두유 커드를 생산하는 김치 유래 젖산균의 동정

Ro-Ui Kim(김로의),Soon-Cheol Ahn(안순철),Sun-Nyoung Yu(유선녕),Kwang-Youn Kim(김광연),Jong-Hwan Seong(성종환),Young-Guen Lee(이영근),Han-Soo Kim(김한수),Dong-Seob Kim(김동섭) 한국생명과학회 2011 생명과학회지 Vol.21 No.2

본 연구의 목적은 두유 curd를 형성하는 미생물을 분리하는 것이다. 두유 curd를 형성하는 미생물은 채소를 젖산균으로 발효시킨 전통적인 한국의 음식, 김치로부터 분리하였다. 분리 균주 196개 중 10개의 균주(strain No. 2-2-2, 2-15-2, 2-18-1, 2-19-2, 3-4-1, 3-4-2, 3-8-1, 3-8-3, 3-17-1, 4-39-5)가 단단한 두유 curd를 형성하였고 분자생물학적?생화학적 분석법에 의해 동정되었다. 분리균주로부터 추출한 genomic DNA는 16S rDNA 지역의 PCR 증폭을 위한 주형으로 사용하였다. GenBank 데이터로 16S rDNA 염기서열을 비교한 결과, 분리 균주들은 Leuconostoc mesenteroides group과 Lactobacillus sakei group으로 동정되었다. 두유 curd를 형성하는 균주들의 계통 발생학적 위치와 분류군은 neighbor-joining 방법을 이용하여 확인하였다. 또한, L. mesenteroides group은 생화학적 특성에 의해 L. mesenteroides subsp. dextranicum으로 동정되었다. 하지만 L. sakei group은 생화학적 특성 비교시 다양성을 보여 Lactobacillus sp.로 명명하였다. The purpose of this study was to isolate soy curd forming bacterial strains. Soy curd forming bacteria were isolated from Kimchi, a traditional Korean vegetable food that is fermented using lactic acid bacteria. Among 196 bacterial strains, ten isolates (strain No. 2-2-2, 2-15-2, 2-18-1, 2-19-2, 3-4-1, 3-4-2, 3-8-1, 3-8-3, 3-17-1, 4-39-5) formed firm soy curd. The isolated bacterial strains were identified by molecular biological and biochemical analyses. The genomic DNAs extracted from the isolated bacterial strains were used as a template for PCR amplification of 16S rDNA region. By comparing the results of the 16s rDNA sequences with GenBank data, the isolated strains were identified as Leuconostoc mesenteroides group and Lactobacillus sakei group. The phylogenetic position of soy curd forming strains and their related taxa were investigated using neighbor-joining method. L. mesenteroides group was further identified as L. mesenteroides subsp. dextranicum based on biochemical properties. L. sakei group was named Lactobacillus sp., because it showed a variety of biochemical properties.

Transforming Growth Factor β Inhibits MUC5AC Expression by Smad3/HDAC2 Complex Formation and NF-κB Deacetylation at K310 in NCI-H292 Cells

Lee, Su Ui,Kim, Mun-Ock,Kang, Myung-Ji,Oh, Eun Sol,Ro, Hyunju,Lee, Ro Woon,Song, Yu Na,Jung, Sunin,Lee, Jae-Won,Lee, Soo Yun,Bae, Taeyeol,Hong, Sung-Tae,Kim, Tae-Don Korean Society for Molecular and Cellular Biology 2021 Molecules and cells Vol.44 No.1

Airway mucus secretion is an essential innate immune response for host protection. However, overproduction and hypersecretion of mucus, mainly composed of the gel-forming MUC5AC protein, are significant risk factors for patients with asthma and chronic obstructive pulmonary disease (COPD). The transforming growth factor β (TGFβ) signaling pathway negatively regulates MUC5AC expression; however, the underlying molecular mechanism is not fully understood. Here, we showed that TGFβ significantly reduces the expression of MUC5AC mRNA and its protein in NCI-H292 cells, a human mucoepidermoid carcinoma cell line. This reduced MUC5AC expression was restored by a TGFβ receptor inhibitor (SB431542), but not by the inhibition of NF-κB (BAY11-7082 or Triptolide) or PI3K (LY294002) activities. TGFβ-activated Smad3 dose-dependently bound to MUC5AC promoter. Notably, TGFβ-activated Smad3 recruited HDAC2 and facilitated nuclear translocation of HDAC2, thereby inducing the deacetylation of NF-κB at K310, which is essential for a reduction in NF-κB transcriptional activity. Both TGFβ-induced nuclear translocation of Smad3/HDAC2 and deacetylation of NF-κB at K310 were suppressed by a Smad3 inhibitor (SIS3). These results suggest that the TGFβ-activated Smad3/HDAC2 complex is an essential negative regulator for MUC5AC expression and an epigenetic regulator for NF-κB acetylation. Therefore, these results collectively suggest that modulation of the TGFβ1/Smad3/HDAC2/NF-κB pathway axis can be a promising way to improve lung function as a treatment strategy for asthma and COPD.

