Clinical Significance of Serum Vascular Endothelial Growth Factor and Complement 3a Levels in Patients with Colorectal Cancer in Southern Iran

Mehrabani, Davood,Shamsdin, Seyedeh Azra,Dehghan, Alireza,Safarpour, Alireza Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: Colon cancer (CRC) is perhaps the second most common cause of cancer mortality. This study determined the clinical significance of serum vascular endothelial growth factor (VEGF) and serum complement 3a (C3a) levels in patients with CRC in Fars province, southern Iran. Materials and Methods: Between June 2010 and June 2012, 110 patients with CRC of both genders and different age groups were divided into 3 groups. Group A included patients who had just undergone surgery; Group B had undergone chemotherapy after surgery; and Group C had undergone chemotherapy and radiotherapy after surgery. Twenty one healthy subjects with normal colonoscopy were considered as a control group. ELISA was undertaken to determine VEGF and C3a levels before and after treatment measures. Results: The mean age of patients was $53.9{\pm}14.1$ years. Considering VEGF level, a significant decrease was visible after treatment measures in groups A and B, but not Group C. For VEGF level, the difference was not statistically significant between two genders and various age groups before and after treatment. No significant difference was found for VEGF level between patients and normal group before any treatment. Regarding C3a levels in 101 subjects, they significantly decreased after treatment measures. Before and after treatment, the difference was statistically significant between two genders, but was not statistically significant among various age groups. Conclusions: As VEGF and C3a levels were significantly lower in patients after treatment, these may be beneficial markers in assessment of CRC therapy especially in early stages.

Disorders of Liver : Prevalence of Hepatitis D In Patients With Chronic Active Hepatitis B, And Comparison of Liver Enzymes And Histopathology In The Positive And Negative Group

( Seyed Alineza Taghavi ),( Davood Mehrabani ),( Farnaz Khademolhosseini ) 대한소화기학회 2007 SIDDS Vol.9 No.-

Background/Aims: The term viral hepatitis is used to describe viral infections that primarily involve the liver. Six main viruses have been recognized, as agents responsible for viral hepatitis, including hepatitis A, B, C, D, E and G viruses. The aim of this study was to determine the prevalence and histopathological characteristics of hepatitis D in patients with chronic active hepatitis B. Methods: In this study, anti-HDV antibody was checked by ELISA (Enzyme linked immunosorbent assay) method in 93 patients with chronic active hepatitis B (HBsAg +ve with>1.5 times normal aminotransferases and active liver pathology). Patients were selected from referrals to Mothahari Clinic in Shiraz in two consecutive years (1381-1382). Results: Among 93 Patients, 9 (9.7%) were seropositive for anti HDV antibody. Overall, 76.3% of our patients were males, and the positive group were all males. Comparison of liver enzymes (AST and ALT) and liver histology (grade and stage of liver disease) in the positive and negative group revealed a significantly higher AST (P value: 0.016) in the positive group. Mean ALT levels were also higher in positive group, but the difference was not statistically significant (P value: 0.162). Grade and stage of liver disease was also significantly more advanced in the positive group. (P value of: 0.004 and 0.009 respectively). Among the positive group, the most common mode of transmission was intravenous drug use (44.4%). Overall the most common mode of transmission (40.9%) was unknown (most patients were detected during screening for blood donation). There is a clear male predominance in the HDV positive group, that could be due to the higher rate of high risk behavior, including IV drug abuse. Conclusions: On the basis of findings, we suggest that the anti-HDV status in any patient with positive HBsAg should be checked. Confirmation of the results of anti-HDV Ab tests with more accurate tests showing recent HDV infection seems to be essential.

Disorders of Gastrointestinal Motility & Sensation : Subjective Lactose Intolerance In Apparently Healthy Adults In Southern Iran: Is It Related To IBS?

