친환경농업 - 유채재배로 농촌경관도 보존하고 청정에너지도 생산!

이영화,Lee, Yeong-Hwa 전국농업기술자협회 2012 농업기술회보 Vol.49 No.3

유채는 유지작물로 주 용도는 기름이다. 유채기름은 튀김용과 샐러드유로 많이 쓰이며, 식용 이외에도 바이오디젤, 윤활유, 인쇄잉크, 페인트, 화장품, 플라스틱 가소제 등 공업적인 용도도 증대되고 있다. 부산물인 유채박은 가축사료와 유기질 비료로 사용되고, 유채꽃은 밀원 및 관상용으로 쓰이고 있어 그 용도는 매우 다양하다.

유채 꽃가루 분말을 이용한 효모 배양배지 제조

이영화,김광수,장영석,최인후,이경보,Lee, Yong-Hwa,Kim, Kwang-Soo,Jang, Young-Seok,Choi, In-Hu,Lee, Kyeong-Bo 한국응용과학기술학회 2015 한국응용과학기술학회지 Vol.32 No.1

본 연구는 유채 꽃가루 분말의 영양성분 분석과 파쇄한 꽃가루 분말을 이용하여 효모 배양용 액체배지와 고체배지를 제조하여 효모 배양효율을 분석하였다. 유채 꽃가루 분말(100 g)은 탄수화물 58.9 g, 단백질 20.8 g, 지방 4.1 g, 회분 2.5 g 및 수분 13.7 g으로 구성되었다. 당질 중에서는 Fructose(13.7 g), Glucose(11.1 g)와 Sucrose(6.6 g), 미네랄 중에서는 K(606.7 mg)와 P(603.3 mg) 함향이 높았다. 유리 아미노산은 Glutamic acid(2,482.4 mg), Aspartic acid(2,136.5 mg), Lysine(1,648.3 mg), 및 Leucine(1,631.1 mg) 순으로 많았다. 유채 꽃가루 분말을 단독 또는 NaCl을 첨가하여 액체배지 및 고체배지 제조하고 효모를 배양한 결과, 꽃가루 분말이 15 g/L 이상 함유된 액체배지는 효모 배양 후 건물중이 YPD 배지(대조구)에 비해 많았고, 꽃가루 분말의 농도 의존적으로 효모 건물중이 증가하였다. 또한 액체배지에 NaCl을 1~20 g/L 추가 시 배양된 효모의 건물중이 한층 더 증가하였다. 유채 꽃가루로 제조한 고체배지를 이용하여 효모를 배양(48시간)한 결과, 콜로니(colonies) YPD 배지와 유사하게 잘 자랐다. The aim of this study was to analyze nutritional compositions of rapeseed pollen granules and to determine the possible usage of pollen granules as a yeast culture medium. Rapeseed pollen granules (per 100 g) were consisted of carbohydrate 58.9 g, protein 20.8 g, fat 4.1 g, ash 2.5 g and water 13.7 g. And fructose (13.7 g), glucose (11.1 g), and sucrose (6.6 g) of sugars and K (606.7 mg) and P (603.3 mg) of minerals were highly contained. In addition, free amino acids such as glutamic acid (2,482.4 mg), aspartic acid (2,136.5 mg), lysine (1,648.3 mg), and leucine (1,631.1 mg) were present at a higher level. When liquid medium, which was made from cracked pollen granules (5, 10, 15, 20, 25, 30, and 40 g/L), was tested for yeast culture, liquid medium containing pollen granules over 15 g/L showed higher yeast growth than YPD medium (control). Liquid medium containing both cracked pollen granules (15 g/L) and NaCl (1 ~ 20 g/L) improved yeast growth than the liquid medium without NaCl. In addition, when yeast growth was tested on solid medium made from pollen granules (15 g/L) at $30^{\circ}C$ for 2 days, yeast colonies were equally well-formed like those grown on YPD medium. Overall, rapeseed pollen granules have potential properties on yeast growth and could be used as a primary source for yeast culture.

