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Takehisa Matsumoto,Masatomo Kawakubo,Mayumi Shiohara,Toshiko Kumagai,Eiko Hidaka,Kazuyoshi Yamauchi,Kozue Oana,Kenji Matsuzawa,Hiroyoshi Ota,Yoshiyuki Kawakami 한국미생물학회 2009 The journal of microbiology Vol.47 No.2
“Helicobacter heilmannii” is an uncultivable spiral-shaped bacterium inhabiting the human gastric mucosa. It is larger and more tightly-coiled than H. pylori. We encountered a patient with chronic gastritis infected a “H. heilmannii”-like organism (HHLO), designated as SH6. Gastric mucosa derived from the patient was orally ingested by specific pathogen free mice. Colonization of the mice by SH6 was confirmed by electron microscopy of gastric tissue specimens. In an attempt to characterize SH6, 16S rRNA and urease genes were sequenced. The 16S rRNA gene sequence was most similar (99.4%; 1,437/1,445 bp) to HHLO C4E from a cheetah. However, the urease gene sequence displayed low similarity (81.7%; 1,240/1,516 bp) with HHLO C4E. Taxonomic analysis disclosed that SH6 represents a novel strain and should constitute a novel taxon in the phylogenetic trees, being discriminated from any other taxon, with the ability of infecting human gastric mucosa.
Takehisa Oikawa 경북대학교 환경과학연구소 1993 環境科學硏究所論文集 Vol.7 No.1
Primary production, which is the production by sutotrophic organisms, plays an essential role in all ecosystems. It determines the size and the activity of an ecosystem, depending on the flow of matter and energy from producers through herbivores to carnivores. Even humans, who are omnivores in an ecosystem, cannot escape this restriction. The princeipal ecosystems on land are forest and grassland ecosystems. As can easily be understood, forest ecosystems store a huge amount of organic matter mainly in woody parts. Whittaker and Likens (1973) estimated the total biomass of forests to be 1590 x 10'5 g d.w.; forest ecosystems, which occupy about 1/3 of the total land area, amount to about 87% of the total terrestrial biomass. In contrast, grassland ecosystems, including natural grasslands and arable lands, constitute a minor portion of the total biomass. Why then do humans depend on the major part of their food being produced not from arboreal crops, but from herbaceous crops? As is well known, the three major crops in the world are rice, wheat and maize. This question can be solved on the basis of mathematical models such as the one developed by Monsi and Saeki (1953), which gives primary productivity as a function of the physiological characteristics of leaves, the ecological characteristics of a plant community and an environmental parameter. A tentative hypothesis will be presented using their mathematical model.
Fuzzy Fault Tree Analysis with Natural Language
Onisawa, Takehisa Korean Institute of Intelligent Systems 1997 한국지능시스템학회논문지 Vol.7 No.1
This paper mentions a fault tree analysis using not probability but natural language and fuzzy theory, Reliability estimate of each basic event and dependence level estimate among subsystems are expressed by linguistic terms. Analysis results are also expressed by natural language. The meaning of linguistic terms is expressed by a fuzzy set. In the presented analysis approach parametrized operations of fuzzy sets are considered so that analyst's subjectivity can be introduced into the analysis. This paper gives the Chernobyl accident as an example of the fuzzy fault tree analysis using linguistic terms.
IKEDA, TAKEHISA,GRIMME, STEPHEN,Ma, NING,IKEDA, SEACHIRO,FIENO, ANGELA,IVES, DAVID H,GUO, SHENYUAN,PARK, INSHIK 東亞大學校附設遺傳工學硏究所 1997 遺傳工學硏究 Vol.- No.4
Three of the four deoxynucleoside kinases required for growth of Lactobacillus acidophilus R-26 exist as heterodimeric pairs specific for deoxyadenosine(dAK) and deoxycytidine (dCK) or dAK and deoxyguanosine (dGK). However, only two tandem genes, dak/dgk, are found, and are expressed only as dAK/dGK in transformed Escherichia coli. Sequencing peptides spanning 63% of the native dCK subunit revealed a sequence identical to that deduced from dgk(beginning MTVIVL…), except that dCK lacks residues 2 and 3 (dCK is M-IVL;dGK is ·TVIVL). Also, mass spectrometry indicates that native dCK and dGK subunits are identical in mass adjusted for the first three residues. Furtheromore, the native enzymes have identical isoelectric pH values, indicating an equal number of charged residues. To enable E. coli to express peptide having the native dCK sequence, codons 2 and 3 were deleted from the dgk portion of the tandem genes, resulting in expression of protein having the specificities and regulatory properties of dAK/dCK, including heterotropic stimulation of dAK activity by deoxycytidine or dCTP (not deoxyguanosine or dGTP) and end-product inhibition of the respective activities by dATP and dCTP. Subcloning normal and mutant dgk yielded homodimeric dGK and dCK, respectively. The dCK homodimer strongly resembles human dCK with a low ?? for deoxycytidine, the ability to phosphorylate deoxyadenosine and deoxyguanosine at much higher ?? values, and end-product inhibition by dCTP. Thus two distinct and specific enzymes evidently are derived from a single Lactobacillus gene. The mechanism by which this occurs in vivo has yet to be elucidated.