국가번영을 위한 통일과 접경지역의 발전방향

안광수 ( Ahn Kwang Soo ) 한국접경지역통일학회 2017 접경지역통일연구 Vol.1 No.1

접경지역은 한국의 안보와 군사적 목적을 위하여 희생해온 지역이다. 한국의 다른 지역들이 유례없이 빠른 경제적 성장을 구가하는 동안 접경지역은 오랫동안 소외되어 왔다. 이러한 현상이 지속되어서는 안된다. 앞으로 다가올 국가의 번영과 통일을 위하여 접경지역의 중핵지대로 고려되어야 할 것이다. 접경지역의 발전을 통하여 남북의 이질성을 해소하고 상호 교류협력을 촉진시켜 나가야 할 것이다. 또한 접경지역은 안보와 분단의 교훈을 학습하는 장이 되어야 할 것이며, 자연생태를 보존하고 정교하게 계획된 개발을 통하여 발전하도록 해야 할 것이다. The borderline area of the South Korea has been dedicated to national security and military operation. It was far from development while the other area were in rapid development. This should not be carried on. To prepar for national reunification and the future prosperity, the borderline area should be considered as a heartland in the Korean peninsula. The borderline area is an area to solve the differences while promoting exchanges and cooperation between the two Koreas. It is a place where educate the importance of security and the lessons learned through separation. The natural environment should be preserved with well designed development plan.

국방연구개발 예산 체계 진단과 제언

임승혁(Seung-Hyuk Lim),안광수(Kwang-Soo Ahn) 한국과학기술기획평가원 2023 이슈페이퍼 Vol.- No.-

마우스 비장세포에서의 Polychlorinated Biphenyls(Aroclor 1234)의 면역 독성

반상자(Sang Ja Ban),안광수(Kwang Soo Ahn),김주환(Ju Hwan Kim),임종준(Jong Jun Lim),김수연(Soo Yeun Kim),기미경(Mee Kyung Kee),이명숙(Myoung Sook Lee),조수열(Soo Yeul Cho),김영훈(Young Hoon Kim),김지윤(Ji Yoon Kim),이유경(Yoo Kyoung L 한국독성학회 2003 Toxicological Research Vol.19 No.4

Polychlorinated biphenyls (PCBs) has been widely used as plasticizer, insulator, lubricant, paint and ink. The persistence of PCBs in the environment and their bioaccumulation in living<br/> organism make a raise concerns regarding their toxic effects in immune system and subsequent effects on human health. However little has been known about effect of PCB, an endocrine disrupter,<br/> on splenocytes. In this study, for identifying the effect on the organs and immune cell of mice by the concentration and time of commercial PCB mixture (Aroclor 1254), each 3 mice were tested at the concentration of 3, 30, 300, 1,000 mg/kg respectively, and their organ's weight were measured in 4, 7, 14 days, respectively. Also according to concentration and time, PCB was evaluated for the effects on splenocyte viability and lipopolysaccaride (LPS) and concanavaline A (Con A)-induced splenocyte proliferation on mice spleen. In liver and lung, there were significantly defferent by concentration and time of PCB (p<0.0001). In respect of concentration of PCB, no significant effects on mice's liver by Aroclor 1254 concentration below than 300 mg/kg were observed except at the concentration of 1,000 mg/kg doses (p<0.0001). But there was not significant different change in mice spleen by concentration and time of PCB (p=0.2206) and the mode of weight change of spleen was different to of liver and of lung. Viabilities of splenocytes were decreased following treatment with high concentration of PCB. Also, LPS and Con A-induced cell proliferations were decreased by Aroclor 1254 at 1,000 mg/kg. These data suggest that Aroclor 1254 is the immunotoxic compound that may have an effect on mouse immune system.

HIV-1 유래 렌티바이러스 벡터의 복제가능 바이러스 검출과 역가측정 분석방법 비교

장석기(Seok Kee Chang),오일웅(Il Ung Oh),정자영(Jayoung Jeong),안광수(Kwang Soo Ahn),손여원(Yeowon Sohn) 대한약학회 2005 약학회지 Vol.49 No.3

