전립선의 횡문근육종

김경미,양기화,강석진,김병기,김선무 대한병리학회 1992 Journal of Pathology and Translational Medicine Vol.26 No.4

Overexpression of CIPK 15 improved tolerance to pre-harvest sprouting in rice

Dal-A Yu,Hye-Jung Lee,Joonki Kim,Me-Sun Kim,Marjohn Nino,Sothea Ouk,Seong-Dong Kim,Ill-sup Nou,Yong-Gu Cho 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Since global climate changes drastically, pre-harvest sprouting (PHS) is expected to pose serious problems in rice production. CBL-interacting serine/threonine protein kinases (CIPKs) have been implicated to play important role in regulating various abiotic stresses such as cold, salinity and drought. In this study, to understand the function of this gene under pre-harvest sprouting in rice, a cDNA clone encoding CBL-interacting protein kinase 15 was isolated from rice flowers. We constructed a recombinant vector carrying the CIPK15 under the control of the CaMV 35S promoter and Tnos terminator and transformed into rice using Agrobacterium tumefaciens. Insertion of the gene was verified in transformants using HPT resistance test and genomic PCR. Transcriptional profiling using tissues of wild type, Gopum, revealed expression of the gene in whole plant tissues with level of expression highest in the seeds suggesting possible role in dormancy. Comparative expression analysis of the gene in transgenic and wild type through semi-quantitative RT-PCR and real-time PCR showed higher expression in transgenic rice lines. Moreover, screening in the mist chamber showed overexpression lines that were resistant to the PHS. This result suggests the involvement of CIPK15 in the regulation of pre-harvest sprouting.

Overexpression of CBL-interacting protein kinase 15 improves tolerance to pre-harvest sprouting in rice (Oryza sativa L.)

Dal-A Yu,Hye-Jung Lee,Joonki Kim,Me-Sun Kim,Marjohn Nino,Sanguk Byeon,Jeongmi Kim,Illsup Nou,Kwon-Kyoo Kang,Yong-Gu Cho 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Since global climate changes drastically, pre-harvest sprouting (PHS) is expected to pose serious problems in rice production. CBL-interacting serine/threonine protein kinases (CIPKs) have been implicated to play important role in regulating various abiotic stresses such as cold, salinity and drought. In this study, to understand the function of this gene under pre-harvest sprouting in rice, a cDNA clone encoding CBL-interacting protein kinase 15 (CIPK15) was isolated from rice flowers. This gene is 2,818 bp long with 1,332 bp coding region that encodes a polypeptide of 443 amino acids. We constructed a recombinant vector carrying the OsCIPK15 under the control of the CaMV 35S promoter and Tnos terminator and transformed into rice using Agrobacterium tumefaciens. Insertion of the gene was verified in transformants using HPT resistance test and genomic PCR. Transcriptional profiling using tissues of wild type, Gopum, revealed expression of the gene in whole plant tissues with level of expression highest in the seeds suggesting possible role in dormancy. Comparative expression analysis of the gene in transgenic and wild type through semi-quantitative RT-PCR and real-time PCR showed higher expression in transgenic rice lines. Moreover, screening in the mist chamber showed overexpression lines that were resistant to the PHS. This result suggests the involvement of OsCIPK15 in the regulation of pre-harvest sprouting.

Breeding Hybrid Rice with Genes Resistant to Diseases and Insects Using Marker-Assisted Selection and Evaluation of Biological Assay

( Me-sun Kim ),( Sothea Ouk ),( Kuk-hyun Jung ),( Yoohan Song ),( Le Van Trang ),( Ju-young Yang ),( Yong-gu Cho ) 한국육종학회 2019 Plant Breeding and Biotechnology Vol.7 No.3

Developing elite hybrid rice varieties is one important objective of rice breeding programs. Several genes related to male sterilities, restores, and pollinators have been identified through map-based gene cloning within natural variations of rice. These identified genes are good targets for introducing genetic traits in molecular breeding. This study was conducted to breed elite hybrid lines with major genes related to hybrid traits and disease/insect resistance in 240 genetic resources and F1 hybrid combinations of rice. Molecular markers were reset for three major hybrid genes (S5, Rf3, Rf4) and thirteen disease/insect resistant genes (rice bacterial blight resistance genes Xa3, Xa4, xa5, Xa7, xa13, Xa21; blast resistance genes Pita, Pib, Pi5, Pii; brown planthopper resistant genes Bph18(t) and tungro virus resistance gene tsv1). Genotypes were then analyzed using molecular marker-assisted selection (MAS). Biological assay was then performed at the Red River Delta region in Vietnam using eleven F1 hybrid combinations and two control vatieties. Results showed that nine F1 hybrid combinations were highly resistant to rice bacterial blight and blast. Finally, eight F1 hybrid rice varieties with resistance to disease/insect were selected from eleven F1 hybrid combinations. Their characteristics such as agricultural traits and yields were then investigated. These F1 hybrid rice varieties developed with major genes related to hybrid traits and disease/insect resistant genes could be useful for hybrid breeding programs to achieve high yield with biotic and abiotic resistance.

