A Synthetic Naringenin Derivative, 5-Hydroxy-7,4′-diacetyloxyflavanone-<i>N</i>-phenyl Hydrazone (N101-43), Induces Apoptosis through Up-regulation of Fas/FasL Expression and Inhibition of PI3K/Akt Signaling Pathways in Non-Small-Cell Lung Cancer Cells

Bak, Yesol,Kim, Heejong,Kang, Jeong-Woo,Lee, Dong Hun,Kim, Man Sub,Park, Yun Sun,Kim, Jung-Hee,Jung, Kang-Yeoun,Lim, Yoongho,Hong, Jintae,Yoon, Do-Young American Chemical Society 2011 Journal of agricultural and food chemistry Vol.59 No.18

<P>Naringenin, a well-known naturally occurring flavonone, demonstrates cytotoxicity in a variety of human cancer cell lines; its inhibitory effects on tumor growth have spurred interest in its therapeutic application. In this study, naringenin was derivatized to produce more effective small-molecule inhibitors of cancer cell proliferation, and the anticancer effects of its derivative, 5-hydroxy-7,4′-diacetyloxyflavanone-<I>N</I>-phenyl hydrazone (N101-43), in non-small-cell lung cancer (NSCLC) cell lines NCI-H460, A549, and NCI-H1299 were investigated. Naringenin itself possesses no cytotoxicity against lung cancer cells. In contrast, N101-43 inhibits proliferation of both NCI-H460 and A549 cell lines; this capacity is lost in p53-lacking NCI-H1299 cells. N101-43 induces apoptosis via sub-G<SUB>1</SUB> cell-cycle arrest in NCI-H460 and via G<SUB>0</SUB>/G<SUB>1</SUB> arrest in A549 cells. Expression of apoptosis and cell-cycle regulatory factors is altered: Cyclins A and D1 and phospho-pRb are down-regulated, but expression of CDK inhibitors such as p21, p27, and p53 is enhanced by N101-43 treatment; N101-43 also increases expression levels of the extrinsic death receptor Fas and its binding partner FasL. Furthermore, N101-43 treatment diminishes levels of cell survival factors such as PI3K and p-Akt dose-dependently, and N101-43 additionally induces cleavage of the pro-apoptotic factors caspase-3, caspase-8, and poly ADP-ribose polymerase (PARP). Cumulatively, these investigations show that the naringenin derivative N101-43 induces apoptosis via up-regulation of Fas/FasL expression, activation of caspase cascades, and inhibition of PI3K/Akt survival signaling pathways in NCI-H460 and A549 cells. In conclusion, these data indicate that N101-43 may have potential as an anticancer agent in NSCLC.</P>

2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242) induces apoptosis via modulating E7 expression and inhibition of PI3K/Akt pathway in SiHa human cervical cancer cells.

Kim, Man Sub,Kim, Jung Hee,Bak, Yesol,Park, Yun Sun,Lee, Dong Hun,Kang, Jeong Woo,Shim, Jung-Hyun,Jeong, Heon Sang,Hong, Jin Tae,Yoon, Do Young Lawrence Erlbaum Associates, Publishers [etc.] 2012 Nutrition and cancer Vol.64 No.8

<P>The Maillard reaction is a chemical reaction occurring between an amino acid and a reducing sugar, usually requiring thermal processing. Maillard reaction products (MRPs) have antioxidant, antimutagenic, and antibacterial effects, and although 2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242), a fructose-tyrosine MRP, appears to inhibit proliferation of cancer cells, its mechanism of action has not been studied in detail. We found that HPB242 treatment modulated expression of cyclins and tumor suppressor genes in SiHa human cervical cancer cell lines: cyclins and phospho-pRB were downregulated, whereas the expression of CDK inhibitors and p53 was enhanced. HPB242 induced apoptosis dose-dependently by suppressing E7 expression and leading to sub-G1 cell-cycle arrest in SiHa cell lines; treatment also led to the proteolytic cleavage of caspase-3, -9, and poly (ADP-ribose) polymerase. Moreover, HPB242 upregulated Fas expression, altered expressions of pro- and antiapoptotic factors, and also inhibited nuclear translocation of nuclear factor κB and phosphorylation of IκB. HPB242 treatment decreased phosphatidyl inositol-3 kinase and p-Akt expression levels, demonstrating that this survival pathway may also be inhibited by HPB242. Cumulatively, HPB242 promotes apoptosis by influencing E7 expression, inducing cell-cycle arrest at sub-G1 phase, and promoting both intrinsic (mitochondrial) and extrinsic (Fas-dependent) apoptosis in SiHa human cervical cancer cells.</P>

The Biflavonoid Amentoflavone Induces Apoptosis via Suppressing E7 Expression, Cell Cycle Arrest at Sub-G1 Phase, and Mitochondria-Emanated Intrinsic Pathways in Human Cervical Cancer Cells

Sojung Lee,Heejong Kim,Jung-Hee Kim,이동훈,Man-Sub Kim,양영,우은란,김양미,홍진태,강정우,윤도영 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.7

