Lipoxygenase Inhibitors from Paeonia lactiflora Seeds

Hyo-Jin Kim,Shin-Kyo Chung,Sang-Won Choi 한국식품영양과학회 1999 Preventive Nutrition and Food Science Vol.4 No.3

Previously, the methanolic extract of Paeonia lactiflora seeds was shown to have strong inhibitory activity against soybean lipoxygenase (SLO). Four phenolic compounds were isolated from the seeds by solvent fractionation, Sephadex LH-20 column chromatography and preparative HPLC, and three of them showed strong SLO inhibition and were characterized as trans-resveratrol, ε-viniferin and luteolin by UV, IR, ¹H-NMR, ¹³C-NMR and MS spectrometry. trans-Resveratrol (IC_(50)=1.02 μM), ε-viniferin (IC_(50)=O.81 μM) and luteolin (IC_(50)=10.01 μM), first found in the above seeds, exhibited a potent SLO inhibitory activity although their activity was lower than that of a well-known lipoxygenase inhibitor, nordihydroguaiaretic acid (NDGA) (IC_(50)=0.57 μM). These results suggest that Paeonia lactiflora seeds, now an unused plant seed, may be developed into useful sources of anti-inflammatory drugs.

Associations between Maternal sFlt-1/PlGF Ratio and Perinatal and Neonatal Outcomes in Newborns Born to Mothers with Preeclampsia

Hyo Ju Yang,Soo Yeon Lim,Hyun Soo Kim,Chang Won Choi,Younghwa Jung 대한신생아학회 2023 Neonatal medicine Vol.30 No.3

Purpose: The ratio of soluble fms-like tyrosine kinase 1 (sFlt-1) to placental growth factor (PlGF) is considered a predictive marker of preeclampsia. However, the relationship between the sFlt-1/PlGF ratio and perinatal and neonatal outcomes remains unknown. This study aimed to determine the associations of the sFlt-1/PlGF ratio with perinatal and neonatal outcomes in newborns born to mothers with preeclampsia. Methods: This retrospective cohort study reviewed singleton neonates born to mothers with preeclampsia who underwent testing for the sFlt-1/PlGF ratio. We investigated the relationship between maternal sFlt-1/PlGF ratios and gestational age (GA), birth weight (Bwt), Bwt z-score, morbidities, and mortality of neonates born to mothers tested for the sFlt-1/PlGF ratio. Maternal sFlt-1/PlGF ratios examined within 30 days before delivery were used for analysis. Neonatal morbidities and mortality were investigated only in preterm infants born earlier than 32 weeks GA. Results: A total of 225 neonates were included, of which 163 (72.4%) were preterm infants. GA (R=– 0.577, P<0.001), Bwt (R=–0.713, P<0.001), and Bwt z-score (R=–0.608, P<0.001) exhibited significant negative correlations with the sFlt-1/PlGF ratios. Among the 50 preterm infants born earlier than 32 weeks GA, neonatal morbidities were not significantly associated with the sFlt-1/PlGF ratio after adjusting for GA and Bwt. Conclusion: In mothers with preeclampsia, a higher sFlt-1/PlGF ratio was associated with the delivery of newborns with lower GA and lower Bwt. However, this ratio was not associated with increased morbidity or mortality in premature infants born earlier than 32 weeks GA. Purpose: The ratio of soluble fms-like tyrosine kinase 1 (sFlt-1) to placental growth factor (PlGF) is considered a predictive marker of preeclampsia. However, the relationship between the sFlt-1/PlGF ratio and perinatal and neonatal outcomes remains unknown. This study aimed to determine the associations of the sFlt-1/PlGF ratio with perinatal and neonatal outcomes in newborns born to mothers with preeclampsia. Methods: This retrospective cohort study reviewed singleton neonates born to mothers with preeclampsia who underwent testing for the sFlt-1/PlGF ratio. We investigated the relationship between maternal sFlt-1/PlGF ratios and gestational age (GA), birth weight (Bwt), Bwt z-score, morbidities, and mortality of neonates born to mothers tested for the sFlt-1/PlGF ratio. Maternal sFlt-1/PlGF ratios examined within 30 days before delivery were used for analysis. Neonatal morbidities and mortality were investigated only in preterm infants born earlier than 32 weeks GA. Results: A total of 225 neonates were included, of which 163 (72.4%) were preterm infants. GA (R=– 0.577, P<0.001), Bwt (R=–0.713, P<0.001), and Bwt z-score (R=–0.608, P<0.001) exhibited significant negative correlations with the sFlt-1/PlGF ratios. Among the 50 preterm infants born earlier than 32 weeks GA, neonatal morbidities were not significantly associated with the sFlt-1/PlGF ratio after adjusting for GA and Bwt. Conclusion: In mothers with preeclampsia, a higher sFlt-1/PlGF ratio was associated with the delivery of newborns with lower GA and lower Bwt. However, this ratio was not associated with incr

