Selenium의 첨가가 육계 및 산란계의 생산성 및 축적에 미치는 효과

나재천,김상호,장병귀,김지혁,유동조,이덕수,이상진,이종찬,이원준,Na, J.C.,Kim, S.H.,Jang, B.G.,Kim, J.H.,Yu, D.J.,Lee, D.S.,Lee, S.J.,Lee, J.C.,Lee, W.J. 한국가금학회 2006 韓國家禽學會誌 Vol.33 No.3

셀레늄의 첨가가 육계 및 산란계의 생산성과 축적에 미치는 영향을 구명하기 위하여 2차례의 실험을 수행하였다. 실험 1은 무기태 selenium 0.12, 0.24 ppm과 유기태 selenium 0.12, 0.24 및 0.6 ppm의 첨가가 육계의 사료 섭취량, 증체량, 사료 요구율 및 닭고기 내 selenium 축적에 미치는 영향을 조사하였다. 시험 전 기간의 증체량은 무기태 0.24 ppm 첨가구보다 유기태 selenium 0.24와 0.60 ppm 첨가구에서 높았다(P<0.05). 그러나 사료 섭취량과 사료 요구율은 대조구, 무기태 및 유기태 selenium의 첨가 수준에 의한 차이는 없었다. 가슴과 다리근육의 selenium 함량은 대조구에 비하여 유기태와 무기태 selenium 첨가구가 높았으며(P<0.05), 유기태 selenium의 첨가 수준에 따른 차이는 있으나(P<0.05), 무기태 selenium의 첨가 수준에 따른 차이는 없었다. 실험 2에서는 산란계 사료에 무기태 selenium 0.06, 0.12 ppm과 유기태 selenium 0.06, 0.12 및 0.30 ppm의 첨가하여 급여시에 산란율, 사료 섭취량, 사료 요구율, 난질 및 계란 내 selenium 함량에 미치는 영향을 조사하였다. 사료 요구율은 무기태 0.06 ppm 첨가구가 대조구보다 유의적으로 개선되었으나(P<0.05), 산란율, 난중, 1일 산란량, 사료 섭취량은 대조구, 무기태 및 유기태 selenium의 첨가 수준에 따른 차이는 없었다. Haugh unit는 모든 처리구에서 차이가 없었으며, 난황색은 유기태 selenium 0.30 ppm 첨가구가 대조구를 포함한 다른 처리구에 비하여 높았다(P<0.05). 난각 강도는 유기태 selenium 0.06 ppm 첨가구가 높았다.(P<0.05). 그러나 난각 두께는 대조구를 포함한 모든 처리구에서 차이는 없었다. 계란 중의 selenium 함량은 대조구에 비하여 selenium을 첨가한 모든 처리구에서 높았다(P<0.05). 이외에도 사료내 selenium 첨가 수준에 비례하여 계란에서 selenium 축적량도 증가하였으며(P<0.05), 특히 유기태 selenium 이 무기태 selenium보다 효과적이었다. Two experiments were conducted to investigate the effect of dietary selenium sources on performance and selenium retention in broiler chickens and laying hens. In experiment 1, the effects of dietary selenium sources and levels on the weight gain, feed intake, feed conversion, and selenium retention of meat in broiler chickens were investigated. for each growth phase, the basal diet was supplemented with 0 (control), 0.12 and 0.24 ppm Se from sodium selenite (SS) and 0.12, 0.24 and 0.60 ppm Se from selenium yeast(SY). Weight gain was significantly increased(P<0.05) in supplemental 0.24 and 0.60 ppm SY compared to the 0.24 ppm SS by diet during day 1 to 35, but feed intake and feed conversion were not affected by the source or the level of Se. Selenium concentrations of breast and leg muscle were significantly increased(P<0.05) in supplemental SS and SY compared to the control, and linearly increased(P<0.05) as dietary. Se level increased by SY, but there was no difference in supplemental 0.12 ppm SS compared to 0.24 ppm SS. In experiment 2, 12-week-experiment using Hy-Line laying hens(31 wk of age) was conducted to compare the effects of selenium sources and levels on egg production, egg weight, daily egg mass, feed intake, feed conversion, egg quality, and selenium retention of egg in laying hens. A corn-soybean meal basal diet was supplemented with 0 (control), 0.06 and 0.12 ppm Se from sodium selenite (SS) and 0.06, 0.12 and 0.30 ppm Se from selenium yeast(SY). Feed conversion was significantly improved(P<0.05) in supplemental 0.06 ppm SS compared to the control, but egg production, egg weight, daily egg mass, and feed intake were not affected by source and level of Se. Haugh unit was not affected by source or level of Se. Yolk color was significantly(P<0.05) higher in supplemental 0.3 ppm SY compared to the control and other supplement in week 12. Eggshell breaking strength was significantly(P<0.05) higher in supplemental 0.06 ppm SY(P<0.05). Thickness of eggshell was not affected by source or level of Se. Se concentrations of egg was significantly improved(P<0.05) in supplemental SS and SY compared to the control, and was significantly increased(P<0.05) as dietary Se level increased by SS and SY, especially SY more effective compared to the SS.

