느타리버섯 세균성갈빈병균 Pseudomonas tolaasii의 효소면역검출법

이향범,전낙범,손동화,유승헌 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

느타리버섯의 세균성갈반병균인 P. talaasii(PT)를 신속 간편하게 검출할 수 있는 효소면역측정법(ELISA)을 개발하였다. 특이항체를 생산하기 위하여 PT(5×10 exp (7) cfu)를 Freund's adjuvant와 함께 토끼에 수차례 면역하였으며, 가장 높은 역가를 나타낸 항혈청을 이용하여 비경합 ELISA 및 경합 ELISA를 각각 확립하였다. 표준곡선으로부터 PT의 그 검출한계는 각각 2×10 exp (2) cfu/ml 및 3×10 exp (2) cfu/ml가량으로 나타났다. 교차반응은 비경합 ELISA의 경우 P. agarici, P. reactans 및 비병원성 Pseudomonas속 균주와 1/10^3이하의 매우 미약한 반응을 보였으나 P. salanacearum, Erwinia chrysanthemi, Streptococcus mutans, Xanthomonas citri 및 곰팡이인 Fusarium oxysporum 등 다른 균주와의 반응성이 거의 없었으며, 경합 ELISA의 경우 PT이외에는 같은 속 균주에 대하여도 반응성이 거의 없었다. ELISA를 이용하여 버섯으로부터 분리된 18균주에 대하여 PT의 여부를 조사하였을 때 병원성과 white line 반응성을 동시에 나타내었던 11균주에 대하여만 명확한 양성으로 나타나, 본 연구에서 확립한 ELISA는 간편하고 정확한 PT검출법임이 확인되었다. For simple and rapid detection of Pseudomonas tolaasii (PT), a bacterial brown blotch pathogen of oyster mushroom, enzyme-linked immunosorbent assays (ELISA) were developed. To produce specific antibody, PT (5 × 10 exp (7) cfu) and Freund's adjuvant were subcutaneously immunized into rabbits several times. By using the antiserum showing the highest titer, we established noncompetitive and competitive ELISA's. Standard curves of the ELISA's showed that the detection limits were 2 × 10 exp (2) cfu/ml and 3 × 10 exp (2) cfu/ml, respectively. When investigated by noncompetitive ELISA, cross reactivities of the anti-PT antibodies against P. agarici, P. reactans, and other fluorescent Pseudomonas spp. were very low (<1/10^3), but those against P. solanacearum, Erwinia chrysanthemi, Streptococcus mutans, Xanthomonas citri, and a fungus Fusarium oxysporum were almost none. However, when investigated by competitive ELISA, the reactivities against any other strains except PT were almost none. When the ELISA's were applied to 18 strains derived from mushrooms in order to identify PT, only 11 strains showing both pathogenicity and white line reactivity were obviously positive. These results showed that the ELISA's could be convenient tools to detect PT in accordance with existing methods.

Retinoid 투여로 인한 四肢畸形 발생과정에 대한 조직화학적 연구

李鄕愛,金恩英,朴善華,全容赫 고려대학교 의과대학 1993 고려대 의대 잡지 Vol.30 No.1

Histochemistry of carbohydrate residues and morphohistological changes and/with their correlation during the process of the drug-induced limb dysmorphogenesis, following single intragastric dose of etretinate (100mg/kg, body wt.) in DDY mice on day either 10 or 11 of gestation, were analysed. Deformities were largely stage-depedent as, exposure to etretinate on gestational day (GD) 11 with paddle-shaped buds resulted in meromelic defects in 100%, whereas on GD 10 with semilunar buds, slightly retarded morphogenesis ; the drug distrubed mainly on core mesenchymal condensation-formation of precartilaginous zone, thus it appears that the most susceptible period to the etretinate in the limb development is the stage of mesenchymal condensation and the drug-induced cell death in the core mesenchyme was associated with the zones of programmed or phygiological cell death (PCD) but expanded even to the apical ectodermal ridge (AER) and the phenomena lasted longer. PAS·PAPS reactivities and lectin binding of glycoconjugates and CHO residues were differently expressed depend on tissue/stage-specific ; PAS·PAPS positive materials and bindings of Con A, RCA, WGA were gradually increased in epithelia and AER with the development proceed and in chondrogenesis, PAS·PAPS reactivity persisted strongly, while Con A, WGA and RCA expression were temporarily rised in initiation of condensation but decreased thereafter ; etretinate increased Con A bindings in epithelia but reduced expressions of other lectins and glycoconjugates especially in chondrogenesis. Although the drug affected the expression of lectin bindings and glycoconjugates, it seems that these results are caused by in consequence of the morphohistological changes. The results suggest that inhibition of chondrogenesis with subsequent malformation has a primary association with the excessive cell death in the regions of PCD but initial role that caused PCD to expand, accelerate and last longer could be consequence of cytotoxicity, inhibited extracellular matrix production, a combination of these effects or some other effect such as change of gene expression which remains a question.

