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김남형,정길생,노환철,백운화,이경광 ( N . H . Kim,K . S . Chung,H . C . Rho,U . H . Pek,K . K . Lee ) 한국축산학회 1986 한국축산학회지 Vol.28 No.8
These experiments were carried out to produce monozygotic twin by bisection of mouse morula. The morula was bisected by microglass needle without any aid of microinstrument or by lateral incision using sharp blade attached to micromanipulator. Bisected dermi-embryos were cultured and transferred to pseudopregnant recipient mouse. The results obtained in these experiments were summarized as follows: 1. Of 285 decompacted morulae bisected by microglass needle, 180 embryos were divided into two demi-embryos, respectively, without any visible damage. 2. Total 180 pairs of demi-embryos separated from decompacted morula and subjected to in vitro culture were resulted in 85(47.2%) pairs of eu-blastocyst, 40(22.2%) pairs of eu-blastocyst and pseudo-blastocyst, 35(19.4%) pairs of pseudo-blastocyst and 20(11.1%) pairs of trophectodermal vesicle and degeneration. 3. Of 255 intact morulae bisected by microblade, 100(39.2%) embryos were divided into two demi-embryos, respectively, without damage. However, 95(37.3%) embryos were bisected into one normal and one damaged demi-embryos, respectively. The percentage of demi-embryos developed to blastocyst after in vitro culture with and without zona pellucida were 71.4 and 64.9% respectively. 4. Total 15 twins were produced following transfer of the 38 pairs of eu-blastocysts developed from demi-embryos to 25 recipients. However, none of pseudo-blastocyst gave birth to young, 5. The percentage of eu-blastocyst cultured with or without zona pellucida and developed to live young following transfer were 11.1 and 9.3% respectively.
김남형,Kim, Nam-Hyeong The Korean Society for Reproductive Medicine 1995 Clinical and Experimental Reproductive Medicine Vol.22 No.3
Microtubules and microfilaments are major cytoskeletal components in mammalian ova that provide the framework for chromosomal movement and cellular division. Extensive changes of cytoskeletal organization occur during maturation and fertilization. The changes in cytoskeletons are essential for the normal meiotic maturation and for the formation of the biparental diploid genome of the embryo, and thus are repeated at each cell cycle during embryonic development. Disturbance of the cytoskeletal organization could result in abnormal gamete development and early embryonic death.
On the Sediment Transport Characteristics of the Bottom Turbulent Boundary Layer
김남형,키요시 타키카와,Kim, Nam Hyeong,Kiyoshi, Takikawa Korean Society of Civil Engeneers 1993 대한토목학회논문집 Vol.13 No.2
본 논문에서는 유한요소법을 2차원 난류경계층에 적용하였으며, 점성유체의 시간의존 비압축성 운동을 시간과 압력장(場)에서 Navier-Stokes방정식과 vorticity방정식을 이용하여 정식 화하였다. 수치계산방법은 Galerkin방법에 기초하였으며, 난류 경계층의 eddy kinematic viscosity에 대해서는 Prandtl의 혼합거리이론을 도입하였다. 난류 경계층에서 파동에 의한 임의 저면에서 저질의 이동을 수치계산하였다. 유한 요소법에 의해 얻어진 결과는 진동흐름에 의한 경계층과 파동에 의한 경계층에서의 특성의 차이를 분명히 하였다. A finite element method(FEM) is presented and applied to the two-dimensional bottom turbulent boundary layer. The time-dependent incompressible motion of a viscous fluid is formulated by using the well-known Navier-Stokes equations and vorticity equation in terms of the velocity and pressure fields. The general numerical formulation is based on Galerkin method and solved by introducing the mixing length theory of Prandtl for eddy kinematic viscosity of a turbulent flow field. Numerical computations of the transport of sediment on an arbitrary sea-bed due to wave motion in the turbulent boundary layer are carried out. The results obtained by the FEM made clear the difference in characteristic features between the boundary layer due to oscillatory flow and the boundary layer due to wave motion.
가상수조에서 자유표면을 가진 유체흐름의 가시화시뮤레이션
김남형(NAM-HYEONG KIM),김남국(NAM-KUK KIM) 한국해양공학회 2000 韓國海洋工學會誌 Vol.14 No.3
SMAC method is, one of the numerical simulation techniques, modified from the original MAC for the time-dependent variation of fluid flows. The Navier-Stokes equation for incompressible time-dependent viscous flow is applied and, Also marker particles which move with the fluid are used. Two-dimensional numerical computations of fluid flow are carried out in a virtual water tank. This paper has shown very well the movements of marker particles using SMAC method.