RAW 264.7 세포에서 셀레늄이 유기수은에 의해 유도된 apoptosis에 미치는 영향

권근상,고대하,염정호,윤욱희 大韓産業醫學會 2003 대한직업환경의학회지 Vol.15 No.3

목적: 이 연구는 수은의 세포 독성에 대하여 방어 효괄르 나타낸다고 알려진 셀레늄아 수은에 의해서 유도되어지는 apoptosis에 미치는 영향을 파악하고자 실시하였다. 방법: 수은의 세포독성을 알아보기 위하여 MTT를이용. 미토콘드리아 내의 dehydrogenase 활성을 측정하였다. 또한 Hoechat-33258과 PI를 이용한 핵 염색, TdT를 이용한 dUTP-FITC nick-end labeling(TUNEL) 방법 및 Flow cytometry를 이용하여 apoptosis를 관찰하였다. 결과: 유기수은은 농도 의존적으로 세포독성 효과를 나타냈으며, 유기수은과 셀레늄을 동시에 처리한 군은 유기수은에 의한 세포독성을 셀레늄의 농도 의존적으로 감소시켰으나 셀레늄을 전 처리 한 후에 단독으로 유기수은만을 처리한 군은 유기수은에 의한 세포독성을 감소시키지 못했다. DNA 유전분석과 Hochest 33258 및 PI 염색을 이용한 핵 형태 분석을 통해 DNA laddering과 핵응축 그리고 apoptotic body를 확인하여 유기수은에 의해서 apoptosis가 유도된다는 것을 확인할 수 있었다. 또한 annexin V-FITC를 이용한 세포막 지질 구조 변화에서도 유기수은을 처리한 군에서 PS에 대한 annexin V의 강한 결합에 따라 유기수은에 의해 유도되는 apoptosis를 확인 할 수있었다. 셀레늄이 유기수은에 의해 유도되는 apoptosis에 미치는 영향을 조사한 결과, 유기수은과 셀레늄을 동시에 처리한 군은 핵 형태 변화, DNA 분절과 세포막 지질구조에 있어서 대조군과 같은 양상을 보인 반면 셀레늄을 전 처리 한 후에 단독으로 유기수은만능 처리한 군과 유기수은을 전처리 한 후 셀레늄을 처리한 군은 수은에 의한 apoptosis를 감소시키지 못했다. 결론: 이상의 결과는 셀레늄이 유기수은과 반응하여 복합체를 형성함으로써 수은에 의한 세포막 단백질 sulfhydryl group의 결합에 의한 세포막 손상 및 미토콘드리아 내막에 존재하는 세포 내 활성산소 해독에 관여하는 여러 효소와의 결합 등으로 인한 산화적 스트레스 유발을 차단함으로써 유기수은에 의해 유도되는 세포독성 및 apoptosis에 방어 효과를 나타내는 것으로 사료된다. Objects: This study was performed to evaluate the protective effects of selenium against the methyl mercury chloride (MehgCl) induced cell apoptosis. Methods: The effect of selenium on the MeHgCl induced cell apoptosis was observed in mouse macrophage-derived RAW 264.7 cells, in vitro. The cells were cultured in Dulbecco's modified Eagle's medium (DMEM). Results: MeHgCl exerted a dose dependent cytotoxicity, as demonstrated by the MTT assay, an assay dependent, in part, on mitochondrial function. Concurrent exposure to selenium provided complete protective effects against the cytptpxicity induced by MeHgCl. Pretreatment with selenium increased the protective effects of subsquent administrations of selenium in conjunction with MeHgCl, but pretreatment of selenium along did not provide protection against MeHgCl when given alone. Selenium administered after exposure to MeHgCl did not repair the existing MeHgCl induced cytotoxicity. Furthermore, the apoptosis induced by MeHgCl was revealed by the DNA fragmentation, using the terminal deoxynucleotidyl transferase Biotin-dUTP nick end labeling (TUNEL) assay, alterations to the nuclear morphology, by nuclei stauining, and the plasma membrane lipid organizations, as shown by cell flow cytometry. The apoptosis induced by MeHgCl was administration of selenium in conjunction with MeHgCl. However, no inhibittion of the MeHgCl induced apoptosis was observed with selenium pretreatment prior to exposure to MeHgCl alone, or with the administration of selenium after exposure to MeHgCl. Conclusions: These results suggest that the coexistence of selenium and MeHgCl are essential for the protective effects of selenium against the MeHgCl-induced apoptosis, and the cytotoxicity, in RAW 264.7 cells, and may involve selenium-MeHgCl binding.