단독주택의 외관형태와 이미지평가에 관한 연구

이청웅,박화수,박길우,이향미 朝鮮大學校 建設技術硏究所 2002 建設技術硏究 Vol.22 No.1

This study analyzes the types of external forms of detached housing buildings, and conducts the S.D test with it and is to structurize the image of external form of buildings. Types of 24 detached housing buildings are analyzed in this vein and the results are as follows: (1) Mass type in the primary cognitive factor of building is mostly simple or multiple box type. (2) As a result of analyzing the secondary cognitive factor of building, horizontal type is most and it shows that it is the favorable structure in the stability of form. Roof shape and side pattern are mostly gable and plat roofs and it is known that traditional gable roof and flat roof influenced by modern construction are shown in housing. (3) To examine the tertiary cognitive factor, surface shape is mostly flat type and it shows a little monotonous composition. Also finishing materials are concrete and brick. S.D test of detached houses doesn't show the extreme psychological test disposition and does somewhat affirmative evaluation. As a result of conducting the factor analysis based on S.D test, nine psychological factors including 「originality」, 「pleasantness」, 「harmony」, 「ornamentation」, 「openness」, 「balance」, 「rhythm」, 「stability」 and 「orientation」. These can be called psychological factor axis which can evaluate the modern detached house.

T-2 Toxin이 병아리 비장세포의 유전질 발생에 미치는 영향

전향숙,정덕화,이서래 이화여자대학교 생명과학연구소 1994 생명과학연구논문집 Vol.5 No.-

시험관 내에서 T-2 toxin이 병아리 비장세포의 blastogenesis에 미치는 영향을 살펴본 결과, B-cell mitogen인 lipopolysaccharide및 T-cell mitogen인 concanavalin A자극에 대해 T-2 toxin의 농도가 증가함에 따라 억제정도가 증가하는 경향을 나타내었다. 노출시기를 달리하여 T-2 toxin을 투여한 병아리 비장세포에서 mitogen 자극에 대한 반응을 알아 본 결과, 부화하기 전과 후에 계속 T-2 toxin에 노출시킨 실험군은 가장 영향을 많이 받은 것으로 나타났고 부화전 혹은 부화후 어느 한 시기에만 T-2 toxin에 노출된 실험군은 비교적 영향을 적게 받는 것으로 나타났다. The effects of T-2 toxin of mitogen-induced blastogenesis of chick splenic cells were investigated. The[^3H]thymidine incorporation in splenic cells stimulated by lipopolysaccharide and concanavalin A were equally inhibited as the concentration of T-2 toxin was increased. The effective dose of T-2 toxin causing a 50% reduction of[^3H]thymidine incorporation was inbetween 1.0 and 5.0 ng/㎖ for both mitogens. Mitogen-induced blastogenesis in chick splenic cells showed differences among experimental groups with different exposure time of T-2 toxin, exhibiting the most inhibition in the experimental group exposed to T-2 toxin at both embryonic and chick periods.

여성 간질 환자에서 항경련제 복용에 따른 대사 및 생리주기의 변화

김지영,이진화,김숙희,이향운 이화여자대학교 의과대학 2005 EMJ (Ewha medical journal) Vol.28 No.2

Background & Objectives : Women with epilepsy(WWE) are at risk for reproductive and metabolic disorders. This study was performed to investifate whether WWE are more likely to have menstual or metabolic abnormalities, and whether some antiepileptic drugs(AEDs) more likely provoke those problems. Methods : WWW aged 15-50 years old, taking one or more AEDs for at least 6 months, were recruited. Subjucts checked their oral temperature each morning. Body mass index(BMI : kg/㎡) and waist to hip (W/H) ratio were calculated as obesity markers. Hirsutism index, as a marker of polycystic ovary syndrome, was calculated by Ferriman-Gallwey score(hirsutism if score>8). Serum tests ofr gonadotrophins, steroid hormones, sex hormone binding globulins (SHBG), lipid profiles, insulin were performed on menstrual cycle days 3 to 5. Results : Among 54 patients, 18 women were diagnosed as primary generalized epilepsy(PGE) and the other 36 were localoization-related epilepsy(LRE). Also, 21 women(38.9%) were treated with carbamazepine(CBZ), 14 women(25.9%) with valproate(VPA), and 19(35.2%) with lamotrigine(LTG) or topiramate(TPM). Menstrual disturbance was found in 60.0% of PGE versus 30.6% of LRE patients(p=0.050), while 64.3% of VPA and 28.6% of CBZ-treated patients(p=0.036). The W/H ratio and BMI were higher in VPA, than in CBZ-treated patients(p=0.048 and 0.018). The total and HDL-cholesterols decreased in the VPA group compared to the CBZ group(p=0.025 and 0.009). Conclusion : WWE are more likely to experience menstrual and metabolic alterations. WWE with VPA tend to have abdominal obesity and alterations in lipid metabolism.

