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Shahanaz Sultana,Chai Ling Ho,Suhaimi Napis,Veronika,Parameswari Namasivayam 한국작물학회 2014 Journal of crop science and biotechnology Vol.17 No.4
9-Cis-epoxycarotenoid dioxygenase (NCED) is an important enzyme which catalyzes the oxidative cleavage of abscisic acid(ABA), a plant growth regulator which plays a vital role in stress tolerance. In this study, a cDNA sequence encoding NCED from amangrove plant (Acanthus ebracteatus) was analyzed and overexpressed in rice. Analysis of the deduced amino acid sequence ofAeNCED revealed an open reading frame of 1,638 bp encoding a protein of 545 amino acids, with a 123 bp 5’-untranslated region(UTR) and a 259 bp 3’-UTR. The deduced amino acid sequence of AeNCED is more than 80% identical to the amino acid sequencesof carotenoid cleavage dioxygenase from carrot, tomato, and coffee. The RNA encoding AeNCED was detected in transgenic rice(Oryza sativa cv. BRRI dhan29) plants overexpressing this cDNA. These plants only showed significantly higher tolerance to salinityat germination and better performance at seedling stages. The levels of ABA in transgenic rice seedlings overexpressing AeNCEDtreated with 100 mM NaCl for 24 hours were higher than those of untransformed plants. However, a higher level of dihydrophaseicacid (DPA) and ABA glucose ester (ABA-GE) were also observed in these transgenic plants suggesting that rapid degradation ofABA through a self-regulation mechanism
신재생에너지 자원 관리를 위한 센서 네트워크 기반 모니터링 시스템 설계
박성규 ( Seong Kyu Park ),왕령 ( Ling Wang ),이양구 ( Yang Koo Lee ),채덕진 ( Duck Jin Chai ),허철호 ( Chul Ho Heo ),류근호 ( Keun Ho Ryu ),김광득 ( Kwang Deuk Kim ) 한국정보처리학회 2009 한국정보처리학회 학술대회논문집 Vol.16 No.1
최근 환경 문제와 고유가 시대에 따른 문제가 전세계적인 이슈로 떠오르면서, 신재생에너지 자원의 개발에 대한 필요성이 대두되고 있다. 이와 함께 신재생에너지 자원에 대한 국내외 연구가 활발히 진행되고 있다. 최근에는 IT 기술이 발달함에 따라 센서와 같은 최신기술을 신재생에너지 자원 관리를 위한 모니터링에 적용하고 있다. 이러한 환경에서 실시간적이고 무제한적인 신재생에너지 자원 정보를 표현하기 위한 기술과 센서의 특성에 맞는 시스템 구조가 필요하다. 이 논문에서는 효율적인 신재생에너지 자원 관리를 위한 센서 네트워크 기반 모니터링 시스템 구조를 설계한다.
Genome Constitution and Classification Using Retrotransposon-Based Markers in the Orphan Crop Banana
( Chee How Teo ),( Siang Hee Tan ),( Chai Ling Ho ),( Qamaruz Zaman Faridah ),( Yasmin Rofina Othman ),( John Seymour Heslop Harrison ),( Ruslan Kalendar ),( Alan Howard Schulman ) 한국식물학회 2005 Journal of Plant Biology Vol.48 No.1
We have exploited the repetitive and dispersed nature of many long terminal repeat (LTR)-retrotransposon families for characterizing genome constitutions and classifying cultivars of the genus Musa. Insertional polymorphisms of the elements were studied using seven published and two newly designed primers facing outwards from the LTRs and reverse transcriptase (RT) domain of the retrotransposon. The primers generated specific amplification patterns showing the universal applicability of this marker type. The Inter-Retrotransposon Amplified Polymorphism (IRAP) markers distinguished the A and B genomes of the banana species (Musa acuminata Colla and Musa balbisiana Colla) and between banana cultivars. The IRAP markers enabled phylogenetic analysis of 16 Malaysian banana cultivars and determination of the genome constitution of hybrid banana (AAB, ABB, AABB, and AAAB), and gave information about ancestral genotypes of the hybrids. In addition, the TRAP detected new retrotransposon insertions into the genome of tissue culture regenerants. This PCR-based IRAP assay is amenable to large-scale throughput demands in screening breeding populations and is applicable for any crop.