관상동맥 스텐트 내 재협착의 발생에 미치는 임상적 관동맥 조영술적 요인들

윤혁준,김기식,박형섭,이영수,현대우,한성욱,허승호,김윤년,김권배,박남희,원경숙 대한순환기학회 2003 Korean Circulation Journal Vol.33 No.12

고유량 고효율 혈액투석막 재사용의 임상경험

조현경,김승정,임병국,정혁준,최영일,마경애,신규태,김흥수,김도헌 대한신장학회 2001 대한신장학회잡지 Vol.20 No.3

Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

Son, Ji Yeon,Kang, Yoon Jong,Kim, Kyeong Seok,Kim, Tae Hyung,Lim, Sung Kwang,Lim, Hyun Jung,Jeong, Tae Cheon,Choi, Dal Woong,Chung, Kyu Hyuck,Lee, Byung Mu,Kim, Hyung Sik Korean Society of ToxicologyKorea Environmental Mu 2014 Toxicological Research Vol.30 No.2

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

Ji Yeon Son,Yoon Jong Kang,Kyeong Seok Kim,Tae Hyung Kim,Sung Kwang Lim,Hyun Jung Lim,Tae Cheon Jeong,Dal Woong Choi,Kyu Hyuck Chung,Byung Mu Lee,Hyung Sik Kim 한국독성학회 2014 Toxicological Research Vol.30 No.2

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

Original Article : Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

( Ji Yeon Son ),( Yoon Jong Kang ),( Kyeong Seok Kim ),( Tae Hyung Kim ),( Sung Kwang Lim ),( Hyun Jung Lim ),( Tae Cheon Jeong ),( Dal Woong Choi ),( Kyu Hyuck Chung ),( Byung Mu Lee ),( Hyung Sik Ki 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

原乳에 過酸化水素의 添加가 T.T.C. Test에 미치는 影響

金義濟,李革新 건국대학교 1980 論文集 Vol.11 No.1

This experiment was carried out to investigate the effect of hydrogen peroxide added as a raw milk preservative on TTC test and titratable acidity. The results obtained were as follows; (1) The added amount of hydrogen peroxide less than 0.03% didn't effect on TTC test. (2) When the raw milk was stored at 20℃ after adding 0.05% hydrogen peroxide, the acidity was the same as that of raw milk until 21 hours later. (3) When the acidity of milk was more than 0.25%, it effected on TTC test. (4) When the milk was stored at 30℃ after adding 0.3% hydrogen peroxide, the acidity of milk was the same as the acidity of raw milk until 21 hours later. (5) In the case of adding 0.05% hydrogen peroxide, hydrogen peroxide was disappeared after 15 hours.

두경부 편평상피세포암 세포주의 VEGF 아형의 분포와 종양증식 및 이종이식성 : relation to xenotransplantability and tumor progression in mice.

김동욱,이종대,박진규,이재형,이병돈,장혁순 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.2

Overexpression of vascular endothelial growth factor(VEGF) is related to tumor progression and xenotransplantability in various human solid tumors, but the specific impact of the VEGF-subtypes is still under discussion. The aim of this study was to analyse a possible association of the major VEGF-isoforms and the growth characteristics of xenotransplanted human head and neck squamous cell carcinoma tumors in nude mice. Seven squamous cell carcinoma cell lines were analysed by quantitative RT-PCR using the Taqman^(TM)-System. We investigated the expression of VEGF-total-mRNA and of the major subtypes VEGF-121, -165, and -189 by using subtype specific primers. The cell lines were xenotransplanted in three mice each, and the data of tumor growth and progression were correlated to the expression of VEGF-isoforms. We also investigated an "growth response rate" measured by tumor growth per detected VEGF-level. Six out of the seven cell lines analysed expressed all isoforms of VEGF in different quantities. One cell line expressed generally low levels of VEGF and no VEGF-189 at all, In this cell line xenotransplantation failed in one mice out of three. In a second cell line transplantation failed in one out of seven mice, too. Success rates for the other five cell lines were 100%. The cell lines with higher transplantation success were expressing higher VEGF-121/165-189 ratios comparing to those without success. In contrast, linearity of tumor growth and lack of necrosis were associated with a lower VEGF-121/165-189 ratio. The findings demonstrate a predominant expression of VEGF-165 and VEGF-189, compared VEGF-121. In highly proliferating tumors this rate appeared to be about 10 times higher than in low proliferating tumors. We conclude that the ratio between the VEGF-subtypes during tumor implantation and growth is a prerequisite for progression and hypothesize an individual and different response of each tumor cell line to VEGF.