Selenium이 MPTP(1-methy-4-phenyl-1,2,3,6-tetrahydropyridine)에 의해 유도된 생쥐의 신경독성에 미치는 영향

김석환,이주연,김여정,강혜옥,이항우,최종원,Kim Seck-Hwan,Lee Joo-Yeon,Kim Yeo-Jeong,Kang Hye-Ok,Lee Hang-Woo,Choi Jong-Won 한국생명과학회 2006 생명과학회지 Vol.16 No.2

MPTP에 의해 유도된 Parkinsonism에 대한 selenium의 보호효과와 그 보호작용에 대한 항산화적 해독기전을 조사하기 위하여 MPTP 10mg/kg을 6일간 주사하고 selenium (25, 50, 100 ${\mu}g/kg$)을 10일간 경구 투여하였으며 처음 6일간은 selenium와 MPTP를 병용 투여하였다. 실험동물을 마지막으로 selenium을 투여하고 24시간 후에 치사시켜 일반적인 독성과 항산화 방어능과 관련된 지표성분과 monoamine oxidase와 같은 신경생화학적인 지표성분들을 뇌조직에서 측정하였으며 그 결과 다음과 같은 결론을 얻었다. 우선 MPTP를 투여함에 따라 운동능력이 저하되던 것이 selenium을 투여함에 따라 운동능력이 증가되었으며, 이러한 결과의 기전은 selelnium을 투여함으로써 MPTP를 $MPP^+$로 대사시키는 MAO-B의 활성을 억제하였으며 $MPP^+$에 의해 유도된 신경독성에 대한 selenium의 보호 효과는 selenium을 투여함으로써 활성산소 해독계인 SOD, catalase, glutathione peroxidase의 활성을 증가시키기 때문인 것으로 사료된다. This study is investigated the effect of selenium against neurotoxicity induced by MPTP(1-methy-4-phenyl-propion-oxypiperidine) in mice. In order to demonstrate neuroprotective activity of selenium, mice were administrated orally with selenium(25, 50, 100 ${\mu}g/kg$, once/day) for 10 days, and MPTP(10 mg/kg) was injected subcutaneously into the mice for 6 days from the beginning 1hr before selenium treatment. Test of rota road activity was inhibited by treatment with selenium in MPTP-induced neurotoxicity group when compared to MPTP treatment group in normal mice. Monoamine oxidase(MAO)-B activity and cerebral lipid peroxide content were significantly decreased in the treatment of selenium in MPTP-induced neurotoxicity group when compared to MPTP treatment group in normal mice and MAO-A was not affected. Activities of cerebral superoxide dismutase, catalase and glutathione peroxidase were significantly increased in the treatment of selenium in MPTP-induced neurotoxicity group when compared to MPTP treatment group in normal mice. These results suggest that selenium might be estimated the result from the cooperative action of its inhibitory effect on monoamine oxidase-B with that of the enhancement of antioxidant(SOD, catalase, GSH-Px) defence ability.

