Effect of Several Growth Factors on Hair Follicle Growth in Hair Follicle Organ Culture

Kim, Jeong Aee,Eun, Hee Chul Korean Dermatological Association 1994 Annals of Dermatology Vol.6 No.1

In vivo and In vitro hair growth promotion effects of extract from Glycine soja Siebold et Zucc

( Jae Chan Yang ),( Bo Ae Kim ) 한국응용생명화학회 2016 Journal of Applied Biological Chemistry (J. Appl. Vol.59 No.2

Hair is a dermal adjunctive organ that protects the body from external physical and chemical stimuli; hair undergoes anagen, catagen, and telogen phases, with hair-loss occurring during the telogen phase. Alopecia is a condition wherein a person undergoes hair-loss far exceeding the normal amount, owing to diverse external factors. Wild beans are rich in isoflavone and amino acids known to prevent hair-loss; compared to cultivated beans, many wild bean species have higher protein content. This study aimed to develop a hair growth promoting solution, with superior hair growth promoting effects and fewer side effects, using naturally obtained Glycine soja Siebold et Zucc (GSSZ) extracts. Sevenweek- old C57BL/6N male mice were classified into different experimental groups. Hair growth was observed in GSSZ-treated mice, and compared against that seen in 3 % minoxidil (MXD, positive control)-treated mice. Visual observations revealed a greater reduction in hair-loss in MXD and GSSZ application groups, compared to that in TXN group (hair loss induction using 1 % testosterone). Evaluation using an image analysis software revealed that compared to the positive control, TXN + GSSZ group showed the highest hair growth. TXN + MXD and control groups exhibited similar follicular cell growth, while the hair growth promotion patterns were similar in the negative control (normal), TXN + GSSZ, and TXN groups, as observed via histological analysis. GSSZ did not induce cytotoxicity (even at 2 mg/mL) in keratinocytes and dermal papilla cells; alternately, dermal papilla cell proliferation was activated in a (GSSZ) concentration-dependent manner. Therefore, the GSSZ extract promoted hair growth and increased hair growth-related cell activity, and could therefore be utilized in alopecia treatment.

천연추출물을 이용한 발모 및 두피 개선에 관한 연구 동향

김경숙 한국피부과학연구원 2014 대한피부미용학회지 Vol.12 No.1

These days, the population who is suffering from hair loss resulted from variation of genetic, social, and cultural factors hasincreased. However, according to researches, medicinal therapy accompanies demerits of various side effects and requires totake a long period of time. Therefore, for mineralizing the demerits, lots of researches have engaged with application of naturalobjects. This research aims not only to explore research trends relevant with hair growth and scalp improvement by applyingsafe natural extracts but also to suggest a basic material which reports natural extracts having hair growth promotion effect. Among aroma essential oils, hair growth promotion effect can be found in a peppermint and a rosemary. Futhermore, thepeppermint shows an excellent effect against dandruff bacillus. Also the promotion effect can be found in sandalwood and roseabsolute, which is proved by hair production and increased number of hair follicle. Among plant extracts, the promotion effectcan be found in polygoni mulitiflori radix water extracts, which is proved by thickened thickness of dermis, increased numberof hair follicle, deepen depth of hair follicle. Applying wild cultivated ginseng results in increased number of hair, thickenedthickness of hair, as well as enhancement of hair density. The wild cultivated ginseng plays a prevention role against death ofhair follicle cells and play a promotion role to regeneration of that. And the general ginseng plays a promotion role for hair cellgrowth. Wild ginseng roots extracts has hair growth promotion effect and presents high manifestation of VEGF, IGF-1, besideshas an anti-bacterial effect against dandruff bacillus. Sparassis crispa presents hair growth promotion in the group of waterfraction of sparassis crispa extract, which means there is increased number and deepen depth of hair follicle. A herbral product(DCS-HT®), which is composed of mulberry root extract and polygoni mulitiflori radix water extracts and β-sitosterol at the 1:1:3proportion, presents hair growth promotion effect, activation of ALP, γ-GT enzyme, and high manifestation of IGF-1, TGF-β. Applying acetone extract makes hair growth in the EtoAc fractionation and hair follicles occurrence in the hypoderma. Applyingphellinus linteus extract leads to reduction of testosterone and DHT consideration in blood and increase of hair growth. There isa demand for further exploration whose purpose is to seek extracts having ability to promote hair growth effect and fewer sideeffect. One area that also needs further exploration is to mix or combine the extracts known as having such effect.