호스피스 병동과 일반병동의 말기암환자의 간호중재 비교

노유자,한성숙,용진선,송민선,홍진의 성인간호학회 2002 성인간호학회지 Vol.14 No.4

Purpose: The purpose of the study was to compare symptoms, medical therapies, and nursing interventions with terminal cancer patients during the last four weeks of their lives in a hospice unit and general units. Method: For the descriptive survey study, data were collected by reviewing the medical records of 243 patients who died of terminal cancer at K hospital in Seoul. The data was analyzed by using Chi-square test and t-test. Result: The study findings are summarized as follows. There were higher frequencies in physical symptoms of constipation, itching sensation, pain, sleeping disturbance, soreness and dysuria for those patients in the hospice unit than those patient in general units. All emotional symptoms were recorded significantly higher for those patients in the hospice unit than those in general units. Regarding the major medical interventions, pain management was used more significantly for those patients in the hospice unit, but antibiotic therapy and resuscitation were used more significantly for those patients in general units. Conclusion: The hospice unit provided more comprehensive nursing interventions including psychological, spiritual, and family cares as well as physiological care for terminal cancer patients. The facts showed that those patients who would need hospice care in general units should be referred to the hospice unit at an appropriate time.

호스피스 병동과 일반병동의 말기암환자의 간호중재 비교

노유자,한성숙,용진선,송민선,홍진의 가톨릭대학교 간호대학 호스피스 교육연구소 2002 호스피스논집 Vol.6 No.-

The purpose of the study was to compare symptoms, medical therapies, and nursing interventions with terminal cancer patients during the last four weeks of their lives in a hospice unit and general units. For the descriptive survey study, data were collected by reviewing the charts of 243 patients who died of terminal cancer at K hospital in Seoul. The data was analyzed using Chi-square test and t-test. The study findings are summarized as follows: There were higher frequencies in physical symptoms of constipation, itching sensation, pain, sleeping disturbance, sore, and urinary difficulty for those patients in the hospice unit than those patient in general units. All emotional symptoms were recorded significantly higher for those patients in the hospice unit than those in general units. Regarding the major medical treatments, pain control was used significantly more for those patients in the hospice unit but antibiotic therapy and resuscitation were used significantly more for those patients in general units. The hospice unit provided more comprehensive nursing interventions including psychological, spiritual, and family cares as well as physiological care for terminal cancer patients. The facts showed that those patients who need hospice care in general units should be referred to the hospice unite at an appropriate time.

Ryupunghwan (7 Plant Extract Blend) Improves the Destructive Cartilage Through Regulating Proteases and Cytokines Secreted From Mast Cells Infiltrated Into Synovial Membranes in Collagenase II-Induced Osteoarthritis Model in Rats

Hong Gwan Ui,Chung Myung-Hee,Jai Youl Ro 건강기능식품미래포럼 2023 건강기능식품미래포럼 학술지 Vol.3 No.4