( Mehdi Saberi Firouzi ),( Famaz Khademolhosseini ),( Davood Mehrabani ),( Maryam Yousefi ),( Moosa Salehi ),( Seyyed Taghi Heidary ) 대한소화기학회 2007 SIDDS Vol.9 No.-

Background/Alms: Lactase deficiency leads to maldigestion and malabsorption of lactose. The symptoms of this deficiency in some individuals are abdominal pain and distension, flatulence, and the passage of loose watery stools. This study aimed to investigate the prevalence of subjective lactose intolerance and its relation to Irritable Bowel Syndrome (IBS), demographic factors and living habits. Methods: A survey of 1978 individuals aged above 35 years was conducted in Shiraz, Southern Iran, using a questionnaire that consisted of items regarding demographic data, life style and gastrointestinal symptoms including those experienced after consumption of dairy products. Diagnosis of IBS was made on the basis of Rome II criteria. Results: A total of 562 subjects reported lactose intolerance (28.41%). The prevalence was significantly higher in females, in subjects taking NSAIDs or acetaminophen, and in patients with irritable bowel syndrome. Subjective lactose intolerance was also associated with health-seeking behavior such as restricting diet, using herbal medicine, taking over-the-counter drugs and visiting a physician. On the other hand, no relation was found between lactose intolerance and age, smoking or the number of meals per day. Conclusions: Lactose intolerance has a relatively high prevalence in our region and is associated with irritable bowel syndrome. A test for lactose malabsorption should, therefore, be included in the work-up of patients with this syndrome.

Disorders of Small and Large Intestine : Prevalence of Celiac Disease In Shiraz, Southern Iran

( Mehdi Saberi Firouzi ),( Marzieh Nejabat ),( Gholamreza Omrani ),( Davood Mehrabani ),( Farnaz Khademolhosseini ) 대한소화기학회 2007 SIDDS Vol.9 No.-

Background/Aims: Celiac disease (CD) is an auto immune type of gastrointestinal (GI) disorder, in genetically susceptible persons. Recent reports from Middle East and from our country revealed a high prevalence of disease as high as Europe. However, in our daily practice celiac disease is not a prominent diagnosis, and is considered as a sub-clinical or latent form. This study was performed to evaluate the prevalence of celiac disease in Shiraz, southern Iran. Methods: Serum samples were collected from 1,440 persons (age range=20-83 years, mean age=45.4 years) and screened for endomysial and tissue transglutaminase antibodies. A questionnaire was completed for all subjects in relation to GI symptoms and cases with positive serology were requested to undergo small-bowel biopsy. Results: Five subjects were positive for anti-tTG antibody assay, 4 agreed to undergo biopsy, and three cases were reported with March I histologic findings. In two cases with positive serologic anti-EMA, more advanced forms of CD were present. Conclusions: The prevalence of celiac disease in apparently healthy adults was lower than reported series form northern parts of the country. Therefore, we suggest a more long term follow up study in high risk groups especially in apparently healthy subjects in our region.

Esophageal, Gastric and Duodenal Disorders : The Associated Factors of Gastro-Esophageal Reflux Disease (GERD) in Shiraz, Southern Iran

( Mehdi Saberi Firouzi ),( Famaz Khademolhosseini ),( Maryam Yousefi ),( Davood Mehrabani ),( Najaf Zare ),( Seyed Taghi Heydari ) 대한소화기학회 2007 SIDDS Vol.9 No.-

Aim: Symptoms of gastroesophageal reflux disease (GERD) represent one of the most common problems in the western world. The aim of this study was to determine the prevalence and symptoms of GEFD in a healthy general population in relation to demographic, lifestyle and health-seeking behaviors in Shiraz, southern Iran. Methods: 1978 subjects of >35 years old who referred to Gastroenterohepatology Research Center and completed a questionnaire consisting of 53 questions for GERD in relation to demographic, lifestyle and health-seeking behavior were enrolled in our study during a period of five months. The validity and reliability of the questionnaire were determined. Results: The prevalence of GERD was 15.4%, which was more in females (17.3%), in rural areas (19.8%), in illiterate subjects (21.5%) and in the mean age of 50.25 years. There was significantly a lower prevalence in subjects having fried foods (14.8%), and fruit and vegetables (14.6%). We noticed more symptoms in subject consuming pickles (22.1%), taking Aspirin (21%) and in subjects with psychological distresses. (27.2%) and headaches (22%). The correlation between GERD and halitosis (18.3%), dyspepsia (30.6%), anxiety (19.5%), nightmares (23.9%) and restlessness (18.5%) was statistically significant. Their health seeking behavior showed that there was a significant restriction of diet (20%), consumption of herbal medicine (19%), using over-the-counter drugs (29.9%), and visiting a physician (24.8%). Presence of GERD symptoms was also significantly related to previous family history of the disease (22.3%). Conclusion: GERD was significantly more in females, rural and illiterate subjects and had a correlation with consumption of pickles, headache, psychological distress, dyspepsia, halitosis, anxiety, nightmare, restlessness and pervious history of GERD in the family and aspirin intake but the correlation was inverse with consumption of fat and fiber intake.