α-Tocopherol과 Selenium의 첨가 급여가 육계의 생산성 및 닭 다리육의 이화학적 특성에 미치는 영향

김영직(Young-Jik Kim) 韓國家禽學會 2011 韓國家禽學會誌 Vol.38 No.2

본 실험은 α-tocopherol(0, 50, 100, 200 IU/kg)과 selenium (0.3 ppm) 및 α-tocopherol 100 IU/㎏과 selenium 0.3 ppm을 혼합 급여한 육계의 생산성, 계육의 일반 성분, pH, TBARS, WHC, 전단력, 육색 및 지방산 조성에 미치는 영향을 알아보고자 수행하였다. 육계 360수를 공시하여 대조구와 α-tocopherol 50 IU/㎏ 급여구를 T1, α-tocopherol 100 IU/㎏ 급여구를 T2, α-tocopherol 200 IU/㎏ 급여구를 T3, selenium 0.3 ppm/㎏ 급여구를 T4, α-tocopherol 100 IU/kg과 selenium 0.3 ppm/㎏ 급여구를 T5 등 6개 처리구로 나누어 5주간 사양한 후 다리살 근육을 분석하였다. 육계의 증체량, 사료 섭취량 및 사료 요구율은 처리구간에 유의성이 없었다. 계육의 일반 성분중에 수분과 조단백질, 조회분은 처리구간에 유의성이 없었고, 조지방 함량은 대조구와 T1보다 처리구에서 높았다. pH, 보수성과전단력은모든처리구에서유의성은없었다. TBARS 는 대조구와 T1은 유의한 차이가 없으나, α-tocopherol과 selenium 급여구에서낮았으며, 특히혼합급여구인T5에서TBARS 값이 유의하게 낮았다. 육색중 CIE L<SUP>*</SUP>값과 b<SUP>*</SUP>값은 처리구간에 유의성이 인정되지 않았으나, CIE a<SUP>*</SUP>값은 T2, T3, T5에서 높았다(P<0.05). 지방산의 분석 결과, α-tocopherol의 급여로 palmitic acid는 감소하고, oleic acid, linileic acid는 증가하여 포화지방산함량은줄고, 불포화지방산함량이증가하는 결과로 나타났다(P<0.05). 이상의 결과를 종합적으로 고찰해 볼때 α-tocopherol과 selenium를 육계에 급여하면 육색과 지방의 산화를 억제하는 효과가 있음을 확인할 수 있어 계육의 저장성을 개선할 가능성이 있을 것으로 기대된다. The objective of this study was to evaluate the effects of different levels of α-tocopherol and selenium in broiler diets on performance and physicochemical properties of chicken thigh meat. A total of 360 broiler chicks divided into six groups were fed a basal diet (control) or basal diet supplemented with 50 IU α-tocopherol/㎏ (T1), 100 IU α-tocopherol/㎏ (T2), 200IU α-tocopherol/㎏ (T3), 0.3 ppm selenium/㎏ (T4), and 100 IU α-tocopherol/㎏ + 0.3 ppm selenium/㎏ (T5) for five weeks. Growth performance and proximate composition of chicken thigh meat were not influenced by all dietary treatments, except for crude fat, which was significantly lower in the treatment with α-tocopherol or selenium, or both compared to control. The dietary α-tocopherol and selenium supplementation had no significant effects on pH, CIE L<SUP>*</SUP> (lightness), and b<SUP>*</SUP> (yellowness) values but TBARS (thiobarbituric acid reactive substance) values were decreased (P<0.05) by the addition of α-tocopherol and selenium. CIE a<SUP>*</SUP> (redness) values increased significantly with added α-tocopherol and selenium relative to the control (P<0.05). Likewise, changes in fatty acid composition in chicken thigh meat with the dietary supplementations with α-tocopherol, selenium and their combination significantly increased (P<0.05) unsaturated fatty acid, whereas saturated fatty acid were decreased. It was concluded that the addition of 200 IU α-tocopherol (T3) and 100 IU of α-tocopherol plus 0.3 ppm selenium (T5) were most effectiveness in the improvements in the lipid oxidative stability of chicken meat when compared to the control.

