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An Oligonucleotide Microarray Bait for Isolation of Target Gene Fragments
Shi, Rong,Ma, Wen-li,Liu, Cui-Hua,Song, Yan-Bin,Mao, Xiang-Ming,Zheng, Wen-Ling Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.2
A new molecular-baiting method was studied by retrieving targeted gene fragments from an oligonucleotide microarray bait after hybridization. To make the microarray bait, 70-mer oligonucleotides that were designed to specifically represent the SSA1 gene of Saccharomyces cerevisiae were printed on the slide. Samples of the Saccharomyces cerevisiae mRNA were extracted and labeled by the RD-PCR (Restriction Display PCR) method using the Cy5-labelled universal primer, then applied for hybridization. The sample fragments that hybridized to the microarray were stripped, and the eluted cDNAs were retrieved and cloned into the pMD 18-T vector for transformation, plasmid preparation, and sequencing. BLAST searching of the GenBank database identified the retrieved fragments as being identical to the SSA1 gene (from 2057-2541bp). A new method is being established that can retrieve the sample fragments using an oligo-microarray-bait.
An Oligonucleotide Microarray Bait for Isolation of Target Gene Fragments
( Shi Rong ),( Ma Wen Li ),( Liu Cui Hua ),( Song Yan Bin ),( Mao Xiang Ming ),( Zheng Wen Ling ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.2
A new molecular-baiting method was studied by retrieving targeted gene fragments from an oligonucleotide microarray bait after hybridization. To make the microarray bait, 70-mer oligonucleotides that were designed to specifically represent the SSAl gene of Saccharomyces cerevisiae were printed on the slide. Samples of the Saccharomyces cerevisiae mRNA were extracted and labeled by the RD-PCR (Restriction Display PCR) method using the CyS-labelled universal primer, then applied for hybridization. The sample fragments that hybridized to the microarray were stripped, and the eluted cDNAs were retrieved and cloned into the pMD 18-T vector for transformation, plasmid preparation, and sequencing. BLAST searching of the GenBank database identified the retrieved fragments as being identical to the SSAl gene (from 2057-2541bp). A new method is being established that can retrieve the sample fragments using an oligo-microarray-bait.
Integrating Care and Respect: Early Confucian Ethics as Inclusive Ethics
( Shirong Luo ) 성균관대학교 유교문화연구소 2021 儒敎文化硏究(中文版) Vol.0 No.35
What it is commonly referred to as “early Confucian ethics” has its textual sources in two canonical Confucian texts-the Analects and the Mencius, and to a lesser extent, in the Xunzi. This article breaks fresh ground in the study of early Confucian ethics by defending a new interpretation that Confucian ethics is an inclusive ethics in the sense that all of its key notions contain the dual dimensions of care and respect. I call this “the inclusion thesis.” This paper will proceed as follows. First, I make some general remarks about the importance of integration of care and respect in ethics. Second, I distinguish between two ways of making ethics inclusive-(1) the integration by reduction and (2) the integration by complementation. Between the two, I suggest that the method of integration by complementation should be preferred. Third, I present two case studies to illustrate the importance of inclusivity of care and respect. Lastly, by meticulous exegetical analysis, I attempt to substantiate my inclusion thesis that early Confucian ethics is a moral theory in which care (or love) and respect are conceptually amalgamated through the complementary integration.
Shirong Huang,Min Tang,Fenfen Chen,Shengnan Zhao,Dongfang Chen 한국축산식품학회 2024 한국축산식품학회지 Vol.44 No.2
The color, lipid oxidation, heme iron (HI) and non-heme iron (NHI) contents, metmyoglobin content and Soret band of myoglobin of ground pork subjected to supercritical CO2 treatment under different conditions, or to heat treatment (40℃, 2 h) and subsequent storage at 4℃ were evaluated during 9-day period. Supercritical CO2 treatment significantly increased CIE L* and CIE b* values of ground pork during subsequent storage, while the HI content was slightly affected. In general, CIE a* value and metmyoglobin content were decreased. Supercritical CO2 treatment for 2 h could increase the thiobarbituric acid-reactive substances (TBARS) value, while treatment for 1 h or less had no effect. The NHI content could be increased only after treatment at above 40℃ or 17.2 MPa for 2 h. The Soret band of myoglobin was shifted to longer wavelength. Increasing treatment temperature from 35℃ to 45℃ could increase CIE L*, CIE a*, CIE b* and TBARS values, HI and NHI contents of the ground pork, while decreasing metmyoglobin content. As the treatment pressure increased from 13.8 MPa to 20.7 MPa, CIE b* and TBARS values were decreased, while the NHI and metmyoglobin contents were increased. However, the other parameters were unchanged. Extending exposure time from 0.5 h to 2 h could increase CIE L*, CIE b* and TBARS values, HI contents, while decreasing CIE a* value and metmyoglobin content. Correlation analysis showed that the TBARS value was significantly and negatively correlated with the HI content or metmyoglobin content in samples treated at 40℃ or above for 2 h.
Tingfeng Tan,Shirong Wang,Xianggao Li,Caiyan Wang,Yong An 한국물리학회 2009 Current Applied Physics Vol.9 No.5
Novel core–double shell particles with poly(methyl methacrylate-co-butyl acrylate) (PMMA-co-BA) as the cores, poly(methyl methacrylate-co-butyl acrylate-co-methacrylic acid) (PMMA-co-BA-co-MAA) as the inner shells, poly(styrene-co-methyl methacrylate) (PS-co-MMA) as the outer shells were prepared by soap-free emulsion polymerization. The acid–alkali osmotic swelling processes were made before the outer shells wrapped for bigger aperture. The optimal experiment conditions were summarized. The morphology and size of the hollow latex particles were observed by transmission electron microscopy. The results showed that the uniform sizes of the hollow latex particles were about 230 nm. The electrophoretic mobility of them in tetrachloroethylene was 0.91 × 10-10 m2 V-1 s-1, and the Zeta-potential was 5.87 mV. The results showed that the hollow polymer particles can used as background particles. Novel core–double shell particles with poly(methyl methacrylate-co-butyl acrylate) (PMMA-co-BA) as the cores, poly(methyl methacrylate-co-butyl acrylate-co-methacrylic acid) (PMMA-co-BA-co-MAA) as the inner shells, poly(styrene-co-methyl methacrylate) (PS-co-MMA) as the outer shells were prepared by soap-free emulsion polymerization. The acid–alkali osmotic swelling processes were made before the outer shells wrapped for bigger aperture. The optimal experiment conditions were summarized. The morphology and size of the hollow latex particles were observed by transmission electron microscopy. The results showed that the uniform sizes of the hollow latex particles were about 230 nm. The electrophoretic mobility of them in tetrachloroethylene was 0.91 × 10-10 m2 V-1 s-1, and the Zeta-potential was 5.87 mV. The results showed that the hollow polymer particles can used as background particles.