신이식 환자에서 EBV감염과 관련된 악성 임파종 2예

윤선애,진동찬,김용수,신완식,윤영석,방병기,최영진,심상인,고용복 大韓移植學會 1993 Korean Journal of Transplantation Vol.7 No.1

대장암에서의 nm23과 CD44 단백질 발현에 대한 면역조직학적 분석

신동일,이기형,윤충,이주희 대한대장항문학회 1997 Annals of Coloproctolgy Vol.13 No.1

두개외 경동맥 협착증에 대한 스텐트 삽입술의 추적 결과

윤영섭,홍범기,최동훈,김선호,김동익,김승민,장양수,심원흠 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.11

치아회분과 석고혼합제재의 물성에 관한 연구

계기성,강동완,김광수,고영무,김윤주 대한치과기재학회 1998 대한치과재료학회지 Vol.25 No.1

The purpose of this study is to evaluate the use value of tooth ash-plaster mixture as an alternative material of the synthetic hydroxyapatite. For this purpose the author performed the experimental study to investigate the physical properties of sintered tooth ash-plaster mixture. The tooth ash was made by increasing procedure at 850℃, 900℃ and 950℃ for 20min, 40min and 60min respectively. The composition of tooth ash was analyzed using ICPSE and X-ray diffraction was done. The experimental specimens were molded to the cylindrical from 10mm high, 5mm diameter under the pressure of 1,000kg/㎠ for test of compressive strength values and 3mm high, 6mm diameter for test of diametral tensile strength values and surface microhardness values. Mixtures of tooth ash-plaster mixture was vacuum fired at 1,000℃ for 60min. The physical properties of the sintered specimen were examined and their microstructures were observed under the scanning electron microscope. The results were as follows: 1. x-ray diffraction showed the crystallization of tooth ash was developed as the incinerating temperature and time were increased, but the phase transformation following the incinerating temperature and time was not occurred. 2. The values of compressive strength of the sintered tooth ash-plaster mixture were the highest in case of incinerating temperature 950℃, the incinerating time 60min, and the tooth ash-plaster mixing weight ratio of 4:1. The higher tge incineration temperature and the longer the incineration time was, the more increased the compressive strengths were(p<0.01). 3. The values of diametral tensile strength of the sintered tooth ash-plaster mixture were the highest in case of the incinerating temperature 950℃, the incinerating time 60min, and the tooth ash-plaster mixing weight ratio of 4:1. The higher incineration temperature and the longer the incineration time was, the more increased the diametral tensile strengths were (p<0.01). The more decreased tooth ash-plaster mixing ratio was, the more increased the diametral tensile strength was, but there was not a significant difference statistically (p<0.01). 4. The values of surface microhardness of the sintered tooth ash-plaster mixture were also the highest in case of the incinerating temperature 950℃, the incinerating time 60min, and the tooth ash-plaster mixing weight ratio of 4:1. The higher the incineration temperature was, the more increased the surface microhardness values were, but there was not a significant difference statistically(p<0.01). The longer the incineration time was and the more decreased the tooth ash-plaster mixing ratio was, the more increased the surface microhardness values were. 5. The porosity and absorption of the sintered tooth ash-plaster mixture were the highest in case of the incinerating temperature 850℃, the incinerating time 20min, and the tooth ash-plaster mixing weight ratio of 3:1. The lower the incineration temperature was and the shorter the incineration time was, the more increased the porosity was. 6. There was a good wettability in the sintered tooth ash-plaster mixture under the scanning electron microscope.

Cefixime 경구제제의 생물학적 동등성 연구

김형기,권준택,손동렬,염윤기 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

The bioequivalence of generic cefixime(Pofixim^(®), Kwangmyung Pharmaceutical Co.) capsule to the cefixime capsule(Suprax^(®), Dong-A Pharmaceutical Co.) as a reference drug. Single dose of 100 mg cefixime was administered to 20 healthy male volunteers in a balanced, randomized crossover design with a washout between the two study periods. Blood samples were collected up to 14 hours and plasma concentration of cefixime was measured by well validated reverse phase high-performance liquid chromatography. Pharmacokinetic parameters were analyzed by non-compartmental analysis and ANOVA test was used for the statistical analysis of parameters. No statistically significant formulation, period, or sequence effect was encountered. Ninety percent confidence intervals of log transformed C_(max) and AUC_(t) were comprised in the stipulated 0.80-1.25 range. These results suggest that test formulation of cefixime can be declared bioequivalent with the reference, both formulations in 100 mg capsule.

Simvastatin 정제의 생물학적 동등성 연구

김형기,권준택,손동렬,염윤기 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

The bioequivalence of generic simvastatin tablet(Dongsung Pharmaceutical Co.) to the simvastatin tablet(Zocor^(®), MSD Korea Co.) as a reference drug. Single dose of 20 mg simvastatin was administered to 20 healthy male volunteers in a balanced, randomized crossover design with a washout between the two study periods. Blood samples were collected up to 12 hours and plasma concentration of simvastatin was measured by well validated LC-MS/MS. Pharmacokinetic parameters were analyzed by non-compartmental analysis and ANOVA test was used for the statistical analysis of parameters. No statistically significant formulation, period, or sequence effect was encountered. Ninety percent confidence intervals of log transformed C_(max) and AUC_(t) were comprised in the stipulated 0.80-1.25 range. These results suggest that test formulation of simvastatin is bioequivalent with the reference, both formulations in 20 mg tablet.

