Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

Effects of dietary supplementation with probiotic CS-A on performance in broiler chickens

Seong Soo Kang1*, Se Eun Kim, Ara Go, Kyung Mi Shim, Chun Sik Bae, Chang Jong Moon, Sung-Ho Kim, Jong-Choon Kim, Jin-Cheol Yoo, Seung Sik Cho 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.3

Probiotics, enzymes, organic acids, oligosaccharides, antioxidants, and other functional materials are actively being explored as alternatives to antibiotics. Probiotics include live beneficial microorganisms that colonize the intestinal tract and competitively inhibit attachment and growth of harmful microbes. Probiotics also increase feed efficiency by assisting in nutrient absorption and digestion. The current study was conducted in order to evaluate the effect of a new probiotic, CS-A, as a dietary supplement of a fermented product on growth performance, feed intake, and feed conversion efficiency in broiler chickens, and to evaluate its value as an alternative for antibiotics used as a feed additive. Antibacterial and anti-inflammatory effects of CS-A were investigated in vitro and the in vivo effects of a constant concentration of supplemented CS-A on growth rate and feed efficiency were evaluated. In addition, the safety of CS-A was assessed by examination of common symptoms and mortality. Determination of minimal inhibitory concentration revealed an excellent antibacterial effect of CS-A. Cytotoxicity was low and anti-inflammatory effects were achieved at the effective concentration of CS-A. Supplementation with 0.1% CS-A resulted in a feed efficiency score of 1.84 in broilers, compared to 2.00 in the control group. There were no adverse clinical findings, necropsy findings, hematology, and altered serum biochemistry parameters, and no mortality. Thus, it is concluded that CS-A is safe and effective as a feed additive.

오뜨 꾸뜨르의 역할에 관한 연구

강경자,김미옥 慶尙大學校 기초과학연구소 1990 基礎科學硏究所報 Vol.6 No.-

The role of Haute-Couture were studied in view point of art and commerce. It has always created a new mode and developed the designing materials and the stable techniques for a work of art. The Haute-Couture has played a pivotal role as a chief source of mode creation and the designers have found their creative ideas on the materials such as a building, art gallery, cinema, costume, and a music. They have displayed their images in clothes on the basis of these materials and their garments were sublimated into art. But the work of art could not made a financial profit on the fantastic and unpractical side, and so, an emphasis on commercialism were to be an essential preposition. For this, Houte-Couture houses were enterprised and merchandized the royalties of ready-made suit, perfumes, accessories, undergarments, jewelry, and handbags to gain profits. The designers have introduced their pieces and creative vogues through the fashion shows. In the end, only when a successive graft between art and commerce is harmonized, there will be a realized Haute-Couture to play the leading role as a creative vogue.

위기상담의 효과적인 개입과 진행과정

강경미 그리스도신학대학교 출판부 2003 교수논문집 Vol.2 No.-

자폐아동에서 FMR-1 유전자에 대한 분자유전학적 연구

강경미,곽동일,이민수 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.6

연구목적: 자폐장애가 유전성이 있다고 제시하는 많은 쌍생아 및 가족연구와 유전자 연구에도 불구하고 이 질환의 유전방식과 관련 유전자는 현재까지 알려져 있지 않고, 이 장애는 임상적으로 이질적 장애이며 유전방식과 관련 유전자는 현재까지 알려져 있지 않고, 이 장애는 임상적 이질적 장애이며 유전적으로도 다인자 질환일 가능성이 높은 것으로 생각된다. 본 연구에서는 자폐장애와 FMR-1 유전자간의 연관성을 조사하고자 자폐장애 아동 총 66명을 대상으로 분자유전학적 검사를 시행하였다. 방 법: 연구대상군은 DSM-Ⅳ 진단기준으로 자폐장애가 해당하고 K-CARS 점수가 30점 이상인 아동들을 대상으로 하였다. Genomic DNA를 전혈에서 추출한후, 탐침 Xho/PstⅠ과 두 개의 제한효소(EcoR Ⅰ, Eag Ⅰ)를 사용하여 썼던 블롯팅을 시행하였다. 결 과: 연구대상 총 66명중 1명의 남아가 메칠화된 모자이크의 양상을 보이었고, 3명의 남아와 2명의 여아는 불완전 변이 양상을 보이었으나 완전 변이의 양상은 없었다. 결 론: 본 연구에서는 FMR-1 유전자가 자폐장애의 유전적 요인과 관련이 있다는 이전의 보고를 뒷받침할만한 명백한 결과를 얻지 못하였다. Objectives: To elucidate an association of the fragile X syndrome with autism, Southern blot analysis was performed in 66 autistic children aged from 2 years to 11 years. Methods: Subjects were 66 autistic children with of autistic disorder diagnosed by DSM-Ⅳ criteria and Childhood Autism Rating Scale-Korean version. Genomic DNA was extracted from peripheral blood and DNA was used to detect a FMR(Fragile Mental Retardation)-1 gene. Xho/PstI probes and two restriction enzymes(EcoRI, EagI)were used for Southern blot analysis. Results: There were one boy with a methylated mosaic pattern and 3 boys and 2 girls with an unmethylated premutation band. But there was no full mutation pattern. Conclusion: Although the possibility of the relationship between autistic disorder and FMR-1 gene has been suggested, the results from this study do not provide any definite association of FMR-1 gene with autism in autistic children.

