능성어류의 양식산업화를 위해서 (2) - 자바리의 어미관리와 종묘생산 -

이영돈,송영보,Lee, Yeong-Don,Song, Yeong-Bo 한국양식학회 2004 韓國養殖 Vol.16 No.2

한라산국립공원에 서식하는 딱 정벌레류의 시간적 분포

이영돈 ( Yeong Don Lee ),박성준 ( Seong Joon Park ),권용수 ( Yong Su Kwon ),김도성 ( Do Sung Kim ),김현철 ( Hyoun Chol Kim ),김동순 ( Dong Soon Kim ) 한국환경생태학회 2016 한국환경생태학회 학술대회지 Vol.2016 No.1

일반적으로 한라산과 같은 산지는 저지대에 비해 식생,지형 등의 자연환경이 고도와 경사에 따라 급격하게 변화하기 때문에 특정 환경에 적응한 동물들이 환경에 대한 다양한 특징을 나타내고 있다(Lomolino, 2001). 일반적으로 곤충은 환경조건에 따라 크게 영향을 받는 것으로 알려져 있으며(Kim, 1988), 특히, 딱정벌레류의 대부분 곤충들은 서식지 변화, 하층식생과 낙엽량, 숲의 나이와 토지 조건, 온도와 습도 변화 등의 환경변화에 매우 민감하여 산림의 형태에 따라 분포하는 종이 달라지기 때문에 고립된 상태 또는 제한된 공간 내에서 특정 종의 급격한 변화는 그 지역의 환경변화를 반영함으로 기후와 서식지 등의 변화를 분석할수 있는 환경지표성 곤충으로 인식되고 있다(Thiele, 1977; Maclean et al., 1992; Niemela et al., 1993; Ishitani and Yano, 1994; Butterfield et al., 1995; Ings and Hartley, 1999; Magura et al., 2000; Niemela et al., 2000; Koivula and Niemela 2002; Melnychuk et al., 2003; Rainio and Niemela, 2003; Argyropoulou et al., 2005; Ohsawa, 2005; Apigian et al., 2006; Avgin, 2006; Sandoval et al., 2007; .iogas and Vai.ikauskas, 2007; Timmetal., 2009; Rileyand Browne, 2011). 국내에서도 이러한 환경변화에 대한 딱정벌레 이용 연구의 중요성을 인식하여 고도, 식생 등 환경변화에 대한 종 다양도 및 풍부도 분석, 계절에 따른 개체군 변동, 지역적인 발생과 변화 등에 관한 연구가 수행되고 있다(Park et al., 1997; Jung et al., 2005; Yeon et al., 2005;Yang et al., 2006; Jung et al., 2011; Lee, 2011; Oh etal., 2011). 본 연구는 한라산국립공원에 서식하는 딱정벌레의 월별·계절별 분포 특성을 파악하기 위해 성판악(750m)에서부터 시작하여 정상인 백록담(1950m)과 어리목(970m)에 이르는 동서길이 약 18km구간에서 23개 조사구에 Pit-fall trap 을 설치하고 2010년 5월부터 10월까지 월 4회씩 설치 및 수거를 하였다. 조사결과 분포가 확인된 딱정벌레류는 총16과 71종(14,405개체)으로 딱정벌레과가 22종(30.99%)으로 가장 많고, 검정풍뎅이과 10종(14.08%), 송장벌레과 9종(12.68%), 잎벌레과와 소똥구리과 각 6종(8.45%), 방아벌레과 3종(4.23%) 등의 순으로 나타났다. 조사시기별 종 다양성은 6월에 43종으로 가장 많은 종이 출현하였고, 8월(32종), 7월(30종), 10월(25종), 9월(23종), 5월(21종) 순으로 나타났다. 출현 개체수는 10월 4,575개체, 9월 4,065개체순으로 많았고, 5월 561개체로 적었다. 군집분석 결과, 종다양도(H``)와 종균등도(EI)는 7월(H``=2.448, EI=0.720)이 가장 높고, 종풍부도(EI)는 6월에 5.229로 가장 높게 나타났다. 반면 종다양도와 종균등도는 10월(H``=0.695, EI=0.216), 풍부도는 9월(RI=2.647)에 가장 낮게 나타났다. 시기별 출현종을 분석한 결과 윤납작먼지벌레의 연간 2세대가 발생하는 종임을 재확인하였다. 또한 분류군에 따라 출현시기에 차이가 있는데 이는 한라산국립공원에 출현하는 딱정벌레목 곤충들 사이에 공간적, 시간적 출현과 먹이자원의 이용에 있어 서로 경쟁을 피하며 공존할 수 있는 여건이 형성되어 있음을 알 수 있었다. 본 연구결과는 아열대 기후로 인해 이국적이고 다양한 식생들이 수직 분포를 잘 나타내고 있고, 많은 기생화산들과 분화구 및 고산 습지와 고산초지대 등 다양한 환경으로 다른 공원에 비하여 동.식물들이 다양하고 풍부하며 자연환경도 비교적 잘 보전되어 있는 한라산국립공원지역에서 딱정벌레의 월별·계절별 분포특성을 조사하여 향후 환경변화에 대비한 한라산국립공원의 안정된 자연생태계를 유지 관리하는데 필요한 기초 자료로 제공하고자 한다.

