyzed and compared using microarray, differentially expressed gene and functional pathway analyses, and protein interaction network construction. Our microarray analysis demonstrated that immune response-, calcium handling-, and mitochondrial metabolism-related genes were differentially expressed. In the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology pathway analyses, immune response pathways involved in cytokine interaction, nuclear factor-kappa B, and T-cell receptor signaling, calcium handling pathways, and mitochondria metabolism pathways involved in the citrate cycle were significantly upregulated. In protein interaction network analysis, chemokine family-, muscle contraction process-, and immune response-related genes were identified as hub genes with many interactions. In addition, mitochondrial pathways involved in calcium signaling, cardiac muscle contraction, tricarboxylic acid cycle, oxidation-reduction process, and calcium-mediated signaling were significantly stimulated in KEGG and Gene Ontology analyses. Our results provide a comprehensive understanding of the genome-wide transcriptional changes of HDM-induced AD-like symptoms and the indicated genes that could be used as AD clinical biomarkers.

Long-term clinical course and outcome of Korean patients with microscopic polyangiitis associated interstitial lung disease

( Donghee Lee ),( Jinwoo Song ),( Jooae Choe ),( Heesang Hwang ) 대한결핵 및 호흡기학회 2019 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.127 No.-

Background: Microscopic polyangiitis (MPA) is a systemic vasculitis affecting small vessels. Interstitial lung disease (ILD) is the most common pulmonary manifestations of MPA and is known to be associated with poor outcome. This study aimed to investigate clinical course and outcome in Korean patients with MPA -ILD and to find out prognostic factors. Methods: Clinical data were retrospectively analyzed in 37 patients with MPA-ILD (biopsy proven case, n=5) between 2000 to 2019 at Asan Medical Center. High resolution computed tomography (HRCT) images were categorized as usual interstitial pneumonia (UIP) pattern and others. Results: The median follow-up period was 51.2 months. Of total patients, the mean age was 68.8 years, 62% was male, and 64% was ever-smokers. Six (16.2%) patients died during followup, and the median survival period was 51.5 months (survival rate, 1 year: 86.5%, 3 year: 64.9%, 5 year 46%). Nonsurvivors, at baseline, showed lesser frequent renal involvement and a tendency to have older age, and lesser frequent MPO-positivity, than survivors. However, there were no differences in gender, respiratory symptoms, lung functions, exercise capacity and bronchoalveolar lavage fluid findings between nonsurvivors and survivors. During follow-up, there were more frequent acute exacerbation, pneumonia and alveolar hemorrhage in nonsurvivors compared with survivors. In multivariate Cox analysis, old age was an independent prognostic factor (hazard ratio: 1.209, 95% confidence interval: 1.003-1.457, p=0.046 ) in patients with MPA-ILD. Conclusions: In this study, clinical course and outcome of Korean patients with MPA-ILD were similar to previous reports. Old age means poor prognosis in patients with MAP-ILD.

Selective SnO_<i>x</i> Atomic Layer Deposition Driven by Oxygen Reactants

Lee, Jung-Hoon,Yoo, Mi,Kang, DongHee,Lee, Hyun-Mo,Choi, Wan-ho,Park, Jung Woo,Yi, Yeonjin,Kim, Hyun You,Park, Jin-Seong American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.39

<P>SnO<SUB><I>x</I></SUB> thin films were successfully deposited by the thermal atomic layer deposition (ALD) method using <I>N</I>,<I>N</I>′-<I>tert</I>-butyl-1,1-dimethylethylenediamine stannylene(II) as a precursor and ozone and water as reactants. The growth of SnO and SnO<SUB>2</SUB> films could be easily controlled by employing different reactants and utilizing different ozone and water concentrations, respectively. The formation of both SnO and SnO<SUB>2</SUB> films exhibited typical surface-limiting reaction characteristics, although their growth behaviors differ from one another. The combined studies of density functional theory calculations and experimental analyses showed that the difference in growth behavior of the SnO and SnO<SUB>2</SUB> films can be attributed to the stability of ozone and water on the SnO<SUB>2</SUB> and SnO films. SnO and SnO<SUB>2</SUB> films have different crystal structures and both films were crystallized from the amorphous to polycrystalline states following an increase in the deposition temperature. The absorbance and refractive index of the thin films were investigated using ultraviolet-visible spectroscopy (UV-vis) and spectroscopic ellipsometry (SE), respectively. SnO<SUB><I>x</I></SUB> films formed using ozone and water as a reactant showed an optical band gap of 3.60-3.17 eV and 2.24-2.30 eV and refractive indices of ∼2.0 and ∼2.6, respectively, which correspond to values typical of SnO<SUB>2</SUB> and SnO. The bilayer structure of SnO/SnO<SUB>2</SUB> was successfully fabricated on indium tin oxide (ITO) glass with nickel as a top electrode at 100 °C. The SnO/SnO<SUB>2</SUB> bilayer exhibited diode characteristics with a current rectification ratio of 15. Our results present a simple but highly versatile growth method for producing multilayer oxide films with electronic properties that can be finely controlled.</P> [FIG OMISSION]</BR>

