전립선암 환자에서 Insulin-Like Growth Factor I과 변이지수의 관계

천성엽,황평한,김형진 大韓泌尿器科學會 2003 Korean Journal of Urology Vol.44 No.3

지구성 운동이 쥐 혈청 IGF-1, IGFBP-3 수준과 골격근 형태별 GLUT-4에 미치는 영향

육현철(Yug Hyeon Cheol),조준용(Jo Jun Yong),오유성(O Yu Seong) 한국사회체육학회 2003 한국사회체육학회지 Vol.20 No.2

We investigate the effects of 12wk treadmill exercise on the skeletal muscle GLUT-4 protein, serum glucose, insulin, IGF-1, IGFBP-Ⅲ and lipids in the insulin resistant obese Zucker rats(fa/fa). Male obese Zucker rats either remained sedentary(fa/fa, n=7), and performed treadmill exercise(fa/fa, n=7) for 12wk. During oral glucose tolerance test(OGTT), treadmill exercise resulted in a significant lowering of the glucose, insulin and TC, TG, LDL-C responses compared with sedentary controls. Treadmill exercise increased serum IGF-1, IGF-BP-III and insulin-regulated glucose transporter(GLUT-4) in isolated soleus, plantaris muscle. The greatest expressions in GLUT-4 protein in these muscles were observed in the treadmill exercise group. Thus, enhancement in glucose transport in soleus and plantaris muscle due to treadmill exercise was related to enhanced serum IGF-1, IGF-BP-III and GLUT-4 protein and decrease glucose, insulin and TC, TG, LDL-C. These results suggest that treadmill exercise may reduce the risk of associated diseases including diabetes and coronary heart disease.

Serum and seminal plasma insulin-like growth factor-1 in male infertility

Lee, Hyo Serk,Park, Yong-Seog,Lee, Joong Shik,Seo, Ju Tae The Korean Society for Reproductive Medicine 2016 Clinical and Experimental Reproductive Medicine Vol.43 No.2

Objective: Growth hormone and its mediator, insulin-like growth factor-1 (IGF-1), have been suggested to exert gonadotropic actions in both humans and animals. The present study was conducted to assess the relationship between serum IGF-1 concentration, seminal plasma concentration, and sperm parameter abnormalities. Methods: A total of 79 men were enrolled in this study from December 2011 to July 2012 and were prospectively analyzed. Patient parameters analyzed included age, body mass index, smoking status, urological history, and fertility history. Patients were divided into four groups based on their semen parameters: normal (A, n=31), abnormal sperm motility (B, n=12), abnormal sperm morphology (C, n=20), and two or more abnormal parameters (D, n=16). Patient seminal plasma and serum IGF-1 concentrations were determined. Results: Patient baseline characteristics were not significantly different between any of the groups. The serum IGF-1 levels in groups B, C, and D were significantly lower than the levels in group A; however, the seminal plasma IGF-1 levels were not significantly different between any of the groups. Conclusion: Men with abnormal sperm parameters had significantly lower levels of serum IGF-1 compared with men with normal sperm parameters. Seminal plasma IGF-1 levels, however, did not differ significantly between the groups investigated here. Further investigations will be required to determine the exact mechanisms by which growth hormone and IGF-1 affect sperm quality.

Optimal Milieu for Culturing Porcine Sertoli Cell

Md, Anower Jabed,Tania Kamel,Byung-Ki Kim 한국동물생명공학회(구 한국동물번식학회) 2006 Reproductive & developmental biology Vol.30 No.3

The purpose of the present study was to establish culture conditions for the in vitro study of the neonatal piglet Sertoli cell. Isolation for the culture of Sertoli cell was established using collagenase and pancreatin digestion of testicular tissues. The effects of various culture media, fetal bovine serum (FBS), follicular stimulating hormone (FSH), epidermal growth factor (EGF) and insulin-transferrin-sodium selenite (ITS) on growth of neonatal piglet Sertoli cells were investigated. The mitogenic effects of Dulbecco’s modified Eagle’s medium + Ham’s F-12 medium was higher than other media used in this experiment. The addition of 1% FBS in cultures was necessary for attachment of Sertoli cell clusters. However, except FBS and EGF, FSH and ITS did not stimulate Sertoli cell proliferation. When Sertoli cells isolated from neonatal piglets were cultured in Dulbecco’s modified Eagle’s medium + Ham’s F-12 medium supplemented with 1% FBS, FSH, EGF and ITS, the yield and plating efficiency of Sertoli cells were largely increased. Confluency of Sertoli cells was reached as early as 4 days of culture. The method described here reduces or eliminates many of the drawbacks of the conventional procedures used to isolate and culture of Sertoli cells, thus providing a useful tool in studies of growth kinetics and regulation of cell proliferation in vitro.

