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( Do Yup Lee ),( Oliver Fiehn ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.7
Rapamycin, known as an inhibitor of Target of Rapamycin (TOR), is an immunosuppressant drug used to prevent rejection in organ transplantation. Despite the close association of the TOR signaling cascade with various scopes of metabolism, it has not yet been thoroughly investigated at the metabolome level. In our current study, we applied mass spectrometric analysis for profiling primary metabolism in order to capture the responsive dynamics of the Chlamydomonas metabolome to the inhibition of TOR by rapamycin. Accordingly, we identified the impact of the rapamycin treatment at the level of metabolomic phenotypes that were clearly distinguished by multivariate statistical analysis. Pathway analysis pinpointed that inactivation of the TCA cycle was accompanied by the inhibition of cellular growth. Relative to the constant suppression of the TCA cycle, most amino acids were significantly increased in a time-dependent manner by longer exposure to rapamycin treatment, after an initial down-regulation at the early stage of exposure. Finally, we explored the isolation of the responsive metabolic factors into the rapamycin treatment and the culture duration, respectively.
TSC2 genetic variant and prognosis in non‐small cell lung cancer after curative surgery
Lee, Yong Hoon,Do, Sook Kyung,Lee, Shin Yup,Kang, Hyo‐,Gyoung,Choi, Jin Eun,Hong, Mi Jeong,Lee, Jang Hyuck,Lee, Eung Bae,Jeong, Ji Yun,Shin, Kyung Min,Lee, Won Kee,Seok, Yangki,Cho, Sukki,Yoo, S Wiley-Blackwells 2019 Thoracic Cancer Vol.10 No.2
<P>This study was conducted to investigate the associations between polymorphisms of genes involved in the LKB1 pathway and the prognosis of patients with non‐small cell lung cancer (NSCLC) after surgical resection. Twenty‐three single nucleotide polymorphisms (SNPs) in the LKB1 pathway were investigated in 782 patients with NSCLC who underwent curative surgery. The association of SNPs with overall survival (OS) and disease‐free survival (DFS) were analyzed. Among the 23 SNPs investigated, <I>TSC2</I> rs30259G > A was associated with significantly worse OS and DFS (adjusted hazard ratio for OS 1.88, 95% confidence interval 1.21–2.91, <I>P</I> = 0.005; adjusted hazard ratio for DFS 1.65, 95% confidence interval 1.15–2.38, <I>P</I> = 0.01, under codominant models, respectively). Subgroup analysis showed that SNPs were significantly associated with survival outcomes in squamous cell carcinoma, ever‐smokers, and stage I, but not in adenocarcinoma, never‐smokers, and stage II–IIIA. The results suggest that <I>TSC2</I> rs30259G > A may be useful to predict prognosis in patients with NSCLC, especially squamous cell carcinoma, after curative surgery.</P>
Lipidomic profiles disturbed by the internet gaming disorder in young Korean males
Lee, Chang-Wan,Lee, Deokjong,Lee, Eun Mi,Park, Soo Jin,Ji, Dong Yoon,Lee, Do Yup,Jung, Young-Chul Elsevier 2019 Journal of chromatography. B, Analytical technolog Vol.1114 No.-
<P><B>Abstract</B></P> <P>Internet Gaming Disorder (IGD) is characterized by uncontrollable and persistent playing of internet games despite the occurrence of negative consequences. Although there is a worldwide treatment demand, IGD still doesn't have an explicit biomarker.</P> <P>The primary goal of the study is to characterize lipidomic profiles specific to internet gaming disorder (IGD) based on liquid-chromatography Orbitrap mass-spectrometry (LC Orbitrap MS). Primarily, a total of 19 lipids were significantly dys-regulated in the IGD group compared to healthy controls. The lipidomic feature was mainly characterized by various types of phosphatidylcholines (PCs) and lyso-phosphatidylcholines (LysoPCs). Subsequent multivariate statistical model and linear regression model prioritized two LysoPCs (C16:0 and C18:0) for potential biomarker. Receiver operating characteristic (ROC) analysis demonstrated excellent performance of the combined lipid set for discriminating the IGD group from healthy controls (AUC: 0.981, 95% confidence interval: 0.958–1.000). Additional evaluation with potential confounders and clinical parameters suggested robustness and potential applicability of the outcome as biomarkers which may aid diagnosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lipid profiles revealed lipid metabolism dys-regulation by internet gaming disorder. </LI> <LI> Discovery of biomarker cluster in combination with LysoPC 16:0 and LysoPC 18:0 </LI> <LI> Validation of biomarker recomposite by evaluation of potential confounding effects. </LI> </UL> </P>
Do, Sook Kyung,Choi, Sun Ha,Lee, Shin Yup,Choi, Jin Eun,Hong, Mi Jeong,Kang, Hyo-Gyoung,Lee, Won Kee,Lee, Eung Bae,Shin, Kyung Min,Jeong, Ji Yun,Lee, Yong Hoon,Seo, Hyewon,Yoo, Seung Soo,Lee, Jaehee,C Elsevier 2019 Gene Vol.703 No.-
