사람 난포액의 처리 방법과 Sample이 생쥐 수정란의 체외 발달율에 미치는 영향

전병균,최연희,조은정,송건호,곽대오,문진수,김광철,Jeon, Byeong-Gyun,Choi, Yeon-Hee,Jo, Eun-Jung,Song, Gun-Ho,Kwak, Dae-Oh,Moon, Jin-Soo,Kim, Kwang-Chul 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.4

The present studt was performed to investigate the effect of treatment and samples of human follicular fluid (hFF) on the development in vitro of mouse embryos. The two cell stage embryos collected at 40 h post-hCG injection were cultured in the modified human tubal fluid (m-RTF) containing 15% synthetic serum substitute (SSS) or human tubal fluid (hFF) for up to 3 days at $37^{\circ}C$ in 5% $CO_2$ incubator. Also the composition of hormone, total protein and protein pattern of hFF samples were analyzed. The developmental rate of mouse embryos developed to blastocyst were not significant difference in the m-RTF containing 15% hFF filtered with 0.22 or 0.8 ${\mu}m$ syringe filter, however, the embryos cultured in the m-RTF containing inactivated hFF were significantly (p<0.05) developed at the high rate to blastocyst than those containing fresh hFF and SSS. The in vitro developmental rate to blastocyst and hatched blastocyst in the m-RTF containing 15% hFF sample A (90.5 and 85.4%, respectively) and SSS (79.4 and 75.3, respectively) were significantly (p<0.05) increased, compared with hFF sample B (64.2 and 54.1 %, respectively). The hFF sample A tended to be higher concentration of LH, FSR, total protein and the ratio of progesterone/$E_2$ and lower concentration of $E_2$ and progesterone than the hFF sample B, but there were no differences in the protein pattern between the two hFF samples. The results of these study suggest that the addition of hFF to the culture medium enhances the development in vitro to blastocyst and hatched blastocyst, but the in vitro developmental rate of mouse embryos is different between hFF samples.

지방세포로의 분화를 통한 악성 종양의 치료 가능성

전병균(Byeong-Gyun Jeon),이성호(Sung-Ho Lee) 한국생명과학회 2023 생명과학회지 Vol.33 No.6

반복핵이식에 의한 복제동물 생산에 관한 연구 III. 토끼에서 제3세대 복제수정란의 생산

이효종,전병균,윤희준,박충생,최상용,윤창현,강대진,Lee Hyo-jong,Jeon Byeong-gyun,Yin Xi-jun,Park Choong-saeng,Choe Sang-yong,Yun Chang-hyun,Kang Dae-jin 한국임상수의학회 1995 한국임상수의학회지 Vol.12 No.1

The recycling nuclear transplantation(NT) technique has the powerful potential of producing a large number of genetically identical embryos and offsprings from one embryo. Multiple generational cloning by this technique utilizes the NT embryo itself as the donor for the next generation of cloning. In this experiment, we have produced the third generational cloned embryos by recycling NT. Further we examined comparatively the electrofusion rate and in vitro developmental potential in the cloned embryos of the first second and third generations. The embryos of 16-cell stage were collected from the mated does by flushing oviducts with Dulberco's phosphate buffered saline containing 10 % fetal calf serum(FCS) at 47 hours after hCG injection. In the first generation NT, the nuclear donor embryos were synchronized in the phase of Gl/S transition of 32-cell stage. The first and second generation NT embryos developed to 16-cell were used as donor nuclei for second and third generation. The recipient cytoplasms were utilized the oocytes collected at 14 hours after hCG injection, following revoming the nucleus and the first polar body by micromanipulation. The separated blastomeres were injected into the enucleated recipient oocytes by micromanipulation and were fused by electrical stimulation. The electrofusion rate was seen to be 78.0, 88.0 and 90.3 % in the first second and third generation NT rabbit embryos, respectively. The fused oocytes were co-cultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10 % FCS for 120 hours at 39$^{\circ}C$ in a 5% $CO_2$ incubator. The in vitro developmental potential to blastocyst stage was significantly(P<0.05) decreased in the third(7.2 %) generation NT embryos compared to the first(53.1 %) and second(16.1 %) generation NT embryos. Following in vitro development to blastocyst stage, they were stained with Hoechst 33342 dye for counting the number of blastomeres by fluorescence microscopy. The mean blastomere numbers and cell cycle numbers of NT embryos during the culture period were significantly(p<0.05) decreased in the second(93.9 cells and 6.55 cylces) and third(81.5 cells and 1.35 cylces) generation, compared to the first(189.9 cells and 7.55 cylces) generation.