중환자의 욕창 예방 연구 : 욕창 예방 QI팀을 중심으로

강소영,최은경,김진주,주미정 한국의료QA학회 1997 한국의료질향상학회지 Vol.4 No.1

Background : A pressure sore was defined as any skin lesion caused by unrelieved pressure and resulting in damage to underlying tissue. The health care institutions in the United States were reported the incident rate of pressure sores ranging from 6 to 14%. United states were reported the incident rate of pressure sores in patients rate of pressure sore. Also, Annual expenditures for the care of pressure sores in patients in the United States have been estimated to be $7.5 billion; furthermore, 50 percent more nursing time is required to care for patients with pressure sore in comparison to the time needed to implement preventive measures against pressure sore formation. However, In Korea, there were little reliable reports, or researches, about incidence rates of pressure sore in health care institution including intensive care unit and about the integrated approach like CQI action team for risk assessment, prevention and treatment of pressure ulders. Therefore, this study was to develop pressure sore risk assessment tool and the protocol for prevention of pressure sore formation through CQI action team activities, to monitor incident rate of pressure sore and the length of sore formation for patients at high risk, and to approximately estimate nursing time for sore dressing during research period as the effect of CQI action team. Method : CQI action team in intensive care unit, launched since early 1996, reviewed the literature for the standardized risk assessment tool, developed the pressure sore assessment tool based on the Braden Scale, tested its validity, compared on statistics including incidence rate of pressure sore for patients at high risk. Throughout these activities, CQI action team was developed the protocol.called as St. Marys hospital Intensive Care Unit Pressure Sore Protocol, shifted the emphasis from wound treatment to wound prevention, After applied the protocol to patients at high risk, the incident rate and the period of prevention against pressure development were tested with those for patients who received care before implementation of protocol by Chi-square and Kaplan-Meier Method of Survival Analysis. Result : The CQI action team found that there was significant difference of incidence rate of pressure sores between patients at high risk( control group) who received care implementation of protocol (p<.05). 25% possibility of pressure sore formation was shown for the patients with 6th hospital day in ICU in control group. In experimental group, the patients with 10th hospital day had 10% possibility of pressure sore. Therefore, there was significant difference(p< .05) in survival rate between two groups. Also, nursing time for dressing on pressure sore in experimental group was deceased as much as 50% of it in control group. Conclusion : The collaborative team effort led to reduced incidence, increased the length of prevention against pressure sore, and declined nursing care times for sore dressing. However, there have had several suggestions for future study. The preventive care system for pressure sore should be appleed to patients at moderate, or low risk throughout continuous CQI team activities based on Bed sore Indicator Fact Sheet. Hospital-wide supports, such as incentives, would be offered to participants for keeping strong commitment to CQI team. Also, Quality Information System monitoring incidents and estimation cost of poor quality. like workload(full time equivalence) or financial loss, regularly in a hospital has to be developed first for supporting CQI team activities as well as empowering hospital-wide QI implementation. Being several limitations, this study would be one of the report cards for the CQI team activities in intensive care unit of an acute hospital and a trial of quality improvement of health care in Korea.

