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Total oxy-radical scavenging capacity assay를 이용한 taurine과 hypotaurine의 항산화 활성 측정
오정민,주홍매,김상겸,김봉희 忠南大學校 生命科學硏究院 醫藥品開發硏究所 2006 藥學論文集 Vol.21 No.-
Taurine, a sulfur containing non-amino acid, is one of the most abundant intracellular amino acid in humans, and hypotaurine is a precursor of the synthesis of taurine. While it is widely believed that taurine may play an important role in protecting cells against toxic injury by functioning as an antioxidant, there is a lack of evidence to support this hypothesis. In this study, total oxy-radical scavenging capacity(TOSC) assay was used to evaluate antioxidant activity of taurine and hypotaurine against peroxyl radical, hydroxyl radical and peroxynitrite. The present results show that taurine does not have oxy-radical scavenging activity and hypotaurine is comparable to glutathione, an antioxidant substance, in oxy-radical scavenging activity. The results suggest that protective effects of taurine against oxidative stress may not be associated with its direct oxy-radical scavenging activity. Difference in oxy-radical scavenging capacity between taurine and hypotaurine may be ascribed to their sulfur oxidation state.
소대화,박정철,이영매,조용준,Korobova, Natalya 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-
For the research of the effects on suspension solution with YBcO and BSCCO for elcectrophoretic deposition to prepare superconducting thick film wire, the preheating technique for the superconducting powders in vaccum system was used with various solvent solutions for electrophoresis such as acetone, toluen and buthanol was investigated. As a result, it was useful to remove the influence of remaining and adsorbed solvent solution which was existed between and on the particle surfaces when the specimens of superconducting wire by electrophoresis were treated in vacuum of 10-3 Torr and temperature around 200℃ in bell-jar system. Of the prepared superconducting wire samples, the critical current density, Jc was measured by 4-point prove method in liquid N₂, and at the value of 10³to 10⁴A/㎠, respectively, for the YBCO and BSCCO superconducting wires.
Clinical relevance for serum cold-inducible RNA-binding protein level in alopecia areata
( Jung-min Shin ),( Jung-woo Ko ),( Xue Mei Li ),( Jin Gwi Yoo ),( Jin-hyup Lee ),( Dong-kyun Hong ),( Chong-won Choi ),( Chang-deok Kim ),( Young-joon Seo ),( Jeung-hoon Lee ),( Young Lee ),( Kyung-d 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2
Background: Alopecia areata (AA), a chronic, relapsing hair-loss disorder, is considered to be a T-cell-mediated autoimmune disease. Cold-inducible RNA-binding protein (CIRP) belongs to a family of cold-shock proteins that respond to cold stress, and has been identified as a damage-associated molecular pattern (DAMP) molecule that triggers the inflammatory response. Recent studies have shown that high-mobility group box 1, another DAMP molecule, is elevated in serum and scalp tissue of AA patients, suggesting a relationship between DAMP molecules and the pathogenesis of AA. Objectives: To investigate the clinical significance of serum CIRP levels in AA. Methods: The serum levels of CIRP were compared between 68 patients with AA and 20 healthy controls. Additionally, the correlation between CIRP level and various clinical parameters was evaluated. Results: The serum CIRP levels were significantly higher in AA patients compared to healthy subjects. Moreover, there was an association between the serum CIRP level and clinical characteristics, such as disease duration and disease activity. However, there was no significant difference in the serum CIRP level among the clinical types of AA (AA multiplex, alopecia totalis, and alopecia universalis) and CIRP expression between normal hair and AA scalp specimens. Conclusion: These results suggest that CIRP may play a significant role in the pathogenesis of AA and could be a potential biologic marker for monitoring the disease activity of AA.
Decreased expression of TFF2 and gastric carcinogenesis
Jung, Kwang-Hwa,Kim, Pum-Joon,Kim, Jeong-Kyu,Noh, Ji-Heon,Bae, Hyun-Jin,Eun, Jung-Woo,Xie, Hong Jian,Shan, Jin Mei,Ping, Win Yin,Park, Won-Sang,Lee, Jung-Young,Nam, Suk-Woo The Korean Society of Toxicogenomics and Toxicopro 2010 Molecular & cellular toxicology Vol.6 No.3
The trefoil factor family (TFF) of peptides, which are protease-resistant and have a strong affinity for mucins, play an important role in gastrointestinal mucosal protection and restitution. Prior studies have indicated that dysregulation of TFF2 was associated with cell migration, resistance to apoptosis, and gastric cancer invasion; however, the underlying mechanism associated with these actions remains unclear. Thus, to investigate the relationship between TFF2 and carcinogenesis in gastric cancer, TFF2 expression was analyzed by Western blot analysis in nine selected gastric cancer tissues and immunohistochemical staining was performed in paraffin-embedded samples from 157 gastric cancers. A reduced TFF2 expression was observed by Western blot analysis in the gastric cancer tissues. However, there was no significant difference in the TFF2 expression according to clinical and pathological parameters of the gastric cancers. To investigate the biological role of TFF2 in the development and progression of gastric cancer, a TFF2 expression plasmid was constructed for in vitro experiments of function. Introduction of TFF2 cDNA into a gastric cancer cell line did not affect tumor cell growth, cell migration or invasion. In conclusion, down-regulation of TFF2 in gastric cancer cells and restoration of TFF2 did not affect the tumorigenic potential of gastric cancer cells in vitro. The loss of TFF2 expression might be an early event of the multi-step process of gastrocarcinogenesis and may play a limited role in the mucosal protection of the normal gastric physiology.