ICSI 프로그램에서 생쥐 투명대를 이용한 고환조직내 정자의 동결

서태광,전병균,류은경,이은숙,류재웅,손시환,문진수,김광철,Suh, Tae-Kwang,Jeon, Byeong-Gyun,Ryu, Eun-Kyung,Lee, Eun-Sook,Ryoo, Zae-Yoong,Sohn, Sea-Hwan,Moon, Jin-Soo,Kim, Kwang-Chull 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.2

The survival rate and motility recovered after cryopreservation of testicular spermatozoa in testicular sperm extraction (TESE)-ICSI program is low. The purpose of this study was to assess the availability and efficiency of mouse empty zona pellucida in cryopreserving human TESE spermatozoa. Mouse empty zonae pellucidae were obtained by extraction of cytoplasm with or without cytochalasin B treatment. Motile sperm from proven-fertile donor and two azoospermic patients after TESE were individually inserted into empty zona pellucida and cryopreserved. Two to five days after cyropreservation, the frozen sperm were thawed and the rates of recovery and motility were observed. The ooplasmic extraction rates of control (N=80) and cytochalasin B treated oocytes (N=80) were 94.0% and 96.2%, respectively (p>0.05). The post-thaw recovery rates of spermatozoa and rates of motility recovery of ejaculate (N=70) and testicular (N=70) sperm were 97.1%, 97.1% and 95.7%, 94.3%, respectively (p>0.05). The results of this study showed that the mouse zone pellucida is useful for cryostorage of single testicular spermatozoa.

양파 수확 후 잔재물과 쇠비름 추출물이 유기농 양파의 수확량 및 품질 특성에 미치는 영향

김태원(Tae-Won Kim),전병균(Byeong-Gyun Jeon),이성호(Sung-Ho Lee) 한국생명과학회 2017 생명과학회지 Vol.27 No.12

본 연구는 수확 후 버려지는 양파 잔재물과 쇠비름으로부터 추출한 수액을 유기농 양파 재배 시 시비하여 유기농 양파의 수확량, 품질 등에 미치는 영향을 평가하였다. 도복기 때의 양파 생육은 초장, 엽수, 엽초경에서 식물추출액 처리구들이 무처리구보다 좋았다. 양파 수확량도 식물 추출액 처리구들이 무처리구보다 유의성 있게 증가되었으며(p<0.05), 구 중 분포에서는 상품성이 있는 300 g 이상의 대구 양파가 양파와 쇠비름 추출액 혼용 처리구에서 다른 처리구들보다 유의성 있게 증가 되었다(p<0.05). 양파의 무기성분 함량은 양파와 쇠비름 추출액 혼용처리구에서 생산된 양파가 다른 처리구들에서 생산된 양파보다 석회와 황을 제외한 다량원소들이 높게 함유되었다. 양파의 경도는 양파와 쇠비름 추출액 혼용 처리구에서 생산된 양파가 양파 잔재물 추출액 처리구와 무처리구에서 생산된 양파보다 각각 8%와 20% 높게 나와 유의성을 나타내었지만(p<0.05), 당도는 모든 처리구에서 생산된 양파가 비슷하게 나와 유의성을 나타내지 않았다. 양파 수확 후 토양의 무기성분 함량은 양파 잔재물 추출액 처리구 토양에서 인산, 칼슘, 황산, 망간에서 높게 나타났다. 이상의 결과로 양파 수확 후 버려지는 잔재물과 길가에 방치되는 쇠비름의 추출물이 유기 양파 재배 시 천연 친환경 자재로 활용 가치가 있다고 보겠다. This study assessed the effects of treatment with sap extract from onion residues postharvest and purslane on the quality and quantity of organic onions. At the bending stage, onions treated with the sap extract showed vigorous growth, with higher plant heights, more leaves, and longer sheath lengths than untreated onions. The onion yield was significantly increased when the plant was treated with extracted sap as compared with that of untreated plants (p<0.05). The bulb weight distribution of onions in the mixed onion and purslane treatment was also significantly increased (~300 g) as compared with that of the other treatment (p<0.05). Except for CaO and S, the mineral content of the onions produced from plants treated with the onion and purslane extract mixture was higher than those of onions in the other treatment. The hardness of onions produced from plants treated with the onion and purslane extract was significantly increased (8% and 20%, respectively) as compared with that of onions produced from plants treated with the onion extract only or no treatment (p<0.05). However, there was no significant difference in the sugar contents of the onions produced from extract-treated and nonextract-treated plants. Postharvest, the content of inorganic components (phosphate, calcium, sulfuric acid, and manganese) was higher in soil treated with the onion extract than in soil treated with the onion and purslane extract and non-treated soil. It can be concluded that residues left after onion harvests and purslane extract can be used as natural and environmentally friendly materials for the cultivation of organic onions.

