단신 : 반응표면분석법을 이용한 Arthrobacter sp. PAMC 25486의 카로티노이드 생산배지 최적화

김현도 ( Hyun Do Kim ),최종일 ( Jong Il Choi ),한세종 ( Se Jong Han ) 한국화학공학회 2014 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.52 No.6

본 논문에서는 반응표면분석법을 이용한 Arthrobacter sp. PAMC 25486의 carotenoids 생산 배지의 최적화를 수행하였다. Placket-Burman 방법을 이용하여 yeast extract, MgSO4, dextrose가 carotenoids의 생산에 영향을 미치는 주요인자인 것을 확인하였다. 반응표면분석 방법을 이용하여 최대 carotenoids 생산 농도를 갖는 yeast extract, MgSO4, dextrose의 농도를 계산한 결과 1 g/L yeast extract, 0.0879 g/L MgSO4 and 1 g/L dextrose의 농도에서 최대 307 mg/L 의 carotenoids 농도가 예측됐으며, 실제 배양 결과 288 mg/L carotenoids가 얻어졌다. 얻어진 농도 값은 최적화 이전의 값에 비하여 200% 이상 증가하였다. 이러한 결과로부터 미생물 배양에 의한 carotenoids 생산을 증가시키기 위한 배지최적화 방법으로서 반응표면분석법의 유용성을 확인할 수 있었다. This study was conducted to optimize the medium composition for carotenoid production in Arthrobacter sp. PAMC 25486 through response surface methodology (RSM). Using a Placket-Burman design, from which yeast extract, MgSO4 and dextrose were identified as the significant factors affecting carotenoids production. RSM studies for carotenoids production by Arthrobacter sp. PAMC 25486 have been carried out for three parameters of yeast extract, MgSO4 and dextrose concentrations. These significant factors were optimized by experiments and RSM, as 1 g/L yeast extract, 0.0879 g/L MgSO4 and 1 g/L dextrose. The experimentally obtained concentration of carotenoid was 288 mg/L, and it became 2-fold increase on concentration before optimization.

아그로박테리움을 이용한 Actinobacillus pleuropneumoniae ApxIIA (ApxII toxin) 유전자 발현 옥수수 형질전환체 개발

김현아,유한상,양문식,권석윤,김진석,최필선,Kim, Hyun-A,Yoo, Han-Sang,Yang, Moon-Sik,Kwon, Suk-Yoon,Kim, Jin-Seog,Choi, Pil-Son 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.3

To develop edible vaccines for swine, the embryogenic calli (type II) derived from HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vector pMYV611, 613, 616, and V621, 622 and 623 respectively. Six of those vectors carry nptII gene which confers resistance to paromomycin and apxIIA gene producing ApxII toxin which is generated in various serum types of A. pleuropneumoniae as a target gene. The 4,120 callus clones for pMYV611, 5,959 callus clones for pMYV613, 7,581 callus clones for pMYV616, 52,329 callus clones for V621, 48,948 callus clones for V622, and 56,188 callus clones for V623 were inoculated. The frequency of positive response clone was confirmed into range of 2.3% - 4.4% for each vectors by NPTII ELISA kit assay, and the selected callus clones of them were finally 3 callus clones from pMYV611 (0.07%), 4 callus clones from pMYV613 (0.07%), 2 callus clones from pMYV616 (0.03%), 51 callus clones from V621 (0.1%), 72 callus clones from V622 (0.15%), and 102 callus clones from V623 (0.18%) respectively. From the selected callus clones of each binary vector, the integration of the apxIIA gene into maize genome was detected from 2 plants of pMYV613 and 2 plants of V623 by Southern blot analysis. 돼지 흉막폐렴백신을 개발하기 위해 옥수수 HiII genotype 으로부터 유도한 type II형의 배발생캘러스를 식물발현벡터 pMYV611, pMYV613, pMYV616, V621, V622 및 V623로 형질전환시킨 Agrobacterium (C58C1)과 공동배양 하였다. 이들 식물발현벡터는 paromomycin 항생제 저항 유전자인 NPTII 선발마커와 표적 유전자로서 흉막폐렴균의 여러 가지 혈청을 생산하는 apxIIA유전자로 재조합하여 구축하였다. 식물발현벡터pMYV611, pMYV613, pMYV616, V621, V622 및V623의 경우 각각 4,120개, 5,959개, 7,581개, 52,329개, 48,948개 및 56,188개의 캘러스 클론을 Agrobacterium과 공동한 후 NPTII assay kit에 의해 nptII유전자의 발현빈도를 조사한 결과 각 벡터별로 2.3-4.4%의 캘러스 클론에서 항체결합 양성반응을 보였고, 이들 중 최종적으로 선발된 형질전환 캘러스 클론은 pMYV611에서 3개 (0.07%), pMYV613에서 4개 (0.07%), pMYV616에서 2개 (0.02%), V621에서 51개 (0.1%), V622에서 72개 (0.15%) 및 V623에서 102개 (0.18%)를 각각 얻었다. 형질전환된 캘러스 클론으로부터 재분화된 식물체에서 유전자 도입여부를 Southern 분석으로 통해 확인한 결과 pMYV613에서 2개 식물체 및 V623에서 얻은 2개 식물체에서 각각 확인되었다.

