http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Ji Sue Baik ),( Seo Won Mun ),( Kyoung Sook Kim ),( Shin Ji Park ),( Hyun Kyoung Yoon ),( Dong Hyun Kim ),( Min Kyu Park ),( Cheorl Ho Kim ),( Young Choon Lee ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.2
We first demonstrated that cordycepin inhibited cell growth and triggered apoptosis in U87MG cells with wild-type p53, but not in T98G cells with mutant-type p53. Western blot data revealed that the levels of procaspase-8, -3, and Bcl-2 were downregulated in cordycepintreated U87MG cells, whereas the levels of Fas, FasL, Bak, cleaved caspase-3, -8, and cleaved PARP were upregulated, indicating that cordycepin induces apoptosis by activating the death receptor-mediated pathway in U87MG cells. Cordycepin-induced apoptosis could be suppressed by only SB203580, a p38 MAPK-specific inhibitor. These results suggest that cordycepin triggered apoptosis in U87MG cells through p38 MAPK activation and inhibition of the Akt survival pathway.
Baik, Ji-Sue,Kim, Kyoung-Sook,Moon, Hyung-In,An, Hyun-Kyu,Park, Shin-Ji,Kim, Cheorl-Ho,Lee, Young-Choon Oxford University Press 2014 Acta biochimica et biophysica Sinica Vol.46 No.1
<P>In the present study, we firstly found that cordycepin elevated the gene expression of the human GD3 synthase (<I>hST8Sia I</I>) in human neuroblastoma SK-N-BE(2)-C cells. To elucidate the mechanism underlying the upregulation of <I>hST8Sia I</I> gene expression in cordycepin-treated SK-N-BE(2)-C cells, functional characterization of the promoter region of the <I>hST8Sia I</I> gene was performed. Analysis of promoter activity using varying lengths of 5′-flanking region showed a dramatic increase by cordycepin in the −1146 to −646 region, which contains putative binding sites for transcription factors c-Ets-1, CREB, AP-1, and NF-κB. Site-directed mutagenesis for these binding sites and chromatin immunoprecipitation assay revealed that the NF-κB binding site at −731 to −722 is essential for the cordycepin-induced expression of the <I>hST8Sia I</I> in SK-N-BE(2)-C cells. Moreover, the <I>hST8Sia I</I> expression induced by cordycepin was significantly repressed by pyrrolidinedithiocarbamate, an inhibitor of NF-κB. These results suggested that cordycepin induces upregulation of <I>hST8Sia I</I> gene expression through NF-κB activation in SK-N-BE(2)-C cells.</P>
Thapsigargin Induces Platelet Aggregation, thereby Releases Lactate Dehydrogenase from Rat Platelets
Ji Sue Baik,You Na Seo,Man Hee Rhee,Moon-Taek Park,Sung Dae Kim 대한의생명과학회 2021 Biomedical Science Letters Vol.27 No.3
Thapsigargin (TG), a sarco/endoplasmic reticulum (ER) Ca<SUP>2+</SUP>-ATPase (SERCA) inhibitor, has been widely used as an agonist for platelet aggregation for decades. In this study, we investigated the effect of TG on the release of lactate dehydrogenase (LDH) for platelets and elucidated its mechanism. Platelet LDH release and platelet aggregation were increased by TG treatment; 1,000 nM of TG induced the complete lysis of platelets. Other agonists such as collagen (2.5 μg/mL), thrombin (0.1 U/mL), and ADP (10 mM) did not induce significant platelet LDH release despite platelet aggregation. Finally, we investigated the effects of pharmacological inhibitors on TG-induced platelet aggregation and LDH release. SP600125, a JNK inhibitor, and LY294002, a PI-3K inhibitor, inhibited TG-induced platelet LDH release but not platelet aggregation. Forskolin, an adenylyl cyclase activator, also inhibited LDH release without affecting platelet aggregation by TG. These results suggest that the TG-induced platelet aggregation was accompanied by LDH release but regulated by a different signaling pathway.