김치에서 분리한 Lactococcus lactis W-44에 의한 감귤발효물의 양식 넙치 성장에 미치는 영향

김민수,문상욱,이영돈,김세재,김영진,이준원,이정희,이정숙,김보연,안종석,안순철,Kim, Min-Soo,Moon, Sang-Wook,Lee, Young-Don,Kim, Se-Jae,Kim, Yeong-Jin,Lee, Jun-Won,Lee, Jeong-Hee,Lee, Jung-Sook,Kim, Bo-Yeon,Ahn, Jong-Seog,Ahn, Soon-Cheo 한국미생물학회 2007 미생물학회지 Vol.43 No.2

젖산균을 접종하여 감귤 분쇄액을 발효시켜 얻은 감귤발효물의 어류양식 사료 첨가제로의 가능성을 넙치(Paralichthys olivaceus) 양식에서 검토하였다. 김치에서 항균활성이 우수한 젖산균 W-44를 분리한 후 16S rDNA 염기서열 분식을 통하여 Lactococcus lactis W-44로 동정하였다. L. lactis W-44를 이용하여 감귤 분쇄액 발효를 수행한 결과, 생리활성이 우수한 무배당체 flavonoids인 naringenin, hesperitin의 함량이 각각 약 10과 6배 증가함을 확인하였다. L. lactis W-44를 이용하여 발효된 감귤발효액을 양식넙치의 사료첨가제로 투여한 결과, 감귤발효액을 투여하지 않은 대조구의 평균 전장 및 체중 증가율과 현격한 차이가 있었다. 또한 0.2% (v/v)의 감귤발효액을 투여한 실험군에서 넙치의 성장이 가장 우수한 것으로 나타났다. 이러한 결과로부터 젖산균의 감귤발효산물을 넙치 양식에서 기능성 사료첨가제로 사용할 수 있는 가능성을 확인하였다. We evaluated the use of citrus fruit fermented by lactic acid bacteria, as a feed supplement for flounder (Paralichthys olivaceus) cultivation. For the fermentation, a lactic acid bacterial strain W-44 showing antibacterial activity was isolated from kimchi. From the phylogenetic analysis based on, 16S rDNA sequence, the strain W-44 was identified as Lactococcus lactis. After the fermentation of citrus fruit with L. lactis W-44, the contents of naringenin and hesperetin, bioactive flavonoid aglycones, were increased about ten-fold and six-fold, respectively. The effects of fermented citrus fruit-based feed additives (CFBFA) were tested on the growth of flounder, Paralichthys olivaceus. There were significant differences in average total length and body weight between the experimental and control group. The growth rate of the experimental group fed with the 0.2% CFBFA-supplemented diet was increased 4.5% and 20.9% more than the control group in total length and body weight, respectively. These results suggest that the fermented citrus fruit could be used as a functional feed additive for flounder cultivation.

리테르개멍게 (Halocynthia hilgendorfi ritteri)의 초기발생

최영진 ( Choe Yeong Jin ),김삼연 ( Kim Sam Yeon ),이치훈 ( Lee Chi Hun ),노섬 ( No Seom ),이영돈 ( Lee Yeong Don ) 한국수산과학회 2004 한국수산과학회지 Vol.37 No.2

N/A Early development and metamorphosis of the ascidian (Halocynthia hilgendorfi ritteri) were investigated from fertilized egg. The samples were collected in the coastal waters of Yongdam, northwest of Jeju Island in November 2002. H. hilgendor- ritteri was solitary ascidian and produced spheral eggs with egg size ranging from 0.33+0.01 mm. On the outer surface of the vitelline coat are attached many follicle cells. At 21.m0.5 c of water temperature, first cleavage took place in about 1.5 hrs after fertilization, and gastrulation followed in about 12.5 hrs. The formation of tailbud embryos and free swimming larvae were observed 13.3 hrs and 20.5 hrs after fertilization, respectively. The size of newly hatched tadpole larva was 1.30- 1.45 mm, the larva swam for 2 hrs to 14 hrs. At 4 hrs after hatching, the palpi were lost and tail absorption began with an abrupt rupture of the anterior end of the notochord. At 17-18 hrs after hatching, tail completely absorption and remained trunk. The coniform adhesive papilla began protrusion at 30 hrs after hatching. The oral and atrial siphon formed at 6-7 days after settlement. At 17-18 days after settlement, metamorphosed the larvae developed into protoascidian of which the external morphology was similar to their adult.