웨이블릿 변환과 GPS 정밀시각동기를 이용한 전력계통 고장점 모니터링 시스템에 관한 연구

김기택,김혁수,최정용 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.A

A continuous and reliable electrical energy supply is the objective of any power system operation. A transmission line is the part of the power system where faults are most likely to happen. This paler describes the use of wavelet transform for analyzing power system fault transients in order to determine the fault location. Synchronized sampling was made possible by precise time receivers based on GPS time reference, and the sampled data were analyzed using wavelet transform. This paper describes a fault location monitoring system and fault locating algorithm with GPS, DSP processor, and data acquisition board, and presents some experimental results and error analysis.

原乳의 冷却方法과 市乳의 殺菌方法 및 貯藏方法에 따른 細菌學的인 變化에 관한 硏究

金秀光,李革新 건국대학교 1979 論文集 Vol.9 No.1

The experiment was carried out to compare Total Standard Plate Counts (S.P.C) according to Cooling Method of Raw Milk and Pasteurization Method of Market Milk, to examine Changing of Market Milk at 5℃ and room temperature during various Storage Period. They were investigated in S.P.C. and quality of Markt Milk. The results obtained were as follows; 1.Cooling Methods of raw milk: S.P.C. were 4.7×107 cells/ml in cooling method by ground water, 3.2×107 cells/ml in refrigerator storage method, both were above the law. Then 13% of 4.7×107 cells/ml in milk cooler method and 75% of 1.9×106 cells/ml in bulk tank method were below the law. 2.Pasteurization methods of market milk; Raw milk contained 2.4×107 cells/ml, then M.T.S.T milk contain 2.6×105 cells/ml, both was above the law. H.T.S.T. and holding mixed method and L.T.L.T. method were contained 2.0×102 cells/ml and 1.3×102 cells/ml, respectively both were below the law, 6.2×10cells/ml by V.H.T. method was very excellent among them. 3.Storage of market milk at 5˚C; S market milk after 4th days was 2.6×103cells/ml in September, 3.2×102 cells/ml in October, both were below the law, K market milk after 4 th days in September was 6.0×104 cells/ml, after second day in October was 1.2×105 cells/ml, but in spite of pasteurization SA control market milk in September was 2.5×105 cells/ml, after firstday in October was 4.4×104 cells/ml, all were above the law. All samples were normal in Acidity test, Alcohol precipitation teat and Organoleptic test. 4.Storage of market milk at room temperature (23-25℃) ; S market milk after 12 hours storage in September was 1.8×105 cells/ml, and after 24 hours in October was 1.0×106 cells/ml. K market milk both in September and October after 8 hours were 1.3×105cells/ml and 8.4×104 cells/ml respectively SA market milk pasteurized immediately in September and in October were 2.0×105 cells/ml, and 2.6×105 cells/ml, and both were above the law. All of market milk were 0.18~0.25% in Acidity test, positive in Alcohol test, and abnormal flavor in Organoleptic test after 24 hours.

培地形態와 貯藏溫度 및 期間이 Str.lactis와 L.bulgaricus에 미치는 影響

金光洙,李革新 건국대학교 1979 論文集 Vol.9 No.1

The lactic acid bacteria were inoculated in original media as culture or washed cell form after 16 hours incubation in skimmilk or MRS broth and then production of lactic acid, etc. were investigated during 8 months frozen storage. The studies on the storage at low temperature after incubation in skimmilk and the use of additive (glycerol, skimmilk powder, lactose) to prevent injury of cells were carried out as accompanying treatments. The results obtained in this study were as follows. 1.The lactic acid production ability of L. bulgaricus after 8 months preservation at skimmilk medium was 0.72% in the culture treatment and 1.21% in washed cell treatment, but at MRS broth medium, it was 0.50% in culture treatment and 0.54% in washed cell treatment and shows the same trend at Str. lactis. It shows that skimmilk is better than MRS broth as a medium. 2.In washed cell treatment, the lactic acid production ability of Str. lactis after 8 months preservation was 0.26% in skimmilk medium and 0.57% in MRS broth medium, and in culture treatment it was 0.21% in skimmilk and 0.31 % in MRS broth. The same trend was shown in L, bulgaricus. 3.The activity of Str. lactis after 8 months frozen storage as washed cell form was better than of 18 days storage at 4℃. 4.In additive, the viability of lactic acid bacteria after 7 months preservation was 2.76×108 in glycerol 1.2×108 in skimmilk and 0.79×108 in lactose.