Effect of Varieties and Silage Quality of Winter Crop on the Palatability of Calves

Meing-Jooung Kim,Jong Guen Kim,Sung Seo,Eui Soo Jeong,Sei Hyung Yoon,Joung Kyong Lee,Seok Ki Im,Sun Sik Jang,Oh Sub Kwon 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

This experiment was conducted to determine the effect of varieties and quality of winter crop on palatability of calves for two years. Four Italian ryegrass variety(Kospeed, Kogreen, Kowinearly and Hwasan 101ho), two barley(Youngyang, Yuyeon), and a rye (Winter green) were cultivated at experimental field of National Institute of Animal Science, Cheonan. Four early varieties(Kospeed, Kogreen, Kowinearly and Winter green)were harvested at 20th Dry matter yield of "Hwasan 101 ho" was l8,629㎏/㏊(12,141 + 6,488) and Yeongyang, Youyeon and Winter green were 14,182, 12,487 and 10,935㎏/ha, respectively. Moisture content of Hwasan 101 ho was 81.5% in harvesting time, but wilting for 24 hours might bedecrease moisture content by 65.5%. The relative intake of Hwasan 101 ho, Kowinearly, Kospeed and Kogreen were 34.5, 60, 19.8, 15.7%, respectively. Among forage crop species, relative intake of Italian ryegrass(Hwasan 101 ho), barley(Yeongyang and Youyeon), and Rye(Winter green) were 43.1, 20.9, 20.5 and 15.5%, respectively.

Classification of Korean rice varieties based on growth characteristics

Me-Sun Kim,Hye-Jung Lee,Dal-A Yu,Joonki Kim,Franz Nogoy,Eun-Ju Jeong,Jang-Hwan You,Yong-Gu Cho 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

The International Union for the Protection of New Varieties of Plants (UPOV) promotes an effective system of plant variety protection and encourages the development of new varieties of plants. International convention was initiated to standardized the system efforts and strengthen the policy. This study was conducted to establish a database for rice identification using morphological characters which include number of tillers and panicle per plant, spikelets per panicle, yield, plant maturity, height, leaf pigments, flag leaf angles, and rice bran. The whole rice population was grouped into three based on leaf angles, majority members of which retained the flag leaf angle-character until maturity stage. Most rice accessions did not exhibit anthocyanin pigments on the leaves particularly on the first leaf, leaf blade, leaf sheath and auricle, except for varieties classified as black rice. In the case of grain, many accessions produced secondary branching, and showed no awn. For agronomic traits, productive tiller and panicle per plant were higher in early flowering varieties, while spikelets per panicle and ripened grain were higher in late flowering varieties, and yield was higher in medium flowering varieties. All data were then pooled for cluster analysis which revealed three major independent clusters and four minor clusters.

Neighborhood Layered Maintenance Software Architecture of ATM Switching System

Sun Me Kim,Chan Heung Yoo,Byung Ho Yae 대한전자공학회 1996 ITC-CSCC :International Technical Conference on Ci Vol.- No.-

Molecular analysis of transgenic rice overexpressing UDP-glycosyltransferase from Brassica rapa

Me-Sun Kim,Hye-Jung Lee,Joonki Kim,Dal-A Yu,Sanguk Byeon,Kwon-Kyoo Kang,Illsup Nou,Yong-Gu Cho 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Secondary plant metabolites undergo several modification reactions, including glycosylation and physiological functions. Glycosylation, which is mediated by UDP-glycosyltransferase (UGT), plays a role in the storage of secondary metabolites and in defending plants against stress. In this study, a UDP-glucosyltransferase cDNA was isolated from Brassica rapa hereinafter referred to as BrUGT. It has a full-length cDNA of 1,236 bp that contains a single open reading frame of 834 bp which encodes a polypeptide of 277 amino acid residues with a calculated mass of 31.19 kDa. BLASTX analysis hits a catalytic domain of glycos_transf_1 super family (c112012) that belongs to the glycosyltransferases group 1 with tetratricopeptide (TPR) regions. UGT gene expression analysis showed high mRNA transcripts in pistil, followed by petal, seed and calyx of flower in Brassica rapa. Furthermore, we constructed a recombinant pFLCIII vector carrying the BrUGT gene under the control of ubiquitin promoter and NOS terminator and transformed into rice using Agrobacterium tumefaciens. The UGT overexpressing rice lines were then characterized at the physiological and molecular levels. To further understand the biological function of BrUGT, transcriptional profiling of the gene in transgenic rice lines under cold, salt, PEG, H2O2, ABA and drought stress condition is underway.