Ti/Au 이중층을 이용한 초전도 상전이 센서 제작

이영화,김용함,Lee, Young-Hwa,Kim, Yong-Hamb 한국전기전자재료학회 2008 전기전자재료학회논문지 Vol.21 No.10

We report on the development of transition edge sensors for x-ray detection. The sensor technology was based on the fabrication of a superconducting film on a thin membrane. A bilayer of a superconductor, Ti, and a noble metal, Au, was e-beam evaporated on a micromachined SiNx. Another Au layer was evaporated on the two side edges of the bilayer in order not to be affected by structural imperfections at the boundaries. With the method described in the present report, the superconducting transition temperature of the device was consistently achieved to near 80 mK with a sharp transition. The energy spectrum ueasured with the device provided 37 eV FWHM for 5.9 x-rays. We also discuss the design and fabrication considerations as well as the performance of the device in detail.

Ni-Cr계 합금을 이용한 정밀 박막저항체의 제조 및 특성

이영화,박세일,김국진,임영언,Lee Young Hwa,Park Se Il,Kim Kook Jin,Ihm Young Eon 한국전기전자재료학회 2006 전기전자재료학회논문지 Vol.19 No.1

Precision thin film resistors using evanohm R alloy were fabricated by do magnetron sputtering method. The physical and electrical properties of the resistors were studied after treatment of thermal annealing. The crystallization of the film was increased as the annealing temperature increase. Diffusion and oxidation of Cr and Al elements were occurred into the film surface. The minimum TCR values of 10.46 ppm/$^{\circ}C$ and 10.65 ppm/$^{\circ}C$ were measured at the annealing temperatures of $200^{\circ}C$ and $300^{\circ}C$, respectively. We are conducting additional studies to improve characteristics of our resistors for practical device application.

감마선 검출을 위한 초전도 상전이 센서

이영화,김용함,Lee, Young-Hwa,Kim, Yong-Hamb 한국초전도학회 2008 Progress in superconductivity Vol.9 No.2

We are developing a sensitive gamma ray spectrometer based on superconducting transition edge sensors. The detector consists of a small piece of high purity Sn as an absorber and a Ti/Au bilayer as a temperature sensor. It is designed to measure the thermal signal caused by absorption of gamma rays. The mechanical support and the thermal contact between the absorber and the thermometer were made with Stycast epoxy. The bilayer was formed by e-beam evaporation and patterned by wet etching on top of a $SiN_X$ membrane. A sharp superconducting transition of the film was measured near 100 mK. When the film was biased to the edge of the transition, signals were observed due to single photon absorption emitted from an $^{241}Am$ source. The measured spectrum showed several characteristic peaks of the source including 59.5 keV gamma line. The full with at half maximum was about 900 eV for the 59.5 keV gamma line. The background was low enough to resolve low energy lines. Considerations to improve the energy resolution of the gamma ray spectrometer are also discussed.

단클론 항체를 이용하여 정제한 톡소포자충 30 kDa 항원의 면역학적 특성

이영화,노태진,신대환,Lee, Yeong-Hwa,No, Tae-Jin,Sin, Dae-Hwan 대한기생충학회 1997 The Korean Journal of Parasitology Vol.35 No.1