소아의 서혜부탈장 양측성 및 일측탈장술후 대측탈장 빈도

정주광(Ju Kwang Chung),이영돈(Yeong Don Lee),이태훈(Tae Hoon Lee) 대한외과학회 1991 Annals of Surgical Treatment and Research Vol.40 No.2

붉바리(Epinephelus akaara) 기형 발생 치어의 형태형성 유전자 발현

유진호,문성희,오현지,백혜자,이영돈,이치훈,권준영,You, Jin Ho,Mun, Seong Hee,Oh, Hyeon Ji,Baek, Hea Ja,Lee, Young-Don,Lee, Chi Hoon,Kwon, Joon Yeong 한국해양생명과학회 2019 한국해양생명과학회지 Vol.4 No.1

붉바리(Epinephelus akaara) 종자생산 시 기형 발생에 의한 손실이 크지만 기형어에 대한 생물학적 정보는 많지 않다. 본 연구에서는 부화 후 96일 붉바리 치어를 정상 그룹과 두 유형의 기형 그룹(머리, 턱)으로 나누어 형태형성과 연관된 4개의 주요 유전자(insulin like growth factor 1: IGF-1, bone morphogenic protein 4: BMP4, peroxisome proliferator-activated receptors γ: PPARγ, matrix Gla protein: MGP) 발현을 조사하였다. 각 그룹에서 뇌, 간 및 근육을 잘라낸 다음 total RNA를 추출한 후 real-time PCR을 사용하여 유전자 발현 차이를 비교하였다(n=20). 부화 후 96일 붉바리 치어에서 IGF-1과 BMP4 유전자는 기형 그룹의 뇌와 간에서 정상 그룹과 비교하여 유의한 발현 차이를 나타냈다(p<0.05). 반면에 PPARγ와 MGP 유전자는 어떤 조직에서도 정상 그룹과 기형 그룹 사이에 유의한 발현 차이를 보이지 않았다. IGF-1과 BMP4 유전자는 치어 단계의 붉바리 기형 상태와 관련되어 있는 것으로 보인다. The deformity occurring at the early developmental stage of red spotted grouper (Epinephelus akaara) causes detrimental effects on the process of juvenile production. In this study, we have compared the expressions of several key genes (insulin like growth factor 1: IGF-1, bone morphogenic protein 4: BMP4, peroxisome proliferator-activated receptors γ: PPARγ, matrix Gla protein: MGP) for morphogenesis between normal and 2 types (cephalic and jaw) of deformed juvenile fish. Expression of these genes were investigated in the brain, liver and muscle of each group of fish (n=20) by real-time PCR. Expression of IGF-1 and BMP4 mRNA in the brain and liver showed significant difference between normal and deformed fish (p<0.05). However, no difference was observed in the expression of PPARγ and MGP mRNA between normal and deformed fish in any tissues. It seems certain that IGF-1 and BMP4 are associated with the state of deformity in juvenile red spotted grouper.

유두상 갑상선암의 수술 시 예방적 중앙경부림프절 절제술을 통해서 살펴본 림프절 전이의 양상

박원종,김혜경,한상훈,이영돈,Won Jong Park,M.D.,Hye Kyoung Kim,M.D.,Sang Hoon Han,M.D. and Yeong Don Lee,M.D. 대한갑상선-내분비외과학회 2005 The Koreran journal of Endocrine Surgery Vol.5 No.1