사람의 암세포와 정상세포에서 말단소립의 길이 및 말단소립복원효소의 활성도 비교 분석

이현주 ( Hyeon-joo Lee ),전병균 ( Byeong-gyun Jeon ) 경상대학교 중등교육연구소 2018 현대교육연구 Vol.30 No.2

말단소립(telomere)과 말단소립복원효소(telomerase)는 암세포의 무한 증식에 가장 중요한 인자이다. 이 연구에서는 말단소립의 길이와 말단소립복원효소의 활성도를 여러 암세포에서 정상세포와 비교 분석하였다. 현재의 연구에 사용된 암세포주는 폐암세포(A-549), 유방암세포(MDA-MB-231), 악성 신경교종세포(U87-MG), 췌장암세포(PANC-1) 및 유방암세포(MCF-7)이었고, 정상세포주는 섬유아세포(MRC-5) 및 사랑니 유래 치아줄기세포(DPSC)이었다. Southern blotting으로 분석된 말단소립의 길이는 정상세포주(MRC-5 및 DPSC)에서 여러 암세포주(A-549, MDA-MB-231, U87-MG, PANC-1 및 MCF-7)에 비하여 유의적으로(P<0.05) 길었다. 그러나 여러 암세포주(A-549, MDA-MB-231, U87-MG, PANC-1 및 MCF-7)의 말단소립복원효소의 활성도는 거의 정상세포주(MRC-5 및 DPSC)에 비하여 대략적으로 8배 정도 현저히 증가하는 것으로 나타났다. 따라서 세포분열이 진행될수록 암세포주의 말단소립 길이는 그대로 유지되어 무한 증식을 하지만, 정상세포주의 말단소립 길이는 점점 소실되어 정상세포의 말단소립 길이는 암세포의 말단소립 길이보다 짧아져 세포 사멸에 이를 것이라고 판단된다. 또한, 말단소립복원효소는 주로 telomerase reverse transcriptase (TERT) 단백질과 telomerase RNA component (TERC)로 구성되는데, 말단소립복원효소 활성이 높게 나타나는 암세포주에서 TERC 전사체보다 TERT의 발현이 말단소립복원효소의 활성에 더 큰 영향을 주는 요인임을 알 수 있었다. The telomere and telomerase activity are thought to be a main regulation for immortalization of cancer cells. The present study compared the telomere length and telomerase activity in human cancer (A-549, MDA-MB-231, U87-MG, PANC-1, and MCF-7) and normal (MRC-5, and DPSC) cell lines. The telomere length by southern blotting was significantly (P<0.05) higher in MRC-5 and DPSC than in A-549, MDA-MB-231, U87-MG, PANC-1 and MCF-7 cancer cell lines. However, the relative telomerase activity by RQ-TRAP was significantly (P<0.05) higher in A-549, MDA-MB-231, U87-MG, PANC-1 and MCF-7 cancer cell lines than that of MRC-5 and DPSC. Further, the expression level of TERT and TERC transcripts were quantified by RT-PCR anaysis, and the expression level of TERT transcripts was significantly (P<0.05) increased in A-549, MDA-MB-231, U87-MG, PANC-1 and MCF-7 cancer cell lines with high telomerase activity, compared to that of MRC-5 and DPSC cell lines. Therefore, we conclude that cancer cells possessed short telomere length, but high telomerase activity by up-regulated TERT expression.