The molecular weight 30 kDa membrane protein of Toxoplusma Sondii (Toxoplasma 30 kDa) apparently conserved in most strains of T. gondii and sera of infected hosts. The present study aimed to elucidate Toxoplasmc 30 kDa as a useful diagnotic antigen for serodiagnisis of toxoplasmosis by ELISA and for induction of protective immunity. Murine spleen cells immunized with the membrane antigen of T. gondii were fused with mouse Sp2/0-Ag 14 myeloma cells. Out of 8 clones selected, five were IgG2b, the others belonged to IgG 1 and IgG2a. The 30 kDa antigen was distributed mainly on the surface membrane of tachyzoites by indirect fluorescence method. Murine peritoneal macrophages which were activated by 30 kDa antigen produced more amounts of NO2 compared with crude antigen-treated group, however there were no significant differences in toxoplamacidal activity between the two groups. Higher specificity of Toxoplosma 30 kDa antigen was recognized for serodiagnosis of toxoplasmosis than the crude antigen. From these results, ToxopLasmo 30 kDa antigen enhances the cytotoxic effect of macrophages as well as a more reliable means for the serodiagnosis of toxoplasmosis by ELISA. Key words: Toxoplosma gondii, 30 kDa antigen (p30), mouse, serodiagnosis, macrophage, cytotoxicity. 톡소포자충(ToxopLasmn gondii)은 다양한 항원을 가지고 있으며, 이들 항원의 분석은 세 포매개성 면역반응 및 톡소포자충증의 면역학적 진단방법의 연구에 매우 중요하다 본 연구는 톡소포자충의 여러 단백질중 대부분의 충주(strain)에 존재하는 분자량 30 kDa의 단백질을 단클론 항체를 이용하여 분리한 후. 30 kDa 항원의 면역학적 특성을 초음파 추출 조항원과 비교 평가하였다. 톡소포자충의 세포막 항원으로 면역한 마우스 비장세포와 마우스 Sp2/0-Agl4 골수종세포를 융합하여 8개의 단클론 항체를 Western blot으로 확인하였다 이들 단클론 항체는 높은 특이성을 보였으며, $IgG_{2b}가{\;}5개,{\;}IgG^{1}이{\;}2개,{\;}IgG_{2a}$가 1개였다. 간접형광항체법으로 충체내 위치를 관찰한 결과. 30 kDa 항원은 tachyzoite의 표면 세포막에 주로 분포하였다. 단클론 항체와 CNBr-activated Sepharose 4B를 coupling하여 만든 immunoafrnity chromatography를 이용 하여 30 kDa 항원을 분리하였다. 분리한 30 kDa 항원으로 자극시킨 마우스 복강대식세포의 $NO_2^{-}$ 생산량은 초음파 추출 조항원 사용군에 비해 유의하게 증가하였으나 대식세포의 탐식능은 유의한 차이가 없었다. 또한 ELISA로 톡소포자충증을 진단시, 톡소포자충 30 kDa 항원 사용군은 조항원 사용군에 비해 민감도의 변화는 없었으나 특이성은 증가하였다 이상으로 보아 톡소포자충 30 kDa 항원은 감염 방어 면역 효과가 있었으며 진단에 이용시 특이성을 더 높일 수 있었다.

방풍통성산(防風通聖散)이 L-NAME 유발 및 SHR 흰쥐의 고혈압에 미치는 영향

이영화,김경철,이용태,Lee, Young-Hwa,Kim, Kyung-Chul,Lee, Yong-Tae 대한동의생리학회 1999 동의생리학회지 Vol.14 No.2

In order to get the effect of Bangpoongtongsungsan (BPS) water extract on the blood pressure of every 5 group of rats (in normal state of after 3 hours of unanesthetized) was measured and following results were obtained. 1. BPS intraperitoneal injection was not recognized as having the effect of decreasing blood pressure compared with Control on hypertension induced by L-NAME in young rats. 2. BPS oral administration was recognized as having the effect of decreasing blood pressure compared with Control on hypertension induced by L-NAME in young rats. 3. BPS oral administration was recognized as having the effect of decreasing blood pressure compared with Control on hypertension induced by L-NAME's continual injection and BPS continual oral administration in young rats. 4. BPS oral administration was recognized as having the effect of decreasing blood pressure compared with Control on hypertension induced by L-NAME's in adult rats. 5. BPS oral administration was not recognized as having the effect of decreasing blood pressure compared with Control on spontaneous hypertension rats. According to the above results. it is known that BPS extract oral administration decreased the blood pressure of hypertension induced by and more effective to the youth.