Disorders of Liver : In Vivo Protoscolicidal Effect Of Hypertonic Glucose On Protoscolices Of Hydatid Cyst

( Syed Vahid Hosseini ),( Seyed Mahmoud Adjjadi ),( Davood Mehrabani ),( Kurosh Ghanbarzaden ),( Zahra Barzin ),( Nader Tanideh ),( Masoud Amini ) 대한소화기학회 2007 SIDDS Vol.9 No.-

Background/Aims: The treatment of choice for Hydatidosis is surgery, but before evacuation of a cyst, protoscolicidal agents are needed to be injected to prevent a secondary cyst formation and the spreading of the disease. This study was performed to determine the protoseolicidal effect of various concentrations of hypertonic glucose in an animal model infected to hydatid cyst. Methods: Sheep liver hydatid cyst surface were sterilized by heat and the cyst contents were evacuated completely and transferred into falcon tubes, where protoscolices were precipitated and separated. The viability of protoseolices was determined by using eosin stain method. A total of 1500 protoscolices were exposed to 0.5% cetrimide, 0.5% silver nitrate, 0.9% and 20% sodium chloride and 10%, 15%, 25%, and 50% concentrations of glucose with exposure times of one, 2 and 5 minutes and were injected into the peritoneal cavity of 5 BALB/c mice for each agent. The animals were kept for 9 months and then were autopsied. Blood sample for countercurrent immunoelectrophoresis (CCIEP) was provided and the animals were examined for presence of any cyst in peritoneal cavity and recorded. Results: A total of 40 BALB/C mice were used in all exposure times while 24, 21 and 32 mice survived when exposed to different protoscilicidal agents after 1, 2 and 5 minutes respectively. None of the mice were infected to cysts after exposure to 50% hypertonic glucose solution. Identical to 50% glucose solution no infection was noticed for 0.5% cetrimide and 20% sodium chloride solutions too. The CCIEP results of animals indicating to cyst formation confirmed the presence of antibody in the circulation. Conclusions: Similarity of 50% glucose solution and cetrimide and 20% sodium chloride in complete killing of protoscolices present it as potent protoscolicidal agent and the side effects of cetrinide and hypertonic sodium chloride indicate to further studies on probable side effects.

Esophageal, Gastric and Duodenal disorders : Migraine Headache And Peptic Ulcer Diseased In Children

( Mohammad Hadi Imanieh ),( Mahrnood Haghighat ),( Seved Mohsen Dehghani ),( Maziyar Irani ),( Maryam Yousefi ),( Davood Mehrabani ) 대한소화기학회 2007 SIDDS Vol.9 No.-

Background/Aims: Although correlation between migraine headache and GI symptoms were reported, they did not significantly support the correlation between migraine headache and mucosal inflammation or peptic ulcer diseases. This study aimed to find such a correlation. Methods: There were 70 patients (5-15 years old) who had endoscopic documented mucosal inflammation or peptic ulcer diseases. Frequency of migraine headache in this group and a group consisting of a sample of normal population (300) who had not any GI problem in the past year was determined by a questionnaire. Results: The mean age of patients was 10.34 years with male to female ratio of 4:3. Frequency of migraine headache, esophagitis, gastritis, gastric ulcer, duodenitis, and duodenal ulcer was 22.9%, 68.6%, 92.9%, 4.3%, 24.3% and 4.3%, respectively. Regarding frequency of migraine headache among the normal population which was 12.4%, the frequency of migraine headache among patients with mucosal inflammation and peptic ulcer diseases of the upper GI tract, which was 22.9% showed significant statistical correlation. A significant correlation was seen between migraine and esophagitis and also between migraine headache and gastritis. Conclusions: The presence of significant correlation between migraine headache and mucosal inflammation or peptic ulcer diseases of the upper GI tract supports some of precious studies suggested this correlation.