선천성 식도 폐쇄증의 예후 판정

김현학,정현아,김경헌,김정한,이석구,Kim, Hyun-Hahk,Jeong, Hyeon-A,Kim, Kyung-Hun,Kim, Jung-Han,Lee, Suk-Koo 대한소아외과학회 2001 소아외과 Vol.7 No.1

The aims of this study were to evaluate the prognostic predictabilities of the risk classifications (Waterston, Montreal, and Spitz), preoperative treatment of pneumonia and the length of esophageal gap in esophageal atresia. Twenty-five cases of esophageal atresia were reviewed retrospectively. The data was analyzed with Likelihood ratio test for trend and Fisher's exact test. Survival rates were 100.0 % in group A and B, and 60.0 % in group C according to Waters ton classification (p=0.027). By Montreal classification, 95.7 % in group I and 50.0 % in group II (p=0.086). By Spitz classification, 95.5 % in group I and 66.7% in group II (p=0.159). The survival rate was 85.7 % in the patients with pneumonia preoperatively treated and 94.4% in the patients without pneumonia. The difference was not significant (p=0.490). Survival rate in the patients with long gap (${\geq}$3cm) was 50.0 % and 100.0 % in patients with short gap (<3cm) with significant difference (p=0.020). The Waterston classification could be modified into two groups because the prognosis of both group A and B were the same. Preoperative pneumonia does not seem to be a risk factor. The length of the esophageal gap showed prognostic value.

발전량 비교를 통한 창조식 조력발전의 기동낙차 결정에 관한 연구

김현한(Hyun-Han Kim),김광호(Kwang-Ho Kim) 대한전기학회 2018 전기학회논문지 Vol.67 No.5

Because of its predictability of the energy cycle and huge scale power output, the ocean energy from tidal power utilization has always received attention as a great energy source, even though its development cost, including the embankment construction, is so much higher than that of any other energy source. Nevertheless, nowadays many projects are being planned on account of institutional support from the government and the recent advance of construction technology. In Korea, the new industry field operating and managing the tidal power plant has already opened. But we are facing a number of problems for optimal operation of tidal power plant that are a lack of operation experience and a skill of professional management and others. This paper suggests a novel way to determine the starting head of power generation by generating power comparison method For this new method, the paper discusses many factors including changing the volume of the basin, the number of operating turbines and gates and forecasting the tidal amplitude and the characteristic curve of turbine and gate. Finally we verified that it can increase about 2% an annual power generation compared with the conventional method using the original operational function made in the plant design process.

