키토산올리고당이 사람 제대정맥 내피세포에서 Nitric Oxide 생성에 미치는 영향

최호진 ( Ho Jin Choi ),김영호 ( Young Ho Kim ) 한국키틴키토산학회 2011 한국키틴키토산학회지 Vol.16 No.4

Most adult-onset cardiovascular diseases are associated with age-related vascular dysfunction which is mainly a result of reduced NO production in endothelial cells. It was investigated that the effects of chitosan oligosaccharide (COS) on age-related vascular dysfunction. To this purpose, the antioxidant activity of COS, that is, the reduction of DPPH radicals and the inhibition of lipid peroxidation were measured. And the change in NO production content in cultured human umbilical vein endothelial cells were also measured. COS showed good antioxidant activity, that is, the reduction of DPPH radicals and the inhibition of lipid peroxidation. And chitosan oligosaccharide also increased the NO production. In this conclusion, COS was a good antioxidant and would have a protective effect on vascular dysfunction via increasing NO production in endothelial cells.

Effect of Reactor Surface on Production of Bacterial Cellulose and Water Soluble Oligosaccharides by Gluconacetobacter hansenii PJK

나사룰라,하정환,박중곤 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.1

The effect of agar plates on the bacterial cellulose (BC) production in a static culture was investigated in order to find the role of agar component as a surface modifying agent. Two types of surface modified reactors (SMRs: SMRD and SMRB) were prepared by coating the bottom of the reactors with agar dissolved in distilled water and basal medium, respectively. The SMRs were used for BC and water soluble oligosaccharides (WSOS) production. Control was done by the same procedure using reactors without agar plate. In both types of SMRs, the maximum production rate was observed after the second day of cultivation compared to third day of cultivation in the case of the control. The maximum productions of BC 5.308 and 5.472 g/L were observed at the first batch using SMRs prepared with agar dissolved in distilled water (SMRDs) and SMRs prepared with agar dissolved in a basal medium (SMRBs), respectively. Similarly, in the daily-culture and successive batch strategy experiments the maximum amount of WSOS produced in the SMRs was almost double that of the control. The highest water holding capacity value 92.21 g/g was observed for BC formed in the SMRs prepared with 3.0% of agar. FTIR and XRD analyses were carried out to study the structural features of the prepared BC.

Effects of chitosan oligosaccharide and hyriopsis cumingii polysaccharide on the quality of wheat flour and extruded flour products

Yuan Ke,Beibei Ding,Yang Fu,Miaomiao Zhang,Shensheng Xiao,Wenping Ding,Heng Yang,Qingyun Lv,Zhuo Zheng,Xuedong Wang 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.7

Effects of chitosan oligosaccharide (COS) andhyriopsis cumingii polysaccharide (HCP) on the quality ofwheat flour and corresponding extruded flour productswere investigated in this work. The results showed thatboth COS and HCP are conducive to the improvement ofdough quality. Moreover, compared to control groupsamples, the moisture content, expansion ratio and oilabsorption rate of the samples were increased and thehardness were decreased with the addition of COS. Thesephenomena indicate the quality of extruded flour productsbecame better in the presence of COS as well. However,HCP has little or no effect on the quality of extruded flourproducts may be due to its degradation under high temperatureand pressure extrusion. COS with higher stabilityexhibited better improvement effects on the quality ofextruded flour products and showed a promising prospectfor application in extruded food industry.