Characterization of KRC-108 as a TrkA Kinase Inhibitor with Anti-Tumor Effects

( Hyo Jeong Lee ),( Yeongyu Moon ),( Jungil Choi ),( Jeong Doo Heo ),( Sekwang Kim ),( Hari Krishna Nallapaneni ),( Young-won Chin ),( Jongkook Lee ),( Sun-young Han ) 한국응용약물학회 2022 Biomolecules & Therapeutics(구 응용약물학회지) Vol.30 No.4

Tropomyosin receptor kinase A (TrkA) protein is a receptor tyrosine kinase encoded by the NTRK1 gene. TrkA signaling mediates the proliferation, differentiation, and survival of neurons and other cells following stimulation by its ligand, the nerve growth factor. Chromosomal rearrangements of the NTRK1 gene result in the generation of TrkA fusion protein, which is known to cause deregulation of TrkA signaling. Targeting TrkA activity represents a promising strategy for the treatment of cancers that harbor the TrkA fusion protein. In this study, we evaluated the TrkA-inhibitory activity of the benzoxazole compound KRC-108. KRC-108 inhibited TrkA activity in an in vitro kinase assay, and suppressed the growth of KM12C colon cancer cells harboring an NTRK1 gene fusion. KRC-108 treatment induced cell cycle arrest, apoptotic cell death, and autophagy. KRC-108 suppressed the phosphorylation of downstream signaling molecules of TrkA, including Akt, phospholipase Cγ, and ERK1/2. Furthermore, KRC-108 exhibited antitumor activity in vivo in a KM12C cell xenograft model. These results indicate that KRC-108 may be a promising therapeutic agent for Trk fusion-positive cancers.

Study on thermal conductivity and electrical resistivity of Al-Cu alloys obtained by Boltzmann transport equation and first-principles simulation: Semi-empirical approach

Choi, Garam,Kim, Hyo Seok,Lee, Kyungmoon,Park, Sang Hyun,Cha, JinHyeok,Chung, In,Lee, Won Bo ELSEVIER SCIENCE 2017 JOURNAL OF ALLOYS AND COMPOUNDS Vol.727 No.-

<P><B>Abstract</B></P> <P>Boltzmann transport equation (BTE) and first-principle approaches are applied in the estimation of the thermal conductivity and electrical resistivity of Al-Cu alloys with varying Al-Cu ratios. The electronic transport properties of various Al-Cu alloys are predicted by solving the BTE using a relaxation time approximation (RTA). The theoretical results are estimated through fitting of experimental data. It is shown that the results correctly estimate transport property trends in terms of temperature and composition. The transport properties are also estimated using a semi-empirical method based on experimental electrical resistivity data. It is believed that the primary reason for deviations between the experimental and theoretical values is computational limits arising from a lack of rigor in taking scattering effects and structural stability into account. This explanation for the deviation is also supported by the WF law and relaxation times (or scattering rate).</P> <P><B>Highlights</B></P> <P> <UL> <LI> BTE and first-principles reliably estimate the κ and ρ of Al-Cu alloys. </LI> <LI> The studied semi-empirical method is effective to approximate the relaxation times. </LI> <LI> There are a few deviations between the experimental and theoretical values. </LI> <LI> It is computational limits by scattering effects and structural stability. </LI> <LI> The study can be applied to estimate the κ and ρ of Al-Cu alloys or other alloys. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Inhospital Spontaneous Acute Subdural Hematoma (SADH) Patient with Antiplatelet Therapy due to Acute Cerebral Ischemia - A Case Report -

