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Impaired nucleolus biogenesis during pre-implantation development of mouse nuclear transfer embryos
Zhong Zheng(정중),Seul-Ki Lee(이슬기),Xiang-Shun Cui(최향순) 충북대학교 동물생명과학연구소 2013 동물생명과학연구 Vol.5 No.-
핵인의 가장 널리 알려진 기능 중에 하나는 리보솜의 합성을 수행한다는 것이다. 접합 유전자의 수정 이후에 핵인의 재구축은 포유 동물의 착상 전 배아의 성공적인 발달을 위해 필수 적인 요소이다. 그러나 핵 이식 배아에서 핵인의 발달이 지연 되거나 비정상적으로 발달하는 양상을 보인다. 이번 연구에서는 쥐의 난자를 이용해 정자미세주입법과 단위발생 그리고 난구세포를 이용해 핵이식을 수행했을 각각의 경우에 착상 전 배아가 발달하는 동안 핵인의 발달과 핵인의 전구체 형성 그리고 핵인의 미세구조와 핵인을 조절하는 부위의 활성화 정도를 살펴보고 비교해보기 위한 실험을 수행하였다. 결과를 살펴보면 난구세포를 이용해 핵이식을 수행한 난자에서는 정자미세주입법과 단위발생 시킨 난자보다 전핵당 전구체의 개수가 더 많은 결과를 보였다. 그리고 난구세포를 이용하여 핵이식을 수행한 난자의 4cell 단계에서 손상된 핵인이 활성화 되는 것을 살펴볼수 있었고 미세구조 단계에서부터 질이 덜 좋은 핵인이 발달하고 또 한 염색체상에서 핵인을 조절하는 부위의 활성화 정도가 떨어지는 것을 살펴볼 수 있었다. 그리고 난구세포를 이용하여 핵이식을 수행한 난자의 경우 정자미세 주입법이나 단위 발생 난자에 비해 배반포 형성 비율이 억제되는 것을 살펴볼 수 있었다. 결론적으로 핵이식 난자에서 손상된 핵인의 발달이 수행되며 핵이식 된 난자의 착상 전 발달 능력이 현저히 떨어지는 데 영향을 미치는 요인중에 하나가 핵이식 된 난자에서 손상된 핵인이 형성되기 때문이라고 설명할 수 있다. The most well-known function of nucleolus is ribosome biogenesis. The re-establishment of functional nucleoli after zygotic genome activation is essential for successful development of mammalian pre-implantation embryos. However, in somatic cell nuclear transfer (SCNT) embryos, the nucleoli always showed delayed developed or malfunction. In current study, mouse intracytoplasmic sperm injection (ICSI), pathenogenetic activated (PA) and cumulus cell nuclear transfer (CCNT) embryos were generated to compare the nucleoli development during pre-implantation development, including nucleolar precursor bodies formation, nucleolar ultrastructure and active nucleolar organizer region (NOR) numbers. The results showed that, CCNT embryos had more nucleolar precursor bodies (NPBs) per pronucleus than ICSI and PA embryos at pronuclear stage (2.82 v.s. 0.98 and 1.19). At 4-cell stage, the CCNT embryos showed impaired nucleolar activity, including inferior nucleolar development at ultrastructure level and less active NORs on chromosomes (6.68 v.s. 7.44 and 7.56). Moreover, CCNT embryos showed significant lower blastocyst rate than ICSI and PA embryos (62.0% v.s. 97.1% and 98.0%). The results demonstrated that mouse CCNT embryos had impaired nucleolar development, which may account for their low pre-implantation developmental competence.
ZHENG, Zhong,ZHAO, Ming-Hui,JIA, Jia-Lin,HEO, Young-Tae,CUI, Xiang-Shun,OH, Jeong Su,KIM, Nam-Hyung The Society for Reproduction and Development 2013 The Journal of reproduction and development Vol.59 No.6
<P><B>Abstract</B></P><P> A number of germ cell-specific transcription factors essential for ovarian formation and folliculogenesis have been identified and studied. However, the role of these factors during early embryonic development has been poorly explored. In the present study, we investigated the role of SEBOX, a maternal homeobox transcription factor, during early embryonic development in porcine parthenotes. mRNA for <I>SEBOX</I> is preferentially expressed in oocytes, and expression persists until embryonic genome activation (EGA). Knockdown of SEBOX by siRNA disrupted early embryonic development, but not oocyte maturation. Many maternal genes essential for early embryonic development were upregulated in SEBOX-depleted embryos. Moreover, some pluripotency-associated genes, including <I>SOX2</I> and <I>NANOG</I>, were upregulated when SEBOX was knocked down. Therefore, our data demonstrate that SEBOX is required for early embryonic development in pigs and appears to regulate the degradation of maternal transcripts and the expression of pluripotency genes.</P>
Zheng Gui Zhong,Kim Bo-Yeon,Yoon Hyung-Joo,Wei Ya Dong,Xijie Guo,Jin Byung-Rae,Shon Hung-Dae Korean Society of Sericultural Science 2007 International Journal of Industrial Entomology Vol.14 No.1
In a previous study, three larval cuticle protein genes were cloned from the mulberry longicorn beetle, Apriona germari (Comp. Biochem. Physiol. B 136, 803-811, 2003). In the present study, the genomic structures of these three larval cuticle protein genes (AgLCP9.2, AgLCP12.6 and AgLCP12.3) were elucidated. All three cuticle protein genes consist of one intron and two exons. Southern blot analysis of genomic DNA suggested that three cuticle protein genes are a single copy gene. In addition, a novel larval cuticle protein gene, AgLCP10.6, was cloned from A. germari in this study. The AgLCP10.6 cDNA contains an ORF of 300 nucleotides that are capable of encoding a 100-amino acid polypeptide with a predicted molecular mass of 10.6 kDa. The amino acid sequence deduced from the AgLCP10.6 cDNA contained a type-specific consensus sequence identifiable in other insect cuticle proteins and is most homologous to Drosophila melanogaster cuticle protein ACP65A (51 % protein sequence identity). Northern blot analysis revealed that AgLCP10.6 showed epidermis-specific expression.
Third Party Logistics Service Provider Selection : Application of the Improved Aggregate Cost Method
Zheng-Zhong Ren 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.9
During the process of enterprise management, those chemical enterprises usually adopt outsourcing, as they hand over the logistical work to the third party. Here, we call the third part the “logistics service provider”. In this paper, Chemical Enterprise A is set as an example to study the application of the optimizing selection for the third party logistics service provider, as well as its improvement of aggregate cost method. This paper is aimed at theory, practicality and operability, and hoping to choose the provider which can guarantee the cost is smallest by introducing the original provider model. Among all the aspects, this paper is persuasive. Meanwhile, due to the instability of the relevant data which are collected, the factors impacting upon the total cost are uncertain, thus their credibility is affected. Later, according to the actual demand of the logistics service provider as well as the Benefit-Cost Ratio in aggregate cost method, the qualitative method and the quantitative method are combined with each other, and then the cost can be reduced through Analytic Hierarchy Process. Moreover, the factors which are irrelevant to cost selection are reasonably transformed into the cost adjustment proportion, and an improved provider selection model is established. At last, by introducing the specific case of the third party logistics service provider of Chemical Enterprise A, the effectiveness in practical application of improving the provider selection model can be verified.