Optimal Milieu for Culturing Porcine Sertoli Cell

Jabed Md. Anower,Kamal Tania,Kim, Byung-Ki The Korean Society of Animal Reproduction 2006 Reproductive & developmental biology Vol.30 No.3

The purpose of the present study was to establish culture conditions for the in vitro study of the neonatal piglet Sertoli cell. Isolation for the culture of Sertoli cell was established using collagenase and pancreatin digestion of testicular tissues. The effects of various culture media, fetal bovine serum(FBS), follicular stimulating hormone(FSH), epidermal growth factor(EGF) and insulin-transferrin-sodium selenite(ITS) on growth of neonatal piglet Sertoli cells were investigated. The mitogenic effects of Dulbecco's modified Eagle's medium+Ham's F-12 medium was higher than other media used in this experiment. The addition of 1% FBS in cultures was necessary for attachment of Sertoli cell clusters. However, except FBS and EGF, FSH and ITS did not stimulate Sertoli cell proliferation. When Sertoli cells isolated from neonatal piglets were cultured in Dulbecco's modified Eagle's medium+Ham's F-12 medium supplemented with 1% FBS, FSH EGF and ITS, the yield and plating efficiency of Sertoli cells were largely increased. Confluency of Sertoli cells was reached as early as 4 days of culture. The method described here reduces or eliminates many of the drawbacks of the conventional procedures used to isolate and culture of Sertoli cells, thus providing a useful tool in studies of growth kinetics and regulation of cell proliferation in vitro.

PAM-1616, a selective peroxisome proliferator-activated receptor γ modulator with preserved anti-diabetic efficacy and reduced adverse effects

Kim, M.K.,Chae, Y.N.,Choi, S.h.,Moon, H.S.,Son, M.H.,Bae, M.H.,Choi, H.h.,Hur, Y.,Kim, E.,Park, Y.H.,Park, C.S.,Kim, J.G.,Lim, J.I.,Shin, C.Y. North-Holland ; Elsevier Science Ltd 2011 european journal of pharmacology Vol.650 No.2

Peroxisome proliferator-activated receptor (PPAR) γ is known to be a key regulator of insulin resistance. PAM-1616 is a novel, non-thiazolidinedione small molecule compound synthesized in Dong-A Research Center. In this study, we characterized the pharmacological and safety profiles of PAM-1616 as a selective PPARγ modulator. PAM-1616 selectively binds to human PPARγ (IC<SUB>50</SUB>, 24.1+/-5.6nM) and is a partial agonist for human PPARγ with an EC<SUB>50</SUB> of 83.6+/-43.7nM and a maximal response of 24.9+/-7.1% relative to the full agonist, rosiglitazone. PAM-1616 was selective for human PPARγ than for human PPARα (EC<SUB>50</SUB>, 2658+/-828nM) without activating human PPARδ, which makes it a selective modulator of PPARγ. Treatment of high fat diet-induced obese C57BL/6J mice with PAM-1616 for 21days improved HOMA-IR. Furthermore, PAM-1616 significantly improved hyperglycemia in db/db mice with little side effect when orally administered at a dose of 1mg/kg/day for 28days. Intriguingly, PAM-1616 was seen to increase the gene expression of inducible glucose transporter (GLUT4), while it partially induced that of a fatty acid carrier, aP2 in 3T3-L1 adipocytes, and it also showed partial recruitment of an adipogenic cofactor, TRAP220 as compared to rosiglitazone. PAM-1616 did not cause a significant increase in plasma volume of ICR mice when orally administered at a dose of 10mg/kg/day for 9days. PAM-1616 increased the expression of fluid retention-inducing genes such as serum/glucocorticoid-regulated kinase (SGK)-1 to a lesser extent as compared to rosiglitazone in human renal epithelial cells. These results suggest that PAM-1616 acts as a selective modulator of PPARγ with excellent antihyperglycemic property. The differential modulation of target gene by PAM-1616 might contribute to the improved side effect profiles.

