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보리새우, Penaeus japonicus의 中腸에서의 Glycine Transport
朱鎭球,宋容圭,劉寬凞,朴來鉉,申埈國 충남대학교 자연과학연구소 1983 忠南科學硏究誌 Vol.10 No.2
Glycine transport through mucosal border of the columnar epithelium on the marin shrimp, P. japonicus midgut has been investigated with particular emphasis on the interaction to Na^+. The transmural fluxes of the amino acid across the midgut wall were also determined. 1. Mucosal glycine uptake occurred via Na^+-dependent and Na^+-independent carrier processes, and the passive movement of glycine. 2. Mucosal and serosal glycine uptakes in normal saline were higher than those in Na^+-free medium, but lower than those in Cl^--free medium. 3. L-alanine was acted like a competitive inhibitor of mucosal glycine entry process. 4. Exit process of glycine across the mucosal membrane was accelerated by the presence of L-alanine in the medium. 5. Prediction equation of saturable glycine uptake was derived from experimental data. 6. Turning point and regression coefficients of segmented linear regression for the high affinity and low affinity components of glycine carrier were determined by using computer. 7. Ultrastructural changes of mucosal epithelial cells related to the experimental treatments were observed through transmission and scanning electron microscope.
( Chin-hee Song ),( Nayoung Kim ),( Sung Hwa Sohn ),( Sun Min Lee ),( Ryoung Hee Nam ),( Hee Young Na ),( Dong Ho Lee ),( Young-joon Surh ) 대한소화기학회 2018 Gut and Liver Vol.12 No.6
Background/Aims: Intestinal barrier dysfunction is a hallmark of inflammatory bowel diseases (IBDs) such as ulcerative colitis. This dysfunction is caused by increased permeability and the loss of tight junctions in intestinal epithelial cells. The aim of this study was to investigate whether estradiol treatment reduces colonic permeability, tight junction disruption, and inflammation in an azoxymethane (AOM)/dextran sodium sulfate (DSS) colon cancer mouse model. Methods: The effects of 17β-estradiol (E2) were evaluated in ICR male mice 4 weeks after AOM/DSS treatment. Histological damage was scored by hematoxylin and eosin staining and the levels of the colonic mucosal cytokine myeloperoxidase (MPO) were assessed by enzyme-linked immunosorbent assay (ELISA). To evaluate the effects of E2 on intestinal permeability, tight junctions, and inflammation, we performed quantitative real-time polymerase chain reaction and Western blot analysis. Furthermore, the expression levels of mucin 2 (MUC2) and mucin 4 (MUC4) were measured as target genes for intestinal permeability, whereas zonula occludens 1 (ZO-1), occludin (OCLN), and claudin 4 (CLDN4) served as target genes for the tight junctions. Results: The colitis-mediated induced damage score and MPO activity were reduced by E2 treatment (p<0.05). In addition, the mRNA expression levels of intestinal barrier-related molecules (i.e., MUC2, ZO-1, OCLN, and CLDN4) were decreased by AOM/DSS-treatment; furthermore, this inhibition was rescued by E2 supplementation. The mRNA and protein expression of inflammation-related genes (i.e., KLF4, NF-κB, iNOS, and COX-2) was increased by AOM/DSS-treatment and ameliorated by E2. Conclusions: E2 acts through the estrogen receptor β signaling pathway to elicit anti-inflammatory effects on intestinal barrier by inducing the expression of MUC2 and tight junction molecules and inhibiting pro-inflammatory cytokines. (Gut Liver 2018;12:682-693)
Chin-Hee Song,Na Young Kim,Ryoung Hee Nam,Soo In Choi,Changhee Kang,Jaeyoung Jang,Heewon Nho,신은,이하나 대한암예방학회 2021 Journal of cancer prevention Vol.26 No.1