Human Immunodeficiency Virus type 1 (HIV-1) based lentivirus vector has demonstrated great potential as gene therapy vectors mediating efficient gene delivery and long-term transgene expression in both dividing and nondividing cells. However, for clinical studies it must be confirmed that vector preparations are safe and not contaminated by replication competent lentivirus (RCL) related to the parental pathogenic virus, HIV-1. In this study, we would like to establish the method for titration and RCL detection of lentivirus vector. The titration was determined by vector expression containing the green fluorescent protein, GFP in transduced cells. The titer was 1×107 Transducing Unit/ml in the GFP expression assay and 8.9×107 molecules/ml in the real-time PCR. Also, for the detection of RCL, we have used a combination method of PCR and p24 antigen detection. First, PBS/psi and VSV-G region in the genomic DNA of transduced cells was detected by PCR assay. Second, transfer and expression of the HIV-1 gag gene was detected by p24 ELISA. In an attempt to amplify any RCL, the transduced cells were cultured for 3 weeks (amplification phase) and the supernatant of amplified transduced cell was used for the second transduction to determine whether a true RCL was present (indicator phase). Analysis of cells and supernatant at day 6 in indicator phase were negative for PBS/psi, VSV-G, and p24 antigen. These results suggest that they are not mobilized and therefore there are no RCL in amplification phase. Thus, real-time PCR is a reliable and sensitive method for titration and RCL detection of lentivirus vector.

UV에 대한 Glycolic Acid의 피부세포증식 기전연구 및 억제효과

이화정 ( Hwa Jeong Lee ),이충우 ( Chung Woo Lee ),안광수 ( Kwang Soo Ahn ),윤여표 ( Yeo Pyo Yun ),표형배 ( Hyeong Bae Pyo ),조찬휘 ( Chan Hwi Cho ),홍기영 ( Ki Young Hong ),홍진태 ( Jin Tae Hong ) 대한화장품학회 2005 대한화장품학회지 Vol.31 No.3

Glycolic acid 는 과일과 우유 사탕수수에서 비롯되는 알파-hydroxy 산의 일종의 화장품 성분으로 UV-irradiate된 피부에서는 광보호와 항 염증효과 및 산화 방지 효과가 있는 것으로 알려져 있다. 그러나 UV자극에 의한 피부세포증식에 관하여 glycolic acid의 기능은 거의 알려진바 없다. Glycolic acid는 UV에 의한 hairless mouse의 피부에서 종양 발전을 억제한다는 것을 본 연구자 등이 규명한바 있다. 따라서 이번 연구에서는 UV에 의한 피부의 종양발생억제 효과가 glycolic acid가 UV에 의한 피부의 세포성장을 억제하기 때문인지를 연구하였다 Glycolic acid 를 처치한 피부에서 UV에 의하여 유도된 세포증식과 apoptotic cell death을 감소시켰다. In vitro 연구에서도 glycolic acid 는 UVB 에 의하여 유도된 피부 유래세포인 keratinocyte의 세포성장억제와 apoptotic cell death 및 caspase-3 활동을 억제하였다. 이 결과들은 glycolic acid가 UV에 의하여 유도된 피부종양발생 억제가 UV에 의한 대한 피부세포성장과 apoptotic cell death를 억제하는 효과에 의한 것임을 시사한다. Glycolic acid, an alpha-hydroxy acid derived from fruit and milk sugars, has been commonly used as a cosmetic ingredient since it was known to have photo-protective, anti-inflammatory effects, and anti-oxidant effect in UV-irradiated skin. However, little has been know about the functional role of glycolic acid on UV-induced skin cell proliferation. It was previously found that glycolic acid inhibited UV-induced skin tumor development in hairless mouse. As a possible mechanism of glycolic acid on the UV-induced skin tumor development, the ability of glycolic acid to inhibit the UVB-induced cell growth and possible mechanisms were investigated. Glycolic acid treatment attenuated the UV-induced cell proliferation and apoptotic cell death in the skin. In vitro study, glycolic acid inhibited the UVB-induced cell growth and apoptotic death through inhibiting caspase-3 activity. These results suggest that glycolic acid may exert the Inhibitory effect on the UVB-induced skin tumor development by regulating cell growth and apoptotic cell death.

