Pseudomonas sp. YS-542가 생산하는 Chitinase의 정제

강신욱,최희상 慶北專門大學 2003 慶北專門大學 論文集 Vol.22 No.-

The optimal condition for the production of chitinase from Pseudomonas sp. YS-542 was follow ; 1.0% colloidal chitin, 1.0% tryptone, 0.2% MgSO_(4)7H_(2)O, 0.1% K_(2)HPO_(4), pH 8.0 at 25℃ for 48hrs cultivation. The chitinase was purified by ammonium sulfate fractionation, two times of affinity adsorption. Yield of the purified enzyme was 19.2%. The purified enzyme showed a single protein band on polyacrylamide gel electrophoresis and its molecular weight was estimated to be 47,000 by SDS-polyacrylamide gel electrophoresis.

지속성 외래 복막투석 환자의 복막염

신영태,황평주,김종학,강민규,구영선,양종오,장윤경,이강욱 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

CAPD has established itself as an effective method maintaining the patients with end stage renal disease. CAPD peritonitis is one of the most important complication of peritoneal dialysis. We analyzed the incidence, pathogens, and outcome of the peritonitis, of the 95 patients who underwent CAPD at CNUH from January 1993 to July 1998. The result were as follows : 1) A total of 72 episodes of peritonitis occurred during this periods. The incidence of peritonitis were 0.64/patient/year. 2) The first episode of peritonitis was occurred within a year in 75%, within two year in 95.8%. 3) The rate of positive and negative culture were 27.8% and 72.2% respectively. Gram positive organisms were cultured in 60%, Gram negative organisms in 40%. S. aureus were most prevalent organisms(30%). 4) The cure rate were 83.3% in Gram positive organisms and 62.5% in negative organisms. 5) Peritoneal catheter were removed in 18 cases. Among them, 7 cases are due to peritonitis. In conclusion, improved technology and increasing patients' effort to careful management of catheter will reduce the incidence of peritonitis.

體育의 潛在的 敎育課程에 관한 理論的 考察

姜信旭 師範大學 體育硏究所 1988 서울大學校 體育硏究所論集 Vol.9 No.2

The hidden curriculum, as a new field, has become remarkable because it could provide an extensive impact upon educational revolution in theoretical and practical aspects. But, the hidden curriculum, which relates to affective domain, has neglected respectively in physical education. Thus it may be necessary that we understand its contents and methods for effective change of students behavior through physical activities. From this point of view, theoretical inquiries of the hidden curriculum are absolutely needed. Also, curriculum in physical education should be comprised and carried out on the basis of this hidden curriculum for its furthur development, because the hidden curriculum plays an important role of formulating teacher's behavioral model and casual & effects of their expectancy. Teacher behavior, facilities, and procedures and control of class, have more influence on curriculum in physical education rather than in other disciplines. Therefore, on the basis of this study, detailed cases and effects of the hidden curriculum in physical education should be found out for the development of the hidden curriculum in Korea.

동종골수이식 환자에서 발생한 지역사회발생 Parainfluenza virus 3형 폐렴 1예

신종욱,조요한,강철인,김성한,이상윤,김의석,오명돈,이환종,박선양,김병국,최강원 대한감염학회 2002 감염 Vol.34 No.5

중·고등학교에서의 스포츠와 사회적 지위

강신욱 師範大學 體育硏究所 1989 서울大學校 體育硏究所論集 Vol.10 No.1

The purpose of this study was to investigate the relationship between sports and social status in Korean Secondary education and school spirit It was also intended to compare results of this study with those of previous studies in foreign literatures. The subjects of this study were (n=896) cluster sampled in Seoul. The questionnaire constructed based on colaman (1961), Eitzen (1976), and Thirer & Wright (1985) were distributed to the subjects. The data were statistically analyzed using χ²at α level 0.05. In comparison with results of previous studies of North America, the results obtained from this study were as follows: 1. The social status in secondary male students was closely related to academic work, leading activity, social background and sports in that order. 2. The social status in secondary female students was closely related to leading activity, academic work, social background and sports in that order. 3. The social status determinant of female students viewed by male students was appearance, leading activity, academic work, social background, and sports in that order. 4. The social status determinant of male students viewed by female students was leading activity, academic work, appearance, social background, and sports in that order. 5. The school spirit determinant in secondary male students was educational environments tradition, entrance rate, sports team, and the others in that order. 6. The school spirit determinant in secondary female students was educational environments sports team, entrance rate, tradition, and the others in that order. It is concluded that sports does not evaluate the social status of participants unlike North America in Korean secondary education.

