충치균에 대한 생리활성 생약성분의 분리 및 약효평가(1) : 튜립나무잎의 항균성 성분과 안전성에 대하여 On the Antibacterial Component of the Leaves of Liriodendron tulipifera and Its Safety

배기환,김봉희,명평근,변재화 충남대학교 약학대학 의약품개발연구소 1990 藥學論文集 Vol.6 No.-

The isolation and identification of an antibacterial component, from the leaves of Liriodendron tulipifera. K. Kotch against a cariogenic bacterium Streptococcus mutans OMZ 176, were carried out for developing of anticariogenic agents. The bioactive component was elucidated as β-liriodenolide, which was isolated newly from the leaves of L.tulipifera. The minimal inhibitory concentration (MIC) of β-liriodenolide was 100 ㎍/㎖ and the antibacterial activity was stronger than that of berberine. β-Liriodenolide inhibited β-lactamase activity, 50. 100 and 200 μM β-liriodenolide did β-lactamase activity as 0.7, 3.5 and 19.7%, respectively. The toxicity of β-liriodenolide was not found with the method of photohemolysis.

은행잎 플라보놀배당체에 대한 셀루라제류의 영향

배기환,민병선,백흠영,안병준 충남대학교 암연구소 1991 癌共同硏究所 硏究誌 Vol.1 No.1

The extractability and stability of ginkgoflavonolglycosides under presence of several cellulase preparations were investigated. The enzymes used were macerosin, cellulase C and cellulase NC. The content variation of the glycosides was measured with HPLC method, using caffeic acid as an internal standard. The methanol extract of ginkgo leaf, containing the total flavonolglyco-sides of 4.46%, was used for the content comparison. By extraction with the enzymes, each or mixed, the peak levels of all the glycosides began to decrease after 1 or 2 hours. After 24 hour extraction, most of the glycosides were degraded to minor components. The flavonolglycosides in ginkgo leaf were also hydrolysed simply by the water extraction. After 24 hour extraction with water at 40°C, the peak levels of major glycosides were distinctly decreased. Rutin was hydrolysed by enzyme treatment or by ginkgo leaf itself. As a result, it was concluded that the commercially available cellulases and the ginkgo leaf itself contain the activities of β-glycosidase and α-rhamnosi-dase. Kaempferol-3-0-(6' -0-p-coumaroylglucosyl)-rhamnoside and four other ginkgo flavonolglycosides were not hydrolysed under the same condition."

7,8-Dioxo-A-norerythrinan의 합성

배기환,서원준 충남대학교 약학대학 의약품개발연구소 1994 藥學論文集 Vol.10 No.-

7,8-Dioxo-A-norerythrinan (A) was synthesized from acid catalyzed cyclization of 6a-hydroxy-1-(2-phenylethyl)-octahydrocyclo-penta[b]pyrrole-3a-caboxylic acid ethyl ester (B) with concomitant deethoxycarbonylation. The intermediate (B) was a hydroxylated compound of N-acyliminium (C). The unstable pyrrolinium (C) was prepared from oxalylation of the enamine of phenethylamine and ethyl 2-oxocyclopentanecarboxylate.

棉實의 筋收縮, 血壓降下 및 過血糖作用

배기환 충남대학교 자연과학연구소 1984 忠南科學硏究誌 Vol.11 No.1

The purpose of this study was to investigate the action of water extract of Gossypium Semen(GWE). It was clarified that GWE caused the contraction of an isolated frog's rectus abdominis muscle, the fall of the blood pressure in a rabbit, and the hyperglycemia in a hen.