한국에서 분리된 유행성이하선염 바이러스 변이에 관한 연구

민경일(Kyung Il Min),김도근(Do Keun Kim),조수열(Soo Yeul Cho),안광수(Kwang Soo Ahn),민복순(Bok Soon Min),김병국(Myoung Guk Kim),반상자(Ssang Ja Ban),허숙진(Sook Jin Hur),박순희(Sue Nie Park),이길웅(Kil Ung Lee) 대한바이러스학회 2000 Journal of Bacteriology and Virology Vol.30 No.2

화장품 원료의 피부자극성과 세포독성의 관련성

이은희(Eun Hee Lee),이종권(Jong Kwon Lee),김용규(Yong Kyu Kim),박기숙(Ki Sook Park),안광수(Kwang Soo Ahn),정경미(Kyoung Mi Jung),정해관(Jung Hai Kwan),이선희(Sun Hee Lee),정수연(Soo Youn Chung),홍진태(Jin Tae Hong) 대한약학회 2001 약학회지 Vol.45 No.3

To compare skin irritation and cytotoxicity of anti-wrinkle agents, we examined skin irritation of six anti-wrinkle agents (ascorbic acid, glycolic acid, all trans-retinoic acid, ginseng extract, retinol, EB) in New Zeland white rabbit. Cytotoxicity of these agents was determined by MTT [tetrasolium salt 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide] at multi-time points in cultured HaCaT cell, a human immortalized keratinocyte cell. We then analyzed correlation between skin irritation and cytotoxicity by spearman's rank correlation analysis. All trans- retinoic acid shewed the highest primary irritation index (0.92) in skin irritation test. Being all the six agents not irritant, retinol showed the most cytotoxic agents. The correlation between skin irritation and cytotoxicity IC50 at different time point was 0.814,0.757,0.814 and 0.7 at 3,24, 48 and 72h, respectively. We also found that IC20 and IC80 of these agents showed similar correlation with skin irritation. These results therefore demonstrated that there is close correlation between skin irritation and cytotoxicity IC50 value by MTT in HaCaT cell at early time points by anti-wrinkle agents or IC20value. IC50 at earily time point or IC20 values may be reliable alter- native determinant of skin irritation.

메스암페타민 자발섭취가 흰쥐 뇌조직 중 세로토닌 수용체에 미치는 영향

박기숙(Ki Sook Park),홍진태(Jin Tae Hong),한진이(Jin Yi Han),김혜진(Hye Jin Kim),김용규(Yong Kyu Kim),이종권(Jong Kwon Lee),안광수(Kwang Soo Ahn),이선희(Sun Hee Lee) 한국응용약물학회 2001 Biomolecules & Therapeutics(구 응용약물학회지) Vol.9 No.2

(+)-Methamphetamine (METH) is a psychostimulant, which has been the most popular abused drug in Korea. The rewarding mechanism in METH abuse has been reported to be mediated by dopaminergic system. Recently, it has been reported that dopamine releaser (phentermine) plays a dominant role in the discriminative stimulus effects of METH, whereas 5-HT releaser (fenfluramine) can strongly modify METH selfadministration. The present study is designed to assess the behavioral changes and the changes of the serotonin receptors in the brains of rats administered repeated or self-administered METH. The repeated administration of 1.0 ㎎/㎏/day METH for 12 days increased locomotor activities, and there was no difference between i.v. and i.p. treatment. Rats had actively acquired METH self-administration for 3 weeks at 0.1 or 0.2 mg/kg/injection. Whereas, it was taken few days to acquire sucrose pellet self-administration. The binding of [³H]-8-hydroxy-DPAT (5-HT_(1A) receptors) and [³H]-5-carboxytryptamine (5-HT_(1B) receptors) to brain sections was examined. Both passive administration and self-administration of METH did not change significantly the serotonin receptors levels in hippocampus, striatum and nucleus accumbens. These results suggest that serotonin receptors may not change in the acquisition period of METH self-administration, and we are trying to investigate the serotonin receptors levels of brain in rats maintained of METH self-administration.

Phthalate의 피부자극시험 및 안점막자극시험에 관한 연구

이종권(Jong Kwon Lee),김주환(Ju Hwan Kim),이은희(Eun Hee Lee),김용규(Yong Kyu Kim),홍진태(Jin Tae Hong),박기숙(Ki Sook Park),안광수(Kwang Soo Ahn),정수연(Soo Youn Chung),이선희(Sun Hee Lee) 한국독성학회 2001 Toxicological Research Vol.17 No.2

Phthalates are widely used as plasticizers to impart softness and flexibility to normally rigid polyvinylchloride products. However, there are not much studies for dermal and ocular irritation toxicity of phthalates. So we investigated the skin or eye irritation effect of some phthalates which was not reported. The primary skin irritation of diethyl phthalate (DEP), diisodecyl phthalate (DIDP) , diisononyl phthalate (DINP), dipropyl phthalate (DPP) and dipropyl phthalate (DPrP) was studied. The ocular irritation of dibutyl phthalate(DBP), DIDP, DINP, DPP and DPrP was also studied. DEP, DIDP, DINP, DPR, and DPrP were found to be non-irritating to the skin of the test animals. DBP, DIDP, DINP and DPP were found to be non-irritating to the eye of the rabbits. DPrP caused the slight irritations to the eye in 1 or 2 days after treatment but irritation of the animals was soon recovered.