DNA 복제 시점의 차이를 응용한 불활성화 X-염색체에 대한 학습 자료의 모델

문선하 ( Moon Sun-ha ),전병균 ( Jeon Byeong-gyun ) 경상대학교 교육연구원 2023 현대교육연구 Vol.35 No.1

XY 성 결정 체계에 의해 암수가 결정되는 포유류의 경우, 두 X-염색체를 가지는 암컷에서 두 X-염색체 중의 하나는 불활성화되고, 이에 따른 여러 가지 성과 관련된 유전 현상이 나타난다. 이러한 X-염색체의 불활성화 및 후성학적인 유전자의 발현 등에 대한 개념을 을 좀 더 쉽게 이해할 수 있는 학습 자료 및 모델이 고등학교 교과서에서 잘 제시되지 않고 있다. 이에 이 연구에서는 DNA 복제 시점의 차이를 이용한 BrDu 표지 방법으로 두 X-염색체의 활성 및 불활성 상태를 좀 더 쉽게 구별하고, 이를 고등학교 생명과학 교과서에 응용할 수 있는 수업 자료를 개발하고자 하였다. 암컷의 섬유아세포를 사용하여 세포 주기를 동기화시킨 다음, S기의 초기 단계에 BrDu 표지를 실시하여, 핵형분석을 통해 X-염색체를 관찰하였다. 이에 S기의 초기에 복제되는 활성 X-염색체와 후반기에 복제되는 불활성 X-염색체를 명확하게 구분할 수 있었다. 또한, 불활성된 X-염색체의 탈메틸화는 후반기에서 전반기 단계로 DNA 복제 시점의 변화가 일어나는 것을 관찰하였다. 이 연구의 결과를 바탕으로 고등학교 생명과학 분야에서 염색체, 유전체, DNA, 유전자의 관계를 이해하고, 후성유전학 및 X-염색체 불활성화 개념을 가시적으로 이해할 수 있는 자료에 응용할 수 있을 것으로 판단된다. The XY chromosome is a main sex-determination system in most mammals. One of the two X-chromosomes is randomly inactivated in the female with two X-chromosomes, and the various sex-linked genetic and epigenetic expression are accompanied by the inactivated X-chromosome. However, the effective teaching model and materials for inactivated X-chromosome that students easily understandable are not presented in the high school life science textbook. The present study is to develop teaching model for application of inactivated X-chromosome with DNA replication assay using BrDu labeling. The female bovine fibroblasts were synchronized at G1 stage of cell cycle and labeled with BrDu. The fibroblasts were again treated with colcemid for synchronization of metaphase. Following BrDu labeling, the status of activated and inactivated X-chromosome was easily distinguished on the karyotyping, and DNA demethylation with SAH treatment was induced to activated X-chromosome from inactivated X-chromosome by change of DNA replication timing. Taken together, the present results have showed that learning model for inactivated X-chromosome and epigenetics can be applied by DNA replication timing assay in the high school life science textbook.

고추 수확 후 잔재물 추출물의 생리활성과 세포독성 분석

김태원(Tae-Won Kim),전병균(Byeong-Gyun Jeon),이성호(Sung-Ho Lee) 한국생명과학회 2020 생명과학회지 Vol.30 No.12

본 연구에서는 고추(Capsicum annuum L.) 수확 후 버려지는 잔재물의 활용가치를 위해 이들로부터 추출한 발효추출물과 열수추출물의 생리활성과 세포독성을 분석하였다. 녹광고추 발효추출물에 함유된 질소, 인산, 칼륨, 칼슘, 마그네슘 성분의 총 함량은 청양고추 발효추출물 보다 2배 정도 높았고, 열수추출물에서는 유사한 함량을 보였다. 미량원소 중에는 붕산, 철, 규소 성분만 검출되고, 아연, 망간, 몰리브덴, 구리 성분은 두 추출물 모두에서 검출되지 않았다. 총 폴리페놀과 플라보노이드 함량은 청양고추와 녹광고추 모두에서 발효추출물이 열수추출물 보다 2배 이상 높게 나타내었다. DPPH radical 소거 능력은 발효추출물이 열수추출물 보다 항산화력이 높게 나타내었으나, ABTS radical 소거 능력은 열수추출물이 발효추출물 보다 항산화력이 높게 나왔다. MTT assay를 이용한 추출물의 세포독성 실험에서는 청양고추와 녹광고추 두 추출물의 모든 농도에서 세포독성이 미약한 것으로 확인되었다. 따라서 고추 수확 후 버려지는 잔재물이 천연 기능성 물질추출을 위한 소재로 이용되거나 또한 그 추출물이 각종 바이오 소재로 활용되어도 큰 문제가 없으리라 판단된다. The purpose of this study was to investigate the value of residue discarded after harvesting a species of pepper (Capsicum annuum L.). This study analyzed the physiological activity and cytotoxicity of a fermented extract and a hot water extract gathered from the residues of the Cheongyang pepper and the Nokkwang pepper. The total nitrogen, phosphoric acid, potassium, calcium, and magnesium contents of the fermented extract of the Nokkwang pepper were approximately twice as high as those of the Cheongyang pepper, while the hot water extracts had similar levels of these components across both peppers. Among the microelements, only boric acid, iron, and silicon components were detected, whereas zinc, manganese, molybdenum, and copper components were not detected in either extract. The total polyphenol and flavonoid contents of the fermented extracts were more than two times higher than those of the hot water extracts for both peppers. The DPPH radical scavenging ability (indicating antioxidant activity) of the fermented extract was higher than that of the hot water extract, while the ABTS radical scavenging ability (indicating antioxidant activity) of the hot water extract was higher than that of the fermented extract. A cytotoxicity test was conducted on the extracts using an MTT assay. Both extracts exhibited weak cytotoxicity at all concentrations for both peppers. These results suggest that the extract of residue discarded after harvesting these peppers could be suitable for functional feed or agricultural materials.