GT rotary file을 이용한 근관성형법과 충전방법의 비교

강유미,진정희,유미경,이세준,이광원 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.5

The purpose of this study was to compare the shaping time of two shaping methods and the leakage of three different obturation techniques. Ninty three canaled human molar teeth were used. which were randomly divided into two groups of forty teeth each and ten control teeth. After working length determination. the one group was prepared crowndown technique using rotary root canal instruments of GT rotary files .12/20, .10/20, .08/20 and .06/20 taper(Maillefer Instrument SA. Switzerland). The other group was instrumented with Gates Gildden burs(#1, #2 and #3) to coronal preparation and GT rotary files .08/20 and .06/20 taper to apical preparation. Shaping time was measured. After root canals were instrumented, they were divided to three subgroups and obturated as follows: Subgroup 1, obturated with single cone method : Subgroup 2, obturated with lateral condensation : Subgroup 3, obturated with continuous wave technique. Three subgroups were obturated using non-standardized gutta-percha cone(Diadent, Korea, 06 or .08 taper) and AH-26(Dentsply DeTrey, Germany) as a root canal cement. Ten unobturated teeth served as positive and negative controls. After immersion in 2% methylene blue solution for 1 month, the teeth were washed during 24h. The teeth were determineralized in 10% nitric aciid and dehydrated by immersion in 80, 90 and 100% ethyl alcohol. The teeth were finally cleared and stored in 100% methylsalicylate, and apical dye penetration was evaluated under stereomicroscope(Leica M420, LC, U.S.A) at (×) 8.75 magnification. Liner measurement of dye penetration was assessed with the use of digitalized image analysing system (analySIS, GmbH, Germany), The data were analysed statistically using independent T-test and Two-way ANOVA and Tukey test. The result were as follows: 1. In canal prepared with GT^Trotary file, shaphing time taked more than the group of using Gates Gildden drill to coronal preparation without statistical significant (p>0.05). 2. The group of single cone obturation using canal preparation of GT^TM rotary files showed significantly more apical leakage than those of lateral condensation and continuous wave technique regardless of shaping method (p<0.05). 3. The group of single cone obturation using canal preparation of GT^TM rotary files and Gates Gildden drill showed significantly more apical leakage than those of continuous wave technique regardless of shaping method (p<0.05). 4. Regardless of shaping method. The group of continuous wave obturation showed less apical leakage than those of lateral condensation without statistical significance (p>0.05). 5. The group of single cone obturation using canal preparation of GT^TM rotary files and Gates Gildden drill showed more apical leakage than the group of lateral condensation using sane shaping method without statistical significance (p>0.05).

胃疾患의 食餌療法

강미경,김미숙,김선숙,김수향,양이선 효성여자대학교 가정대학 학도호국단 1987 家政大論集 Vol.6 No.-

The circumstance being surrounded with a rapidly changing society causes us to build up manystresses during our modern life. For this reason, there is increasing the occurrence of gastroentericdisorder in frequency. The dietary treatment of gastroenteric disorder requires to prepare the program necessary fora meal's amount quality and cookery with careful attention. In addition, it is essential for gastroentericpatients to fully supplement the needed nutritive substance depending on the degree of the trouble,by controling their mealtimes. Consequently, the successful dietary treatment entirely depends uponthe close cooperation between the patient involving his family and doctors and dietitians becausethe patient has to observe the standard of the programed dietary treatment. And it also is very impor-tant that the person who prepares table for these patients, attempts to improve their appetite, bysupplying foods suitable for their digestion and taste on the basis of the dietary treatment's standard. Several mental elements of gastroenteric patients have great effect upon the recovery of their di-sorder so that their family and nurses must lead these patients to stabilize their mind and to berestored to health as well as the adequate medical treatment.

뇌경색 환자에 있어 시간경과에 따른 영상변화

강경숙,양동선,김진미 국립서울정신병원 2000 臨床硏究論文集 Vol.2000 No.-

蒼附導痰丸이 白鼠의 排卵에 미치는 影響

강복환,박종문,박경미,조한백,엄주오,유심근 한국전통의학연구소 2003 한국전통의학지 Vol.13 No.1

Changbudodamhwan(CBH) is used in female infertility caused by ovulation disorder. An attempt was made to evaluate the influences of CBH on the serum concentrations of FSH, LH, estradiol(E_(2)) and progesterone, the histological and optical changes of ovary, ovary weight of rats. The results of the study were as follows : 1. Blood FSH level increased experimental group as compared with control group, which showed no efficacy. 2. Blood LH level increased in experimental group as compared with control group, which showed no efficacy. 3. Blood E_(2) level increased in experimental group as compared with control group, which showed no efficacy. 4. Blood progesterone level increased in experimental group as compared with control group, which showed no efficacy. 5. In optically observations of ovary, weight of ovary increased in experimental group as compared with control group, which showed no efficacy. 6. In histological observations of ovary, ovulation increased in experimental group as compared control group, which showed efficacy. According to these results, CBH influence on the ovulation and ovary of rats.