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구자민,류지성,정규혁,이철우,박응로,박광식 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.4
Endocrine disruption in crucian carp (Carassius auratus) living in the branch of Han River were examined. Vitellogenin level in plasma was measured using ELISA system and aromatase mRNA level in brain was observed using RT-PCR technique. In all female fish, vitellogenin levels were in the range of 20-40㎍/ml and aromatase mRNA expression could be detected on the agarose gel after RT-PCR. However, in case of males, vitellogenin level was elevated in only one fish, while vitellogenin was hardly detected in others. Aromatase was expressed in all males although the levels were relatively lower than the level in female fish. Testis-ova and any other histological changes of reproductive organ were not shown in both sexes.
구자민,황재민,김현희,이원익,손우진 한국임상수의학회 2001 한국임상수의학회지 Vol.18 No.4
A twelve-month old female, shih-tzu, was presented to diagnose a hind limb ataxia to the Animal Teaching Hospital, Gyeongsang National University. Even though a variety of physical and neurological examination was performed, the cause of the ataxia was not understood. In serum analysis, an elevated levels of ALT, $\gamma$-GTP and LDH were found. A gallstone was subsequently observed at the right 9 and 10th intercostal region on ultrasonography and radiography. Cholelithiasis was diagnosed, and then cholecystotomy was undertaken. No complications were occurred after the operation. The gallstone removed by cholecystotomy was composed of calcium, carbonate and oxalate, identified by a black pigment gallstone.
Cell-free Systems: Recent Advances and Future Outlook
구자민,양지호,박현준 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.6
Cell-free systems utilize a subset of cellular components without intact cell wall and/or membranes. The system was first invented for use in fermentation. Subsequent improvements enabled its application in protein synthesis, which is still the most common use of the system. Lately, attempts have been reported where metabolic engineering concepts and techniques were applied to cell-free systems and/or vice-versa. These attempts and advances led to exciting discoveries about biochemical reactions, as well as properties and/or structures of cellular components that make up complex biological systems. This review will first provide a basic overview and brief history of the cell-free system. Then, explanation on recent advances in the field will be provided, followed by notes on the innovative applications. Future outlook of the field will also be covered with the emphasis on how the emerging data science methods can be applied to improve the system and its applicability.
구자민,김대균,류택희,정은환,Yves Lansac,장윤희 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.3
Push-pull-type copolymers − low-band-gap copolymers of electron-rich fused-ring units (such as cyclopentadithiophene; CPDT) and electron-deficient units (such as benzothiadiazole; BT) − are promising donor materials for organic solar cells. Following a design principles proposed in our previous study, we investigate the electronic structure of a series of new CPDTBT derivatives with various electron-withdrawing groups using the time-dependent density functional theory and predict their power conversion efficiency from a newlydeveloped protocol using the Scharber diagram. Significantly improved efficiencies are expected for derivatives with carbonyl [C=O], carbonothioyl [C=S], dicyano [C(CN)2] and dicyanomethylene [C=C(CN)2] groups, but these polymers with no long alkyl side chain attached to them are likely to be insoluble in most organic solvents and inapplicable to low-cost solution processes. We thus devise several approaches to attach alkyl side chains to these polymers while keeping their high efficiencies.
개에서 외부고정법을 이용한 양측성 하악골절의 치유 일례
구자민,김현희,박대식,장흥희,이효종,이후장,송해룡,이용훈,정종태,연성찬 한국임상수의학회 2003 한국임상수의학회지 Vol.20 No.1
A 5-year-old, male mixed breed dog was presented to Gyeongsang National University Animal Hospital for bilateral mandibular open fracture by bite. The dog had loss of the teeth and injury of the oral mucosa. Radiographic and blood examinations were conducted. Fracture lines were observed between first premolar and second premolar, but temporomandibular luxation was not observed on radiographs. In serum analysis, elevated levels of CPK and LDH were found. The dog was anesthetized and prepared for aseptic surgery. Acrylic external skeletal fixator with Kirschiner wire was applied. Pin loosening and malalignment of the right fracture line was observed at 7 weeks after surgery. We applied Kirschner-Ehmer fixator and half pins during the second surgical procedure. At 4 weeks after a second surgical procedure, the fixator and pins were removed from mandible. The fractures were healed with no complications.