<P><B>Abstract</B></P> <P>This study was conducted to explore whether polymorphisms of glucose transporter 3 (<I>GLUT3</I>) gene affect the prognosis of patients with non-small cell lung cancer (NSCLC) after surgical resection. Four single nucleotide polymorphisms (SNPs) in <I>GLUT3</I> were investigated in a total of 782 patients with NSCLC who underwent curative surgery. The association of the SNPs with overall survival (OS) and disease free survival (DFS) was analyzed. Among the four SNPs investigated, <I>GLUT3</I> rs7309332C>T was significantly associated with OS and DFS in multivariate analyses. The SNP was associated with significantly worse OS (adjusted hazard ratio [aHR] = 1.62, 95% confidence interval [CI] = 1.04–2.53, <I>P</I> = 0.03, under recessive model), and worse DFS (aHR = 1.64, 95% CI = 1.18–2.29, <I>P</I> = 0.003, under recessive model). When stratified by tumor histology, the association between the <I>GLUT3</I> rs7309332C>T and OS/DFS was not limited to either squamous cell carcinoma (SCC) or adenocarcinoma (AC), although the significant association remained only in AC for OS (<I>P</I> = 0.40 for SCC and <I>P</I> = 0.04 for OS) and only in SCC for DFS (<I>P</I> = 0.03 for SCC and <I>P</I> = 0.08 for OS). When AC patients were stratified according to <I>EGFR</I> mutation status, the SNP was significantly associated with DFS in patients with <I>EGFR</I> mutant tumors (aHR = 2.47, 95% CI = 1.15–5.30, <I>P</I> = 0.02, under recessive model), but not in those with <I>EGFR</I> wild-type tumors. This study suggests that genetic variation in <I>GLUT3</I> may be useful in predicting survival of patients with early stage NSCLC.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>GLUT3</I> variant was significantly associated with survival of NSCLC after surgery. </LI> <LI> The association was not limited to either squamous cell carcinoma or adenocarcinoma. </LI> <LI> The SNP may help identify patients at high risk of poor outcome in early stage NSCLC. </LI> </UL> </P>
Lee, Kyoung G.,Wi, Rinbok,Imran, Muhammad,Park, Tae Jung,Lee, Jaebeom,Lee, Sang Yup,Kim, Do Hyun American Chemical Society 2010 ACS NANO Vol.4 No.7
<P>Silica nanorods were successfully prepared through a sol−gel process in the presence of carboxylic-functionalized single-walled carbon nanotubes (C-SWCNTs). The effect of chemical functionalization of single-walled carbon nanotubes (SWCNTs) on the growth of the silica layer was investigated using pristine SWCNTs (P-SWCNTs) and C-SWCNTS. The C-SWCNTs served as a unique template to fabricate silica hybrid composite materials. The crystalline formation and growing mechanism of the silica layer on C-SWCNTs were explained by the hydrolysis and chemical bonding between silica precursors and carboxylated SWCNTs. The C-SWCNTs, as templates, were successfully encapsulated using silica, and used templates were removed by oxidation at high temperature. Finally, silica nanorods/nanowires were synthesized in forms of mold, and this silica fabrication mechanism could be applied for large-scale production of silica nanomaterials and highly flexible nanocomposites. The sequence of a silica encapsulation process of C-SWCNTs and removed C-SWCNTs was characterized using SEM, TEM, EDX, FT-IR and Raman spectroscopy, XRD, and electrical analysis.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2010/ancac3.2010.4.issue-7/nn100807r/production/images/medium/nn-2010-00807r_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn100807r'>ACS Electronic Supporting Info</A></P>
<i>In Vitro</i> Biosynthesis of Metal Nanoparticles in Microdroplets
Lee, Kyoung G.,Hong, Jongin,Wang, Kye Won,Heo, Nam Su,Kim, Do Hyun,Lee, Sang Yup,Lee, Seok Jae,Park, Tae Jung American Chemical Society 2012 ACS NANO Vol.6 No.8
<P>We report the use of a hydrogel polymer, recombinant <I>Escherichia coli</I> cell extracts, and a microdroplet-based microfluidic device to fabricate artificial cellular bioreactors which act as reactors to synthesize diverse metal nanoparticles (NPs). The combination of cell extracts, microdroplet-based microfluidic device, and hydrogel was able to produce a mass amount of artificial cellular bioreactors with uniform size and shape. For the first time, we report the alternating generation of microdroplets through one orifice for the fabrication of the artificial cellular reactors using the cell extract as inner cellular components and hydrogel as an artificial cellular membrane. Notably, the hydrogels were able to protect the encapsulated cell extracts from the surrounding environment and maintain the functionality of cellular component for the further cellular bioreactor applications. Furthermore, the successful applications of the fabricated artificial cellular bioreactors to synthesize various NPs including quantum dots, iron, and gold was demonstrated. By employing this microfluidic technique, the artificial cellular bioreactors could be applicable for the synthesis of diverse metal NPs through simple dipping of the reactors to the metal precursor solutions. Thus, the different size of NPs can be synthesized through controlling the concentration of metal precursors. This artificial cellular bioreactors offer promising abilities to biofriendly ways to synthesis diverse NPs and can be applicable in chemical, biomedical, and bioengineering applications.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2012/ancac3.2012.6.issue-8/nn302043q/production/images/medium/nn-2012-02043q_0001.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn302043q'>ACS Electronic Supporting Info</A></P>