순천대학교 사범대학 발전 및 특성화 방안 연구

신란식,신향근,이화자 순천대학교 1996 大學發展硏究論文集 Vol.2 No.-

The purpose of this study was to present development plans and to draw up measures to specialize the College of Education (COE) as a teacher training institute after analyzing the problems with which COE in confronted. The plans and measures are primarily to aim attracting many students with better qualities and to assist them to have teaching jobs under the current system of Examination for Teacher Appointment. This study involved multiple data sources obtained from literature review questinnaire survey, and personal contact with the faculty of COE, Questions in the questionnaire were addressed to ask the faculty´s opinions on a curriculum, a school system in university, a practicum for teacher training, a middle and a high school attached to COE, establishing an in-service teacher training institute in COE, and other measures related to the specialization of COE. The findings of the study indicated that the following suggestions should be realized if COE is to be developed and specialized ; 1. A special selection procedure should be applied to attract students with high qualities, 2. Education for the teaching of subject matters should be reinforced and a new curriculum be developed to meet the needs of COE, 3. Appropriate measures should be devised to carry out substantial teacher training for student teachers, 4. A curriculum committe should be formed and asked to work for the management of the curriculum suitable for the specialization of COE, 5. A multi-major or a minor system should be introduced across the student´s specialization fields, 6. A department needed in COE can be established as a major area under the school system in university, 7. The establishment of a middle and a high school attached to COE should be proposed to the Ministry of Education, 8. An educational research center should be established to investigate into issue related to pro-service and in-service education continually, 9. An institute for in-service teacher training and a community education center should be established to render services to the local community, and 10. A second building of COE should be constructed to be equipped with sufficient educational facilities.

Artificial Dermis Composed of Gelatin, Hyaluronic Acid and (1\longrightarrow3),(1\longrightarrow6)-$\beta$-Glucan

Lee, Sang-Bong,Jeon, Hyun-Wook,Lee, Young-Woo,Cho, Seong-Kwan,Lee, Young-Woo,Song, Kang-Won,Park, Moon-Hyang,Hong, Sung-Hwa The Polymer Society of Korea 2003 Macromolecular Research Vol.11 No.5

Porous scaffolds composed of gelatin and polysaccharides such as hyaluronic acid and $\beta$-glucan were prepared by using the freeze-drying method after cross-linking with l-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). The scaffold had an inter-connected pore structure with the sufficient pore size for use as a support for the growth of fibroblasts. Results for the contact angle and cell attachment confirmed that high gelatin content in a mixture was suitable for cellular attachment and distribution in two- or three-dimensional fibroblast cultures. However, the addition of polysaccharides aroused the synergistic effects of morphologic and mechanical property of gelatin-based scaffolds. To prepare the artificial dermis for the wound dressing to mimic the normal human dermal skin, fibroblasts were isolated from a child's foreskin, and cultured in gelatin-based scaffolds. An in vivo study showed that the artificial dermis containing the fibroblasts enhanced the wound healing rate and re-epithelialization of a full-thickness skin defect rather than the acellular scaffold after one week.

Ultrasound-guided restaging and localization of axillary lymph nodes after neoadjuvant chemotherapy for guidance of axillary surgery in breast cancer patients: Experience with activated charcoal.

Lee, Soo Jung,Lee, In Hee,Chae, Yee Soo,Lee, Jeeyeon,Jung, Jin Hyang,Park, Ho Yong,Kim, Won Hwa,Kim, Hye Jung,Kang, Byung Woog Grune & Stratton 2018 Journal of clinical oncology Vol.36 No._suppl15

Development of a Competitive Direct Enzyme-Linked Immunosorbent Assay for Teicoplanin

( Hyang Burm Lee ),( Bo Yeon Kwak ),( Jae Chan Lee ),( Chang Jin Kim ),( Dong Hwa Shon ) 한국미생물생명공학회 2004 Journal of microbiology and biotechnology Vol.14 No.3

Assessment of General and Cardiac Toxicities of Astemizole in Male Cynomolgus Monkeys: Serum Biochemistry and Action Potential Duration

Lee, Jong-Hwa,Kim, Do-Geun,Seo, Joung-Wook,Lee, Hyang-Ae,Oh, Jeong-Hwa,Shin, Ho-Chul,Yoon, Seok-Joo,Kim, Choong-Yong Korean Society of ToxicologyKorea Environmental Mu 2008 Toxicological Research Vol.25 No.2

Toxicology screening following treatment with astemizole, a histamine receptor antagonist, at oral doses of 0, 10, 30 and 60 mg/kg was carried out in male cynomolgus monkeys (Macaca fascicularis). No dose-related changes in mortality, clinical signs, body weight changes, food consumption, or urine analysis occurred in any animal compared to the vehicle control. However, the high-dose group showed a decrease in BUN and ALP compared to vehicle control group. In addition, the levels of TG, AST, ALP and CK increased. Although astemizole did not produce significant toxicological changes at any dose tested, we predict that it can cause toxicological changes of the liver and heart based on the changes in the serum parameters related to the heart and liver. The Action Potential Duration (APD) was prolonged in the heart of 60 mg/kg treatment group compared to the control group. The APD increase in 60 mg/kg treatment group along the other related changes in toxicological parameters imply that astemizole has major cardiotoxic effects in the cynomolgus monkey. This study is a valuable assessment for predicting the general toxicity and cardiotoxic effects of antihistamine drugs using nonhuman primates.