효소가수분해 도계부산물의 급여가 육계의 생산성 및 혈액 생화학적 특성에 미치는 영향

곽민근(Min-Geun Gwak),박혜성(Hye-Sung Park),김봉기(Bong-Ki Kim),박희복(Hee-Bok Park),김지혁(Ji-Hyuk Kim) 한국가금학회 2021 韓國家禽學會誌 Vol.48 No.3

본 연구는 단백질원으로서 가수분해 도계부산물(Enzymehydrolyzed poultry by-product meal)이 도계부산물(Poultry by-product meal) 및 대두박(Soybean meal)과 비교하여 육계의 생산성 등에 미치는 효과를 조사하고자 수행하였다. 실험에 사용된 가수분해 도계부산물은 SDS-PAGE와 MALDITOF 분석을 통해 가수분해가 잘 이루어져 저분자화 된 것을 확인하였다. 육계 300수를 이용해 1일령∼35일령까지 3처리 5반복, 반복당 20수씩 배치하여 급여 실험을 한 결과, 가수분해 도계부산물(EHPBM) 처리구는 종료 시 평균체중 1,853±125.60 g으로 도계부산물(PBM) 처리구 1,723±76.81 g , 대두박(SBM) 처리구 1,545±62.31 g 보다 유의적으로 높았다(P<0.05). 전 기간 사료요구율은 가수분해 도계부산물(EHPBM) 처리구에서 1.74±0.064로 도계부산물(PBM) 처리구 1.67±0.060, 대두박(SBM) 처리구 1.65±0.053보다는 높게 나타났다(P<0.05). 이는 도계부산물(PBM) 및 가수분해 도계 부산물(EHPBM) 처리구에서 대두박(SBM) 처리구에 비해 유의적으로 증가한 사료섭취량에 기인하는 것으로 보이며, 결과적으로 생산성 향상에 긍정적인 효과를 미친 것으로 판단된다. 각종 장기 무게에서는 대부분 유의성 있는 차이를 보이지는 않았고, F낭 무게에서는 유의차가 나타났으나 차이는 크지 않아 단백질 공급원이 각 처리구의 면역력에 명확한 영향을 주었다고 판단하기는 어렵다. 혈청 생화학 특성에서는 AST와 ALP가 가수분해 도계부산물(EHPBM) 처리구와 도계부산물(PBM) 처리구에서 유의적으로 높게 나타났다. 이는 이들 처리구의 증가한 사료섭취량과 급격한 체중 증가가 간에 영향을 미쳤기 때문인 것으로 추측된다. 결론적으로 가수분해 도계부산물은 육계의 생산성 향상에 효과적이며, 관행적인 단백질 원료들의 대체제로서 활용할 수 있을 것으로 사료된다. The purpose of this study was to investigate whether enzyme-hydrolyzed poultry by-product meal (EHPBM) is more effective as a protein source than poultry by-product meal (PBM) and soybean meal (SBM) for broiler chickens. A group of 300 one-day-old broiler chicks was randomly allocated to three treatments with five replicates (20 birds/replicate) for five weeks. The treatments consisted of basal diets containing 1) SBM, 2) PBM, and 3) EHPBM. The EHPBM-fed group (1,853 g±125.60) showed the highest final body weight (P<0.05) when compared to the PBM-fed group (1,723 g±76.81) and SBM-fed group (1,545 g±62.31). The feed conversion ratio of the EHPBM treatment group (1.740±0.104) was significantly higher (P<0.05) than those of the SBM (1.653±0.056) and PBM groups (1.674±0.072). It can be speculated that the increased feed intake in the EHPBM group led to higher body weight gain and FCR. There was no significant effect of treatments on internal organ weight except for the bursa of Fabricius. Blood biochemical characteristic analysis showed that aspartate aminotransferase and alkaline phosphatase levels were higher in the EHPBM and PBM groups (P<0.05), probably due to the strained liver caused by the rapid growth of birds. In conclusion, EHPBM may partly replace conventional dietary protein sources such as soybean meal or poultry by-product meal and can be used to improve the productivity of broilers.

Poultry By-Product Meal as a Potential Protein Source in Broiler Diets with Exogenous Protease Supplementation

Heshani Amalka Vithana,Shemil Priyan Macelline,Shan Randima Nawarathna,Dinesh Darshaka Jayasena,유명환,서은수,Mangala Amarsinghe,Maleeka Nadeemale Nambapana,Jin Ho Cho,허정민 한국가금학회 2023 韓國家禽學會誌 Vol.50 No.1

The objective of this study was to investigate the effect of exogenous protease supplementation in dietsformulated with poultry by-product meal on growth performance, small intestine magnitudes, and meat physiochemicalcharacters in broiler chickens from 21 to 35 days post-hatch. A total of 120, one-day-old "Arbor Acres Plus" broiler chickens(male: female - 50:50) were allocated one of two dietary treatments to give six replicates and ten birds per cage. Two dietarytreatments included a control diet (CON) and a diet supplemented with protease (CON+Pro). At day 35, body weight andfeed intakes were measured to calculate the feed conversion ratio for the entire experiment period. Two birds from each penwere euthanized to measure the relative lengths and relative weights of three small intestine sections and meat samples werecollected for physiochemical characteristic analyses at 35 days post-hatch. Exogenous protease supplementation did notinfluence (P>0.05) growth performance but showed a tendency to improve FCR (P=0.082). Protease supplementation showed a tendency to reduce proximal small intestine length (P=0.091). Broilers offered dietary treatments influenced minced meatcolor where protease supplementation resulted in lower CIE b* (P<0.001) colorimetric value for yellowness and showed a significant trend (P=0.059) on reducing meat redness CIE a*. In conclusion, the addition of exogenous protease to a broilerdiet formulated with poultry by-product meal did not affect the growth performance, small intestine magnitudes, and meatphysiochemical parameters (except CIE b*) in broiler chickens.