클린뷰티 화장품에 나타난 통합 브랜드 커뮤니케이션 연구

이영화,Lee, Young-Hwa 한국융합학회 2021 한국융합학회논문지 Vol.12 No.4

코로나 시대 마스크 착용으로 유해 성분을 배제한 화장품에 대한 관심이 커지며 '클린뷰티'가 주목받고 있다. 이에 본 연구는 2020년 국내 온·오프라인에서 유통되고 있는 클린뷰티 화장품 브랜드를 선정하여 분석하였다. 클린뷰티 브랜드 36개를 추출하고 전문가 분석을 통해 적합한 브랜드 20개를 선정하였다. 클린뷰티 화장품 브랜드 커뮤니케이션 분석을 위해 구성요소를 네이밍, 로고, 색채, 패키지, 웹사이트로 도출하여 설문조사하였다. 그 결과 네이밍은 자연, 건강 등을 연상하면 떠오르는 단어, 로고는 심플한 형태의 워드마크, 색채는 녹색이나 노란색계열, 패키지는 용기바디에 가운데 맞춤으로 구성된 심플한 형태, 웹사이트는 식물과 동물, 사람의 이미지를 선호하는 것으로 나타났다. 브랜드 커뮤니케이션 구성요소를 종합해 볼 때 자연, 깨끗한, 가벼운 이미지 등을 조화롭게 활용한 것이 클린뷰티 화장품 브랜드를 선호하는 요인으로 작용한 것을 알 수 있었다. 'Clean beauty' attracts attention with an increasing interest in cosmetics without harmful ingredients as people wear masks in the age of COVID-19. Thus, this study selected and analyzed clean beauty cosmetic brands circulated in Korea on/offline in 2020. This study extracted 36 clean beauty brands and selected 20 suitable brands through an experts' analysis. For an analysis of clean beauty cosmetic brand communication, components: naming, logo, color, package, and website were drawn to conduct a survey. Preferred were the words they come up with when they think of nature or health for naming; wordmarks in a simple form for logo; greenish or yellowish for color; the simple form aligned center on the container body for package; and the images of plants, animals, and humans for website. To sum up the components, utilizing natural, clean, and light images harmoniously, acted as a factor for preferring the clean beauty cosmetic brands.

유채 종간잡종 F1과 F2 집단의 종자크기 및 지방산 조성 변화

이영화 ( Yong Hwa Lee ),이태성 ( Tae Sung Lee ),김광수 ( Kwang Soo Kim ),서태철 ( Tae Cheol Seo ),장영석 ( Young Seok Jang ),이경보 ( Kyeong Bo Lee ) 한국국제농업개발학회 2015 韓國國際農業開發學會誌 Vol.27 No.2

Seed size is an important component of yield in rapeseed (Brassica napus L.). In this study seed size (determined by 100-seed weight) in six rapeseed crosses between B. napus (maternal (♀) variety: Tammi, Tamla, Halla, Mokpo CGMS) and B. rapa (paternal (♂) variety: Sarso, Sarson, Sarsyun) were analyzed including parents and F1 and F2 generations. Differences in the mean 100-seed weight of F1 and F2 generations were detected in six crosses (Mokpo CGMS × Sarso, Mokpo CGMS × Sarson, Tammi × Sarsyun, Tammi × Sarson, Tamla × Sarsyun, Halla × Sarsyun). Mean 100-seed weight was highest in Tammi × Sarsyun cross. Several lines in F2 generations of three cross combinations (Tammi × Sarsyun, Tamla × Sarsyun, and Halla × Sarsyun) showed about 0.7g of 100-seed weight. Fatty acid compositions in the three cross combinations were further analyzed. The large seed lines were accompanied by a simultaneous increase in erusic acid that derived from paternal parents. Based upon these results, large rapeseed seed lines with low-erusic acid would be selected in subsequent generations.

순환운동프로그램이 중년여성의 신체구성, 혈중지질, 혈관탄성 및 근력에 미치는 영향

이영화(Lee, Young-Hwa),이왕록(Lee, Wang-Lok),이영란(Lee, Young-Ran) 한국체육과학회 2014 한국체육과학회지 Vol.23 No.2

The purpose of this study was to investigate the effects of circuit training on body composition, blood lipid, vascular compliance, and isotonic strength in obese middle aged women. 20 obese (percentage of body fat ≥ 30%) middle aged women were recruited from public health center. For intervention participants, their body composition (body weight, percentage of body fat, fat free mass), blood lipid profiles(TC, TG), vascular compliancd(upper hand,lower foot), and strength were measured at before and after the intervention. Subjects were divided into two groups; circuit training group (n=23), control group (n=23). First, After 12 weeks, body weight, percentage of body fat were significantly decreased in the circuit training group compared to the control group. Second, After 12 weeks, TC, TG were significantly decreased in the circuit training group compared to the control group. Also, vascular compliance and isotonic strength were significantly effectiveness in exercise groups. It was concluded that circuit training program has positive effect on promoting body composition, lipid profile, vascular compliance and isotonic strength in obese middle aged women.