어성초를 포함한 천연복합추출물의 C57BL/6 마우스에 대한 발모효과

홍양희,배송환,서형주 대한피부미용학회 2015 대한피부미용학회지 Vol.13 No.3

Present study was carried out to investigate the effect of herbal complex extract including Houttuynia cordata Thunb on hair growth and gene expression in C57BL/6 mice. Twenty mice were divided into 4 experimental groups including normal (control), 50% herbal complex extract (S-1), 100% herbal complex extract (S-2) and 5% minoxidil-treated group. The test materials were daily applied with 200 μl per mouse on shaved dorsal skin for 3 weeks. The hair growth was monitored by photograph at 7, 14 and 21 days after topical application. Then the changes of hair density and hair thickness in the hair-removed area were evaluated by phototrichogram using folliscope. Also the expression level of growth factors related to hair growth was measured by real time polymerase chain reaction. Application of S-2 or minoxidil-treated group stimulated the hair growth compared to vehicle treatment. Therefore hair density of S-2 or minoxidiltreated group application was increased about 2.5 and 3.1 fold more than in vehicle application on 21 day, respectively. And hair weight of both S-2 and minoxidil-treated group were increased about 8.3 and 10.0 fold more than in vehicle spreading on 21 day, respectively. Futhermore the gene expression of insulinlike growth factor (IGF)-1 and vascular endothelial growth factor (VEGF) were significantly up-regulated in S-2 and minoxidil-spreaded group compared to vehicle-applied group. However the gene expression of transforming growth factor (TGF)-β1 was not significantly down-regulated in S-2 and minoxidil-spreaded group compared to vehicle-applied group. These data suggest that S-2 has potent stimulating activity on hair growth in C57BL/6 mice and potential usefulness as ingredients of hair tonic and hair restore.

Hair-growth-promoting effect of conditioned medium of high integrin α6 and low CD 71 (α6bri/CD71dim) positive keratinocyte cells

( Young Jae Kim ),( Chong Hyun Won ),( Yun Mi Jeong ),( Sangjin Kang ),( Tae Sung Koo ),( So Hyun Park ),( Ki Young Park ),( Young Kwan Sung ),( Jong Hyuk Sung ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Keratinocyte stem/progenitor cells (KSCs) reside in the bulge region of the hair follicles and may be involved in hair growth and regeneration. Objectives: We studied the hair growth promotion effects of KSC-CM on human HF organ culture and on a C3H/HeN mouse model. We then investigated the proliferative effect of KSC-CM on both human hair follicle dermal papilla cells (HFDPCs) and outer root sheath (ORS) cells. Methods: Hair follicle dermal papilla cells (HFDPCs) and outer root sheath (ORS) cells were treated with conditioned medium (CM) of KSCs. Moreover, the effects of KSC-CM on hair growth were examined ex vivo and in vivo. A human growth factor chip array and RT-PCR were employed to identify enriched proteins in KSC-CMas compared with CM from keratinocytes. Results: KSC-CM increased the proliferation of HFDPCsand ORS cells, and increased the S-phase of the cell cycle in HFDPCs. After subcutaneous injection of KSC-CM in C3H/HeN mice, a significant increase in hair growth and increased proliferation of hair matrix keratinocytes ex vivo was observed. We identified six proteins enriched in KSC-CM. A growth-factor cocktail that contains these six recombinant growth factors significantly increased the proliferation of HFDPCs and ORS cells and enhanced the hair growth of mouse models. Conclusion: These results collectively indicate that KSC-CM has the potential to increase hair growth via the proliferative capacity of HFDPCs and ORS cells.

마우스모델을 이용한 황산아연 및 염화아연의 발모 효과

민명자,최문희,신현재 대한피부미용학회 2015 대한피부미용학회지 Vol.13 No.4

Hair medically helps to protect scalp, maintain body temperature, emit heavy metals and so on. Aesthetically, while, it is important to express ones confidence through beautiful decoration of its style. Recently, therefore, hair loss and/or alopecia have received a lot of attention from the public as well as medical doctors and scientists. In order to treat alopecia and to improve the hair loss symptoms, many compounds have been tried to be included in hair tonics and remedy. This study considers if there are any connection between the zinc known as essential trace elements and the hair growth. through mouse experiments (C57BL/6). The effect of zinc sulfate and zinc chloride on the hair growth of mouse model has been investigated by visual observation (in terms of hair growth score) and histological section analysis. The experiment was conducted over a total of 6 weeks after depilating the dorsum of the C57BL/6 mice hair. No treatment and tonic water groups were negative controls and 3% minoxidil a positive control. Zinc solutions were mixed by the final concentration of 20 ppm ZnSO4 and ZnCl2 with a tonic water. Comparison of hair growth in C57BL/6 mice dorsum was done after application of 2 mL zinc solutions. Experimental results were as follows: 3% minoxidil > ZnSO4 > tonic water > no treatment > ZnCl2 groups. Experimental skin tissue was cut in a transverse plane in order to analyze a morphological change in five weeks. As the result, the hair follicles and hair growth condition, hair papilla and hair root well-developed were observed. This result showed that the coated zinc solution on the skin has been absorbed in hair follicles, induces the production of the capillary, and increases in the melanin pigment cells related to enable the hair-growth by having influence on growth hormones around the hair follicle. If the experiment were carried out with a further more in-depth research such as the additional clinical trial, the potential of zinc would be considered as a therapeutic agent for alopecia.