Effects of Replacement of Fish Meal with Poultry By-product Meals on Apparent Digestibility, Body Composition and Protein Efficiency Ratio in a Practical Diets for Rainbow Trout, Onchorynchus mykiss

Erturk, M.Mustafa,Sevgili, Huseyin Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.9

This study examined the replacement of the fish meal (FM) with commercial poultry by-product meal (PBM) in practical diets for rainbow trout (Onchorynchus mykiss, Walbaum). Five isocaloric and isonitrogenous diets containing levels 0 (control), 10, 20, 30 and 40% of PBM as a replacement for FM were fed to three replicate groups of rainbow trout with a initial weight $34.50{\pm}0.43g$ (mean${\pm}$SE). Protein efficiency ratio (PER) of diets containing PBM up to 20% were similar to the control while significantly lower values were obtained from the groups receiving higher levels of PBM (p<0.05). Apparent protein digestibility coefficients (ADCs) were significantly lower than that of the control group when PBM was included at level of 20% or more. Similarly, significantly lower values were observed with diets containing 30 and 40% PBM in terms of dry matter, organic matter, ether extract, ash and energy digestibility (p<0.05). Dietary treatments did not significantly affect the body composition of the fish in terms of dry matter, crude protein, ether extract and ash. In conclusion, PBM in a proportion of 20% may replace about 40 % of FM in rainbow trout diet without significant impairment grow.

On-farm evaluation of dietary animal and plant proteins to replace fishmeal in subadult olive flounder Paralichthys olivaceus

Wonsuk Choi,ALI HAMIDOGHLI,배진호,Seonghun Won,최윤희,김강웅,이봉주,허상우,한현섭,배승철 한국수산과학회 2020 Fisheries and Aquatic Sciences Vol.23 No.3

Background: High demand and low supply of fishmeal due to overexploitation of fisheries resources have resulted in a dramatic increase in the price of this ingredient. Olive flounder (Paralichthys olivaceus) commercial feed contains approximately 60% fishmeal and limited success has been achieved in identifying sustainable alternative protein sources for this species. Methods: An on-farm feeding trial was conducted to compare a basal diet containing 65% as the control (CONT) with two experimental diets replacing 10% of fishmeal by animal protein (AP10) or 20% of fishmeal by animal and plant protein (APP20). Sub-adult olive flounder averaging 327 ± 9.3 g (mean±SD) were fed one of the three diets in triplicate groups for 16 weeks. Results: Weight gain, specific growth rate, feed efficiency, protein efficiency ratio, and survival were not significantly different among fish fed all the experimental diets (P > 0.05). Also, non-specific immune responses (superoxide dismutase and lysozyme activity), serum biochemical parameters, and intestinal villi length were not significantly different among fish fed all the experimental diets (P > 0.05). Conclusions: Therefore, based on growth performance, non-specific immune responses, serum biochemical parameters, and intestinal histology, dietary animal and plant protein mixtures could replace up to 20% of fishmeal in the diet of sub-adult olive flounder.

On-farm evaluation of dietary animal and plant proteins to replace fishmeal in sub-adult olive flounder Paralichthys olivaceus

Choi, Wonsuk,Hamidoghli, Ali,Bae, Jinho,Won, Seonghun,Choi, Youn Hee,Kim, Kang-Woong,Lee, Bong-Joo,Hur, Sang-Woo,Han, Hyonsob,Bai, Sungchul C. The Korean Society of Fisheries and Aquatic Scienc 2020 Fisheries and Aquatic Sciences Vol.23 No.8

Background: High demand and low supply of fishmeal due to overexploitation of fisheries resources have resulted in a dramatic increase in the price of this ingredient. Olive flounder (Paralichthys olivaceus) commercial feed contains approximately 60% fishmeal and limited success has been achieved in identifying sustainable alternative protein sources for this species. Methods: An on-farm feeding trial was conducted to compare a basal diet containing 65% as the control (CONT) with two experimental diets replacing 10% of fishmeal by animal protein (AP₁₀) or 20% of fishmeal by animal and plant protein (APP₂₀). Sub-adult olive flounder averaging 327 ± 9.3 g (mean±SD) were fed one of the three diets in triplicate groups for 16 weeks. Results: Weight gain, specific growth rate, feed efficiency, protein efficiency ratio, and survival were not significantly different among fish fed all the experimental diets (P > 0.05). Also, non-specific immune responses (superoxide dismutase and lysozyme activity), serum biochemical parameters, and intestinal villi length were not significantly different among fish fed all the experimental diets (P > 0.05). Conclusions: Therefore, based on growth performance, non-specific immune responses, serum biochemical parameters, and intestinal histology, dietary animal and plant protein mixtures could replace up to 20% of fishmeal in the diet of sub-adult olive flounder.

효소가수분해 도계부산물의 급이가 육계의 생산성 및 혈액 특성에 미치는 영향

곽민근,김예림,박혜성,김계웅,김학연,김봉기,박희복,김지혁 한국가금학회 2019 한국가금학회 정기총회 및 학술발표회 Vol.2019 No.6