http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
낫토균으로 발효한 발효울금의 투여가 마우스의 간 기능 및 혈중 지질 함량에 미치는 영향
강재구(Jae Ku Kang),강효진(Hyo Jin Kang),서지혜(Ji Hye Seo),김선옥(Sun Ok Kim),최정효(Jung Hyo Choi),조도연(Do Yeun Cho),박창교(Chang Gyo Park),이회영(Hoi Young Lee) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.4
본 연구는 낫토균으로 울금을 발효함으로써 울금의 기호성 향상 및 낫토키나아제의 작용으로 울금의 체내이용율을 증가시켜 생리활성 기능이 증가하는지 알아보고자 연구하였다. 울금을 낫토균으로 발효시킨 결과, 칼슘과 아연의 경우 울금에 비하여 발효울금에서 각각 약 6배와 44배 증가를 보였고, 셀레늄의 경우에는 발효울금에서만 새롭게 검출되었다. 항산화 활성 비교실험 결과, 울금과 발효울금의 항산화 활성은 각각의 실험농도에서 비슷한 효과를 나타내었으나 발효울금이 울금보다 항산화 활성이 약간 높았다. 발효울금의 간 기능 개선효과에 미치는 영향을 사염화탄소로 간손상을 일으킨 마우스에서 실험한 결과, AST 및 ALT 효소활성의 유의적인 감소를 보였고(p<0.05) 특히 사염화탄소에 의해 야기된 간조직의 염증, 괴사 및 지방의 축적이 확연히 적어지는 등 정상군에 가깝게 간 손상이 회복되어 발효울금은 간 기능성 개선에서 울금보다 효과적이었다. 낫토균으로 발효한 발효울금의 혈중 지질 개선효과를 평가하기 위해 고지방(20% 지방) 식이로 유도된 마우스에서 발효울금 및 울금의 경구투여에 의한 혈중 지질 함량 변화를 평가한 결과, 고지방 식이에 의해 증가된 총 콜레스테롤과 LDL 농도를 유의적으로 감소시켰으며 HDL 콜레스테롤 농도는 유의하게 증가시켰다(p<0.05). 본 연구에서 울금 및 발효울금 모두 혈중지질을 효과적으로 개선하였으나, 발효울금의 경우 저농도 투여에 의해서도 혈중 지질 개선효과가 나타나 울금에 비해 상대적인 우수성을 나타내었다. 또한 낫토균에 의한 울금의 발효가 울금 특유의 이취에 의한 쓴맛 및 신맛을 유의하게 개선하는 것으로 평가되어 적절한 제품화 과정을 거친다면 기능뿐만 아니라 소비자의 기호를 향상시킬 수 있는 건강 기능성 식품개발에 기여할 것으로 생각된다. The effects of turmeric and fermented turmeric by Bacillus natto on antioxidant activities, liver function recovery of acute hepatotoxicity mice, and serum lipid parameters in high fat diet fed mice were investigated. In the results of antioxidant activity by DPPH method, fermented turmeric had higher antioxidative activity than turmeric. Acute hepatotoxicity was induced by 0.5 ㎖ of carbon tetrachloride (CCl₄) per kg of mice. Unlike turmeric, fermented turmeric significantly reduced the levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) after 5 days compared to the controls with 0.5% methyl cellulose (p<0.05). In addition, higher recovery of liver damage by CCl₄ was observed in mice with fermented turmeric than with turmeric. High fat (20%) diet fed mice were divided into 4 groups to investigate the effects of turmeric and fermented turmeric on serum lipid parameters: C (vehicle), TuL (low dose (80 ㎎/㎏) with turmeric), TuH (high dose (160 ㎎/㎏) with turmeric), FTuL (low dose with fermented turmeric), and FTuH (high dose with fermented turmeric). The levels of LDL-cholesterol and HDL-cholesterol were significantly reduced and increased in FTuL, FTuH and TuH groups compared to the C group, respectively. However, there was no significant change in triglyceride levels by either turmeric or fermented turmeric compared to those by control. Collectively, these results strongly suggest that fermented turmeric by Bacillus natto could be used as a functional food for enhancement of health with better consumer acceptance.