Osteoarthritis (OA) is a chronic degenerative disease characterized by synovium inflammation, cartilage destruction and bone margin erosion. Mast cells are multifunctional in the pathogenesis of OA through releasing proteases and inflammatory cytokines when the cells are activated. The seven plants has been reported to have anti-oxidant, anti-inflammatory, and anti-OA effects. Thus, a mixture of seven extracts of respective plants with the adjusted percentage ratio was prepared and named Ryupunghwan (RPH) under the assumption that the mixture has a higher efficacy on OA than the one plant extract. This study was performed to investigate the effect of RPH on OA using a collagenase II (Col II)-induced OA model in rats. To induce OA, Col II was injected in the articular space of right knee of Wistar rats. After 3 days, the rats with OA were orally administered RPH (50, 100, 300 mg/kg daily) for 3 weeks. Joint tissues were stained using Safranin-O for proteoglycan, hematoxylin-eosin for inflammatory cells and May-Grünwald-Giemza stains for mast cells. Synovial fluids were used for assays of amounts of matrix metalloproteinase (MMP) 1, MMP13, and pro-inflammatory cytokines (interleukin [IL]-1β, tumor necrosis factor [TNF]-α, IL-6) by enzyme-linked immunosorbent assay, and MMP2 and MMP9 by gelatin zymography. The OA rats revealed an increase in irregularities in the surface layer of cartilage, proteoglycan loss (red staining), collage destruction by MMP2/9. The OA rats also increased infiltration of inflammatory and mast cells into synovial membranes, amounts of MMP1, MMP9, MMP13, activities of MMP2/9, pro-inflammatory cytokines in synovial fluid, compared to normal rats. However, in OA rats with RPH administered, all these changes indicating cartilage destruction and arthritis were significantly decreased compared to the OA rats. These data suggest that RPH has anti-inflammatory effects on OA through inhibiting the secretion of MMPs and pro-inflammatory cytokines from chondrocytes and mast cells.

Effects of a Mixture of Three Extracts of Wolfberry, Chives and Graviola on the Erectile Dysfunction Induced by Bilateral Cavernous Nerve Injury in Rats

Jai Youl Ro,Gwan Ui Hong,신연호,Chung Myung-Hee 건강기능식품미래포럼 2022 건강기능식품미래포럼 학술지 Vol.2 No.4

Although some chemicals are available for erectile dysfunction (ED), efforts have been also made to seek health functional foods that can improve ED. Lycium barbarum (wolfberry) and Allium tuberosum (chives) were reported to enhance erectile function. And Annona muricata L is known to have anti-inflammatory and antioxidant actions. We assumed that if the three plants were used together, they may enhance erectile function synergistically. Hyunsung Vital Co. Ltd. prepared a mixture of water extracts of the three plants and named it Jikdaijangryeuk (JDJR). In the present study, JDJR was tested for the effect of improving ED in the rats subjected to bilateral corpus cavernous nerve injury (BCNI). BCNI decreased intra-cavernous pressure and also induced biological responses in the penile tissue that lead to ED, which were decreases in the expressions of endothelial nitric oxide (NO) synthase (eNOS) and nervous NO synthase (nNOS) that produce NO (a stimulator of 3′,5′-cyclic guanosine monophosphate [cGMP] synthesis), neurofilament-1 (a marker of nerve fibers) and an anti-apoptotic protein (Bcl2) and in the amounts of NO, cGMP (a blood vessel dilator) and smooth muscle as well as increases in the expression of inducible NO synthase (iNOS) (an inducer of microvasculature dysfunction), phosphodiesterase-5 (a cGMP destroyer), apoptotic molecules (caspase-3 and Bax) and transforming growth factor-β (a fibrosis inducer) and in the amount of asymmetric dimethylarginine (an endogenous NO synthases inhibitor). These data indicate that BCNI suppresses function of NO/cGMP axis and causes apoptosis and fibrosis of cavernous tissue. JDJR, however, reversed all these responses in a dose-dependent manner and the effects of JDJR were comparable to those of sildenafil (a positive control). The powder of Platycodon grandiflorum (a negative control) did not show the effects. These data support that JDJR has the action to enhance erectile function and thus, may be of help for ED.

내부건식기법 호소(湖沼)의 수질개선을 위한 IoT 기반 분석 시스템 구현

김의진(Kim ui-jin),이홍로(Lee hong-ro) 한국콘텐츠학회 2016 한국콘텐츠학회 종합학술대회 논문집 Vol.2016 No.5

Inflammatory mediators resulting from transglutaminase 2 expressed in mast cells contribute to the development of Parkinson's disease in a mouse model

Hong, Gwan Ui,Cho, Jin Whan,Kim, Soo Youl,Shin, Joo Ho,Ro, Jai Youl Elsevier 2018 Toxicology and applied pharmacology Vol.358 No.-

