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한국여성에서 자궁내막증의 발생위험도와 Estrogen Receptor-α 유전자 다형성과의 관련성에 관한 연구
이사라,허성은,문혜성,김형래,정혜원 이화여자대학교 의과대학 2003 EMJ (Ewha medical journal) Vol.26 No.2
Objectives: To investigate whether polymorphism of gene encoding estrogen receptor-a is asso-ciated with the risk of endometriosis in Korean women. Material and Methods :We investigated 136 patients with histopathologically confirmed endo-metriosis rAFS stage III/IV and 251 control group women who were surgically proven to have no endometriosis. Polymerase chain reaction(PCR) and restriction fragment length polymorphism (RFLP) of PCR products were done to determine each participant's estrogen receptor-a genotype. Results : The distridution according to PvuII genetic polymorphism of estrogen receptor-a were as follows. PP, Pp and pp were 14.7%(20 women), 39.0%(53 women) and 46.3%(63 women) in the study group and 13.9%(35 women), 38.6%(98 women) and 47.4%(119 women) in the con-trol group, respectively. There was no significant difference between the study group and the control group. Conclusion : The results suggest that estrogen receptor-a genetic polymorphism may not be associated with the development of endometriosis in Korean women. 목적: 자궁내막증은 에스트로겐에 의존적인 질환이므로 에스트로겐의 합성, 대사 및 작용에 관여하는 유전자의 다형성이 자궁내막증의 발생기전에 중요한 역할을 할 수 있다. 이에 본 연구에서는 한국인 여성에서 에스트로겐 수용체-a의 유전자 다형성이 자궁내막증의 발생 위험도를 증가시키는 지에 대해 연구하고자 하였다. 방법: 1996년 9월부터 2003년 8월가지 본원 산부인과에서 수술을 통해 병리조직학적으로 자궁내막증 III기와 IV기를 확인한 한국인 여성 136명을 대상으로 하였다. 대조군은 자궁내막증 환자군과 연령이 비슷한 만삭 산모에서 제왕절개술을 시행하거나 양성 난소낭종으로 수술을 시행 하였을 때, 자궁내막증이 없음을 확인한 여성 251명 으로 하였다. 결과: ER-a 유전자의 PvuII 다형성의 분포는 자궁내막증 환자군에서 PP군이 20명(14.7%), Pp군이 53명(39.0%), pp군이 63명(46.3%) 이었고 대조군에서의 분포는 각각 35명(13.9%), 97명(38.7%), 119명(47.4%)으로 나타났으며 자궁내막증 환자군과 대조군 사이의 유의한 차이는 없었다. Pp, pp형을 가지는 경우가 자궁 내막증 환자의 85.3%(116명), 대조군의 86.1%(216명)로 나타났으며, 이 경우 자궁내막증이 발생할 odds ratio가 0.904(95% CI, 0.519~1.702)로 통계적으로 유의한 차이가 없었다. 결론: 이상의 결과로 볼 때, 한국인 여성에서 자궁내막증의 발생위험과 에스트로겐 수용체 a 유전자 다형성간에 연관성은 없는 것으로 나타났다.