Micromanganese-Nodule의 選鑛에 관한 硏究(제1보)

엄제현,박동기,김익수,성일용,김종윤,김진석 三陟大學校 産業科學技術硏究所 2002 産業科學技術硏究論文集 Vol.7 No.4

For the purpose of researching of the manganese recovery from the micromanganese-nodule, the test was performed by means of the ζ-potential measuring and of the floatation experiment for manganese recovery of the micromanganese nodule obtained from siliceous deep sea clay. The floatation condition in the floatation experiment were as follow. Sample size : 65mesh∼200mesh. Condition time : 10 min. Flotation time : 4 min. Air amount : 20㎖/min. Promoter : DAS, NaDS. Kerosene Dpresser : Na_2SiO_3(Water glass) PH regulator : HCl, CaO From the above test the flotation results were summerized as follow. (1) ζ-potential of micromanganese-nodule was minus(-) in the pH 3∼12. (2) The manganese recovery from the micromanganese- nodule was the best at pH 7. (3) The manganese recovery from the micromanganese- nodule was the best using DACI 50㎎/ℓ + kerosene 200㎎/ℓ in the promoter at pH 7. (4) The best manganese recoverv of the micromanganese-nodule was 80% using DACI 50㎎/ℓ + kerosene 200㎎/ℓ in the promoter and water glass 500㎎/ℓ in the depresser at pH 7. In the futurity, continuous study of the micromanganese-nodule in many kinds of site should be required.

한국인에서의 CYP2C19 유전자형의 다형성

권준택,김형기,손동렬,염윤기 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

The S-mephenytoin 4'-hydroxylase (CYP2C19) metabolizes a number of clinically used drugs and shows a marked interethnic difference in the incidence of the poor metabolizer (PM). In the present study, we genotyped 215 healthy unrelated Koreans (64 females, aged 20-41 yr, residing in Chungcheong province) for functionally defective alleles, CYP2C19_(m1) and CYP2C19_(m2). Detection of the normal (CYP2C19_(wt)) and defective alleles was performed by polymerase chain reaction/restriction enzyme analysis. The genomic DNA was isolated from peripheral blood. The allelic frequencies of the wild-type (CYP2C19_(wt)) and CYP2C19_(m1) were 72.6% and 27.4%, and the wild-type (CYP2C19_(wt)) and CYP2C19_(m2) were 85.1% and 14.9%, respectively. For each CYP2C19_(m1) and CYP2C19_(m2), the observed numbers of three genotypes were similar to those calculated in accordance with the Hardy-Weinberg equation. The frequencies of homozygotes for CYP2C19_(m1), CYP2C19_(m2) and compound heterozygotes were 9.7%, 2.8% and 4.2%, respectively. The mutants of CYP2C19 were identified in 36 subjects (16.7%). These results suggest that frequency of mutants of CYP2C19 in Koreans resembled the Orientals rather than Caucasians.

한국인에서의 CYP2E1 유전자형

권준택,김형기,손동렬,염윤기 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Cytochrome P4502E1 (CYP2E1) is inducible by ethyl alcohol and activates procarcinogenic N-nitrosodimethylamine (NDMA), benzene, urethane and other lower molecular weight compound. CYP2E1 is also involved in metabolism of certain drugs, for example, alcohol, acetaminophen, tamoxifen, theophylline, fluorinated anesthetics, and chlorozoxazone. CYP2E1 activity was shown to be polymorphically distributed in humans and has been suggested to play a role in hepatocellular carcinoma and alcoholic related disorders. Although genetic predisposition to alcoholism and alcoholic liver disease has been reported, genetic susceptibility to alcoholic pancreatitis is still a matter of debate. The aims of this study were to investigate the allelic frequency of CYP2E1 Rsa I polymorphism in Koreans. We investigated the frequency distribution of CYP2E1 Rsa I polymorphism in 212 unrelated healthy Koreans and 42 hepatocellular carcinoma patients. Detection of the CYP2E1 alleles was performed by polymerase chain reaction and restriction fragment length analysis. The genomic DNA was isolated from peripheral blood with conventional phenol: chloroform extraction method. The allelic frequencies of c1 and c2 in healthy volunteers were 0.85 and 0.15, respectively. The rare type (c2/c2) of CYP2E1 Rsa I polymorphism was 0.005 in healthy volunteers.

황산화 부선법에 의한 방연광의 억제에 관한 연구

엄제현,박동기,김익수,성일용,김종윤,김진석 三陟大學校 産業科學技術硏究所 2003 産業科學技術硏究論文集 Vol.8 No.1

The applicability of a sulphation-floatation process was considered in order to depress of galena in a mineral processing process of complex sulphide ores. The galena will be depressed in the floatation step because of the formation of a PbSO₄ film on the surface of galena. when a PbS bulk concentrate is treated with sulphuric acid. The optimum conditions of the sulphation treatment for depressing galena were found to be 30%. and about 5 minutes of retention, with 18 Vol. % H₂SO₄. under these conditions. galena was sulphated only slightly and thus in complete depression in the floatation.