4,4'-Biphenol 유도체의 합성 및 충치균 Streptococcus mutans OMZ 176에 대한 항균작용

배기환,서원준,임승희 충남대학교 약학대학 의약품개발연구소 1992 藥學論文集 Vol.8 No.-

In a continuous study for the developing of the anticariogenic agents, 3,3'-diacyl-4,4'-biphenol derivatives (Fig.2, 2 and 3) and 3,3'-bis-(l-hydroxyalkyl)-4,4'-biphenol (Fig.3, 4 and 5) derivatives are synthesized successively from 4,4'-diphenol (Fig.2, l). The synthesized compounds are tested for their antibacterial activity against a cariogenic bacterium, Streptococcus mutans OMZ 176. The acyl derivatives, 2 and 3, do not show antibacterial activity, but the hydroxyalkyl derivatives, 4 and 5, reduced from the acyl group of 2 and 3, show the activity. The antibacterial activity of 2 and 3 may be inhibited due to intramolecular hydrogen bonding between the acyl group and the hydroxyl one (Fig.4).

대추로부터 베튜리닉 산과 알피톨릭 산의 분리 및 정량

배기환,이상명,이은실,이준성,강종성 충남대학교 약학대학 의약품개발연구소 1996 藥學論文集 Vol.12 No.-

Betulinic acid and alphitolic acid, the triterpenoids of Zyziphi Fructus, were isolated with silica gel column chromatography and used as the standard substances for the analysis. The compounds were determined with HPLC and HPTLC. They were separated on reversed phase coumn (Nova-Pak C_18) with 0.05 M Na_2HPO_4-methanol (19:81) in HPLC and detected at 210 ㎚. Separation on HPTLC precoated sillica gel F_254 plates was carried out with chloroform-methanol (6:1) and the separated compounds were reacted with ρ-anisaldehyde and detected at 540 ㎚. The contents of betulinic acid and alphitolic acid in Zyziphi Fructus from four different regions in Korea were in the range of 2.9~3.8% and 3.2~3.9%, respectively.

포도 Muscat Bailey A의 無核果生産을 위한 Gibberellin 의 處理試驗(1)

裵基煥 진주산업대학교 1983 論文集 Vol.21 No.-

포도 Muscat Bailey A 品種의 無核果生産을 위한 Gibberellin의 前處理 適期를 모색코저 本 試驗이 試圖되었다. 生育이 良好한 17年樹 供試하여 後處理는 滿開 後 8日에 모든 處理區를 同一히 하고, 前處理時期는 滿開 前 14日(G_1區), 12日(G_2區), 10日(G_3區), 8日의 處理區(G_4區)를 두어, 前後 處理 共히 GA_3 100 ppm 濃度로서 果房을 浸漬處理하여 그 處理가 果房에 미치는 影響을 調査하였던 바, 다음과 같은 結果를 얻었다. 1. 熟期는 모든 處理區가 無處理區에 비하여 約 20日 旱熟되었다. 2. 無核粒의 比率은 處理區가 無處理區에 비하여 有意的으로 높으나, 處理區라 할지라도 有核粒의 含有率이 높아, 無核果生産을 위한 滿足한 結果를 얻지 못하였다. 3. 果房長은 G_4 區를 除外한 그외의 處理區는 無處理區에 비하여 有意的으로 길었다. 4. 果房重은 無處理區에 비하여 G_2 및 G_3 區만이 有意的으로 무거웠고, G_1 및 G_4區는 有意差가 없었다. 5. 着粒數는 處理區라 할지라도 無處理區에 비하여 有意性이 없었다. 6. 平均果粒重은 處理區가 無處理區에 비하여 有意的으로 무거웠다. 7. 果粒의 均一度는 處理로 因한 有意性이 없었다. 8. 果粒의 密着度는 無處理區에 비하여 G_3 및 G_4區는 有意的으로 密着되나, G_1 및 G_2區는 有意性이 없었다. In order to find the best time of gibberellin application for seedless berry production in Muscat Bailey A grapes, this study was designed. The 17­year­old trees grafted on Teleki No. 8B rootstocks were selected as test trees. GA_3 at 100 ppm concentration was applied twice before and after full bloom by dipping the clusters. The before applications were carried out on different days according to designed treatments, and all the after application on the 8th day after the full bloom. The designed treatments were the the following 4 kinds : G_1 treatment applied on the 14th day, G_2 treatment on the 12th day, G_3 treatment on the 10th day, G_4 treatment on the 8th day before the full bloom.The results obtained through all the treatments were not satisfiable for seedless berry production, but the maturity in all the treatments were hastened by about 20 days compared with in the untreatment. Therefore, these treatments showed the possibility to early mature cultivation. The results of this study suggested that the practical before application period for early mature cultivation was within the range of the 12th day to the 10th day before full bloom.