( Jung-min Shin ),( Soo Jung Kim ),( Xue Mei Li ),( Jin Gwi Yoo ),( Dong-kyun Hong ),( Jin-hyup Lee ),( Chong Won Choi ),( Kyung-duck Park ),( Chang Deok Kim ),( Young-joon Seo ),( Jeung-hoon Lee ),( 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2
Background: Alopecia areata (AA) is a chronic, relapsing hair-loss disorder that is considered to be a T-cell-mediated autoimmune disease. Several animal models for AA have been created to investigate the pathophysiology and screen for effective therapeutic targets. Objectives: As C3H/HeJ mice develop AA spontaneously in a low frequency, a novel animal model is needed to establish an AA-like condition faster and more conveniently. In this study, we present a novel non-invasive AA rodent model that avoids skin or lymph-node cell transfer. Methods: We simply injected C3H/HeJ mice subcutaneously with interferon-gamma (IFNγ) along with polyinosinic:polycytidylic acid (poly[I:C]), a synthetic dsRNA, to initiate innate immunity via inflammasome activation. Results: Approximately 80% of the IFNγ and poly(I:C) co-injected mice showed patchy AA lesions after 8 weeks. None of the mice displayed hair loss in the IFNγ or poly(I:C) solely injection group. Immunohistochemical staining of the AA lesions revealed increased infiltration of CD4+ and CD8+ cells infiltration around the hair follicles. IFNγ and poly(I:C) increased the expression of NLRP3, IL-1b, CXCL9, CXCL10, and CXCL11 in mouse skin. Conclusion: Taken together, these findings indicate a shorter and more convenient means of AA animal model induction and demonstrate that inflammasome-activated innate immunity is important in AA pathogenesis.
Min Jung Ko(고민정),Seetharaman Rajasekar(라자세커 시타르만),Ziyu Wang(왕자옥),Mei Li(이매),Jung Ho Kwak(곽정호),Young Hoon Park(박영훈),Beung Gu Son(손병구),Jum Soon Kang(강점순),Young Whan Choi(최영환) 한국생명과학회 2016 생명과학회지 Vol.26 No.2
암전이는 현재까지 적당한 치료제가 거의 없었기 때문에 암에 의한 사망의 주요한 원인 중의 하나로 인식되고 있다. 최근 본 연구팀은 마늘 추출물과 순수분리한 성분에 대한 암전이 억제 시험 결과 마늘의 추출물 또는 성분이 암전이를 억제시켰으며, 역학조사에서도 마늘을 많이 섭취한 사람은 암의 발생을 억제시키는 것으로 보고되어 있다, 본 연구의 암전이 실험에서는 C57BL/6 mouse의 꼬리 정맥에 melanoma B16F10세포를 주사하여 폐에 전이를 유도하였다. 암세포 주사 1일 후에 마늘의 헥산 추출물 50, 100 및 200 mg/kg body weight를 2일 간격으로 21일 동안 구강투여 한 다음 암전이 억제효과를 조사하였다. GHE를 처리하지 않은 대조구에서는 폐에서 암 colony가 97.4±30.2으로 대량 생성되었다. GHE를 50, 100 및 200 mg/kg의 농도로 경구투여시에 암전이 빈도는 각각 6.93, 46.80 및 50.53% 억제하였다. 또한 100 mg/kg body weight 경구투여 시에는 폐로 암전이 억제율이 약 53% 이상으로 매우 높았다. 폐에서 melanoma cell colony의 발생율과 면적은 마늘 헥산 추출물의 농도가 높을수록 감소하였다. 결론적으로 C57BL/6 mice의 암전이 모델에서 마늘 헥산추출물의 구강투여는 폐에 암전이를 억제시켰으나, 향후 그 기작에 대한 연구가 수행되어야 할 것으로 생각된다. Metastatic cancer is one of the main causes of cancer-related death since they rarely respond to available treatments. There is epidemiologic evidence that high garlic consumption decreases the incidence of cancer. Recent studies of our laboratory have revealed that a garlic-extracts is effective in suppressing metastasis. For experimental metastasis, C57BL/6 mice were injected intravenously with melanoma B16F10 cells in the tail vein, and were orally administered various concentrations (0, 50, 100 or 200 mg/kg body weight) of garlic hexane extract (GHE) for 21 days. The incidence and the area of the melanoma cell colony occupied by the poorly differentiated carcinoma were significantly lower in dose-dependent in 50, 100 and 200 mg/kg BW GHE–treated mice compared with control mice. In conclusion, the results of the present study show that GHE administration prevents lung metastasis in C57BL/6 mice.