Colon tumors develop more frequently in male than in female. Nuclear factor erythroid 2-related factor 2 (Nrf2) plays differential roles in the stage of tumorigenesis. The purpose of this study was to investigate the role of Nrf2 on colitis-associated tumorigenesis using Nrf2 knockout (KO) female mice. Azoxymethane (AOM) and dextran sulfate sodium (DSS)-treated wild-type (WT) and Nrf2 KO female mice were sacrificed at week 2 and 16 after AOM injection. Severity of colitis, tumor incidence, and levels of inflammatory mediators were evaluated in AOM/DSS-treated WT and Nrf2 KO mice. Furthermore, qRT-PCR, Western blot abnalysis, and ELISA were performed in colon tissues. At week 2, AOM/DSS-induced colon tissue damages were significantly greater in Nrf2 KO than in WT mice. At week 16, tumor numbers (> 2 mm size) were significantly lower in both the proximal and distal colon in Nrf2 KO compared to WT. The overall incidences of adenoma/cancer of the proximal colon and submucosal invasive cancer of the distal colon were reduced by Nrf2 KO. The mRNA and protein expression levels of NF-κB-related mediators (i.e., iNOS and COX-2) and Nrf2-related antioxidants (i.e., heme oxygenase-1 and glutamate-cysteine ligase catalytic subunit) were significantly lower in the Nrf2 KO than in WT mice. Interestingly, the protein level of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) was higher in AOM/DSS-treated Nrf2 KO than in WT mice. Our results support the oncogenic effect of Nrf2 in the later stage of carcinogenesis and upregulation of tumor suppressor 15-PGDH might contribute to the repression of colitis-associated tumorigenesis in Nrf2 KO female mice. Key Words Col
Sex-related Alterations of Gut Microbiota in the C57BL/6 Mouse Model of Inflammatory Bowel Disease
Hee Jin Son,김나영,Chin-Hee Song,Ryoung Hee Nam,Soo In Choi,김주성,이동호 대한암예방학회 2019 Journal of cancer prevention Vol.24 No.3
Background: Gut microbiota is closely associated with development and exacerbation of inflammatory bowel diseases (IBD). The aim of this study was to investigate differences in gut microbiota depending on sex and changes of gut microbiota during IBD developments. Methods: 16s rRNA metagenomic sequencing was performed for fecal materials from 8-week-old wild type (WT) and interleukin 10 (IL-10) knockout (KO) C57BL/6 mice of both sexes. Diversity indices, relative abundance of microbiota, and linear discriminant analysis effect size were examined to compare microbial communities between groups. Clustering of groups was performed by principal coordinates analysis (PCoA) and unweighted pair group method with arithmetic mean (UPGMA). Functional capabilities of microbiota were estimated using phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) based on Kyoto Encyclopedia of Genes and Genomes database. Results: PCoA and UPGMA tree analysis of beta-diversity demonstrated significant differences in gut microbiota between male and female groups of WT mice, but not of IL-10 KO mice. Firmicutes to Bacteroides ratio was higher in male group than that in female group in both WT mice and IL-10 KO mice. Phylum Proteobacteria significantly increased in female IL-10 KO mice than that in female WT mice. At species level, Lactobacillus murinus, Bacteroides acidifaciens, and Helicobacter hepaticus significantly increased in IL-10 KO mice than in WT mice. The relative abundance of beta-glucuronidase (K01195) was higher in female IL-10 KO mice than that in female WT mice by PICRUSt. Conclusions: Our results suggest that microbiota-host interactions might differ between sexes during development of IBD. (J Cancer Prev 2019;24:173-182)
Song, Chin-Hee,Kim, Nayoung,Lee, Sun Min,Nam, Ryoung Hee,Choi, Soo In,Kang, So Ra,Shin, Eun,Lee, Dong Ho,Lee, Ha-Na,Surh, Young-Joon Elsevier 2019 Biochemical pharmacology Vol.164 No.-