Maternal selenium-supplementation at various stages of periconception period: influence on murine blastocyst morphology and implantation status

Mamon, Mark Anthony C.,Ramos, Gliceria B. Korean Society of Animal Sciences and Technology 2017 한국축산학회지 Vol.59 No.4

Background: Selenium is one of the trace minerals whose deficiency is known to lead to complications of female reproduction. The identified gaps in researches regarding selenium and pregnancy include optimizing the dosage of selenium supplementation, timing of supplementation, finding the best form and type of selenium, and selenium administration combined with other antioxidants. Hence, this study was conceptualized to address one of the identified gaps, that is, to find out the best timing of selenium administration around the time of pregnancy. Specifically, this study aimed to assess the effects of maternal Selenium-supplementation, administered at various stages of periconception period, on murine blastocyst morphology, percent occurrence of good quality blastocysts, and implantation status. Methods: ICR female mice were randomly assigned into the unsupplemented group (Group I) receiving basal diet without selenium, and treatment groups given with $3.0{\mu}g$ selenium-supplement per day during pregestation only (Group II), pregestation-throughout-gestation (Group III) and gestation only (Group IV). Both blastocyst morphology and implantation status were assessed. Results: The morphometric measurements of blastocysts appeared to be unaffected by selenium-supplementation at different stages of periconception. Selenium-supplementation at pregestation only (Group II) and gestation only (Group IV) produced higher percent occurrence of good quality blastocysts and lower percent pre-implantation loss than Group III. Among all the treatment groups, Group III (Selenium-supplementation during pregestation-to-gestation) yielded the lowest quality blastocysts and highest percent pre-implantation loss. Conclusion: Maternal selenium-supplementation during pregestation and gestation stages of the periconception period yielded a high percent occurrence of good quality blastocysts and pre-implantation success.

셀레늄 결핍식이를 먹인 쥐를 대상으로 유기셀레늄의 생체이용률에 대한 연구

정은영(Eun Young Jung) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.9

본 연구는 세리신을 이용해 만든 유기셀레늄의 생체이용률을 알아보고자 하였다. 그 결과를 요약하면 다음과 같다. 항산화지표인 환원형 글루타티온은 셀레늄 처치로 인해 증가되었는데 유기셀레늄에 의한 증가는 무기셀레늄에 비해 큰 경향이었으며, 또한 혈중 과산화 지질도 유기셀레늄이 무기셀레늄에 비해 낮은 값을 나타내어 유기셀레늄은 무기셀레늄에 비해 항산화력 향상에 더 기여하는 것으로 나타났다. 셀레늄 결핍식이로 인해 저하된 셀레늄 흡수율과 보유율은 셀레늄 처치로 증가되는데, 특히 유기셀레늄 처치로 인한 흡수율과 보유율이 높아 생체 내 이용률은 증대될 것으로 사료되며 이는 항산화력 향상에 영향을 미칠 것으로 사료된다. 혈청과 간의 셀레늄의 농도는 셀레늄 처치로 유의하게 증가되는데 셀레늄 형태 중 유기셀레늄에 의한 증가가 가장 컸으나 통계적으로 유의한 수준은 아니었다. 결론적으로 본 연구의 결과 무기질을 함유한 펩타이드는 무기질의 생체이용률을 증가시키는 것으로 나타났는데 세리신을 이용한 유기화 형태로 섭취될 경우 혈액과 장기의 침착뿐 아니라 흡수율과 보유율 증가에 관여하여 각 무기질의 효능을 증진시키는 것으로 나타났다. We examined the effects of selenium-binding peptide from sericin hydrolysates on the bioavailability of selenium-deficient rats. Three-week-old male rats were fed a selenium-deficient diet for 4 weeks while the normal control group was fed a normal diet. The selenium-deficient rats were divided into three groups: no treatment, organic selenium (OS), and inorganic selenium (IS). After selenium supplementation for 4 weeks, the level of serum glutathione reduced form in rats treated with organic selenium was significantly higher than that of inorganic selenium. Selenium retention rate also increased significantly in the organic selenium group compared to the inorganic selenium group [selenium deficient diet (DD)+OS 50.25% vs. DD+IS 17.04%, P<0.05]. In conclusion, binding of selenium to peptides from sericin hydrolysates seems to improve its bioavailability, and can hasten a cure for selenium deficiency in experimental rats.