배전계통에 연계된 풍력발전기 운영특성 분석

김현한(Hyun-Han Kim),김광호(Kwang-Ho Kim),전정표(Jung-Pou Jun),옥연호(Yeon-Ho Ok),곽희진(Hee-Jin Kwak),장진승(Jin-Seung Jang) 대한전기학회 2011 대한전기학회 학술대회 논문집 Vol.2011 No.7

수상태양광 발전시스템의 운영특성 및 설계요소에 관한 연구

김현한(Hyun-Han Kim),김광호(Kwang-Ho Kim) 대한전기학회 2017 전기학회논문지 Vol.66 No.10

The floating photovoltaic system is a new concept in the renewable energy technology. That is similar to land based photovoltaic technology except floating system. So the system needs buoyant objects, mooring, ect, besides modules and supports, and that is able to withstand in water level changes and wind strength. Therefore the floating photovoltaic system is much different from land photovoltaic system. K-water (Korea Water Resources Corporation) has been operating two floating photovoltaic system that"s capacity is 100 ㎾ and 500 ㎾ respectively since in summer 2011 for commercial generation, and have construction project for 2,000 ㎾ in Boryeong multipurpose Dam and other areas. Furthermore K-water was developing a tracking-type floating photovoltaic system at Daecheong multipurpose Dam and developed and installed an ocean floating photovoltaic demonstration plant at Sihwa Lake in October 2013 for R&D. In this paper, we introduce that structure of floating photovoltaic system include buoyant structure, mooring system and auxiliary device. Especially the rope which is in part of mooring should be always maintain tension under any water level. Also we explain about structure design concept to wind load in an every loading condition and a kind of structure materials and PV structure types used in water environment. Especially ocean floating PV system is affected by tidal current and typhoon. So there are considering the elements in design. Finally we compare with floating and land photovoltaic on power amount. As a result of that we verified the floating photovoltaic system is more about 6.6~14.2 % efficiency than a general land photovoltaic system.

조력발전소 조위 제약사항을 고려한 발전기 효율시험에 관한 연구

김현한,전정표,김광호,Kim, Hyun-Han,Jun, Jung-Pou,Kim, Kwang-Ho 강원대학교 산업기술연구소 2013 産業技術硏究 Vol.33 No.1

Tidal power generation is to produce electrical energy from fluctuations of the ebb and flow in a constructed embankment. More specifically, the sinusoidal variations of tidal flow, along with the periodicity and changes in the height of waves over time make the tidal power generation possible at a certain tidal level. This paper proposes a more practical efficiency test method for tidal power plant generator that utilizes the axis torque changing values rather than the retardation method which is commonly used. The proposed method was compared with the conventional method and the test result shows that proposed method provides a similar accuracy with the conventional retardation method and a better efficiency.

전산해석을 이용한 가스센서 설치위치에 따른 응답시간에 관한 연구

김현일(Hyun-il Kim),김영두(Youngdoo Kim),박규태(Gyou Tae Park),한상인(Sangin Han),권정락(Jeongrock Kwon) 대한전기학회 2008 대한전기학회 학술대회 논문집 Vol.2008 No.10

현재 가정이나 산업용으로 사용하는 가스 화기나 보일러 등에서 가스 누출에 의한 화재사고나 폭발사고가 빈번하게 발생하고 있다. 가스 누출에 의한 사고는 오랜 시간 가스 누출 후 점화원에 의한 폭발이나 화재사고가 대다수를 차지한다. 일반적으로 천연가스를 사용하는 경우 상단부에 가스누출검지기를 설치하고 있다. 하지만 이러한 설치 위치는 대략적인 가스의 특성을 고려한 것이다. 본 연구에서는 가스 누출이 발생할 수 있는 누출위치를 가정의 가스레인지 화구와 밸브 연결부로 선정하고, 전산유체역학(CFD)을 이용하여 가스누출검지기의 시간에 따른 응답특성을 파악하여, 빠른 가스 검지를 위해 가스누출검지기 설치 위치를 최적화하였다. 본 연구의 결과를 기초로 가정이나 건물의 가스누출검지기를 설치할 경우, 가스나 누출되었을 때 신속한 검지를 통하여 위험을 알림으로서 가스폭발이나 사고를 미연에 방지할 수 있을 것이다.