Baik, Ji Sue,Seo, You Na,Yi, Joo Mi,Rhee, Man Hee,Park, Moon-Taek,Kim, Sung Dae The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.6
This study investigated the inhibitory effect of ginsenoside-Rp1 (G-Rp1) on the ionizing radiation (IR)-induced response in lipopolysaccharide (LPS)-stimulated macrophages and its effects on the malignancy of tumor cells. G-Rp1 inhibited the activation of IR-induced DNA damage-related signaling molecules and thereby interfered with the IR-increased production of nitric oxide (NO) and interleukin (IL)-1β. The inhibitory effect of G-Rp1 increased the survival rate of mice inoculated with CT26 colon cancer cells by suppressing the phenotypic variation of tumor cells induced by conditioned medium obtained from IR- and LPS-treated J774A.1 macrophages.
CHANGING OF RGS TRANSCRIPTS LEVELS BY LOW-DOSE-RATE IONIZING RADIATION IN MOUSE TESTIS
Kim, Tae-Hwan,Baik, Ji Sue,Heo, Kyu,Kim, Joong Sun,Lee, Ki Ja,Rhee, Man Hee,Kim, Sung Dae The Korean Association for Radiation Protection 2015 방사선방어학회지 Vol.40 No.3
Deleterious effects of high dose radiation exposure with high-dose-rate are unarguable, but they are still controversial in low-dose-rate. The regulator of G-protein signaling (RGS) is a negative regulator of G protein-coupled receptor (GPCR) signaling. In addition, it is reported that irradiation stress led to GPCR-mediated mitogen-activated protein kinase (MAPK) and phosphotidylinositol 3-kinase (PI3-k) signaling. The RGS mRNA expression profiles by whole body radiation with low-dose-rate has not yet been explored. In the present study, we, therefore, examined which RGS was modulated by the whole body radiation with low-dose-rate ($3.49mGy{\cdot}h^{-1}$). Among 16 RGS expression tested, RGS6, RGS13 and RGS16 mRNA were down-regulated by low-dose-rate irradiation. This is the first report that whole body radiation with low-dose-rate can modulate the different RGS expression levels. These results are expected to reveal the potential target and/or the biomarker proteins associated with male testis toxicity induced by low-dose-rate irradiation, which might contribute to understanding the mechanism beyond the testis toxicity.
간호학 전공 대학생의 임상 실습 관련 교육 어플리케이션에 대한 사용의도의 영향요인에 대한 연구
조윤정,김영순,박계영,김혜림,최일향,강보영,백진아,정수진,전영지,정연주 이화여자대학교 간호과학대학 2015 이화간호학회지 Vol.- No.49
Purpose: This study examined perceptions and intentions of nursing undergraduates to use educational mobile applications for clinical nursing-practice training. Methods: Participants were 189 junior and senior nursing undergraduates from 14 Korean universities, experienced in clinical practice and educational nursing mobile applications. Using a convenience sampling method, the questionnaire was Sim’s Perceptions and Intentions tool. Statistical analyses were conducted by t-test, one-way ANOVA, correlation, and multiple regression analysis with SPSS 22.0 Windows software. Results: Statistically significant differences emerged between those who used smartphones for more than 4 hours (18.56 ± 4.58) and less than 2 hours (15.84 ± 4.35) per day (F = 3.25, p = .041). Perception and intention of educational-applications use correlated positively (r = .723, p < .001). Performance expectancy (β = .225, p = .002), attitude toward technology (β = .446, p < .001), facilitating condition (β = .132, p = .022), and self-efficacy (β = .168, p = .008) were statistically significant predictors of intention to use educational mobile applications (Adjusted R2 =.616, p < .001). Conclusion: Attitude toward using technology, performance expectancy, and self-efficacy will improve use of nursing educational mobile applications for clinical nursing-practice training. Developers should consider students’ experience in clinical-nursing practice and readiness to use mobile technology.