Purpose: In papillary thyroid carcinoma, lymph node metastasis at presentation does not seem to affect survival adversely, but does increase the risk of loco-regional tumor recurrence. The benefits of prophylactic central neck node dissection (PCND) have not been clearly demonstrated so far and should be weighed against the potential risks of the procedure. The aims of this study are as follows; to determine the frequency and the pattern of the lymph node metastasis and the risk factors influencing the metastasis to level VI lymph nodes, and to determine the necessity of the contralateral central lymph node dissection. Methods: A retrospective analysis had been carefully performed over 68 patients with papillary carcinomas undergoing total thyroidectomy with PCND during the period from July 1, 2000 to August 31, 2002. In addition to the analyses of the incidence and the pattern of central lymph node metastasis, we statistically analyzed the correlation between lymph node metastases and the risk factors such as tumor size, age, lymphatic tumor emboli, vascular tumor emboli, perithyroidal soft tissue invasion, and multifocality, etc. Results: The mean age was 46 years (22∼76) and the tumor size ranged from 0.2 to 9 cm (mean 2.2 cm). The micropapillary carcinoma was detected by pathological findings in 10 patients (14.7%). The lymphatic tumor emboli and vascular tumor emboli occurred in 8 patients (11.8%) and 2 patients (2.9%) respectively. Thirty eight patients (55.9%) showed perithyroidal tissue invasion. The mean number of harvested lymph nodes in level VI was 13.7 (6∼32). Among 68 patients, lymph node metastasis occurred in 49 patients (72.1%) and the mean number of metastatic lymph node was 5.4. Among the patients with metastasis, the rate of metastasis among the harvest nodes amounted to 39.0%. Metastasis to Delphian nodes was detected in 2 patients (2.9%). In addition, 15 patients (22.1%) showed metastatic contralateral central nodes. Patients less than 40 years old tend to have more metastatic lymph nodes (P=0.012). Futhermore, the patients with tumor larger than 2 cm increased incidence of lymph node metastasis (P=0.036). Conclusion: After the prophylactic central neck nodes dissection in case of papillary carcinoma patients, metastatic lymph nodes were found in 72.1%. In conclusion, we would like to recommend prophylactic central neck node dissection to papillary carcinoma patients in order not only to prevent local recurrence but to avoid the difficulties of reoperation, especially for those with high risk for nodal involvement (less than 40 years old or more than 2 cm of tumor size). Because of the relatively high incidence of contralateral central lymph nodes metastasis, we suggest the importance of dissection of contralateral central lymph nodes along with unilateral central. (Korean J Endocrine Surg 2005;5:7-11)

충수돌기에 발생한 점액낭종

박승연(Seung Yeon Park),이정남(Jung Nam Lee),정민(Min Chung),이운기(Un Gi Lee),이영돈(Yeong Don Lee),오재환(Jae Hwan Oho),백정흠(Jung Hum Bak),박연호(Yeon Ho Park),김현영(Hyeon Yeong Kim),김건국(Keon Kuk Kim),최상태(Sang Tae Choi) 대한외과학회 2007 Annals of Surgical Treatment and Research(ASRT) Vol.72 No.2

Appendiceal mucocele is a cystic dilatation of the appendiceal lumen with mucus, which may be caused by either benign or malignant diseases. In this report, five cases of appendiceal mucocele are reported, three of which had a preoperative diagnosis of mucocele, whereas the others were found incidentally during the operation under the diagnosis of intussusception and periappendiceal abscess. All five patients underwent surgical resections, including one ileocecal resection and four right hemicolectomies. Histopathology revealed a mucinous cystadenoma in four cases, and a mucinous cystadenocarcinoma in one. There was no disease-related death during 18 months of follow-up.

복부 자상의 치료 방법에의 접근

박지연 ( Ji Yeon Park ),정민 ( Min Chung ),이영돈 ( Yeong Don Lee ),이정남 ( Jung Nam Lee ),이운기 ( Woon Ki Lee ),박연호 ( Yeon Ho Park ),백정흠 ( Jung Heum Baek ),박흥규 ( Heung Gyu Park ),김건국 ( Keon Kuk Kim ),강진모 ( Jin M 대한외상학회 2010 大韓外傷學會誌 Vol.23 No.2

Purpose: A classic approach to abdominal stab wounds has been a routine laparotomy for the purpose of diagnosis or treatment. However, management protocols for abdominal stab wounds are still contentious in most trauma centers. We examined the relationship between the character of the stab wound and the injured intraabdominal organs by retrospectively analyzing the medical records of patients with abdominal stab wounds admitted to Gil hospital, and the findings for our patients are then confronted with a review of the literature. We aimed to propose proper management protocols to approach abdominal stab wounds. Methods: The medical records of all 80 patients sustaining abdominal stab wounds, admitted at the Department of Surgery, Gil Hospital, Gachon Medical School, from January 2004 to December 2008 were retrospectively reviewed. All the abdominal stab wounds were collated based on the site and the character of the injury, investigations performed on admission, results of investigations, operations performed and findings at the time of the operation. Results: The most prevalent age group was patients in their forties and the average age of the patients was 41 years for both genders. The stab wounds were most commonly located at the periumbilical area (16.9%), followed by the epigastric area (15.6%), and 18.2% of the patients had multiple wounds. The most commonly eviscerated organ was the omentum (9 out of 16 cases); 61.7% of non-eviscerated patients underwent a therapeutic laparotomy while 81.3% of eviscerated patients underwent a therapeutic laparotomy. The small bowel was the most commonly injured organ (22.7%, 17 out of 75 injuries). The review revealed a relatively common diaphragmatic injury in abdominal stab wound patients (8 cases, 10.5%). The average hospital stay was 11 days. Conclusion: This review revealed commonly eviscerated and injured intraabdominal organs in abdominal stab wound patients and their relationship with a therapeutic laparotomy. Although the management is still controversial, the authors suggest indications for an immediate laparotomy and a protocol for managing abdominal stab wounds. Hemodynamic instability and peritoneal irritation signs are definite indicators for an immediate laparotomy, but the review revealed intraabdominal organ evisceration alone not to be a statistically significant factor. In addition, the authors suggest that abnormal CT findings can be valuable for making a decision on management of hemodynamically stable stab wound patients. Further study may clarify a role for a more selective approach to operative intervention and for a more extensive use of selective observation. (J Korean Soc Traumatol 2010;23:134-141)