양파 수확 후 잔재물을 이용한 발효 및 열수 추출물의 생리활성과 세포독성 분석

김태원(Tae-Won Kim),이건희(Geon-Hee Lee),전병균(Byeong-Gyun Jeon),이성호(Sung-Ho Lee) 한국생명과학회 2018 생명과학회지 Vol.28 No.10

본 연구에서는 양파 수확 후 버려지는 잔재물의 활용가치를 위해 이들로부터 추출한 발효 추출물과 열수 추출물의 생리활성과 세포독성을 분석하고자 하였다. 추출물의 pH는 모두 산성을 나타내었고, 유기물 함량은 발효추출물에서 0.75%로 열수 추출물의 0.19% 보다 4배 많이 함유되었다. 다량원소 중 칼륨성분을 제외한 질소, 인산, 칼슘, 마그네슘성분의 함량은 발효 추출물에서 열수 추출물 보다 높게 나타내었고, 미량원소 중 철과 규소성분의 함량도 발효 추출물에서 열수 추출물 보다 높았으며, 반면에 붕소성분의 함량은 열수 추출물에서 발효 추출물보다 높게 검출되었다. 총 폴리페놀 함량은 발효 추출물에서 16.2±3.3 ㎎․g<SUP>-1</SUP>로 열수 추출물의 총 폴리페놀 함량인 14.6±1.4 ㎎․g<SUP>-1</SUP> 보다 1.6 ㎎․g<SUP>-1</SUP> 높게 나타내었다. 반면에 총 플라보노이드 함량은 열수 추출물에서 4.8±0.7 ㎎․g<SUP>-1</SUP>로서 발효 추출물의 함량인 0.1±0.1 ㎎․g<SUP>-1</SUP> 보다 4.7 ㎎․g<SUP>-1</SUP> 높게 나타내었다. DPPH와 ABTS radical 소거능력은 모두 열수 추출물에서 발효 추출물 보다 높은 항산화력을 보였다. MTT assay를 이용한 추출물의 세포독성 실험에서는 발효 추출물과 열수 추출물에서 각각 101.6%와 97.9%의 세포생존율을 나타내어 두 추출물 모두 세포독성이 없는 것으로 확인되었다. In order to utilize the residue that is thrown away after an onion harvest, we analyzed the physiological activity and cytotoxicity of fermented and hot water extracts of the residue. The pH of the extracts were all acidic, and organic matter content was 0.75% in the fermented extract and four times more than 0.19% in the hot water extract. The contents of nitrogen, phosphoric acid, calcium, and magnesium components, except for the potassium component among macroelements, were higher in the fermented extract than in the hot water extract. The content of iron and silicon among the microelements was also higher in the fermented extract than in the hot water extract. In addition, the content of boron was higher in the hot water extract than in the fermented extract. The total polyphenol contents of the fermented and hot water extracts were 16.2±3.3 ㎎・g<SUP>-1</SUP> and 14.6±1.4 ㎎・g<SUP>-1</SUP>, respectively, which was 1.6 mg・g<SUP>-1</SUP> higher in the fermented extract than in the hot water extract. However, the total flavonoid contents of the fermented and hot water extracts were 0.1±0.1 ㎎・g<SUP>-1</SUP> and 4.8±0.7 ㎎・g<SUP>-1</SUP>, respectively, which was 4.7 ㎎・g<SUP>-1</SUP> higher in the hot water extract than in the fermented extract. DPPH and ABTS radical scavenging ability for antioxidant activity were higher in the hot water extract than the fermented extract. The cytotoxicity of the extract using MTT assay showed cell viability of 101.6% and 97.9% in the fermented and hot water extracts, respectively. It was confirmed that there was no cytotoxicity in either extract.