( Eun Byul Lee ),( Jung Eun Choi ),( Jung Hee Kim ),( Wonhee Hur ),( Tian Zhu Li ),( Sung Woo Kim ),( Sung Woo Hong ),( Young Ki Lee ),( Sung Min Kim ),( Joon Ho Lee ),( Seung Kew Yoon ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Background: Hepatitis E virus (HEV) is a hepatotropic virus and has shown the ability of self-assembly through its N-terminally truncated ORF2 (Nt-ORF2). Recently, many researchers have attempted to use the virus like particles (VLPs) as a gene delivery vehicle. In this study, we established the liver specific delivery system using HEV-LP produced in Huh7 cells by recombinant baculovirus expression system. Methods: To produce HEV-LP in mammalian cells, the recombinant baculovirus containing the CMV promoter derived Nt-ORF2 gene was generated in the insect cells, Sf21. Subsequently, the baculovirus was used to infect into the human hepatoma cell line, Huh7 cells. The expression of Nt-ORF2 in Huh7 cells was confirmed by western blot analysis. In addition, the formation of HEV-LP particle was observed by electron microscopy. Next, to determine the liver-specific entrance of HEV-LP, FITC-conjugated HEV-LP infected into the cancer cell lines derived from various organs. After infection, the fluorescence existed in infected cells was detected by confocal microscopy. Results: Nt-ORF2 was highly expressed in Huh7 cells infected with recombinant baculoviruses. Nt-ORF2 expressed in the cells showed the ability of self-assembly and release from the cells. The HEV-LPs produced from Huh7 cells was observed 25 nm in diameter. Furthermore, HEV-LP could enter into the hepatoma cell lines within 4 hr post-infection. Conclusions: These results suggest that HEV-LP was efficiently produced and infected in hepatoma cells and established HEV-LP system could be used as the valuable liver specific transporter to deliver the therapeutic agents.
( Sung Woo Hong ),( Won Hee Hur ),( Yong Ki Lee ),( Jung Eun Choi ),( Sung Woo Kim ),( Tian Zhu Li ),( Jung Hee Kim ),( Sung Min Kim ),( Eun Byul Lee ),( Seung Kew Yoon ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Background: Recent studies have described that cancer stem cell plays a key role radioresistance. The aim of this study is to investigate the molecular and cellular mechanisms of liver cancer stem cell in metastasis after irradiation in HCC. In addition, we found ADAM17 genes associated with liver metastasis that can be used to suppress liver metastasis effectively by molecular regulation. Methods: Both CD133+ and CD133- sorted cells were exposed to γ-irradiation. We investigated the key gene/pathway responsible for metastasis in post-irradiated liver cancer stem cells, CD133+ and CD133- cells using cDNA microarray. Metastatic activities in sorted cells were analyzed by cell migration assay. Also the expressions of the typical genes related metastasis MMP-2 and MMP-9 were also measured by gelatin zymography. Stable cell lines expressing shRNA against human ADAM17 were produced by lentiviral transductions. CD133+ and CD133- cells which were suppressed ADAM17 gene expression were analyzed migration activity. Results: After cDNA microarray analysis, eighty nine metastasis- related genes were up-regulated. Specifically we found that the ADAM17 gene was more increased in CD133+ cells than CD133- cells treated with γ-irradiation. In addition CD133+ cells from radiation exposure were consistently expressed higher levels of vascular endothelial growth factor by ELISA. After irradiation, CD133+ cells migrated more actively, and showed an increased invasion rate compared to CD133- cell. Gelatin zymography showed that MMP-2, -9 proteins expression are significantly higher in CD133+ cells media samples than CD133- cells. Suppression of ADAM17 in CD133+ cells reduced migration activity in treating radiation condition. Conclusions: These results suggest that CD133+ cells have more metastatic capacity than CD133- cells after irradiation. In addition, inhibition of cancer stem cells migration through targeting ADAM17 may be promising the therapeutic against of radiotherapy for HCC.