파랑특성을 고려한 조력발전소 여자시스템 적용에 관한 연구

김현한(Kim,Hyun-Han),김광호(Kim,kwang-Ho),정종찬(Jeong,Jong-Chan),박성호(Park,Sung-Ho),옥연호(Ok,Yeon-Ho),최형철(Choi,Hyung-Chol. ) 대한전기학회 2008 대한전기학회 학술대회 논문집 Vol.2008 No.7

관측 강우와 심층신경망의 적용을 통한 도시홍수 분석

김현일(Kim, Hyun Il),이재영(Lee, Jae Young),한건연(Han, Kun Yeun),조재웅(Cho, Jae Woong) 한국방재학회 2020 한국방재학회논문집 Vol.20 No.1

도시유역의 홍수 발생은 주로 저지대의 유수 집중과 배수관거의 만관에 의해 발생하는데, 이는 높은 강우강도와 집중적인양상을 가지는 강우 사상과 함께 나타난다. 도시유역에 대한 유출량 사전 분석을 위해서는 SWMM이 이용될 수 있지만, 대상 유역이 넓고 배수시스템이 복잡한 경우 1차원 도시유출해석에 적지않은 시간이 발생할 수 있다. 또한, 특정 도시 유역에대하여 많지 않은 침수 사례와 침수 유발 관측강우의 부족은 홍수 대비에 대한 기초자료를 제공하는데 어려움이 있다. 홍수를일으킬 위험 강우 발생 시 긴급하게 도시 유출량을 예측하기 위해서, 본 연구에서는 6시간 지속시간을 가지는 전국단위관측 강우사상을 반영하여 심층신경망 모형을 구축하였다. 하나의 강우사상에서 10개의 통계적 특성을 입력 자료로서 고려하였으며, 예측하고자 하는 목표값은 SWMM을 통한 10분 단위 총 누가 월류량으로 하였다. SWMM모의 결과의 적절성을 검증하기위해 2차원 수리해석 모형과 침수흔적도를 연계한 검증을 실시하였다. 대상지역에 대한 SWMM의 모의 시간은 14분이 소요되었으며, 본 연구에서 제시된 심층신경망과 관측 강우 자료 기반 모형은 2~3초 내외에서 수행되었다. 예측은 강남(400) AWS에서2011년 7월 27일에 관측된 강우사상에 대한 총 누가 월류량을 대상으로 하여 모형의 실용성을 검증하였다. Urban flooding primarily occurs due to flash floods in low-lying regions or insufficient capacity of drainage systems. Urban inundation has become more dangerous to residents because of an increase in impermeable areas, uncertainties in urban sewage planning, and the accumulation of sedimentation in drainage pipes. As an urban watershed with a drainage system and an urbanized river is complicated, a specific time interval is required for the preparation and processing of urban runoff analysis. To allow for rapid simulations of urban runoff estimation during heavy rainfall, a deep neural network model that imitates the conditions of a 6-h duration rainfall was developed in this study. Ten different statistical aspects for each rainfall event were considered as input data, and the total accumulated overflow from a manhole was calculated at intervals of 10 min using storm water management model (SWMM). To verify the accuracy of the results from SWMM, the results obtained from a two-dimensional hydraulic model and an inundation trace map were compared. The computational times for the deep neural network and rainfall data-based models proposed in this study were estimated to be within 1 s, whereas the simulation using SWMM required 14 min. The proposed deep learning model was tested using the total accumulated overflow for the rainfall event observed at Gangnam (400) automatic weather station on July 27, 2011. The simulated results agreed with the observed results in terms of the total accumulated discharge.