<P><B>Abstract</B></P> <P>This study aimed to investigate the role of transglutaminase 2 (TG2) expressed in mast cells in substantia nigra (SN) in Parkinson's disease (PD) model or human PD patients. C57BL/6 mice received 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) by ip injection to induce PD. Bone marrow-derived mast cells (BMMCs) were adoptively transferred to TG2 knockout (KO or TG2<SUP>−/−</SUP>) mice by iv injection 1 day before MPTP injection or stimulated by 1 methyl-4-phenylpyridinium (MMP<SUP>+</SUP>). KO-MPTP mice showed reduced expression of tyrosine hydroxylase (TH) and dopamine (DA) transporter (DAT) and loss of TH<SUP>+</SUP> DA neurons, and expression of markers (c-kit, tryptase, FcεRI), mediators' release (histamine, leukotrienes, cytokines), and TG2 related to mast cells, and co-localization of DA neuronal cells and mast cells in SN tissues or release of mediators and TG2 activity in SN tissues and sera versus those in WT (wild type)-MPTP or BM + KO-MPTP mice. KO-MPTP mice reversed the alterations of behavior. KO-BMMCs-transferred KO-MPTP (BM + KO-MPTP) mice had restoration of all the responses versus the KO-MPTP mice. MPP<SUP>+</SUP>-stimulated BMMCs had increased mediators' release, which were inhibited by TG2 inhibitor (R2 peptide). All the mediators and TG2 activity were also increased in the sera of human PD patients. The data suggest that TG2 expressed in mast cells recruited into SN tissues might contribute to neuroinflammation, which is known as one of the important features in pathogenesis of PD, via up-regulating the release of various mediators.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Mast cells recruited into substantia nigra (SN) of PD, are activated by MPP<SUP>+</SUP>. </LI> <LI> The activated mast cells activate TG2 in the SN tissues of PD model in mice. </LI> <LI> Mast cells-activated TG2 releases mediators such as histamine, LTs and cytokines. </LI> <LI> Mediators may induce neuroinflammation caused DA neuron death in mouse and human PD. </LI> <LI> TG2 inhibitor may be developed as a therapeutic agent for human PD patients. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Piscroside C inhibits TNF-α/NF-κB pathway by the suppression of PKCδ activity for TNF-RSC formation in human airway epithelial cells

Lee, Su Ui,Lee, Seoghyun,Ro, Hyunju,Choi, Ji-Hee,Ryu, Hyung Won,Kim, Mun-Ock,Yuk, Heung Joo,Lee, Jinhyuk,Hong, Sung-Tae,Oh, Sei-Ryang Elsevier 2018 Phytomedicine Vol.40 No.-

<P>Conclusion: We propose that piscroside C is a promising therapeutic constituent of YPL-001 through its inhibition of PKC delta activity in the TNF-RSC/IKK/NF-kappa B/MUC5AC signaling cascade.</P>

Fisetin inhibits TNF-α/NF-κB-induced IL-8 expression by targeting PKCδ in human airway epithelial cells

Lee, Seoghyun,Ro, Hyunju,In, Hyun Ju,Choi, Ji-Hee,Kim, Mun-Ock,Lee, Jinhyuk,Hong, Sung-Tae,Lee, Su Ui Elsevier 2018 Cytokine Vol.108 No.-

<P><B>Abstract</B></P> <P>Fisetin (3,7,3′,4′-tetrahydroxyflavone), a natural flavonoid, is a therapeutic agent for respiratory inflammatory diseases such as chronic obstructive pulmonary disease (COPD). However, detailed molecular mechanisms regarding the target protein of fisetin remain unknown.</P> <P>Fisetin significantly reduces tumour necrosis factor alpha (TNF-α)-induced interleukin (IL)-8 levels by inhibiting both nuclear factor kappa B (NF-κB) transcriptional activity and the phosphorylation of its upstream effectors. We show that fisetin prevents interactions between protein kinase C (PKC)δ and TNF receptor-associated factor 2 (TRAF2), thereby inhibiting the inhibitor of kappa B kinase (IKK)/NF-κB downstream signalling cascade. Furthermore, we found that fisetin directly binds to PKCδ <I>in vitro</I>. Our findings provide evidence that fisetin inhibits the TNF-α-activated IKK/NF-κB cascade by targeting PKCδ, thereby mediating inflammatory diseases such as COPD.</P> <P>These data suggest that fisetin is a good therapeutic drug for the treatment of inflammatory lung diseases, such as COPD, by inhibiting the TNF-α/NF-κB signalling pathway.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fisetin inhibits TNF-α-increased <I>IL-8</I> gene expression by blocking NF-κB activity. </LI> <LI> Fisetin inhibits TNF-α-mediated interactions between PKCδ and TRAF2. </LI> <LI> Fisetin inhibits PKCδ activity. </LI> </UL> </P>