Association between Serum Albumin, Insulin Resistance, and Incident Diabetes in Nondiabetic Subjects

배지철,서성환,허규연,김재현,이명식,이문규,이원영,이은정,오기원 대한내분비학회 2013 Endocrinology and metabolism Vol.28 No.1

Background: Serum albumin has been suggested to be associated with insulin resistance. We evaluated the association between serum albumin concentration and insulin resistance. We also investigated whether serum albumin level has an independent effect on the development of diabetes. Methods: In our study, 9,029 subjects without diabetes, who underwent comprehensive health check-ups annually for 5 years,were categorized into tertiles based on their serum albumin levels at baseline. The odds ratio (OR) for the prevalence of insulin resistance, defined as the top quartile of homeostasis model assessment of insulin resistance and the presence of impaired fasting glucose and nonalcoholic fatty liver disease, was evaluated cross-sectionally. Also, the hazard ratio (HR) for incident diabetes was estimated longitudinally, according to the baseline albumin tertiles using Cox proportional hazard analysis respectively. Results: From the lowest to the highest tertile of albumin, the multivariable-adjusted ORs of insulin resistance increased significantly in both men and women. During the mean follow-up period of nearly 4 years, 556 (6.1%) subjects progressed to diabetes. The multivariable-adjusted HR (95% confidence interval [CI]) of diabetes in men were 1, 1.09 (95% CI, 0.86 to 1.40), and 1.10(95% CI, 0.86 to 1.41), respectively, from the lowest to the highest tertiles of baseline albumin. Corresponding values for women were 1, 1.21 (95% CI, 0.66 to 2.21), and 1.06 (95% CI, 0.56 to 2.02), respectively. Conclusion: Our study showed that increased serum albumin level was associated with insulin resistance. However, serum albumin did not have an independent effect on the development of diabetes.

Elevated Serum Insulin is an Independent Risk Factor for Hepatocellular Carcinoma: A Case Control Study from Nepal

Gupta, Satrudhan Pd,Mittal, Ankush,Sathian, Brijesh,Jha, Dipendra Kumar Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Aim: To investigate associations of fasting insulin and glucose levels in serum with hepatocellular carcinoma risk. Materials and Methods: This hospital based study was carried out using data retrieved from the register maintained in the Department of Biochemistry of the Nepalese Army Institute of Health Sciences, between 1st December, 2011 and 31st June, 2013. The variables collected were age, fasting plasma glucose, fasting plasma insulin and ALT. Quantitative determination of human insulin concentrations was accomplished by chemiluminescence enzyme immunoassay. Results: Of the total 220 subjects enrolled in our present study, 20 cases were of HCC and 200 were healthy controls. The maximum number of cases of hepatocellular carcinoma in category cutpoints of fasting insulin levels fell in the range of >6.10 ${\mu}U/ml$. The highest insulin levels (>6.10 ${\mu}U/ml$) were seen to be associated with an 2.36 fold risk of HCC when compared with fasting insulin levels of (<2.75 ${\mu}U/ml$). Furthermore, the insulin levels (2.75-4.10 ${\mu}U/ml$) of category cutpoints also conferred a 1.57 fold risk for HCC when compared with lowest fasting insulin levels of (<2.75 ${\mu}U/ml$). Conclusions: The effect of an insulin level in increasing HCC risk appeared consistent, influencing incidence, risk of recurrence, overall survival, and treatment-related complications in HCC patients.

