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( Xiang Xiao ),( Feng Ping Wang ),( Xu Ke ),( Xi Xiang Tang ),( Ying Bao Gai ),( Muhammad Aamer Mehmood ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.3
A psychrotrophic bacterium, Arthrobacter sp. ON14, isolated from Antarctica, was shown to exhibit a high β-galactosidase activity at a low temperature. A genomic library of ON14 was constructed and screened for β-galactosidase genes on functional plates containing 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) as the substrate. Two different β-galactosidase genes, named as galA, galB, were found in ON14. Computational analyses of the genes revealed that the encoded protein GalA belongs to family 2 of glycosyl hydrolysases and is a cold-active protein, whereas GalB belongs to family 42 of glycosyl hydrolysases and is a mesophilic protein. Reverse transcription analyses revealed that the expression of galA is highly induced at a low temperature (4℃) and repressed at a high temperature (28℃) when lactose is used as the sole carbon source. Conversely, the expression of galB is inhibited at a low temperature and induced at a high temperature. The purified GalA showed its peak activity at 15℃ and pH 8. The mineral ions Na+, K+, Mg2+, and Mn2+ were identified as enzyme activators, whereas Ca2+ had no influence on the enzyme activity. An enzyme stability assay revealed that the activity of GalA is significantly decreased when it is incubated at 45℃ for 2 h, and all its activity is lost when it is incubated at 50℃.
Xiang Ying Chen,Yan Wu Zhu,Zhi Guo Qiu,Zhong Jie Zhang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.08
How to simply adjust the porosity of carbon materials and largely elevate the capacitive performance of supercapacitors remains interesting but challenging for us. In the present work, we have realized the two purposes by the following steps: firstly, introducing MgO as hard template into potassium hydrogen phthalate can result in the formation of hierarchical pore structures containing micropores and mesopores, whilst individually carbonizing potassium hydrogen phthalate leads to nearly complete micropores; secondly, incorporating rutin as effective redox additive into H2SO4 electrolyte can largely improve the capacitances as well as the energy densities by the gain/loss of two electrons and two protons. For example, the capacitances can increase 1.92 fold when carried out in a two-electrode system. Furthermore, adding 0.15 mmol L -1 rutin into 1 mol L -1 H2SO4 can achieve the maximum energy density up to 20.84Wh kg -1 towards the MgO-templated carbon materials. More importantly, it is also inferred that higher porosity of carbon materials indeed benefits for obtaining larger pseudocapacitive efficiency. Thereby, understanding the matching degree of redox additive's size and that of pore within carbon matrix will help us facilitate the capacitive increase of supercapacitors.
Effects of Lifelong Aerobic Exercise on Ferroptosis-Related Gene Expressions in Kidney of Aged Mice
Ying-Ying Xiang,백경완,원종화,박윤정,김지석 한국운동생리학회 2023 운동과학 Vol.32 No.4
PURPOSE: This study sought to explore the impact of continuous aerobic exercise throughout life on the expression of ferroptosis-related genes in aging kidneys, elucidating the role of exercise in maintaining kidney health. METHODS: Male C57BL/6 mice were divided into young control, young exercise, old control, and old exercise groups, with the latter providing access to spontaneous running wheels. Aged mice were observed until 99 weeks, while young mice were followed until 22 weeks. Kidney tissue samples underwent analysis through quantitative polymerase chain reaction, iron assays, histological staining, and pathological damage scoring. RESULTS: Expression of Nphs1 and Nphs2 exhibited no significant changes after exercise. However, histological analysis revealed that age-related kidney damage, including glomerular mesangial proliferation, was notably offset by lifelong aerobic exercise. The expression of Slc7a11 and Gpx4 remained unaffected by exercise, whereas Nfe2l2 expression decreased significantly in the young exercise groups. Acsl4 expression exhibited no aging- or exercise-induced differences. Additionally, Tfrc expression was lower in the young exercise group than in the young control group but remained consistent between the old and old exercise groups. Total iron (Fe) increased in aged mice compared to that in the young-exercise group, and the Fe2+/Total Fe ratio was lower in the old-exercise than in the old control group. CONCLUSIONS: Lifelong aerobic exercise positively impacted kidney health by enhancing antioxidant capacity and mitigating iron accumulation. These findings underscore the significance of exercise in preventing age-related deterioration in kidney function
Ying, Xi-Xiang,Li, Hai-Bo,Chu, Zheng-Yun,Zhai, Yan-Jun,Leng, Ai-Jing,Liu, Xun,Xin, Chun,Zhang, Wen-Jie,Kang, Ting-Guo 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.7
To investigate the antioxidant effect of vitexin-4"-O-glucoside, a flavone glycoside, isolated from the leaves of Crataegus pinnatifida Bge. var. major, we developed a simple and sensitive high-performance liquid chromatography (HPLC) method to determine levels of malondialdehyde (MDA) in ECV304 cell culture medium after induction by tert-butyl-hydroperoxide (TBHP). The preparation of analyzed samples involved a one-step derivatization with thiobarbituric acid (TBA). HPLC analysis was performed on a $Synergi^{TM}$ Hydro-RP, a polar end-capped $C_{18}$ column ($250{\times}4.6\;mm$, $4\;{\mu}m$), using an acetonitrile-ammonium acetate aqueous solution (10 mM, pH 6.8) as the mobile phase under linear gradient conditions with UV detection at 532 nm. The calibration curve was linear over $0.0125-1.25\;{\mu}M$ MDA (r=0.9951). Relative standard deviations (RSDs) of intra-day and inter-day precision were less than 6.1% and 5.0%, respectively. The mean recovery was $96.9\;{\pm}\;1.6%$. The lower limit of quantification (LLOQ) of MDA was $0.0125\;{\mu}M$. This chromatographic method was successfully applied to investigating the in vitro antioxidant effect of vitexin-4"-O-glucoside. Vitexin-4"-O-glucoside (120 M) protected ECV304 cells from peroxidation induced by TBHP.