L1210 세포에 대한 약용 식물의 세포독성 검색

배기환,민병선,도동선,김남수,양기종,안병준 충남대학교 약학대학 의약품개발연구소 1992 藥學論文集 Vol.8 No.-

For the research of cytotoxic natural products, 50 medicinal plants were extracted with benzene and methanol, separately, and screened against L1210 cells. From the results(Table I), 6 samples showed cytotoxicity both in benzene and methanol extracts of 17 samples in benzene extracts and 3 samples in methanol extracts, respectively. Generally, the cytotoxicity exhibited high frequency (34%) in benzene extract but low frequency in methanol extract(6%), it meant that active cytotoxic components had less polarity. ED_50 values less than 10 ㎍/㎖ were observed in 17 medicinal plants.

晋州地方의 배 開化期 調査

裵基煥 진주산업대학교 1984 論文集 Vol.22 No.-

長十郞 外 11品種에 대한 晋州地方에서의 開花期를 1981年부터 1984年까지 4年間에 걸쳐 調 査하였는데, 그 結果는 다음과 같았다. 1. 開花期(開花始期∼落花盛期)는 調査年度와 品種에 따라 4月 7日부터 5月2日 사이에 있었고, 滿開期는 4月 10日부터 4月 26日 사이에 있었다. 2. 品種別 滿開期의 早晩은 長十郞보다 이른 것…今村秋 長十郞과 같거나 前後하는 것…단배, 新水, 豊水, 二十世紀, 君塚旱生 長十郞보다 늦은 것…菊水, 雲井, 新世紀, 幸水, 晩三吉. 3. 品種別 開花期間은 8∼14日間인데, 大部分의 品種이 10∼11日 間이었다. 4. 해에 따른 開花期의 早晩은 3月의 月平均 氣溫과 密接한 關係가 있다. The author investigated on blossom time of pear in Jinju region with 12 cultivars over four years from 1981 to 1984. The results of this investigation were summarized as follows: 1. The blossom time was from April 7 to May 2 and the full bloom time was from April 10 to April 26 according to years and cultovars. 2. The fullbloom time of Imamuraaki was the earlist in all cultivars investigated and those of Okusankichi, Kosui. Sinseiki, Gumoi, and Gikusui were always late compared with that of Chojuro. 3. The blossom period of the most cultivars 10∼11 days in general, but that of Imamuraaki was 8∼10 days and Nigiiseiki 11∼14 days accordig to years. 4. The earliness and lateness of blossom time was closely correlated with month average temperature of March.

생약으로부터 충치예방 및 치료제 개발

배기환 영남대학교 약품개발연구소 1993 영남대학교 약품개발연구소 연구업적집 Vol.3 No.-

One hundred and fifty kinds of crude drugs were tested for their antibacterial against a main cariogenic bacterium. Streptococcus mutants OMZ176. Among the crude drugs, the methanolic extracts of Magnolia ovovata, Liriodendron tulipifera, Reynoutria elliptica, and Commelina communis showed strong inhibitory activaty on the growh of S. mutans. The fractonation of the extracts, monitored with the bioassay, resulted in the isolation of the active components. Magnolol and honokiol were isolated from harman and nonharman from C. communis. The chemical structure and activity relationships are also discussed.