Disorders of Liver : The Role Of Germinative Layer In Inducing Hydatid Cyst

( Seyed Vahid Hosseini ),( Soheal Mansoorian ),( Seyed Mahmood Sadjjadi ),( Davood Mehrabani ),( Zahra Barzin ),( Nader Tanideh ),( Afshin Amini ),( All Rafati ),( Masoud Amini ) 대한소화기학회 2007 SIDDS Vol.9 No.-

Background/Aims: In spite of use of protoseololeidal agents during surgery, a notable rate of recurrence of disease in post-operation patients is still observed. The question exists is that alive scolices would cause any recurrence or is due to the presence of the germinative layer. The aim of this study was to evaluate the effect of scolicidal agents on this layer as well as its ability of the layer to produce any hydatid disease in an in vivo condition. Methods: Germinative layer of hydatid cyst was separated under sterile condition, was divided into 0.5 cm parts, exposed to various protoseolicidal agents for 2 minutes, and implanted in peritoneal cavity of 62 BALB-C mice. After 9 months, the peritoneum was evaluated macroscopically and microscopically for the presence of any hydatid disease. Results: After 9 months, no hydatid disease was visible in the peritoneum of all the sixty two mice. Conclusions: In this study, the germinative layer could not cause any hydatid disease but positive results of Tarakanovet al. with cultures of protoseolices and the germinative layer cells in the peritoneal cavity mice indicate to a need of another study of implantation of culture cells of the germinated layer lacking protoscolices in a larger animal study to clarify the differences.

In vitro protoscolicidal effects of hypertonic glucose on protoscolices of hydatid cyst

Seyed Vahid HOSSEINI,Kurosh GHANBARZADEH,Zahra BARZIN,Seyed Mahmoud SADJJADI,Nader TANIDEH,Davood MEHRABANI 대한기생충학열대의학회 2006 The Korean Journal of Parasitology Vol.44 No.3

Evaluation of a Polycaprolactone/Gelatin/Lucilia sericata Larva Extract Nanofibrous Mat for Burn-Wound Healing

Seyedeh‑Sara Hashemi,Zhila Hayatdavoodi,Mehdi Kian,Nahid Hassanzadeh Nemati,Davood Mehrabani,Ali‑Akbar Mohammadi,Alireza Rafati,Mojtaba Ghaedi,Behzad Ghafari,Adnan Alizadeh Naini 한국섬유공학회 2023 Fibers and polymers Vol.24 No.11

Electrospun nanofibrous mats have shown great potential for dressing skin wounds. In this study, a nanofibrous mat composed of polycaprolactone (PCL), Gelatin (GLT), and Lucilia sericata larva extract (LSLE) was fabricated by the electrospinning method. The morphology of the fabricated nanofibrous mat and its morphological properties (fibers distribution frequency, fiber diameter, pore area, number of pores, and intersection density) were evaluated by scanning electron microscopy (SEM) and ImageJ software. The PCL/GLT/LSLE nanofibrous mat had well-branched and porous structure with a mean diameter of 500.2 ± 20.46. The presence of functional groups of PCL and GLT polymers in the structure of the PCL/GLT/LSLE mat was confirmed by Fourier-transform infrared spectroscopy (FTIR). Contact angle measurement and swelling behavior assessment showed that the PCL/GLT/LSLE mat had better surface wettability (75.67 ± 2.71) and hydrophilicity (241.8 ± 27.01) properties in comparison with the PCL and PCL/GLT mats. Findings from the MTT assay indicated the biocompatibility of the PCL/GLT/LSLE mat for human dermal fibroblasts (HDFs). In addition, the anchorage and proliferation of HDFs on the PCL/GLT/LSLE mat was confirmed by SEM. Macroscopic and histopathological evaluations were performed at the end of the 3, 7, and 14 days after the conduction of experimental burn wound injury in rats. The results indicated that grafting the skin wounds with the PCL/GLT/LSLE nanofibrous mat accelerated the wound closure and improved histopathological score compared to the other animal groups dressed with the PCL and PCL/GLT mats. In conclusion, the PCL/GLT/LSLE nanofibrous mat has promising potential for dressing skin wounds.