Risk factors for lesions of the knee menisci among workers in South Korea’s national parks

Donghee Shin,Kanwoo Youn,Eunja Lee,Myeongjun Lee,Hweemin Chung,Deokweon Kim 대한직업환경의학회 2016 대한직업환경의학회지 Vol.28 No.-

Background: This study was designed to investigate the prevalence of the menisci lesions in national park workers and work factors affecting this prevalence. Methods: The study subjects were 698 workers who worked in 20 Korean national parks in 2014. An orthopedist visited each national park and performed physical examinations. Knee MRI was performed if the McMurray test or Apley test was positive and there was a complaint of pain in knee area. An orthopedist and a radiologist respectively read these images of the menisci using a grading system based on the MRI signals. To calculate the cumulative intensity of trekking of the workers, the mean trail distance, the difficulty of the trail, the tenure at each national parks, and the number of treks per month for each worker from the start of work until the present were investigated. Chi-square tests was performed to see if there were differences in the menisci lesions grade according to the variables. The variables used in the Chi-square test were evaluated using simple logistic regression analysis to get crude odds ratios, and adjusted odds ratios and 95 % confidence intervals were calculated using multivariate logistic regression analysis after establishing three different models according to the adjusted variables. Results: According to the MRI signal grades of menisci, 29 % were grade 0, 11.3 % were grade 1, 46.0 % were grade 2, and 13.7 % were grade 3. The differences in the MRI signal grades of menisci according to age and the intensity of trekking as calculated by the three different methods were statistically significant. Multiple logistic regression analysis was performed for three models. In model 1, there was no statistically significant factor affecting the menisci lesions. In model 2, among the factors affecting the menisci lesions, the OR of a high cumulative intensity of trekking was 4.08 (95 % CI 1.00–16.61), and in model 3, the OR of a high cumulative intensity of trekking was 5.84 (95 % CI 1.09–31.26). Conclusion: The factor that most affected the menisci lesions among the workers in Korean national park was a high cumulative intensity of trekking.

A Study on the Development of Interview Procedure for HSA at Kori Unit 1

Donghee Lee,Wook Sohn,Sukbon Yoon,Suhee Lee 한국방사성폐기물학회 2018 한국방사성폐기물학회 학술논문요약집 Vol.2018 No.05

Comparative Assessment of Radiation Shielding in Low Water Level Spent Fuel Pools

Donghee Lee,Yongdeog Kim,Kiyoung Kim 한국방사성폐기물학회 2023 한국방사성폐기물학회 학술논문요약집 Vol.21 No.2

After the Fukushima disaster, overseas nuclear power plants have established conditions for issuing a red alert in the event of fuel damage within the spent fuel pool and they have already implemented conditions for issuing a blue alert when fuel is exposed above the water surface. In South Korean nuclear power plants, a real-time monitoring system is in place to oversee the exposure of spent fuel to the surface within the spent fuel pool. To achieve this, a water level indicator gauge is installed within the spent fuel pool, allowing for continuous real-time monitoring. This paper conducted a comparative assessment of radiation levels from water level monitoring system in two units’ spent fuel pools based on the low water levels (1 feet from the storage rack), utilizing the radiation analysis code (MCNP).