P194 : The study of mechanism for hair growth using platelet rich plasma and combination of growth factors

( Joon Hyuk Suh ),( Moo Yeol Hyun ),( Yong Hee Lee ),( Kwang Ho Yoo ),( Hee Su Kim ),( Beom Joon Kim ),( Myeung Nam Kim ),( Chang Kwon Hong ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Platelet rich plasma(PRP) is widely used for hair growth treatment. However, the mechanisms of PRP and combination of growth factors are not fully understood yet. In an effort to develop new therapies to enhance hair growth, we screened PRP and combination of growth factors of it Objectives: We investigate the growth factors in PRP and compare the effect on hair growth between PRP and combination of growth factors Methods: We examined the effect of PRP and combination growth factors which mixed in different ratio on human dermal papilla cells and C57BL6/J mice using western blot analysis, real-time PCR and immunocytochemistry. Also, we did organ cultures of combining different growth factors for measuring hair length. Results: Combination of EGF and PDGF-AA (1:1 ratio) induced synergistic hair growth and anagen induction activity by regulation of hair growth-related signaling pathways and molecules in hDPCs and C57BL6/J mice model. Conclusion: Combination of EGF and PDGF-AA (1:1 ratio) can be further pharmaceutically developed as a therapeutic material against hair loss and alopecia in human.

Hair-Growth-Promoting Effect of Conditioned Medium of High Integrin α ₆ and Low CD 71 (α ₆ ^bri /CD71 ^dim ) Positive Keratinocyte Cells

Won, Chong Hyun,Jeong, Yun-Mi,Kang, Sangjin,Koo, Tae-Sung,Park, So-Hyun,Park, Ki-Young,Sung, Young-Kwan,Sung, Jong-Hyuk MDPI 2015 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.16 No.3

<P>Keratinocyte stem/progenitor cells (KSCs) reside in the bulge region of the hair follicles and may be involved in hair growth. Hair follicle dermal papilla cells (HFDPCs) and outer root sheath (ORS) cells were treated with conditioned medium (CM) of KSCs. Moreover, the effects of KSC-CM on hair growth were examined <I>ex vivo</I> and <I>in vivo</I>. A human growth factor chip array and RT-PCR were employed to identify enriched proteins in KSC-CM as compared with CM from keratinocytes. KSC-CM significantly increased the proliferation of HFDPCs and ORS cells, and increased the S-phase of the cell cycle in HFDPCs. KSC-CM led to the phosphorylation of ATK and ERK1/2 in both cell types. After subcutaneous injection of KSC-CM in C3H/HeN mice, a significant increase in hair growth and increased proliferation of hair matrix keratinocytes <I>ex vivo</I> was observed. We identified six proteins enriched in KSC-CM (amphiregulin, insulin-like growth factor binding protein-2, insulin-like growth factor binding protein-5, granulocyte macrophage-colony stimulating factor, Platelet-derived growth factor-AA, and vascular endothelial growth factor). A growth-factor cocktail that contains these six recombinant growth factors significantly increased the proliferation of HFDPCs and ORS cells and enhanced the hair growth of mouse models. These results collectively indicate that KSC-CM has the potential to increase hair growth via the proliferative capacity of HFDPCs and ORS cells.</P>