Glucansucrases를 이용한 새로운 올리고당의 합성

백진숙,김도만,이진하,장판식,한남수,JohnF.Robyt 한국산업미생물학회 1998 한국미생물·생명공학회지 Vol.26 No.2

본 연구에서는 dextransucrase hyper-producing Leuconostoc mesenteroides B-512FMCM과 dextransucrase constitutive mutants인 B-742CB 및 B-1355C에서 얻은 dextransucrase 및 alternansucrase를 이용하여 maltose, lactose, gentiobios 및 raffinose의 수용체반응을 수행하였다. B-512FMCM의 경우 수용체로서 maltose와 gentiobiose는 설탕에 대한 농도비가 1/1이하일 때 수용체에 α1-6으로 D-glucose가 결합한 일련의 올리고당을 생성하였고, 9/1이상에서는 단 두 가지의 수용체산물로서 maltose의 경우에는 panose와 isomaltosyl maltose를, gentiobiose의 경우에는 6^2-α-D-glucopyranosyl gentiobiose와 isomaltosyl gentiobiose만을 생성하였다. 그러나 lactose는 유일한 산물인 2^1-α-D-glycopyranosyl lactose만을 생성하였다. 효소양의 변화에 대해서 maltose와 gentiobiose의 수용체반응에서 생성된 수용체산물(올리고당)의 수와 양은 효소양이 증가함에 따라 증가하였으나 lactose의 경우에는 효소양의 증가에 대해 수용체산물 양의 변화가 크지않았다. B-742CB dextransucrase와 B-1355C alternansucrase를 사용하면 B-512FMCM dextransucrase에서는 단 한가지만의 수용체산물을 생산하였던 lactose와 raffinose를 수용체로하여 여러 가지의 수용체산물(올리고당)을 생산하였다. Dextransucrase hyper-producing Leuconostoc mesenteroides B-512FMCM and dextransucrase constitutive mutants B-742CB and B-1355C catalyzed the transfer of glucose from sucrose to other carbohydrates which were present or were added to the reaction digests. When the acceptor was a maltose, gentiobiose, lactose or raffinose, there was produced a series of oligosaccharide acceptor products or single product based on the kinds of enzymes and reaction conditions. To obtain the quantitative information about the yield and the distribution of acceptor products and dextran two experimental parameters were studied: a) the ratio of acceptor to sucrose and b) the amount of enzyme at constant carbohydrate concentration (100 mM). As the amount of enzyme increased, the synthesis of acceptor products (of maltose or gentiobiose) increased, and the formation of dextran decreased. As the ratio of acceptor to sucrose increased, the amount of dextran and the number of acceptor-products decreased and the amount of acceptor-products increased. When maltose or gentiobiose was an acceptor, the glucose from sucrose was transferred to the C-6 hydroxyl group of the nonreducing-end glucose residue of acceptors to give a homologous series of isomaltosyl dextrins. In case of lactose or raffinose, there was produced only one acceptor product from B-512FMCM dextransucrase reaction. In the lactose acceptor reaction, the glucose from sucrose was transferred to the C-2 hydroxyl of the reducing end glucose residue of lactose. To get a series of oligosaccharides from lactose or raffinose acceptor reaction we used B-742CB dextransucrase or B-1355C alternansucrase with 500 mM sucrose in reaction digest.

갈락토올리고당 생산 효모 Bullera singularis의 최적 배양조건

신현재,박오진,양지원 한국산업미생물학회 1995 한국미생물·생명공학회지 Vol.23 No.5

갈락토올리고당 생산 효모인 Bullera singularis의 배양조건을 최적화 하였다. 최적 배양온도는 25℃, pH는 6.0, 초기 접종농도 5%(v/v) 이상, 초기 유당농도 5%(v/w) 이상이었다. 미생물의 성장과 갈락토올리고당의 생산은 비례하였으며 5% 유당과 0.75% yeast extract를 함유한 배지에서 배양할 경우 배양 70시간만에 배지내에 고형분 기준으로 72%의 갈락토올리고당을 생산할 수 있었다. 생산된 올리고당은 2당류 3가지, 3당류 2가지, 4당과 5당이 각기 한가지씩이었으며, 탄소원의 대사작용을 고려하여 B. singularis에 의한 갈락토올리고당 생산 메커니즘을 새로이 제안하였다. The cultural conditions of Bullera singularis were optimized for the efficient production of galactooligosaccharide (GOS). Optimum temperature was 25℃, pH was 6.0, inoculum size was over 5% (v/v), initial lactose concentration was over 5% (w/v). The GOS production increased with microbial growth. Maximum amount of 72% (w/w) GOS was obtained from the optimized medium (5% lactose and 0.75% yeast extract) in 70 hours. Seven types of GOS (3 of dimer, 2 of trimer, 1 of tetramer, and 1 of pentamer) were identified by two-dimensional TLC. A new mechanism of GOS production is proposed based on the metabolism of carbon source.

Physiochemical Characterization of Various Pear Varieties and their Practical Application for Industrialization

Seung-Hee NAM,Sun-Hee YIM,Jong-Bang EUN 한국생물공학회 2014 한국생물공학회 학술대회 Vol.2014 No.4

Detailed Modes of Action and Biochemical Characterization of endo-Arabinanase from Bacillus licheniformis DSM13

박정미,장명운,강정현,김민정,이소원,송영복,신철수,한남수,김태집 한국미생물학회 2012 The journal of microbiology Vol.50 No.6

An endo-arabinanase (BLABNase) gene from Bacillus licheniformis DSM13 was cloned and expressed in Escherichia coli, and the biochemical properties of its encoded enzyme were characterized. The BLABNase gene consists of a single open reading frame of 987 nucleotides that encodes 328 amino acids with a predicted molecular mass of about 36 kDa. BLABNase exhibited the highest activity against debranched α-(1,5)-arabinan in 50 mM sodium acetate buffer (pH 6.0) at 55°C. Enzymatic characterization revealed that BLABNase hydrolyzes debranched or linear arabinans with a much higher activity than branched arabinan from sugar beet. Enzymatic hydrolysis pattern analyses demonstrated BLABNase to be a typical endo-(1,5)-α-L-arabinanase (EC 3.2.1.99) that randomly cleaves the internal α-(1,5)-linked L-arabinofuranosyl residues of a branchless arabinan backbone to release arabinotriose mainly, although a small amount of arabino-oligosaccharide intermediates is also liberated. Our results indicated that BLABNase acts preferentially along with the oligosaccharides longer than arabinopentaose, thus enabling the enzymatic production of various arabinooligosaccharides.