Hyo Chang Kim,Chun-Sik Choi,Jae-Young Yang,Hyun-Chul Shin,Yu-Sam Won,Young-Jun Kwon 대한중환자의학회 2011 Acute and Critical Care Vol.26 No.2

A case of “spontaneous” acute subdural hematoma caused by aspirin and plavix therapy has not been described previously. As an isolated cerebrovascular event related to aspirin and plavix therapy, this is the first report described in the literature. It also represents a new differential diagnosis for nontraumatic acute subdural hematomas.

Modification of ginsenoside saponin composition via the CRISPR/Cas9-mediated knockout of protopanaxadiol 6-hydroxylase gene in Panax ginseng

Choi Han Suk,Koo Hyo Bin,Jeon Sung Won,Han Jung Yeon,Kim Joung Sug,Jun Kyong Mi,최용의 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.4

Background: The roots of Panax ginseng contain two types of tetracyclic triterpenoid saponins, namely, protopanaxadiol (PPD)-type saponins and protopanaxatiol (PPT)-type saponins. In P. ginseng, the protopanaxadiol 6-hydroxylase (PPT synthase) enzyme catalyses protopanaxatriol (PPT) production from protopanaxadiol (PPD). In this study, we constructed homozygous mutant lines of ginseng by CRISPR/ Cas9-mediated mutagenesis of the PPT synthase gene and obtained the mutant ginseng root lines having complete depletion of the PPT-type ginsenosides. Methods: Two sgRNAs (single guide RNAs) were designed for target mutations in the exon sequences of the two PPT synthase genes (both PPTa and PPTg sequences) with the CRISPR/Cas9 system. Transgenic ginseng roots were generated through Agrobacterium-mediated transformation. The mutant lines were screened by ginsenoside analysis and DNA sequencing. Result: Ginsenoside analysis revealed the complete depletion of PPT-type ginsenosides in three putative mutant lines (Cr4, Cr7, and Cr14). The reduction of PPT-type ginsenosides in mutant lines led to increased accumulation of PPD-type ginsenosides. The gene editing in the selected mutant lines was confirmed by targeted deep sequencing. Conclusion: We have established the genome editing protocol by CRISPR/Cas9 system in P. ginseng and demonstrated the mutated roots producing only PPD-type ginsenosides by depleting PPT-type ginsenosides. Because the pharmacological activity of PPD-group ginsenosides is significantly different from that of PPT-group ginsenosides, the new type of ginseng mutant producing only PPD-group ginsenosides may have new pharmacological characteristics compared to wild-type ginseng. This is the first report to generate target-induced mutations for the modification of saponin biosynthesis in Panax species using CRISPReCas9 system.

동구릉 일원의 입지와 공간구성 특성에 관한 연구

( Won Ho Lee ),( Hyo Jeong Kim ),( Sang Hee Moon ),( Jong Hee Choi ) 한국전통조경학회(구 한국정원학회) 2007 Journal of Korean Institute of Traditional Landsca Vol.5 No.-

Case Report : Pure red-cell aplasia and autoimmune hemolytic anemia in a patient with acute hepatitis A

( Hyo Jeong Chang ),( Dong Hyun Sinn ),( Sung Gyun Cho ),( Tae Hoon Oh ),( Tae Joo Jeon ),( Won Chang Shin ),( Won Choong Choi ) 대한간학회 2014 Clinical and Molecular Hepatology(대한간학회지) Vol.20 No.2