成人 Henoch-Schoenlein 자반증의 임상적 고찰

신영태,이강욱 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

Twenty cases of Henoch-Schoenlein purpura were studied in the Department of Internal Medicine, Chungnam National University Hospital from November 1986 to September 1992. The results were summerized as follows 1. There were 11 males and 9 females. They were 14 to 59 years old and the median value of the age was 19 years old. 2. The chief complaints on admission were purpura in 12 cases, hematuria in 4 cases and abdominal pain in 4 cases. Seven out of 20 cases had antecedent infection history; 5 cases with URI, 1 case with pneumonia and 1 case with meningitis. 3. The organ involvement were skin in all cases, kidney in 80%. gastrointestinal tract in 65% and joint in 45% of the patients. 4. Other main symptoms or signs were fever of chills, gross hematuria, melena, flank pain and nausea and vomiting in 9, 7, 6, 6 and 5 cases, respectively. 5. Abnormal hematologic or coagulation findings were leukocytosis in 45%, thrombocytosis in 15% and prolonged aPTT in 16.7% of the patients. 6. Serum C_3 were decreased in 2 out of 18 cases and C_4 were normal in all cases. Serum IgG and IgA were elevated in 27.8% and 61.6% of the cases, respectively. 7. The ASO titers were increased in 2 out of 18 casese and CRP were positive in 40% of the patients. 8. The histopathologic finding of the skin biopsies were leukocytoclastic vasculitis in all cases. 9. Nephritis were observed in 16 cases(80%). Seven out of 16 cases had history of gross hematuria. Severe proteinuria over 3.5gm/day were observed in 3 out of 16 cases. Among them, only one case showed decreased renal function. 10. Light microscopic finding of the 13 kidney biopsies showed grade Ⅰ in 1 case, grade Ⅱ in 4 cases, grade Ⅲa in 4 cases, grade Ⅲb in 3 cases and grade Ⅳ in 1 case. Immunofluorescent microscopic findings of the 12 kidney biopsies showe IgA, fibrinogen, C_3 , IgM and IgG deposits in 83.3%, 75%, 41.7%, 8.3% and 0%, respectively. 11. The outcome of the 4 cases without nephritis was symptom free status after short-term corticosteroid therapy. The outcome of he 15 cases with nephritis were disease free in 4 cases, improved in 3 cases, no changes in 6 cases and aggravated in 2 cases(13.3%) during the follow up period. The renal pathology of the two aggravated cases were grade Ⅲb. One case of them had severe proteinuria and gross hematuria and another case was older age(48 years old) compared with other patients.

운동선수의 학업 성취 격차 요인에 관한 연구

강신욱 師範大學 體育硏究所 1990 서울大學校 體育硏究所論集 Vol.11 No.2

This attempted to investigate some factors that may influence on the athlete’s academic achievement. Total of 243 male and 133 female athletes who are enrolled in Seoul physical Education High School were recruited as subjects to fulfill the purpose of this study. The refined inventory measure were delivered to the subjects and their responses were analyzed by correlation and multiple-regression in order to examine the significant factors and those effect. Some evidences were found from this study are as following: 1. Socio-Economic Status(SES) factor showed significant correlation but Teacher-Student Interaction(t-SI) and Perceived School Climate(psc) factors showed non-significant correlation. 2. Educational Input Factor possessed greater effect on athlete’s academic achievement than Educational Process Factor did. 3. Family income was the most significant sub-variable of Socio-Economic Status factor that affected on athlete’s academic achievement. 4. Significant others’ expectation and evaluation of his/her present ability was most robust sub-variable of Perceived School Climate(PSC) that influenced on athlete’s academic achievement.