Effect of Dietary Selenium on the Colon Carcinogenesis in Male ICR Mice

Cho, Min-Haeng,Kim, Jun-Hyeong,Hue, Jin-Joo,Kang, Bong-Su,Park, Hyun-Ji,Nam, Sang-Yoon,Yun, Young-Won,Kim, Jong-Soo,Jeong, Jae-Hwang,Lee, Beom-Jun The Korean Society of Food Hygiene and Safety 2010 한국식품위생안전성학회지 Vol.25 No.3

본 연구에서는 azoxymethane (AOM)과 dextran sodium sulfate (DSS)로 유도된 대장 발암과정에 대한 셀레늄의 방어 효과를 조사하였다. 셀레늄 결핍(0.02 ppm Se), 정상(0.1 ppm Se), 과다(0.5 ppm Se)사료를 12주간 식이로 급여하여 혈액검사와 대장암 발생의 초기단계인 aberrant crypt foci (ACF)수를 측정했으며, 암 발생율을 조사하였다. ICP-AES를 사용하여 간의 셀레늄 농도를 측정하였으며, 또한 셀레늄포함 항산화효소인 glutathione peroxidase (GPx) 활성을 알아보았다. 또한 TUNEL assay와 PCNA, $\beta$-catenin에 대한 면역조직 염색을 수행하였다. ACF 수 및 종양 발생률에 있어서, 셀레늄과다사료를 급여한 군이 정상셀레늄사료를 급여한 군보다 낮았으며, 셀레늄결핍사료를 급여한 군은 오히려 ACF 수 및 종양 발생률이 높았다. GPx 활성은 셀레늄의 섭취가 과다한 군에서 높게 나타났으며, 이 때, TUNEL에서 apoptotic positive cell이 증가하는 것을 확인했다. 또한 셀레늄의 섭취가 과다한 군에서 PCNA와 $\beta$-catenin의 발현이 감소됨을 볼 수 있었다. 본 마우스 모델실험에서 셀레늄은 여러 기전에 의해 대장암 발생을 억제할 수 있을 것으로 사료된다. Selenium is an essential micronutrient for normal body function and functions as an essential constituent of selenoproteins. This study was carried out to investigate effect of selenium on the formation of colonic aberrant crypt foci (ACF) and tumor formation in a mouse model. Five-week old ICR mice were acclimated for one week and fed different selenium diet (0.02, 0.1, and 0.5 ppm) for 12 weeks. Animals received three intraperitoneal injections of azoxymethane (10 mg/kg B.W. in saline for 3 weeks), followed by 2% dextran sodium sulfate in the drinking water for a week. There were four experimental groups, including a normal control group and three different selenium levels groups. After sacrifice, the total numbers of aberrant crypt (AC) and ACF were measured in the colonic mucosa after methylene blue staining. The number of tumors was noted for tumor incidence. Liver selenium concentration was measured using ICP-AES method. Gutathione peroxidase (GPx) activity was determined using a GPx assay kit in the liver and colon. TUNEL assay and proliferating cell nuclear antigen (PCNA) staining were performed to examine the cell apoptosis and cell proliferation, respectively. Immunohistochemistry of $\beta$-catenin was also performed on the mucous membrane tissue of colon. The activity of GPx in the liver and colon was decreased in the selenium-deficient diet group while it was increased in the selenium-overloaded diet group. Apoptotic positive cells were increased in the selenium-overloaded diet group but decreased in the selenium-deficient diet group. PCNA staining area was decreased in the selenium-overloaded diet group. In addition, the $\beta$-catenin protein level in the selenium-deficient diet group was increased but decreased in the selenium-overloaded diet group. These results indicate that dietary selenium might exert a modulating effect on colon cancer by inhibiting the development of ACF and colon tumor formation in this mouse model.

Tellurium이 흰쥐 신장에 미치는 영향과 Selenium의 보상효과에 대한 미세구조적 연구

최월봉,조승묵,정영길,최창도 대한해부학회 1996 Anatomy & Cell Biology Vol.29 No.2

키토산올리고당 기질-유기태 셀레늄(Se-OCHT)이 산란계 계란 품질에 미치는 영향

조향현 ( Hyang Hyun Cho ),김희경 ( Hee Kyong Kim ),고홍범 ( Hong Bum Koh ),김영호 ( Young Ho Kim ) 한국키틴키토산학회 2016 한국키틴키토산학회지 Vol.21 No.3