( Sung Eun Hur ),( Ji Young Lee ),( Hye Sung Moon ),( Hye Won Chung ) 대한산부인과학회 2008 Journal of Womens Medicine Vol.1 No.1
Objective: To investigate the expression of vascular endothelial growth factors (VEGFs) and VEGF receptor 1 and 2 in eutopic endometrium of patients with advanced endometriosis and to understand the role of VEGFs and VEGF receptors in the development of endometriosis. Methods: Endometrial samples were obtained from 65 premenopausal women, 29~44 years of age, undergoing laparoscopic surgery or hysterectomy for non-malignant lesions. Sufficient samples were collected from 33 patients with endometriosis (stages III or IV) and 32 controls without endometriosis, as confirmed by laparoscopic surgery. Expression of VEGFs mRNA and protein and their receptors in the eutopic endometrium were analyzed by RT-QC PCR and Western blotting. The quantitative expression of VEGF-A, -B, and -C in eutopic endometrium from patients with endometriosis was examined throughout the menstrual cycle, and was compared to eutopic endometrial expression in control patients without endometriosis. Results: VEGF-A expression in healthy controls was lower in the secretory phase than in the proliferative phase. In patients with endometriosis, VEGF-A expression was not lower in the secretory phase than in the proliferative phase and was higher than in the secretory phase of healthy controls. The expression of VEGF-B and -C was similar to VEGF-A. The mRNA of VEGF receptors 1 and 2 was expressed at the same level in eutopic endometrium from patients with endometriosis and in healthy controls throughout the menstrual cycle. All eutopic endometrial samples from women with and without endometriosis in both the proliferative and secretory phases of the menstrual cycle showed clear bands at the expected molecular weights for VEGF-A and -B. Conclusions: These results suggest that specific angiogenic factors (VEGF-A, -B, and -C) play important roles in the pathogenesis of endometriosis.
Choi, Eun-Young,Jeon, Min Ku,Lee, Jeong,Kim, Sung-Wook,Lee, Sang Kwon,Lee, Sung-Jai,Heo, Dong Hyun,Kang, Hyun Woo,Jeon, Sang-Chae,Hur, Jin-Mok Elsevier 2017 JOURNAL OF NUCLEAR MATERIALS Vol.485 No.-
<P><B>Abstract</B></P> <P>We present our findings that uranium (U) metal prepared by using the electrolytic reduction process for U oxide (UO<SUB>2</SUB>) in a Li<SUB>2</SUB>O–LiCl salt can be reoxidized into UO<SUB>2</SUB> through the reaction between the U metal and Li<SUB>2</SUB>O in LiCl. Two salt types were used for immersion of the U metal: one was the salt used for electrolytic reduction, and the other was applied to the unused LiCl salts with various concentrations of Li<SUB>2</SUB>O and Li metal. Our results revealed that the degree of reoxidation increases with the increasing Li<SUB>2</SUB>O concentration in LiCl and that the presence of the Li metal in LiCl suppresses the reoxidation of the U metal.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Uranium (U) metal can be reoxidized into UO<SUB>2</SUB> through the reaction between the U metal and Li<SUB>2</SUB>O in LiCl. </LI> <LI> The degree of reoxidation increases with the Li<SUB>2</SUB>O concentration in LiCl. </LI> <LI> The presence of the Li metal in LiCl suppresses the reoxidation of the U metal. </LI> </UL> </P>
한국인 여성에서 중증 자궁내막증의 발생 위험도와 CYP17 유전자 다형성과의 관련성에 관한 연구
허성은 ( Sung Eun Hur ),김미경 ( Mi Kyoung Kim ),이사라 ( Sa Ra Lee ),이지영 ( Ji Young Lee ),문혜성 ( Hye Sung Moon ),정혜원 ( Hye Won Chung ) 대한산부인과학회 2005 Obstetrics & Gynecology Science Vol.48 No.3
목적: 자궁내막증은 가임기 여성의 10-15%를 차지하는 흔한 부인과적 질환이지만 원인과 병태생리는 아직 명확하게 밝혀진 바가 없다. 환경적 요인과 더불어 여러 유전자간의 상호작용에 의하여 생기는 다유전적인 질환으로 알려져 있어 원인 유전자를 찾는 연구들이 활발히 진행 중이다. 이에 본 연구에서는 한국인 여성에서 CYP17의 유전자 다형성이 자궁내막증의 발생 위험도를 증가시키는 지에 대해 알아보고자 하였다. 연구 방법: 연구 대상자는 병리조직학적으로 자 Objective: To investigate whether polymorphism of CYP17 gene is associated with the risk of advanced endometriosis in Korean women. Methods: The 194 endometriosis patients and 209 controls were enrolled. Blood samples were collected from 194 patients with