내한성 이탈리안 라이그라스 품종별 파종량이 종자생산에 미치는 영향

김맹중(Meing Jooung Kim),서성(Sung Seo),김종근(Jong Geun Kim),최기준(Ki Jun Choi),김기용(Ki-Yong Kim),이상훈(Sang-Hoon Lee),장선식(Sun Sik Chang),김태일(Tae Il Kim),권응기(Eung Gi Kwon),전병수(Byoung Soo Jeon),최기춘(Ki Choon Choi) 한국초지조사료학회 2010 한국초지조사료학회지 Vol.30 No.3

본 시험은 국내육성 내한성 이탈리안 라이그라스의 확대 보급을 위한 종자생산 시험으로 국내육성 품종인 화산 101호 및 코그린의 파종 량별 종자수량 구성요소별 특성을 구명하기 위하여 2004년 9월부터 2007년 6월까지 3년간 국립축산과학원 초지사료과 시험 포장에서 수행 되었다. 파종 재식거리는 20 cm 세조파로 파종 하였고, 시험구 면적은 12 ㎡ (3 × 4 m), 파종량은 5, 10, 20, 30, 40 ㎏/㏊ 품종별 5처리 난괴법 3반복으로 수행하였다. 채종시기는 조생종 “코그린”은 6월 16일, 중만생종 화산 101호는 7월 3일에 수확하여 조생종과 중만생종간의 채종일은 조생종이 15일 정도 빨랐다. 이탈리안 라이그라스 화산 101호의 종자수량 구성요소에서 평균 이삭수는 471개/㎡, 이삭길이 28.5 ㎝, 등숙률 71% 이었으며, 코그린의 평균 이삭수는 633개/㎡, 이삭길이 24.0 ㎝, 등숙률 82%이었다. 화산 101호의 평균 종자 생산량은 1,631 ㎏/㏊이며, 20 ㎏/㏊ 파종구에서 유의성이 인정 되었고 (p<0.05), 40 ㎏/㏊ 파종구에서 1,971 ㎏/㏊으로 가장 많은 종자생산량을 보였다. 코그린은 20 ㎏/㏊ 파종구에서 3,079 ㎏/㏊으로 종자생산량이 가장 많았으며 파종량별 유의성이 인정되었다 (p<0.05). 종자의 탈립은 품종 고유의 특성으로 파종량별 차이는 없었으나 화산 101호가 40.3%로 코그린 2.6%에 비해 매우 높게 나타났다. 따라서 화산 101호의 채종량이 코그린에 비하여 감소되는 원인은 탈립에 의한 것으로 사료된다. 또한 채종된 종자의 화산 101호는 채종 직후부터 발아하여 81.8%의 발아율을 보였으나 코그린은 채종 직후에는 발아율이 낮아 저온 저장 후 발아율이 79.4%로 회복되었다. 이상의 결과를 종합하여볼 때 내한성 이탈리안 라이그라스 화산 101호는 탈립에 의해 파종량을 30~40 ㎏/㏊으로 증가시켜야 하고 코그린의 파종량은 20 ㎏/㏊이 적정량으로 권장되었다. This experiment was carried out to investigate the effect of seeding rate of newly developed Italian ryegrass varieties, “Kogreen” and “Hwasan 101”, on those seed production in Cheonan for three years (2005 to 2007). Two Italian ryegrass varieties were seeded at experimental fields of Grassland and Forage Division, National Institute of Animal Science on late September, and harvested at seed shattering stage. The experiment was arranged in randomized complete block design with three replications by each variety. The treatments was consisted of five seeding rates (5, 10, 20, 30, 40 ㎏/㏊). The average heading date of “Kogreen” was 9 May and “Hwasan 101” was 25 May. The harvesting date of “Kogreen” was on 16 June when the rainy season should not set in. In the case of “Hwasan 101”, the harvesting date of seed production was 3 July, because of rainy season. The number of ear per square meter, ear length, spikelet number, seed yield per ha and ripening rate of “Hwasan 101” were 471, 28.5 ㎝, 24.8, 1,631 ㎏ and 71%, respectively. and those of “Kogreen” were 633, 24.0 ㎝, 20.8, 2,676 ㎏ and 82.1%, respectively. The shattering property rate of “Hwasan 101” 40.3% was higher than that of “Kogreen” 2.6%. The germination rate of harvested “Hwasan 101” seed was 81.8% and that of “Kogreen” was 79.4%, respectively. In conclusion, the optimum seeding rate of Italian ryegrass for seed production are recommended 20㎏/㏊ in both “Hwasan 101” and “Kogreen”.