강재구(Jae-Ku Kang),이성학(Sung Hak Lee),김달현(Dal Hyun Kim),이나경(Na-Gyong Lee),박완제(Wan Je Park),이영수(Young Soo Lee) 한국독성학회 1999 Toxicological Research Vol.15 No.2
We have been developing a vaccine (CJ-50002) against Vibrio vulnificus, composed of whole cell lysate of a V. vulnificus O-antigen serotype 4-strain. In order to evaluate the mutagenic potential of CJ-50002, 3 sets of mutagenicity tests were performed. In the reverse mutation test using Salmonella typhimurium TA1535, TA1537, TA98 and TA100, CJ-50002 did not increase the number of revertant at any concentration tested in this study (309.6, 154.8, 77.4, 38.7, 19.4 and 9.7 ㎍/plate). CJ-50002, at concentrations of 309.6, 154.8 and 77.4 ㎍/ml, did not increase the number of cells having structural or numerical chromosome aberration in cytogenic test using Chinese Hamster Lung cells. In mouse micronucleus test, no significant increase in the occurrence of micro nucleated polychromatic erythrocytes was observed in ICR male mice orally administered with CJ-50002 at the doses of 500, 250 and 125 mg/kg. These results indicate that CJ-50002 has no mutagenic potential in these in vitro and in vivo systems.
산양유 및 산양유 발효유가 웅성 설치류의 생식기능과 지구력에 미치는 영향에 관한 연구
임경순,강재구,최기명,배창준,조우제,Im, Kyung-Soon,Kang, Jae-Ku,Choi, Ki-Myung,Pae, Chang-Joon,Joh, Woo-Jea 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.4
Objective: The purpose of this study was to evaluate effects of goat milk and fermented goat milk on reproductive function and stamina of male rodent. Methods: Experiment I: Male ICR mouse was divided into four groups. Group 1 none-treated control; Group 2 received saline; Group 3 received cow milk 10 ml/kg per day for 15 days; Group 4 received goat milk 10 ml/kg per day for 15 days. The cauda epididymal sperm motility and testicular sperm production were investigated. Experiment II: Male SD rat was divided into three groups. Group 1 received saline; Group 2 received goat milk 10 ml/kg per day for 28 days; Group 3 received fermented goat milk 10 ml/kg per day for 28 days. The cauda epididymal sperm motility and testicular sperm production were also investigated. The concentration of testosterone in serum at 1 and 3 weeks after treatment was determined using Immulite 2000 kit. Testes, epididymis, prostate, and seminal vesicle were weighed. Experiment III: Male ICR mouse was divided into four groups. Group 1 none-treated control; Group 2 received saline; Group 3 received goat milk 10 ml/kg per day for 4 weeks; Group 4 received fermented goat milk 10 ml/kg per day for 4 weeks. After treatment, the mouse was forced to swim to test for stamina. Results: In Experiment I, the cauda epididymal sperm motility after in vitro culture for 1 or 3 h was significantly (p<0.05) higher in cow milk and goat milk than in the control and saline. There was no significant difference in the cauda epidymal sperm motility between cow and goat milk. The testicular spermatid number was significantly (p<0.01) higher in goat milk (222.8${\times}10^6$) than in the control (108.6), saline (98.2), and cow milk (118.2). In Experiment II, the cauda epididymal sperm motility after in vitro culture for 1 h was significantly (p<0.05) higher in fermented goat milk than in saline and goat milk. There was no significant difference in the cauda epidymal sperm motility between saline and goat milk but goat milk showed slightly higher sperm motility than saline. After in vitro culture for 3 h, the cauda epididymal sperm motility was significantly (p<0.01) higher in fermented goat milk and goat milk than in saline. The testicular spermatid number was significantly (p<0.05) higher in goat milk than in saline, and significantly (p<0.01) higher in fermented goat milk than in saline. And the serum testosterone levels of rats administered with goat milk or fermented goat milk were increased but were no significant difference among three groups. Also the prostate weight was significantly (p<0.05) increased in the goat and fermented goat milk. In Experiment III, the swimming time in the goat milk and fermented goat milk groups was significantly (p<0.01) longer than in the control and saline. There was no significant difference in the swimming time between goat and fermented goat milk but the fermented goat milk showed slightly longer swimming time than the goat milk. Conclusion: The cauda epididymal sperm motility, the testicular spermatid number and stamina were improved when the mice and rats were drunk with goat milk or fermented goat milk.