Phthalate의 피부독성과 작용기전 연구

안광수,김용규,홍진태,박기숙,안광수,김주환,이은희,김혜진,임화자,남기택,김종일,이선희 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

Phthalate중에서 우리 생활애 노출될 가능성이 있는 물질중에서 기존의 연구가 안되어 있는 물질을 대상으로 피부자극을 평가하초.자 본 실험을 실시하였다. 피부자극시험은 DEP, DIDP. DMP, DPP,DPrP를 선정하여 토끼에 24시간 폐쇄 팻취한 결과 대상으로 한 phthalate 모두에저 특이한 병변이 관찰되지 않았으며 7얼이후에도 별다른 증상이 나타나지 않는 것으로 보아 꼭부자극은 없는 것으로 판단되었다. 안점막자극시험에서는 DBP, DIDP, DINP, DPP, OPrP를 각각 0_Ima씩을 토끼의 결막낭헤 투떠하여 관찰한 결과 DPrP에서 1, 2일째에 약간의 자극이 관찰되었으나 전체적으로 보아 자극이 없는것으로 관찰되었으며 나머지 phth틸ate에서는 자극이 관찰되지 않았다. 또한 phthalate중에서 사람아 약간의 자극이 있다고 알려진 DBP를 자외선과 함께 조사시 피부독성평가를 위하여 hairless mice에 UVB 0.251/cf을 2주간 조사하여 관찰한 결과, 대조군에 비하여 별다른 차이점을 발견할 수 없었으며,UV조사군에서는 피부의 손상을 관찰할 수 있었고 UV와 DBP 병용투여군에서는 UV조사군과 큰 차이를 발견할 수 없었다. 또한 UV군의 표피에서 sunburn cell의 관찰, 출혈등을 관찰할 수 있었으며 UV와 DBP 조사군에서는 UV조사군에 비해 조직학적으로 큰 '떤화가 없었다. 세포증식 변화를 알아보기 위한 BrdU(Bromodeoxyuridint) Ll(Labelling Index)는 nBP와 UV 병용투여군에서 UV군보다 수치가 높게 나타났다. 또한 UV조사군과 UV와 DBP 병웅투여군에서는 pS3 수치가 증가되었는데, 이는 apoptosis 측진인자인 Bax의 증가와 관련되었다. 이상의 결과 DBP 노출후 UV조사시 피부에 큰 병변을 일으키지는 않으며 표피세포의 약간의 증식을 가져오는 것이 확인되었다. Phthalates are widely used as placticizers to hnpart softness and flexibility to normally rigid polyviny'S chloride products. However, there are not nuch studies for dermal toxicityof phthalates. So we investigated the skin or eye irritation effect of several phthalates. Skinirritation of phthalates such as DEP, DIDP, DMP, DPP and OPrP used in this study was not foundin rabbit compared to control. Eye irritation of phthalates such as DBP, DIDP, DINP, DPP audDPrP was was not found in rabbit compared to control, except DPrP treated group, wEch showedslight irritations in 1 llr 2 days after treaDnent and then recovered. In the second part of thisstudy, the effect of DB.p on UV-exposed of mice was investigated We treated DBP on the skin ofhairless mice for 2 weeks with UVB(0.25.T/cf) exeosure. There were no siITrfficant differences between DBP and control group in the rnorphoBogicaB aspects of the skin- UV light f☞eased the thichless of epidermis and (faused the occurrence of sunburn cells in mice skin. However, therewere no significant differencrs between UV and UV+DBP group in the extent of skin changes. Interms of BrdU(bromodeofyhridine) If(labeling Index) as a proliferation marker, there weresignificant increases of BrdU M in UV than control group aRd also b)r co-treatment of DBP, theBrdU Ll was slightfy increased, which was not signiffcantly differeut. The Bevel of pS3, one of themarker of apoptosis was increased in UV group and UV+DBP group, which was related to theincrease of Bax level in UV 1nd UV+DBP group.