꽃송이버섯 추출물이 제모된 C57BL/6 마우스의 모발성장 및 억제에 미치는 영향

류은미,신현재,나명순 한국피부과학연구원 2010 대한피부미용학회지 Vol.8 No.4

Sparassis crispa is an edible and medicinal mushroom, which commonly called the cauliflower mushroom. S. crispa is known to be a good source of food and nutraceuticals due to their rich flavors and β-glucan contents as well as a good source of antiviral and anticancer compounds. However, there have been no report on the effect on hair growth stimulation and/or growth inhibition. C57BL/6 mice the most widely used lab mouse strain were used in this animal study due to their availability and the similar hair growth cycle to human hair. In this study, hair-removed five-year-old C57BL/6 mice were used to examine the effect of S. crispa extracts(70% ethanol) on hair growth stimulation and inhibition of the mice. The extracts and control compounds were topically treated once a day for 4 weeks. The hair growth was photographically and histologically examined during the total period of 4 weeks. Hair growth of the test group was a little inhibited compared to positive (3% minoxidil) and negative control groups (water and ethanol treated groups), respectively. The whole data showed little improvement of hair growth effect of S. crispa extracts but with no real statistical significance. These results suggest that S. crispa extract has little hair growth promoting activity and more works should be done to elucidate the inhibition mechanism. 본 연구는 최근 면역 및 항암활성 등 혈류 개선과 관련된 약리적 효과로 주목받고 있는 꽃송이버섯 추출물이 발모․양모 및 제모에 어떠한 영향을 미치는지 알아보고자 실험동물을 통하여 실험적 연구를 수행하였다. 실험은 실험군의 꽃송이버섯 추출물과 대조군의 무시료, 에탄올 70%, 버섯추출물, 미녹시딜 3%로 나누어 실험동물 C57BL/6 mouse(female)에 처리하였다. 실험시료에 따라 실험군과 대조군으로 나누어 총 5군을 실험에 이용하였다. 7주령 mouse의 등판을 제모한 뒤 4주(28일)동안 시료에 따른 양모효과를 육안적 관찰하였고, 경피 적출 후 조직학적인 검사를 수행하여 다음과 같은 결과를 얻었다. 1. 털의 성장정도를 알아보기 위한 mouse 외형의 육안적 관찰결과, 실험군인 꽃송이버섯 추출물이 대조군의 버섯추출물, 에탄올, 무시료, 미녹시딜 보다 모발의 성장이 억제됨이 관찰되었다. 2. 4주(28일)째 mouse를 경추 도살하여 얻은 피부조직의 모낭형태를 광학현미경으로 관찰한 결과, 실험군인 꽃송이버섯 추출물에서 대조군에 비해 모낭조직의 길이가 짧고 피부 위쪽에 위치하고 있었으며, 모낭내의 모근이 짧게 성장하여 피부의 표면으로 자라는 모낭 형태를 하고 있었다. 결론적으로 꽃송이버섯 추출물을 C57BL/6 mouse(female)의 등부위에 처리한 결과, 육안적 외형분석 및 조직학적 분석에서 실험군이 대조군보다 모발 성장이 억제되는 것으로 나타남에 따라 실험군인 꽃송이버섯 추출물은 모발 성장억제 효과가 있는 것으로 사료된다.

Effects of a gintonin-enriched fraction on hair growth: an in vitro and in vivo study

Lee, Na-Eun,Park, Sang-Deuk,Hwang, Hongik,Choi, Sun-Hye,Lee, Ra Mi,Nam, Sung Min,Choi, Jong Hee,Rhim, Hyewhon,Cho, Ik-Hyun,Kim, Hyoung-Chun,Hwang, Sung-Hee,Nah, Seung-Yeol The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.1

Background: Ginseng has been widely used as a health-promoting tonic. Gintonin present in ginseng acts as a lysophosphatidic acid (LPA) receptor ligand that activates six LPA receptor subtypes. The LPA6 subtype plays a key role in normal hair growth, and mutations in the LPA6 receptor impair normal human hair growth. Currently, human hair loss and alopecia are concerning issues that affect peoples' social and day-to-day lives. Objective: We investigated the in vitro and in vivo effects of a gintonin-enriched fraction (GEF) on mouse hair growth. Methods: Human hair follicle dermal papilla cells (HFDPCs) and six-week-old male C57BL/6 mice were used. The mice were divided into the four groups: control, 1% minoxidil, 0.75% GEF, and 1.5% GEF. The dorsal hair was removed to synchronize the telogen phase. Each group was treated topically, once a day, for 15 days. We analyzed hair growth activity and histological changes. Results: GEF induced transient [Ca<SUP>2+</SUP>]<SUB>i</SUB>, which stimulated HFDPC proliferation and caused 5-bromo-2'-deoxyuridine (BrdU) incorporation in a concentration-dependent manner. GEF-mediated HFDPC proliferation was blocked by the LPA receptor antagonist and Ca<SUP>2+</SUP> chelator. HFDPC treatment with GEF stimulated vascular endothelial growth factor release. Topical application of GEF and minoxidil promoted hair growth in a dose-dependent manner. Histological analysis showed that GEF and minoxidil increased the number of hair follicles and hair weight. Conclusion: Topical application of GEF promotes mouse hair growth through HFDPC proliferation. GEF could be one of the main components of ginseng that promote hair growth and could be used to treat human alopecia.