백남진,강재구,김달현,목헌,김제학,김현수,Baek, Nam-Jin,Kang, Jae-Ku,Kim, Dal-Hyun,Mok, K.-Hun,Kim, Je-Hak,Kim, Hyun-Su 한국독성학회 1997 Toxicological Research Vol.13 No.3
Antigenic potential of genetically engineered human granulocyte colony-stimulating factor (CJ-50001) was assessed in guinea pigs and mice. In active systemic anaphylaxis (ASA) test, although CJ-50001 at 50 $\mu\textrm{g}$ /head induced anaphylactic responses, CJ-50001 5 $\mu\textrm{g}$ /head alone or 50 $\mu\textrm{g}$ / head with adjuvant did not induce anaphylactic responses. In passive systemic anaphylaxis test (PCA) or passive hemagglutination test (PHA), CJ-50001 did not induce positive responses. It is concluded that, in light of the fact that CJ-50001 was antigenic only in ASA but not in PCA or PHA and also that CJ-50001 is a foreign human recombinant protein to guinea pigs, CJ-50001 may not induce systemic allergic react-ion in its clinical use in human.
합성곱 신경망을 이용한 동결절편의 암세포 전이 여부 자동진단에 관한 예비연구
정대일 ( Dae-il Jung ),강재구 ( Jae-ku Kang ),전혜린 ( Hye-lynn Jeon ),오세종 ( Se-jong Oh ),김성철 ( Sungchul Kim ),김영곤 ( Young-gon Kim ),공경엽 ( Gyungyub Gong ),송인혜 ( In Hye Song ),박소연 ( So Yeon Park ),안수민 ( Soomin 한국정보처리학회 2020 한국정보처리학회 학술대회논문집 Vol.27 No.1
동결절편검사는 수술과 연계하여 암전이 여부를 판단하기 위한 응급한 병리검사가 필요할 때 이용된다. 합성곱 신경망은 이미지 분류에 뛰어난 성능을 보이는 딥러닝 기법으로 본 논문에서는 이를 이용하여 유방암 전이 여부를 자동적으로 진단하는 방법을 제안한다. 실험과정은 전처리, 학습, 후처리의 과정으로 구성되어 있으며, 합성곱 신경망으로는 Resnet-18 모델을 사용하였다. 실험 결과 예측 정확도 및 종양의 최대 길이 정합 여부를 점수로 환산하여 약 0.514 의 결과를 보였다.
김종호(Jong Ho Kim),강재구(Jae-Ku Kang),김달현(Dal Hyun Kim),홍선표(Sun Pyo Hong),김수옥(Soo Ok Kim),이영수(Young Soo Lee) 한국독성학회 1999 Toxicological Research Vol.15 No.2
CJ-50003 is a Japanese encephalitis vaccine which the live attenuated Japanese encephalitis (JE) virus SA14-14-2 has been adapted to monkey kidney Vera cells and inactivated with formalin for use as a human vaccine. In order to evaluate the antigenic potential of CJ-50003, antigenicity tests were performed in guinea pig and mouse-rat model. In active systemic anaphylaxis (ASA) test in guinea pigs, 1- and 10-times of clinical doses (0.05 and 0.5 ㎍/body, respectively) of CJ-50003 was administered subcutaneously for 7 times in 3 weeks. CJ-50003 did not induce any anaphylactic responses in guinea pigs. And in passive cutaneous anaphylaxis (PCA) test using rats with mice anti-serum against CJ-50003, 1- and 10 of clinical doses (0.004 and 0.04 ㎍/body, respectively) of CJ-50003 was administered in mice for 7 times in 3 weeks. The anti-serum against CJ-50003 was administered intradermally at the back of rats, but to the intravenous antigenic challenge positive antigen-antibody reactions were not induced. Therefore, it was considered that CJ-50003 may not induce systemic allergic reaction in its clinical use.
마우스에서 항암제 유발 호중구감소에 대한 CJ-50001 의 회복촉진효과
김제학(Je Hak Kim),김현수(Hyun Su Kim),백남진(Nam Jin Baek),김달현(Dal Hyun Kim),최재묵(Jae Mook Choi),강재구(Jae Ku Kang),김기완(Ki Wan Kim) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.4
Neutropenia is a major dose-limiting side effect of cancer chemotherapy. The therapeutic effects of CJ-50001 were examined on neutropenia caused by anticancer agents. Neutropenia was induced by cyclophosphomide (130 mg/kg), doxorubicin (4.5 mg/kg), and vincristine (1 mg/kg) in normal ICR mice and by cyclophosphamide (200 mg/kg) in CT26 adenocarcinoma bearing BALB/C mice. After the subcutaneous injection of anticancer agents, we administered subcutaneously recombinant human granulocyte-colonystimulating factor (100 ㎍/kg/day) to mice in order to stimulate neutrophil production. In normal and tumor-bearing mice, neutrophil production efficacy of CJ-50001 (rG-CSF) was similar to that of Grasin. These results suggest that CJ-50001 could be effective in its clinical use for neutropenia treatment.