Management and Disposal Plans for Spent Fuel/HLW in Various Countries Around the World

Donghee Lee,Taehyeon Kim,TaeHyung Na 한국방사성폐기물학회 2023 한국방사성폐기물학회 학술논문요약집 Vol.21 No.1

As of 2023, there has been significant progress worldwide in the management of nuclear fuel’s spent radioactive waste (HLW). Several countries have made important strides in advancing their plans for the construction of deep geologic repositories (DGRs) to safely dispose of their nuclear waste. Finland led the way, with its nuclear waste management organization, Posiva Oy, submitting an application for an operating license for a DGR for spent fuel generated by the nuclear power plants of its owners. The facility, ONKALO, will be located on the island of Olkiluoto and is expected to begin final disposal in the mid-2020s. Sweden also approved SKB’s application to build a DGR in Forsmark, and an encapsulation plant next to the Clab interim storage facility. In Switzerland, Nagra selected Nordic Lagern as the site for the Swiss DGR, and is preparing the general license applications for the required facilities. Meanwhile, Canada’s Nuclear Waste Management Organization (NWMO) narrowed down the possible locations for its DGR to two, and expects to name its preferred site by fall 2024. The UK established four Community Partnerships to participate in the siting process for a DGR, with Nuclear Waste Services (NWS) responsible for identifying a site. Andra, the French organization responsible for managing all French radioactive waste, is expected to submit an application by the end of the year for a DGR in France that will contain HLW resulting from reprocessing of spent fuel assemblies from French nuclear power plants, as well as intermediate-level waste. Overall, the progress made by these countries represents a tangible and sustainable step forward in the management of spent fuel and HLW, and brings us closer to the safe and effective long-term disposal of nuclear waste.

Magnetic pumping of continuous magnetic fluid in a spin-valve sensor

Lee, Kisu,Park, Jeong-Ho,Lee, Donghee,Lee, Sangyoung,Yang, S.,Cho, B. K. American Institute of Physics 2011 JOURNAL OF APPLIED PHYSICS - Vol.109 No.7

Comparative Analysis of Burnup Uncertainty for Spent Fuel

Donghee Lee,Yongdeog Kim,Kiyoung Kim 한국방사성폐기물학회 2023 한국방사성폐기물학회 학술논문요약집 Vol.21 No.1

After spent fuel is stored in a dry storage container, it becomes difficult to obtain information on the fuel’s characteristics. As a result, it is necessary to identify the characteristics of spent nuclear fuel in advance and secure the information necessary to establish delivery acceptance requirements for interim storage and disposal in the future. Therefore, it is necessary to evaluate the characteristics of spent fuel before loading dry storage casks. In order to prepare for the dry storage of spent fuel, information on the basic characteristics of the fuel is required. As part of this information, it is also necessary to establish calculation criteria for spent fuel burnup. Spent fuel burnup can be classified into three categories. The first is burnup evaluated using design codes (design burnup), the second is burnup measured by furnace instruments during power plant operation (actual burnup), and the third is burnup measured through measurement equipment (measured burnup). This paper describes a comparative evaluation of design burnup, actual burnup, and measured burnup for specific fuels (40 bundles).

Improvement Characteristics of Bio-active Materials Coated Fabric on Rat Muscular Mitochondria

Lee, Donghee,Kim, Young-Won,Kim, Jung-Ha,Yang, Misuk,Bae, Hyemi,Lim, Inja,Bang, Hyoweon,Go, Kyung-Chan,Yang, Gwang-Wung,Rho, Yong-Hwan,Park, Hyo-Suk,Park, Eun-Ho,Ko, Jae-Hong The Korean Society of Pharmacology 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.3

This study surveys the improvement characteristics in old-aged muscular mitochondria by bio-active materials coated fabric (BMCF). To observe the effects, the fabric (10 and 30%) was worn to old-aged rat then the oxygen consumption efficiency and copy numbers of mitochondria, and mRNA expression of apoptosis- and mitophagy-related genes were verified. By wearing the BMCF, the oxidative respiration significantly increased when using the 30% materials coated fabric. The mitochondrial DNA copy number significantly decreased and subsequently recovered in a dose-dependent manner. The respiratory control ratio to mitochondrial DNA copy number showed a dose-dependent increment. As times passed, Bax, caspase 9, PGC-$1{\alpha}$ and ${\beta}$-actin increased, and Bcl-2 decreased in a dose-dependent manner. However, the BMCF can be seen to have had no effect on Fas receptor. PINK1 expression did not change considerably and was inclined to decrease in control group, but the expression was down-regulated then subsequently increased with the use of the BMCF in a dose-dependent manner. Caspase 3 increased and subsequently decreased in a dose-dependent manner. These results suggest that the BMCF invigorates mitophagy and improves mitochondrial oxidative respiration in skeletal muscle, and in early stage of apoptosis induced by the BMCF is not related to extrinsic death-receptor mediated but mitochondria-mediated signaling pathway.