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지단백과 알부민 및 이들의 변형물질이 메산지움세포 증식에 미치는 영향
손일석,이태원,박재경,김희진,조병수,임천규,김명재 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2
목 적 : 혈청 지단백과 알부민은 그 자체로써 또는 산화나 당화 과정에 의한 변형을 거쳐 사구체 질환을 진행시킬 수 있는 것으로 알려져 있다. 그 기전은 분명하지는 않으나 메산지움세포 증식과 관련이 있는 것으로 알려져 있다. 저자들은 비교적 간단한 흡광도측정법을 이용하여 지단백과 알부민 및 그들의 변형물질들이 각각 메산지움세포 증식에 미치는 영향을 알아보고자 하였다. 방 법: 정상인의 혈청 지단백을 원심분리 하여 저밀도지단백, 고밀도지단백, 초저밀도지단백을얻고, 산화 저밀도지단백, 당화 저밀도지단백, 그리고 혈청 알부민과 당화 알부민을 각각 농도를 달리하여 배양 중인 인간 메산지움세포에 가한 후 흡광도를 측정함으로써 메산지움세포의 증식정도를 알아보았다. 양성 대조군으로 interleukin-1β를 이용하였다. 결 과: 저밀도지단백은 농도 증가시 50 μg/mL와 100 μg/mL에서 세포의 증식을 보였으며,고밀도지단백과 초저밀도지단백은 농도에 따른 유의한 변화가 없었다. 산화 저밀도지단백은5 μg/mL에서부터 세포의 증식을 초래한 후 10 μg/mL와 25 μg/mL에서도 이를 유지하였다. 당화 저밀도지단백은 농도 증가에 따라 10 μg/mL와 100 μg/mL에서 세포의 증식을 억제하였다. 알부민은 농도 500 μg/mL에서 세포 증식을 억제하다가 고농도(1,000 μg/mL)에서는 영향이 없었으며, 당화 알부민 역시 100 μg/mL의 적은 농도에서는 억제효과를 보였으나 고농도(1,000 μg/mL)에서는 오히려 증식을 일으키는 결과를 보였다. 결 론: 저농도에서 저밀도지단백과 산화 저밀도지단백은 사람 메산지움세포의 증식을 초래하였으며, 당화 저밀도지단백은 증식을 억제하였다. 알부민은 일정농도까지 사람 메산지움세포의 증식을 억제하다가 고농도에서는 영향이 없었으며, 당화 알부민도 일정농도까지 증식 억제 효과가 있다가 고농도에서는 오히려 증식을 초래하는 양상을 보였다. Background : Modified lipoproteins may be involved in nephro- and glomerulosclerosis. Diabetic nephropathy-like lesions have also been induced in a rat model by glycated and glycoxidized albumin. In cultured rat or human mesangial cells, enhanced cell proliferation and production of mesangial matrix in response to lipoproteins and their modified forms have been demonstrated by [3H]-thymidine incorporation and cell counting assays. But these methods are relatively complex and most of them have used only one or two of the lipoprotein, albumin and their modified forms. Methods : We investigated the effects of native and modifed lipoproteins, and albumin on cultured human mesangial cell proliferation using non-radioactive colorimetric method by MTS/PMS assay. Lipoproteins added were low density lipoprotein(LDL), high density lipoprotein(HDL), very low density lipoprotein(VLDL), oxidized LDL(oxidation with copper sulfate in vitro) and glycated LDL and we also used albumin, glycated albumin, and interleukin-1β as a positive control. Results : Interleukin-1β promoted the proliferation of cultured human mesangial cells up to concentration 20 ng/mL. LDL induced the proliferation of mesangial cells in a concentration-dependent manner up to concentration 100 μg/mL. HDL and VLDL had no significant proliferative effect. Oxidized LDL caused the proliferation of mesangial cells at low concentration up to concentration 25 μg/mL. Addition of glycated LDL resulted in a concentrationdependent inhibition of mesangial cells. Albumin and glycated albumin inhibited the proliferation of mesangial cells at low concentration of 100 μg/mL, but cell growth was increased at higher concentrations. Conclusion : We demonstrated the effects of the single and modified proteins on the proliferation of cultured human mesangial cell by relatively simple colorimetric method. Results were almostly identical to those of previous studies obtained by radioactive method or cell counting assay. (Korean J Nephrol 2002;21(2):266-275)