한국산 멸종위기종 산굴뚝나비(나비목, 네발나비과)의 분포와 개체군 동태

김도성 ( Do Sung Kim ),조영복 ( Young Bok Cho ),김동순 ( Dong Soon Kim ),이영돈 ( Yeong Don Lee ),박성준 ( Seong Joon Park ),안능호 ( Nung Ho Ahn ) 한국환경생태학회 2014 한국환경생태학회지 Vol.28 No.5

본 연구는 제주도 한라산에 서식하는 산굴뚝나비의 분포와 개체군 동태를 알아보기 위하여 선조사법과 포획-재포획 조사법을 실시하였다. 그 결과 산굴뚝나비는 해발 1500m 이상부터 관찰되기 시작하여 정상부까지 서식하는 것으로 확인되었다. 포획-재포획 조사에서 산굴뚝나비의 포획 개체수는 1,493개체로, 이중 수컷은 978개체, 암컷 515개체가 확인되었다. 재포획된 개체수는 518개체이며, 수컷과 암컷의 비율은 284:234로 나타났다. 그리고 암컷과 수컷의 평균생존일수는 2.31로 나타났으며, 이중 수컷 2.14일, 암컷 3.47로 나타나 암컷이 수컷 보다 오래 생존한 것으로 나타났다. 포획-재포획 조사를 통한 일일 추정개체수는 수컷이 7월에 약 1000개체를 유지하다가 점차 감소하는 것으로 나타나고 있으며 8월에는 개체수가 200개체 이하로 나타났다. 그리고 암컷은 7월에 335개체를 최고로 하였다가 점차 감소하고, 이후 8월에 이르러 개체군 크기는 120개체 이하로 나타났다. 추정개체수의 크기는 암컷이 수컷의 약 1/3수준으로 나타났다. 산굴뚝나비의 평균 이동거리는 수컷 116.8±191.9m, 암컷 118.4±161.5m로 나타나 암수간의 차이는 거의 없었다. 산굴뚝나비는 한라산 백록담을 중심으로 넓게 형성된 초지공간에서 단일 개체군을 이루고 있다. 개체 밀도가 가장 높은 곳은 훼손지 복구지역으로, 이는 한라산의 훼손된 지역을 복구하는 작업과정에서 먹이식물인 김의털이 넓은 면적으로 자라고 있어 이 지역을 중심으로 많은 개체들이 서식하고 있는 것으로 나타났다. This study was conducted to investigate the distribution and the population dynamics of Hipparchia autonoe by using a line transect and Mark-Release-Recapture (MRR) at the Mt. Halla in Jeju Island. The results showed that H. autonoe was found from 1,500 m above the sea level. Total 1,493 H. autonoe with 978 males and 515 females were captured and released in the MRR study site. Among them, 518 individuals including 284 males and 234 females were recaptured. The average survival time was 2.31 days with 2.14 days for males and 3.47 days for females, indicating longer survival time in case of females than males. The daily population size of males estimated in the MRR study site was maintained about 1,000 individuals in July and gradually decreased less than 200 in August. The number of females showed peak at 335 individuals on July 24, and gradually decreased less than 120 in August. Thus, female population was 1/3 of males. The average travel distance of male and female H. autonoe were 116.8 ± 191.9 m and 118.4 ± 161.5 m, respectively, indicating almost same between sexes. H. autonoe in the Mt. Halla formed single population group in the wide meadow around the Baekrokdam Lake. The highest population density of H. autonoe was occurred in the restored area from damages, where host plants such as the sheep``s fescue or the food plant are abundant by artificial restoration efforts.