Amentoflavone, a biflavonoid from Selaginella tamariscina, is known to possess several bioactivities such as antitumor, anti-inflammatory, and antifungal effects. However, the mechanism of the anticancer effects of amentoflavone on human cervical cancer cells has not been studied in detail. In this study, we demonstrated that amentoflavone induces apoptosis in SiHa and CaSki cervical cancer cells by suppressing human papillomavirus protein E7 expression. The cyclins and tumor suppressors were modulated by amentoflavone in SiHa and CaSki human cervical cancer cells: cyclin and hyperphosphorylated retinoblastoma (p-pRb) were down-regulated, whereas cyclin-dependent kinase inhibitors and p53 were enhanced. Amentoflavone up-regulated peroxisome proliferator-activated receptor γ (PPARγ) and phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression levels while inhibiting E7-mediated cyclooxygenase-2 (COX-2)/interleukin-32 (IL-32) expressions were downregulated, and Akt phosphorlylation was decreased in an amentoflavone-induced apoptotic process, suggesting that amentoflavone may be a PPARγ activator. Additionally, the expression of the anti-apoptotic factor Bcl-2 was decreased, whereas that of the well-known apoptotic factor Bax was increased, thereby releasing cytochrome c into cytosol in amentoflavone-treated cervical cancer cells. Furthermore, amentoflavone treatment led to the activation of caspase-3 and -9 and proteolytic cleavage of poly(ADP-ribose) polymerase. The expression level of the extrinsic death receptor Fas (CD95) was not altered by amentoflavone treatment. When these findings are taken together, the biflavonoid amentoflavone activates PPARγ/PTEN expressions and induces apoptosis via suppressing E7 expression, cell cycle arrest at sub-G1 phase, and mitochondria-emanated intrinsic pathways in SiHa and CaSki human cervical cancer cells. These findings suggest that amentoflavone has potential for development as a therapeutic agent for human cervical cancer.

분광학적 방법에 의한 이소니트로소벤조일아세톤의 코발트(Ⅲ) 및 팔라듐(Ⅱ) 착물의 구조

吳岱燮,李萬浩,金修漢 慶北大學校 1979 論文集 Vol.27 No.-

Isonitrosobenzoylacetone is particularly interesting, as the isonitroso group has two potentially coordinating sites which can compete with the carbonyl groups in forming bonds with the metal ions. In this paper tris(isonitrosobenzoylacetonato)cobalt(III) and bis(isonitrosobenzoylacetonato) palladium(II) have been prepared, and their structures have been investigated. Spectroscopic studies lead to the conclusion that the both complexes do not contain an OH group in the chelated five-membered ring structure in which the ligand coordinates to metal through oxygen of the acetyl group and nitrogen of the isonitroso group. The coordination manner of this ligand is similar to that of isonitrosoacetylacetone obtained by Haldar et al.^(3)(4) Very weak intramolecular hydrogen bonding of the free ligand is indicated by nmr and ir spectra.

아연전해스라임으로부터 이산화망간의 회수 및 정제

吳岱燮,李萬浩,金昌福 慶北大學校 1985 論文集 Vol.39 No.-

Manganese slimes from zinc refinery electrolytic cell contain 59.94% MnO_2. The recovery process of MnO_2 from the slimes consists essentially of five steps, i.e., roasting of the slimes, dissolution of the manganese, removal of iron, electrolytic synthesis on MnO_2 from MnSO_4 solution, and preparation of finished MnO_2. Also we report here the optimum conditions of the recovery process of MnO_2 from mangaanese slimes. The crystal structure of MnO_2 product is γ-type.

후천성 이중 유문 1예

정규성,조기섭,정재용,조영호,민영돈,박찬국,김만우 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.1

Double pyrolus, one of the relatively rare anomalies of the gastrointestinal tract, is a fistulous communication between the gastric antrum and the duodenal bulb. The first case of a double Pylorus was reported in 1969 by Smith, since then several cases have been reported. Recently, as the frequency of examining upper gastrointestinal series and endoscopy increases, it occurs more frequently and a few cases have been described in our country. According to the reports previously published, it resulted only from cysts, diverticla, atresia, band, membrane malformation of the pylorus, however, it might be also caused by penetration, complication of peptic ulcer. We report a case of double pylorus in a 67-year old man with prolonged administration of corticostetoids due to lumbago who has complained epigastric pain which was confirmed by endoscopy and biopsy.

RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단

배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.

Self-assembled porous MoO₂/graphene microspheres towards high performance anodes for lithium ion batteries

Palanisamy, Kowsalya,Kim, Yunok,Kim, Hansu,Kim, Ji Man,Yoon, Won-Sub Elsevier 2015 Journal of Power Sources Vol.275 No.-