논에서 이탈리안 라이그라스 품종별 파종시기가 월동 및 건물수량에 미치는 영향

김맹중(Meing Jooung Kim),최기준(Ki Jun Choi),김종근(Jong Geun Kim),서성(Sung Seo),윤세형(Sei Hyung Yoon),임영철(Young Chul Lim),임석기(Seok Ki Im),권응기(Eung Gi Kwon),장선식(Sun Sik Chang),김형철(Hyeong Cheol Kim),김태일(Tae Il Kim 韓國草地學會 2009 한국초지조사료학회지 Vol.29 No.4

논에서 내한성 이탈리안 라이그라스의 조중 만생 숙기별 Kospeed, Kowinmaster, Hwasan 101호 3품종을 파종시기별로 9월 30일부터 5일간격으로 10월 20일까지 5처리 파종하여 이듬해 5월 17일에 수확하였고 단위면적당 입모수, 월동률 및 건물수량에 미치는 시험을 2003년부터 2005년까지 수원에서 수행하였다. 논에서 이탈리안 라이그라스 조생종(Kospeed)의 출수기는 5월 7일에서 5월 13일이며 파종일이 늦으면 출수일도 늦어졌다. 또한 조생품종 이탈리안 라이그라스는 개화최성기에 수확할 수 있어 건물수량이 높고 모내기를 적기에 할 수 있었다. 중생종(Kowinmaster)은 5월 16일에 출수하였고, 만생종(Hwasan 101)은 생육시기가 늦어 출수 전에 수확하여 모내기를 5월 20일에 마쳤다. 중부지방에서 적기 모내기를 위해 5월 17일 수확한 건물수량은 9월 30일에 파종한 시험구의 조생종 Kospeed는 7,909 ㎏/㏊이며, 중생종 Kowinmaster 6,398 ㎏/㏊, 만생종 Hwasan 101호 5,204 ㎏/㏊이었다. 건물수량이 높은 조생종(Kospeed)의 논에서 적정 파종시기는 9월 30월에서 10월 5일까지이며 중 만생종은 파종시기가 빨라도 조생종보다 건물수량이 감소하였다. 파종시기에서 9월 하순에서 10월 5일까지는 건물수량이 차이가 없었으나 그 이후는 월동률도 급격히 떨어져지고 건물수량도 감소하였다. 또한 중 만생종은 숙기가 늦어 수분함량이 많아 사일리지 품질에도 영향을 미칠 것으로 판단된다. 따라서 중부지방에서 내한성 이탈리안 라이그라스의 논 재배는 조생종 품종으로 10월 5일 이전에 파종해야 건물수량을 높일 수 있을 것으로 판단한다. This experiment was carried out to investigate the effect of cultivar and seeding date on the winter survival rate, quality and DM yield of Italian ryegrass on paddy field for three years in Suwon. Seeding started from 30th Sep. 2003. at intervals of five days and finished 20th Oct. New varieties of Italian ryegrass used in this experiment were “Kospeed”, “Kowinmaster” and “Hwasan 101”. The winter survival average rate of 5th Oct. seeding plot was 89.8% and it decreased with delayed seeding date. The heading date of “Kospeed” was 7th~13rd May, “Kowinmaster” was 16th May, but “Hwasan 101 ho” didn’t show heading until 17th May. Dry matter (DM) yields of 30th Sep. seeding plot were Kospeed 7,909 ㎏/㏊, Kowinmaster 6,398 ㎏/㏊ and Hwasan 101 ho 5,204 ㎏/㏊. DM yield was decreased with delayed seeding date. Total digestible nutrient (TDN) yield was also decreased with delayed seeding date. Crude protein (CP) content was the highest in Kospeed. seeding plot as 18.3% and in vitro dry matter digestibility (IVDMD) was not showed significant difference among seeding dates.