Chitinase를 생산하는 Pseudomonas sp. YS-542의 분리 및 특성

강신욱,이준우,방광웅,최희상 慶北專門大學(영주경상전문대학) 2002 慶北專門大學 論文集 Vol.21 No.-

A chitinase-producing bacterium was isolated from soil by selective enrichmentment culture medium. The bacterium was identified as Pseudomonas sp. based on the data obtained from its morphological, cultural and biochemical characteristics. The isolated strain was named Pseudomonas sp. YS-542. The medium for the production of chitinase was consisted of colloidal chitin 1.0%, tryptone 1.0%, MgS04·7H2O 0.2%, K2HP04 0.1%. The optimum cultural temperature, initial pH and time for the best production of chitinase were 25℃, 48hrs and pH 8.0, respectively.

전신성 홍반성 낭창의 임상적 고찰

신영태,김종학,장윤경,양종오,구영선,강민규,황평주,나기량,이강욱,서광선 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

The epidemiology, diagnostic criteria, clinical features, symptoms and signs laboratory findings, kidney pathology, and clinicopathologic correlation of systemic lupus erythematosus(SLE) were analyzed. The 63 patients studied were managed at the Department of Internal medicine, CNUH, from January 1983 to December 1997. Kidney biopsy was performed in 53 patients out of 63 patients with SLE. The results were as follows: 1. The ratio of male to female was 1:26. They were 12 to 71 years old and mean age was 32.1 years old. The peak age incidence was 4th decades(30%). 2. The most frequent chief complaint on admission was generalized edema. Most patients complain two or more symptoms. 3. Immunologic and renal disorders were the most frequently observed in the ARA criteria of SLE. And the positive ANA, hematologic disorder, malar rash, and arthritis, were observed in order of frequency. 4. Among the 53 patients with renal biopsy, 30 patients revealed class Ⅳ lupus nephritis(56%), class II in 12 patients(23%), class V in 8 patients(15%) and class III in 2 patients(6%). 5. The cases of lupus nephritis represented as nephrotic syndrome were high in the class IV with 68% and class V with 86%. 6. Of 30 patients who can be followed up, 5 patients resulted in death(17 % of mortality). They "were 14 to 57 years old and mean age was 28 years old. Follow up duration were from 1 month to 6 years and 3 months, and mean duration was 1 year and 1 month, but 3 cases 7. The causes of death were as follows: Two cases were due to seizure and coma resulted from CNS involvement. One was sepsis due to miliary the & pneumonia. One was dilated cardiomyopathy and heart failure due to cardiac ac involvement. And the other one was sepsis and acute renal failure resulted from cellulitis. 8. Prednisolone was used in all patients basically, and methyl-prednisolone pulse therapy, antimalarials and alkylating agents were used in some cases. In the cases of combined therapy, the activity of SLE was well controlled.

혈청 HBsAg 양성인 사구체신염의 임상상 및 신조직 소견

신영태,구영선,강민규,황평주,김종학,이강욱,서광선 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.1

Hepatitis-B-associated glomerulonephritis is one of the immunologically mediated disorders associated with chronic Hepatitis B virus(HBV) infection. The frequency of HBsAg infection has been high in East Asia and it is one of the most important etiologic factors of the secondary glomerulonephritis. We reviewed the clinical and pathologic features of 29 patients with glomerulonephritis and HBs antigenemia, who were admitted to Chungnam National University Hospital from January 1988 to Febuary 1999. The results were as follows : 1) The average age of the patients was 33.8±10.4(mean±SD) and male to female ratio was 3.8. Proteinuria was present in 27 patients(93%), hematuria in 20 patients(69%) and hypertension in 17 patients(59%). Two patients(7%) manifested with renal insufficiency and 8 patients(28%) with nephrotic syndrome. 2) Renal pathology revealed membranous nephropathy(41%), membranoproliferative glomerulonephritis(21%), mesangial proliferative glomeulonephritis(21%), minimal change nephritis (14%) and focal segmental glomerulonephritis(3%). 3) The pathologic findings of liver were chronic active hepatitis(68%), chronic persistent hepatitis(38%) and liver cirrhosis(8%). 4) During the follow-up period(average:49.4 months), 7 patients(68%) presented persistent proteinuria, 5 patients(21%) progressed to renal failure and 2 patients(8%) resolved clinically. 5) The treatment was done with prednisolone to 3 patients, and with alpha-interferon to 3 patients. One patient treated with prednisolone resolved clinically, but this resolution did not coincide with disappearance of the hepatitis B antigens.