Modified Poly(styrene-b-2-vinylpyridine) Anion-selective Membrane for Concentration Cell
구자민,유성일 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
농도차가 있는 용액 사이에 이온선택성 멤브레인을 삽입하면 선택적인 이온 분리에 따른 화학퍼텐셜 차이가 발생하여 외부로 전류를 발생시킨다. 높은 표면적을 갖는 이온선택성 멤브레인을 활용하면 보다 높은 전류 발생을 유도할 수 있기 때문에, 본 연구에서는 나노 기공을 형성할 수 있는 PS-b-P2VP 블록공중합체를 활용하고자 하였다. 이에, nonsolvent induced phase separation을 활용하여 PS-b-P2VP 의 상분리를 유도하여 표면이 P2VP 블록으로 이루어진 연속상의 기공을 형성한 후, P2VP 블록은 quarternization을 시켜 이온 선택성을 부여하였다. 제작된 멤브레인의 표면은 SEM을 활용하여 분석하였고, dye 흡착실험을 통해 이온선택성을 분석하였다. 또한, 개질된 기공형 PS-bP2VP 멤브레인을 농도차전지에 활용하여 다양한 조건에서 전기적 특성을 분석하였다.
구자민,신기삼,김윤배,배종수,허성강,Koo, Ja-Min,Shin, Kee-Sam,Kim, Yoon-Bae,Bae, Jong-Soo,Hur, Sung-Kang 한국재료학회 2005 한국재료학회지 Vol.15 No.12
PAS(Plasma Activated Sintering) process was tried to apply for the fabrication of BMG(Bulk Metallic Glasses) of $Ni_{57}Zr_{20}Ti_{18}Si_5}\;and\;Ni_{57}Zr_{20}Ti_{18}Si_3Sn_2$ from the as-atomized amorphous powder. Compressive strength for the BMG(bulk Metallic Glasses) of $Ni_{57}Zr_{20}Ti_{18}Si_5$ were lower than those of BMG rods produced by warm extrusion ,or copper mold casting method. Microstructural examination by optical microcope, SEM ana EDS showed that oxidation had occurred during PASintering. In order to prevent the powder from the oxidation during PASintering, Ni coating for $Ni_{57}Zr_{20}Ti_{18}Si_5$ amorphous powder by electroless-plating method was performed. Microstructural examination for Ni coated layers after PASintering indicated that the Ni coating had been so effective to prevent powder from oxidation during PASintering. Sintering behaviors of $Ni_{57}Zr_{20}Ti_{18}Si_3Sn_2$ represent the same as those of $Ni_{57}Zr_{20}Ti_{18}Si_5$.
Butorphanol의 투여가 장문합술 후 개의 행동에 미치는 영향
구자민,연성찬,이희천,장홍희,성용증,이효종 한국임상수의학회 2005 한국임상수의학회지 Vol.22 No.1
This study was performed to investigate non-invasive behavioral pain assessment of dogs after surgery, and the analgesic effects of butorphanol after intestinal anastomosis in dogs. In this study, five dogs in the Control Group were anesthetized, but did not undergo surgery. Five dogs in the Analgesic Group were undergone intestinal anastomosis and treated with butorphanol. Five dogs in the Non-analgesic Group were also undergone intestinal anastomosis without analgesic treatment. The dogs in the Analgesic Group received butorphanol (0.4mg/kg, IM) before and immediately after operation, while dogs in Control and Non-analgesic Groups received isovolumetric doses of sterile saline. The behavior of dogs were videotaped for 400mins after anesthesia, during which time a researcher interacted with the dog once per each 80 mins. At each interaction, the researcher recorded behavioral pain score, using University of Melbourne Pain Scale. Interactive and non-interactive behaviors were observed and quantitated by a single observer using focal continuous sampling method. Vocalizations were obtained during 400mins after anesthesia, and duration of call, intensity, pitch, 1-4 Formant were analyzed. Surgery affected an increasing of pain score. During interactions with researcher, greeting behaviors were decreased after surgery. Differences between Analgesic group given analgesic or that given a placebo drug were readily understood using quantitative behavioral measurements and vocalization. Significant difference between Analgesic group given butorphanol or that the given placebo drug was apparent(p<0.05).