도라지 잎 홍차 및 녹차 제품의 항산화활성 폴리페놀과 플라보노이드 비배당체 함량
Xiang Min Piao,Ying Yu,Sin Hee Han,Sang Won Lee,Seon Woo Cha,Ying Ping Wang,Jing Guo 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : Invitro antioxidant activity, polyphenol and flavonoid aglycone contents in black and green tea products of balloon flower leaves were investigated to provide valuable information for the further development and utilization of resources of Platycodon grandiflorum. Methods and Results : Flavonoid aglycone contents were investigated using HPLC (SHIMADZU, Japan) with a hypersil ODS column (125 mm × 4 mm, 5-μm particle, HP). DPPH and ABTS radical scavenging activities were measured by method of Lee & Lee (2004) with slight modification. Antioxidant activity, polyphenol and flavonoid contents in green tea were significantly higher than these in black tea. PC analysis indicated that first principal components explained 79.9% of the total variability for five traits investigated. PC2 explained 19.7% of the variation. Conclusion : It can be concluded from these results that these characteristics can reveal the active compound variation of black and green tea products of balloon flower leaves. These results provide scientific evidence for the utilization of balloon flower leaves.
Xi-xiang Ying,Hai-bo Li,Zheng-yun Chu,Yan-jun Zhai,Ai-jing Leng,Xun Liu,Wen-jie Zhang,Ting-guo Kang,Chun Xin 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.7
To investigate the antioxidant effect of vitexin-4"-O-glucoside, a flavone glycoside, isolated from the leaves of Crataegus pinnatifida Bge. var. major, we developed a simple and sensitive high-performance liquid chromatography (HPLC) method to determine levels of malondialdehyde (MDA) in ECV304 cell culture medium after induction by tert-butyl-hydroperoxide (TBHP). The preparation of analyzed samples involved a one-step derivatization with thiobarbituric acid (TBA). HPLC analysis was performed on a SynergiTM Hydro-RP, a polar end-capped C18 column (250×4.6 mm, 4 μm), using an acetonitrile-ammonium acetate aqueous solution (10 mM, pH 6.8) as the mobile phase under linear gradient conditions with UV detection at 532 nm. The calibration curve was linear over 0.0125-1.25 μM MDA (r = 0.9951). Relative standard deviations (RSDs) of intra-day and inter-day precision were less than 6.1% and 5.0%, respectively. The mean recovery was 96.9 ± 1.6%. The lower limit of quantification (LLOQ) of MDA was 0.0125 μM. This chromatographic method was successfully applied to investigating the in vitro antioxidant effect of vitexin-4"-O-glucoside. Vitexin-4"-O-glucoside (120 M) protected ECV304 cells from peroxidation induced by TBHP.
Han Ying-Hao,Mao Ying-Ying,Feng Yao-Yuan,Xiang Hong-Yi,Sun Hu-Nan,Jin Mei-Hua,Kwon Taeho 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.3
In this study, we performed RNA sequencing of Prx II +/+ and Prx II −/− dermal mesenchymal stem cells (DMSCs) to identify differentially expressed genes (DEGs). To explore the role of Prx II in DMSCs, we performed Gene Ontology analysis of the DEGs. The results showed that the DEGs were mainly involved in the biological processes of cell migration, intercellular adhesion, and coordination of the regulation of stem cell homing. Through the construction of protein–protein interaction network, four hub genes Cd274 , Ccl5 , Il1b , and Stat1 involved in cell adhesion and cell homing were screened. Quantitative reverse transcription PCR analysis showed that Cd274 , Ccl5 , Il1b , and Stat1 were down regulated in Prx II −/− DMSCs. miRwalk and Starbase databases were further used to screen the upstream molecules miRNA and lncRNA regulating hub gene. Prx II was found to be involved in the regulation of stem cell homing via the Tctn2/miR-351/Stat1/Il1b axis. Thus, we demonstrated that Prx II is a key molecule in the regulation of the homing ability of DMSCs. Our results provide a theoretical foundation for improving the homing ability of DMSCs by targeting Prx II.