<P><B>Abstract</B></P> <P>Three dimensional (3D) porous self-assembled MoO<SUB>2</SUB>/graphene microspheres are successfully synthesized via microwave-assisted hydrothermal process in a short reaction time followed by thermal annealing. Such rationally designed multifunctional hybrid nanostructure is constructed from interconnected MoO<SUB>2</SUB> nanoparticles (3–5 nm), which is self-assembled into ordered nanoporous microspheres via strong electrostatic attraction between graphene sheets and MoO<SUB>2</SUB> nanoparticles. The MoO<SUB>2</SUB>/graphene hybrid structure delivers a high reversible capacity with significantly enhanced cycling stability (∼1300 mAh g<SUP>−1</SUP> after 80 cycles at C/10 rate) and excellent rate capability (913 and 390 mAh g<SUP>−1</SUP> at 2C and 5C rates, respectively), when used as an anode material. The microspheres are interconnected and well encapsulated by the flexible graphene sheets, which not only accommodates large volume change but also increases the electrical conductivity of the hybrid structure. Moreover, nanoporous voids present in the 3D framework facilitate effective electrolyte penetration and make a direct contact with the active MoO<SUB>2</SUB> nanoparticles, thereby greatly enhancing lithium ion transport. The strategic combination of self-assembly, nanoporous voids, 3D network and intriguing properties of graphene sheets provides excellent electrochemical performance as anode materials for Lithium ion battery applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> MoO<SUB>2</SUB>/graphene microsphere is synthesized by microwave assisted hydrothermal method. </LI> <LI> Graphene encapsulation driven the self-assembly of 3D porous framework. </LI> <LI> Nanoporous voids enable effective electrolyte penetration. </LI> <LI> Strategic combination of 3D architecture provides superior Li ion storage property. </LI> </UL> </P>

Enhancement of the interfacial reaction on mesoporous RuO₂ for next generation Li batteries

Kim, Yunok,Yoon, Jeasang,Park, Gwi Ok,Park, Su Bin,Kim, Hyunchul,Kim, Ji Man,Yoon, Won-Sub Elsevier 2018 Journal of Power Sources Vol.396 No.-

<P><B>Abstract</B></P> <P>In order to develop high energy density Li rechargeable batteries, nano-sized materials have attracted attention as the active materials. Mesoporous materials consist of the micrometre-sized particles, and they have high surface area due to their mesopore and thin wall thickness of framework. Here, we synthesize a highly ordered mesoporous RuO<SUB>2</SUB> to investigate the effect that the mesoscopic structure has on the capacity and their corresponding reaction mechanism of Li rechargeable battery. The synthesized mesoporous RuO<SUB>2</SUB> shows an initial discharge capacity of 1366 mAh g<SUP>−1</SUP> on mesoporous RuO<SUB>2</SUB>, which is higher than that of commercial RuO<SUB>2</SUB>. Our findings <I>via In situ</I> X-ray analysis techniques combined with electrochemical analysis demonstrate that the additional capacity of mesoporous RuO<SUB>2</SUB> is resulted from the enhanced interfacial reaction between Ru metal and Li<SUB>2</SUB>O formed by conversion reaction of RuO<SUB>2</SUB>. Nano-size effects of mesoporous structure such as high surface area, easy electron transport, and small domain would enable to improve the interfacial reaction of highly ordered mesoporous RuO<SUB>2</SUB>. The understanding of this relationship between structural engineering and electrochemical properties provides the insight into development of high energy density anode materials in next generation Li rechargeable battery.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Mesoporous RuO<SUB>2</SUB> shows higher discharge capacity than that of commercial RuO<SUB>2</SUB>. </LI> <LI> The larger capacity of mesoporous RuO<SUB>2</SUB> is due to the enhanced interfacial reaction. </LI> <LI> Nano-size effects enable to improve the interfacial reaction. </LI> </UL> </P>

New Insight into the Reaction Mechanism for Exceptional Capacity of Ordered Mesoporous SnO₂ Electrodes via Synchrotron-Based X-ray Analysis

Kim, Hyunchul,Park, Gwi Ok,Kim, Yunok,Muhammad, Shoaib,Yoo, Jaeseung,Balasubramanian, Mahalingam,Cho, Yong-Hun,Kim, Min-Gyu,Lee, Byungju,Kang, Kisuk,Kim, Hansu,Kim, Ji Man,Yoon, Won-Sub American Chemical Society 2014 Chemistry of materials Vol.26 No.22

<P>Tin oxide-based materials, operating via irreversible conversion and reversible alloying reaction, are promising lithium storage materials due to their higher capacity. Recent studies reported that nanostructured SnO<SUB>2</SUB> anode provides higher capacity beyond theoretical capacity based on the alloying reaction mechanism; however, their exact mechanism remains still unclear. Here, we report the detailed lithium storage mechanism of an ordered mesoporous SnO<SUB>2</SUB> electrode material. Synchrotron X-ray diffraction and absorption spectroscopy reveal that some portion of Li<SUB>2</SUB>O decomposes upon delithiation and the resulting oxygen reacts with Sn to form the SnO<SUB><I>x</I></SUB> phase along with dealloying of Li<SUB><I>x</I></SUB>Sn, which are the main reasons for unexpected high capacity of an ordered mesoporous SnO<SUB>2</SUB> material. This finding will not only be helpful in a more complete understanding of the reaction mechanism of Sn-based oxide anode materials but also will offer valuable guidance for developing new anode materials with abnormal high capacity for next generation rechargeable batteries.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/cmatex/2014/cmatex.2014.26.issue-22/cm5025603/production/images/medium/cm-2014-025603_0012.gif'></P>