Omega-3 Fatty Fish의 섭취가 정상인의 Serum Insulin, Glucose에 미치는 영향

김영선 한국식품영양학회 1995 韓國食品營養學會誌 Vol.8 No.4

Marine ω-3 fatty fish가 정상인의 공복 혈당 및 기저 인슐린 농도에 미치는 영향을 살펴본 결과는 다음과 같다. Low ω-3 fatty acid군은 3.8g ω-3 fatty acid (EPA 1.0g, DHA 2.8g)를 포함, 약 1,780kcal/day의 실험식을, high ω-3 fatty acid군은 7.7g ω-3 fatty acid(EPA 2.06g, DHA 5.68g)가 함유된 약 1,815kcal/day의 실험식을 각각 1주일씩 투여받았으며, 실험 후 체중과 BMI는 두 군 모두에서 약간의 증가를 보였으나, 실험 전, 후의 체중 및 BMI가 모두 정상 범위였다. 공복 혈당농도는 low ω-3 fatty acid군이 실험 전 89.8±7.0mg/dl에서 88.7±8.5mg/dl로, high ω-3 fatty acid군은 91.0±6.6mg/dl에서 89.8±6.7mg/dl로 두 군 모두 약간 감소하였으나, 유의성은 없었으며, 두 군간에 ω-3 fatty acid 투여량에 따른 유의적 차이는 나타나지 않았다. 기저 인슐린 농도는 low ω-3 fatty acid군이 4.02±1.44uU/ml에서 4.54±2.61uU/ml 약 12.9% 정도 증가하고, high ω-3 fatty acid군은 4.97±2.13uU/ml에서 5.19±2.19uU/ml로 약간 증가하였으나, 두 군 모두 유의성은 없었으며, 두 군간에 유의적 차도 존재하지 않았다. 이로 미루어 marine ω-3 fatty acid 투여는 인슐린 비의존형 당뇨병환자에 미치는 영향과는 달리, 정상인에 있어서는 일상적 섭취수준이나 그 보다 비교적 높은 섭취수준에도 glycemic control에 그다지 영향이 미치지 않을 것으로 사료된다. The purpose of this study was to investigate the response of fasting serum glucose and basal insulin to dietary omega-3 fatty fish in normal subjects. Nineteen healthy female volunteer subjects were divided into two groups, depending on fish preference test. Low ω-3 fatty acid group for 7 days received a experimental diet containing mackeral fish 100g. Calorie intake was 1780 kcal/day. The average daily ω-3 fatty acid consumption from fish was 3.87g/day (1.03g EPA, 2.84g DHA). High ω-3 fatty acid group was given 7.74g maine ω-3 fatty aicd (200g mackeral fish) consisting of 2.06g EPA, 5.68g DHA. Calorie intake was 1815 kcal/day. Fasting blood serum glucose, insulin levels were measured at baseline, 7days after experimental diet. In the beginning the levels of fasting serum glucose, basal insulin were not different between both groups. There were no significant changes in fasting serum glucose, insulin levels by experimental diets. These data indicate that marine ω-3 fatty acid consumption have no deleterious effect on glycemic control in normal subjects.

천연 생약 복합 추출물이 Streutozotocin 유발 당뇨성 흰쥐의 혈당 및 인슐린, 혈청 과산화지질 농도에 미치는 효과

박형래,조정순,Park, Hyung-Rea,Cho, Jung-Soon 동아시아식생활학회 2007 동아시아식생활학회지 Vol.17 No.2

This study was designed to investigate a natural medicinal multi-plants extract (BG515), which consisted of multi extracts of Mori folium, Rehmannia glutinosa Liboschitz, Dioscorea japonica, Lycii fructus, and Astragalus radix, on blood glucose, insulin levels, and serum malondialdehyde (MDA) concentrations in streptozotocin-induced diabetic rats. Streptozotocin (STZ) induces a type 1 diabetes mellitus in rats. Type 1 is usually characterized by the presence of islet cell autoantibodies (ICA), autoantibodies to insulin (IAA), and autoantiboides to glutamic acid decarboxylase (GAD), which identify the autoimmune process that leads to $\beta-cell$ destruction. Thirty-five male Sprague-Dawley (SD) rats weighing $150\sim170g$ each (6 weeks old) were randomly divided into one control (Group A) and 4 STZ-induced diabetic groups, and were subjected to one of the following treatment for 12 weeks. Groups A and B were fed basal diets and Group C, D, and E received the same diets as groups A and B, but with supplements of 150 mg/kg, 300 mg/kg, and 600 mg/kg of BG515 orally for 12 weeks, respectively. Diabetes was induced in Groups B, C, D, and E by intravenous injection of 45 mg/kg of STZ per body weight in sodium citrate buffer (pH 4.5) via the tail vein. In the BG515 groups, we found increases in serum insulin levels, compared to the STZ-control group, but these data were not significant. In contrast, blood glucose and serum MDA concentrations decreased in the BG515 groups compare to the STZ-control group. At the 5th week, in all the BG515 administered groups, there were decreases in serum blood glucose levels compared to the STZ- control group, and this activity was very strong in the BG515-1 group at the 12th week. These results suggest that natural bio-complex compounds (BG515) may slightly suppress STZ-induced changes in serum MDA concentration via the maintenance of serum insulin levels, due to the prevention of $\beta-cell$ and glucagon destruction by STZ.