Pure red cell aplasia (PRCA) and autoimmune hemolytic anemia (AIHA) have rarely been reported as an extrahepatic manifestation of acute hepatitis A (AHA). We report herein a case of AHA complicated by both PRCA and AIHA. A 49-yearold female with a diagnosis of AHA presented with severe anemia (hemoglobin level, 6.9 g/dL) during her clinical course. A diagnostic workup revealed AIHA and PRCA as the cause of the anemia. The patient was treated with an initial transfusion and corticosteroid therapy. Her anemia and liver function test were completely recovered by 9 months after the initial presentation. We review the clinical features and therapeutic strategies for this rare case of extrahepatic manifestation of AHA. (Clin Mol Hepatol 2014;20:204-207)

First-principles study of electronic transport coefficients of point-defective metallic species: aluminum and its bimetallic alloys

Choi, Garam,Kim, Hyo Seok,Park, Sang Hyun,Cha, JinHyeok,Chung, In,Lee, Won Bo IOP 2019 Modelling and simulation in materials science and Vol.27 No.3

<P>The electronic transport coefficients, such as electrical and thermal conductivity and the Seebeck coefficient, of aluminum (Al) and its bimetallic alloys (Al<SUB>2</SUB>Cu, Al<SUB>12</SUB>Mg<SUB>17</SUB>, AlZr<SUB>3</SUB>) with point defects are studied based on first-principles calculations and the Boltzmann transport equation. Defects in alloys can lead to changes in electronic structures, such as group velocity and density of states, resulting in deviations in transportation distributions. In this manner, these defects affect electronic transport coefficients. In this study, changes of transport coefficients by defects are described by temperature and chemical potential energy. The defects have a negative impact on the conductivity of metal alloys. However, it is found that this effect is relatively small at certain Wyckoff sites. In addition, it is noted that the defects may lead to useful features in terms of thermoelectricity. This study will support understanding and designing the metal alloys by theoretical anticipations of the transport coefficients by defects, according to changes of temperature and chemical potential energy.</P>

Novel Index of Coronary Collateral Development as a Useful Predictor of Clinical Outcome in Type 2 Diabetic Patients With Coronary Artery Disease

Choi, Eue-Keun,Kim, Hyo-Soo,Park, Kyung-Woo,Kim, Hyung-Kwan,Cho, Joung-Won,Lee, Myoung-Mook,Park, Young-Bae,Choi, Yun-Shik Japanese Circulation Society. 2005 CIRCULATION JOURNAL Vol.69 No.7

<P><B>Background</B> There is a paucity of information regarding the impact of the coronary collaterals on prognosis in type 2 diabetic (T2DM) patients. We developed a novel index, which considers not only the degree of collateral circulation but also the stimulus of collateral development, and investigated its prognostic value in T2DM patients with coronary artery disease (CAD). <B>Methods and Results</B> One hundred and ninety four consecutive T2DM patients were analyzed and followed for an average of 30 months. We measured the diameter stenosis (DS; %), corrected TIMI frame count (CTFC) and Rentrop score at 3 major epicardial coronary arteries. The collateral development (CD) score was calculated by: (Σ Rentrop score +1)/Σ [DS (%) × CTFC] ×1,000. During the follow-up, acute cardiovascular events occurred in 49 patients. By multivariate analysis, the CD score was an independent predictor of adverse events not only in the total population (p<0.001), but in all 3 subgroups (p=0.020 for coronary artery bypass grafting, p=0.030 for percutaneous transluminal coronary angioplasty (PTCA) and p=0.003 for the medical group). Furthermore, patients in the tertile with the highest CD score showed improved survival by Kaplan-Meier analysis in the total population, the PTCA and the medical group. <B>Conclusions</B> The CD score, a novel index of collateral development, may be a useful predictor of clinical outcome in T2DM patients with CAD. (<I>Circ J</I> 2005; <B>69:</B> 786 - 792)</P>