<P><B>Abstract</B></P> <P>Estrogen is known to have a protective effect in colorectal cancer (CRC) development. Previously, we reported the anti-inflammatory and antitumorigenic effects of 17β-estradiol (E2) in azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated male mice. The aim of this study was to investigate whether ovariectomy in a female AOM/DSS mouse model increases colorectal tumorigenesis and whether tumorigenesis is reduced by estrogen supplementation after ovariectomy. Clinical symptoms and histological severity of colitis and the levels of inflammatory mediators were evaluated in the colon of AOM/DSS-treated ovariectomized (OVX) mice. The levels of E2, myeloperoxidase (MPO), and NF-κB-dependent cytokines (interleukin (IL)-1β and IL-6) were measured by ELISA. Furthermore, quantitative real-time (qRT) PCR and Western blot analysis were performed. Ovariectomy did not aggravate AOM/DSS-induced colitis at 2 weeks. At weeks 10 and 16, ovariectomy significantly increased tumor number and incidence rate in only the proximal colon after AOM/DSS treatment (F_AOM/DSS vs OVX_AOM/DSS), and these increases were significantly reduced by E2 supplementation (OVX_AOM/DSS vs OVX_AOM/DSS/E2). However, ovariectomy did not affect CRC development in the distal colon (F_AOM/DSS vs OVX_AOM/DSS). At week 2, E2 administration to AOM/DSS-treated OVX mice attenuated the histological severity of colitis by decreasing the protein and/or mRNA levels of estrogen receptor alpha (ERα) and NF-κB-related mediators (i.e., COX-2, TNF-α, and IL-6) and by enhancing estrogen receptor beta (ERβ) and nuclear Nrf2 protein expression and the mRNA expression of related antioxidant enzyme genes (i.e., HO-1, GCLC, GCLM, and NQO1). Endogenous estrogen in females protects against the development of proximal colon cancer, and exogenous E2 replacement in OVX female mice showed protective effects against AOM/DSS-induced colitis and carcinogenesis. The mechanism could involve modulating ERs-, NF-κB- and Nrf2-mediated pathways.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Structure-based Virtual Screening and Identification of a Novel Androgen Receptor Antagonist
Song, Chin-Hee,Yang, Su Hui,Park, Eunsook,Cho, Suk Hee,Gong, Eun-Yeung,Khadka, Daulat Bikram,Cho, Won-Jea,Lee, Keesook American Society for Biochemistry and Molecular Bi 2012 The Journal of biological chemistry Vol.287 No.36
군병원 중환자실 병원 감염률 추이분석과 민간병원 병원감염률 및 항생제 민감성 비교
송주희 ( Ju-hee Song ),이희경 ( Hee-kyung Lee ),윤선영 ( Sun-young Yoon ),남은혜 ( Eun-hye Nam ),오유리 ( Yu-ri Oh ),이진국 ( Chin-kook Rhee ) 국군의무사령부 2011 대한군진의학학술지 Vol.42 No.1
BACKGROUND: The purpose of this study was to exam the nosocomial infection rate of intensive care unit (ICU) in military hospital and to compare with civilian hospitals and past rate in military hospital. METHODS: We retrospectively surveyed the nosocomial infection rate of ICU in Armed Forces Capital Hospital (AFCH) between 2005 to 2010. We compared infection rate with the data of Korean Nosocomial Infections Surveillance System (KONIS) and AFCH in 2000. RESULT: Total 47 cases of nosocomial infections were detected during the study period. Among them, 15 cases were pneumonia, 9 were blood stream infection, and 17 were urinary tract infection. The infection rate (2.12) was significantly lower than AFCH in 2000 (15.48, P < 0.0001) and KONIS (8.40, P < 0.0001). The rate of imipenem resistant Acinetobacter was also significantly lower than KONIS (33.3% vs 82.5%, AFCH vs KONIS; P = 0.012). CONCLUSION: The nosocomial infection rate in ICU of AFCH is significantly lower than KONIS and AFCH in 2000. Further study about the nosocomial infection rate of military hospital is needed.