Il Suk Sohn,Jong Chun Park,Jae Hun Chung,Kye Hun Kim,Young Keun Ahn,Myung Ho Jeong,Jeong Gwan Cho 대한내과학회 2006 The Korean Journal of Internal Medicine Vol.21 No.2
Eosinophilic myocarditis usually results from myocardial damage as a result of drugs or parasites, and is generally associated with increased peripheral eosinophil count. This form of myocarditis is difficult to diagnose clinically. A 25 year-old previousl
( Hyun Il Seo ),( Dae Sung Lee ),( Eun Mi Yoon ),( Min Jung Kwon ),( Hyosoon Park ),( Yoon Suk Jung ),( Jung Ho Park ),( Chong Il Sohn ),( Dong Il Park ) 대한장연구학회 2016 Intestinal Research Vol.14 No.2
Background/Aims: To prevent the transmission of pathogens by endoscopes, following established reprocessing guidelines is critical. An ideal reprocessing step is simple, fast, and inexpensive. Here, we evaluated and compared the efficacy and safety of two disinfectants, a tertiary amine compound (TAC) and ortho-phthalaldehyde (OPA). Methods: A total of 100 colonoscopes were randomly reprocessed using two same automated endoscope reprocessors, according to disinfectant. The exposure time was 10 minutes for 0.55% OPA (Cidex® OPA, Johnson & Johnson) and 5 minutes for 4% TAC (Sencron2®, Bab Gencel Pharma & Chemical Ind. Co.). Three culture samples were obtained from each colonoscope after reprocessing. Results: A total of nine samples were positive among the 300 culture samples. The positive culture rate was not statistically different between the two groups (4% for OPA and 2% for TAC, P =0.501). There were no incidents related to safety during the study period. Conclusions: TAC was non-inferior in terms of reprocessing efficacy to OPA and was safe to use. Therefore, TAC seems to be a good alternative disinfectant with a relatively short exposure time and is also less expensive than OPA. (Intest Res 2016;14:178-182)
뇌졸중에 관한 역학적 연구 - 제 1 보 뇌졸중의 진단기준과 임상적 관찰
손의석 ( E Suk Sohn ),강성효 ( Sueng Hyo Kang ),배종화 ( Jong Wha Bae ),윤재덕 ( Jai Duck Yoon ),박승철 ( Seung Chul Park ),이종석 ( Chong Suk Lee ),안승운 ( Seung Oon Ahn ),최일영 ( Il Young Choi ) 대한내과학회 1968 대한내과학회지 Vol.11 No.3
In spite of the fact the cenebrovaacular accident is the most dominant cause of death in Korea. There are only a few papers so far published in regards to this very important disease. The purpose of this study is to investigate the carebrovascular accident
한국인 고혈압증의 역학적 연구 - 제2 보 : 고혈압증 1,017 예를 대상으로 본 한국인 고혈압증의 특징
손의석 ( E Suk Sohn ),강성효 ( Sueng Hyo Kang ),배종화 ( Jong Wha Bae ),윤재덕 ( Jai Duck Yoon ),박승철 ( Seung Chul Park ),이종석 ( Chong Suk Lee ),안승운 ( Seung Oon Ahn ),최일영 ( Il Young Choi ) 대한내과학회 1968 대한내과학회지 Vol.11 No.4
In the previous report the mean blood pressure, prevalence of hypertension and severity of hypertensive disease in Koreans were presented from the epidemiologic view point, The present observations were undertaken to evaluate, firstly, the various cliniaa1