가금류 사료에는 옥수수를 포함한 곡물류에 무기태 광물질이 첨가되어 사용되어지고 있다. 하지만, 원료 상승 및 경제적 여건과 배설로 인한 토양 및 수질오염, 독성 등으로 양계산업의 저해 요인이 되고 있어 이를 해결 할 방안이 필요하다. 본 실험에서는 키토산올리고당 기질 유기태 셀레늄을 일반 산란계 사료에 섭이시킨 후, 계란 무게, 가식부 무게, 난각 두께, 난황 착색도 등의 계란 품질 및 혈구수 변화 등을 각각 비교 · 평가하였다. 사양 시험은 Hy-line 산란계(20주령)을 대상으로 일반 산란계 사료에 무기태 또는 유기태 셀레늄을 일반 산란계 사료 셀레늄 함량 기준으로 농도별(1, 2, 4배)로 혼합하여 섭이시켜 평가 지표에 미치는 영향을 각각 비교하여 보았다. 본 실험 결과 유기태 셀레늄은 일반 사료를 섭이시킨 정상군, 무기태 셀레늄을 섭이시킨 군과 비교하여 가식부(난황+난백) 건조중량, 계란 난각 두께, 적혈구, 백혈구 수치를 증가시키는 것을 확인하였다. 하지만, 계란 총무게, 가식부 생중량(비건조중량), 난황 착색도 등은 정상군과 무기태 셀레늄과 비교하여 차이가 없었다. 결론적으로, 키토산올리고당 기질 유기태 셀레늄은 일반 사료를 섭이시킨 정상군, 무기태 군과 비교하여 계란의 품질 및 산란계 안전성 등에서 동등한 효과를 가지거나 효능을 증가시켜 축산 농가의 애로 사항을 해결할 수 있을 것으로 In this study, we investigated whether it is possible to substitute the inorganic selenium of chitosan oligosaccharide substrate organic selenium. We investigated the quality characteristics of egg produced from laying hens with non- supplemented diets or diets supplemented with organic selenium on chitosanoligosaccharide. Organic or non-organic selenium were mixed normal laying hen feedstuff. Laying hens were fed diet for 45 days containing powdered chitosan oligosaccharide-selenium, inorganic selenium or normal feedstuff. During the experimental period, organic selenium group increased the edible weight (dryness condition), egg shell thickness, white blood cell and red blood cell compared to inorganic selenium(p<0.05). And, egg weight, edible weight (not dryness condition), egg colors (yolk) and lymphocyte were no significant difference. But, each effectiveness are different from organic-selenium concentration to feeding period. It was investigated that organic selenium had equal or more effects compared with inorganic selenium or normal diet. These results suggest that organic selenium could be used as substitute for inorganic selenium as well as the supplement of laying hens diets to produce functional eggs.

Effect of Dietary Selenium on the Colon Carcinogenesis in Male ICR Mice

Min Haeng Cho,Jun-hyeong Kim,Jin-Joo Hue,Bong Su Kang,Hyunji Park,남상윤,윤영원,김종수,이범준,정재황 한국식품위생안전성학회 2010 한국식품위생안전성학회지 Vol.25 No.3

Selenium is an essential micronutrient for normal body function and functions as an essential constituent of selenoproteins. This study was carried out to investigate effect of selenium on the formation of colonic aberrant crypt foci (ACF) and tumor formation in a mouse model. Five-week old ICR mice were acclimated for one week and fed different selenium diet (0.02, 0.1, and 0.5 ppm) for 12 weeks. Animals received three intraperitoneal injections of azoxymethane (10 mg/kg B.W. in saline for 3 weeks), followed by 2% dextran sodium sulfate in the drinking water for a week. There were four experimental groups, including a normal control group and three different selenium levels groups. After sacrifice, the total numbers of aberrant crypt (AC) and ACF were measured in the colonic mucosa after methylene blue staining. The number of tumors was noted for tumor incidence. Liver selenium concentration was measured using ICP-AES method. Gutathione peroxidase (GPx) activity was determined using a GPx assay kit in the liver and colon. TUNEL assay and proliferating cell nuclear antigen (PCNA) staining were performed to examine the cell apoptosis and cell proliferation, respectively. Immunohistochemistry of β-catenin was also performed on the mucous membrane tissue of colon. The activity of GPx in the liver and colon was decreased in the selenium-deficient diet group while it was increased in the selenium-overloaded diet group. Apoptotic positive cells were increased in the selenium-overloaded diet group but decreased in the selenium-deficient diet group. PCNA staining area was decreased in the selenium-overloaded diet group. In addition, the β-catenin protein level in the seleniumdeficient diet group was increased but decreased in the selenium-overloaded diet group. These results indicate that dietary selenium might exert a modulating effect on colon cancer by inhibiting the development of ACF and colon tumor formation in this mouse model.