녹차의 폴리페놀류인 에피갈로카테킨-3-갈레이트에 의한 항노화, 항비만 및 항암효과에 대한 비교 분석

임은지(Eun-Ji Lim),김민재(Min-Jae Kim),김현지(Hyeon-Ji Kim),이성호(Sung-Ho Lee),전병균(Byeong-Gyun Jeon) 한국생명과학회 2018 생명과학회지 Vol.28 No.10

본 연구에서는 다양한 사람의 암세포주(SNU-601, MKN74, AGS, MCF-7, U87-MG 및 A-549)와 정상세포주[MRC-5 섬유아세포, 사랑니 유래 중간엽 줄기세포(DSC), 3T3-L1 생쥐의 지방전구세포]에 녹차에 포함되어 있는 epigallocatechin-3-gallate (EGCG)를 처리하여 세포 증식, 세포 노화, 지방세포로의 분화, 말단소립복원효소 활성과 암세포의 전이 능력 등을 검증하여 항노화, 항비만 및 항암 효과를 서로 비교 조사하였다. MTT 분석에서 다양한 암세포주는 정상세포주보다 유의적으로 낮은 반억제농도값을 나타내었다. 10 uM의 EGCG가 포함된 배양액에서 정상체세포인 MRC-5와 DSC를 5 계대배양한 결과 세포증식 및 세포 노화에 큰 변화를 관찰하지 못하였고, 3T3-L1 생쥐의 지방전구세포를 지방분화 배양액에 EGCG를 첨가하여 지방세포로의 분화 억제를 유도하였지만 지방세포로의 분화를 역시 억제하지 못하였다. 그러나 여러 다양한 암세포주에 10 uM의 EGCG가 포함된 배양액에 배양한 결과 암세포의 세포증식 억제, 세포노화 유도, 말단소립복원효소 활성과 암세포의 전이 능력이 현저히 감소됨을 관찰하여 EGCG는 항노화나 항비만 효과보다는 항암효과에 더 효율적인 것으로 관찰되었고, 적당한 농도에서 잠재적인 항암물질로의 한 종류로 판단된다. The study compared the anti-aging, anti-adipogenesis, and anti-tumor effects of epigallocatechin-3-gallate (EGCG) in various cancer cell lines (SNU-601, MKN74, AGS, MCF-7, U87-MG, and A-549) and normal cell lines (MRC-5 fibroblasts, dental tissue-derived mesenchymal stem cells [DSC], and 3T3-L1 pro-adipocytes). Half inhibitory concentration (IC50) values were significantly (p<0.05) higher in normal cell lines (~50 uM), when compared to that in cancer cell lines (~10 uM). For anti-aging effects, MRC-5 and DSC were exposed to 10 uM EGCG for up to five passages that did not display any growth arrest. Population doubling time and senescence-related β-galactosidase (SA-β-gal) activity in treated cells were similar to untreated cells. For anti-adipogenic effects, mouse 3T3-L1 pre-adipocytes were induced to adipocytes in an adipogenic differentiation medium containing 10 uM EGCG, but adipogenesis in 3T3-L1 cells was not inhibited by EGCG treatment. For anti-tumor effects, the cancer cell lines were treated with 10 uM EGCG. PDT was significantly (p<0.05) increased in EGCG-treated SNU-601, AGS, MCF-7, and U87-MG cancer cell lines, except in MKN74 and A-549. The level of telomerase activity and cell migration capacity were significantly (p<0.05) reduced, while SA-β-gal activity was highly up-regulated in EGCG treated-cancer cell lines, when compared to that in untreated cancer cell lines. Our results have demonstrated that EGCG treatment induces anti-tumor effects more efficiently as noted by decreased cell proliferation, cell migration, telomerase activity, and increased SA-β-gal activity than inducing anti-aging and anti-adipogenesis. Therefore, EGCG at a specific concentration can be considered for a potential anti-tumor drug.