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Sheikh Imranudin Sheikh-Ali,Akil Ahmad,Siti-Hamidah Mohd-Setapar,Zainul Akmal Zakaria,Norfahana Abdul-Talib,Aidee Kamal Khamis,Md Enamul Hoque 한국미생물학회 2014 The journal of microbiology Vol.52 No.10
The contamination of food and feed by Aspergillus has becomea global issue with a significant worldwide economicimpact. The growth of Aspergillus is unfavourable to thedevelopment of food and feed industries, where the problemshappen mostly due to the presence of mycotoxins, which is atoxic metabolite secreted by most Aspergillus groups. Moreover,fungi can produce spores that cause diseases, such asallergies and asthma, especially to human beings. High temperature,high moisture, retarded crops, and poor food storageconditions encourage the growth of mold, as well asthe development of mycotoxins. A variety of chemical, biological,and physical strategies have been developed to controlthe production of mycotoxins. A biological approach,using a mixed culture comprised of Saccharomyces cerevisiaeand Lactobacillus rhamnosus resulted in the inhibition of thegrowth of fungi when inoculated into fermented food. Theresults reveal that the mixed culture has a higher potential(37.08%) to inhibit the growth of Aspergillus flavus (producerof Aflatoxin) compared to either single culture, L. rhamnosusNRRL B-442 and S. cerevisiae, which inhibit the growth by63.07% and 64.24%, respectively.
Sheikh, Faheem A,Ju, Hyung Woo,Moon, Bo Mi,Park, Hyun Jung,Kim, Jung Ho,Lee, Ok Joo,Park, Chan Hum Springer 2013 Nanoscale research letters Vol.8 No.1
<P>Electrospinning technique is commonly used to produce micro- and/or nanofibers, which utilizes electrical forces to produce polymeric fibers with diameters ranging from several micrometers down to few nanometers. Desirably, electrospun materials provide highly porous structure and appropriate pore size for initial cell attachment and proliferation and thereby enable the exchange of nutrients. Composite nanofibers consisting of silk and hydroxyapatite nanoparticles (HAp) (NPs) had been considered as an excellent choice due to their efficient biocompatibility and bone-mimicking properties. To prepare these nanofiber composites, it requires the use of acidic solutions which have serious consequences on the nature of both silk and HAp NPs. It is ideal to create these nanofibers using aqueous solutions in which the physicochemical nature of both materials can be retained. However, to create those nanofibers is often difficult to obtain because of the fact that aqueous solutions of silk and HAp NPs can precipitate before they can be ejected into fibers during the electrospinning process. In this work, we had successfully used a three-way stopcock connector to mix the two different solutions, and very shortly, this solution is ejected out to form nanofibers due to electric fields. Different blend ratios consisting HAp NPs had been electrospun into nanofibers. The physicochemical aspects of fabricated nanofiber had been characterized by different state of techniques like that of FE-SEM, EDS, TEM, TEM-EDS, TGA, FT-IR, and XRD. These characterization techniques revealed that HAp NPs can be easily introduced in silk nanofibers using a stopcock connector, and this method favorably preserves the intact nature of silk fibroin and HAp NPs. Moreover, nanofibers obtained by this strategy were tested for cell toxicity and cell attachment studies using NIH 3 T3 fibroblasts which indicated non-toxic behavior and good attachment of cells upon incubation in the presence of nanofibers.</P>
Sheikh, Faheem A.,Barakat, Nasser A. M.,Kanjwal, Muzafar A.,Jeon, Seol-Hee,Kang, Hyung-Sub,Kim, Hak-Yong Wiley Subscription Services, Inc., A Wiley Company 2010 Journal of applied polymer science Vol.115 No.6
<P>In this study, we are introducing a new class of Polyurethane (PU) nanofibers containing silver nanoparticles (NPs) by electrospinning. A simple method not depending on the addition of foreign chemicals has been used to self-synthesize of silver NPs in/on PU nanofibers. Typically, a sol−gel consisting of AgNO<SUB>3</SUB>/PU/N,N-dimethylformamide (DMF) has been electrospun and aged for a week, so silver NPs have been created in/on PU nanofibers. Syntheses of silver NPs were carried out by exploiting the reduction ability of the DMF solvent which is the main constituent to obtain PU electrospun nanofibers in decomposition of silver nitrate precursor into silver NPs. Physiochemical characterizations confirmed well oriented nanofibers and good dispersing of pure silver NPs. Various parameters affecting utilizing of the prepared nanofibers on various nano-biotechnological fields have been studied. For instance, the obtained nanofiber mats were checked for mechanical properties which showed the improvement of the tensile strength upon increase in silver NPs content. Moreover, the nanofibers were subjected to 10 times successive washing experiments with using solid to liquid ratio of 3 : 5000 for 25 h, UV spectroscopy analysis reveals no losses of silver NPs from the PU nanofibers. 3T3-L1 fibroblasts were cultured in presence of the designed nanofibers. The morphological features of the cells attached on nanofibers were examined by BIO-SEM, which showed well attachment of cells to fibrous mats. The cytotoxicity results indicated absence of toxic effect on the 3T3-L1 cells after cell culturing. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2010</P>
The impact of dromedary camel milk on mice gut microbiota
Sheikh Abdullah,Almathen Faisal,Alfattah Mohammed 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.3
The gut microbiota plays an important role in the health and disease resistance of the host. Host health depends on the microbiota of the gastrointestinal tract, and imbalance in its composition may leads to certain diseases. This study analyzed the influence that dromedary camel milk has on the gut microbiota of mice. This study characterized the bacterial populations of untreated [Control (C) and camel milk-treated Raw (R), Pasteurized (P), and Fermented (F)] C57BL/6 J mice feces using high-throughput 16S rDNA sequencing on the Illumina MiSeq platform. In total, 286,606 tags were generated, with an average of 71,651 tags being generated per group, and these tags were clustered to the operational taxonomic units (OTUs) at 97% sequence similarity, resulting 1090 OTUs. Significant weight gain was observed among all of the groups, and the total cholesterol level declined in F group followed by in group P compared to group C. The F and P groups demonstrated a correlation between the beneficial microbiota structures that corresponded with lower cholesterol levels than those observed in the other groups. The major dominant bacteria correspond to the phyla Bacteroidetes and Firmicutes. The dromedary camel milk propagated the beneficial bacteria (Allobaculum and Akkermansia) and reduced harmful bacteria such as Proteobacteria, Erysipelotrichaceae, and Desulfovibrionaceae. This study provides a comparative analysis of the gut microbiota of mice based on camel milk, which may be helpful in understanding host health and diverse gut microbial conditions. The gut microbiota plays an important role in the health and disease resistance of the host. Host health depends on the microbiota of the gastrointestinal tract, and imbalance in its composition may leads to certain diseases. This study analyzed the influence that dromedary camel milk has on the gut microbiota of mice. This study characterized the bacterial populations of untreated [Control (C) and camel milk-treated Raw (R), Pasteurized (P), and Fermented (F)] C57BL/6 J mice feces using high-throughput 16S rDNA sequencing on the Illumina MiSeq platform. In total, 286,606 tags were generated, with an average of 71,651 tags being generated per group, and these tags were clustered to the operational taxonomic units (OTUs) at 97% sequence similarity, resulting 1090 OTUs. Significant weight gain was observed among all of the groups, and the total cholesterol level declined in F group followed by in group P compared to group C. The F and P groups demonstrated a correlation between the beneficial microbiota structures that corresponded with lower cholesterol levels than those observed in the other groups. The major dominant bacteria correspond to the phyla Bacteroidetes and Firmicutes . The dromedary camel milk propagated the beneficial bacteria ( Allobaculum and Akkermansia ) and reduced harmful bacteria such as Proteobacteria , Erysipelotrichaceae, and Desulfovibrionaceae . This study provides a comparative analysis of the gut microbiota of mice based on camel milk, which may be helpful in understanding host health and diverse gut microbial conditions.
Forest Management and Forest Resources in Bangladesh
Sheikh Ali Ahmed,정성호,전수경,김종인 한국가구학회 2007 한국가구학회지 Vol.18 No.2
Total forest land in Bangladesh is about 2.5 million hectare which is 17% of total land area. Bangladeshi forest is mainly classified into three categories- 1) Hill forest 2) Sal forest and 3) Mangroves. This forest land area is decreasing day by day. But in past decayed due to some policies, the total forest land area is increasing slowly. By this planning both government and the local people in the community are being benefited. This paper mainly discussed about the past, present and future trend of forest condition, forest land uses, sustainable forest planning and forest management.