종양세포(腫瘍細胞)의 염색체(染色體)에 대한 오크라톡신 A의 독성(毒性)에 관한 연구(硏究)

윤화중,노민희,김강련,Yoon, Wha-jung,Roh, Min-hee,Kim, Kang-ryun 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.2

This study was performed to investigate the toxicity of ochratoxin A (OA) to the chromosomes of $K_{562}$ tumor cell-line in vitro. The results of this experiment were as follows: 1) Chromosomes of $K_{562}$tumor cell-line resulted in pseudotriploidy on the control group. Chromosomes of $K_{562}$ tumor cell-line treated with OA resulted in heteroploidy compared with the control group. The mean number of chromosomes in the karyotype of the control group (60) were 7 in the A group, 5 in the B group, 20 in the C+X group, 7 in the D group, 9 in the E group, 6 in the F group, and 6 in the G+Y group respectively. The number of chromosomes were increased as follows: Treating with $0.7{\mu}M$ OA, the number of chromosomes were increased one in E and F group, two in G+Y group compared with control group. In treated with $1.5{\mu}M$ OA, the increasing number of chromosome was one in E and F group. In treated with $3{\mu}M$ OA, E and F group was increased one and G+Y group were increased two chromosomes compared with control group. But in treated with $6{\mu}M$ OA, the number of chromosome in G+Y group was decreased one. 2) $K_{562}$ tumor cell line treated with OA showed Philadelphia-Chromosome in the long arm of the G group karyotype chromosome. The rate of chromosome aberration in $K_{562}$ tumor cell-line treated with OA was 77% in $0.7{\mu}M$ OA group, 71% in $1.5{\mu}M$ OA group, 82% in $3{\mu}M$ OA group and 94% in $6{\mu}M$ OA group respectively. The rate of chromosome aberration of $K_{562}$ tumor cell-line treated with OA was high in the high dose level of OA, and chromosome aberration of $K_{562}$ tumor cell-line treated with OA showed deletion, minute, dicentric-chromosome and translocation in the long arm of the C-group karyotype. As a result of this study, the toxicity of OA showed deletion, minute, dicentric-chromosome and translocation in the long arm of the C-group karyotype, and then, the toxicity of OA resulted in the damage to RNA and protein synthesis in $K_{562}$ tumor cell-line, and the C-group karyotype of $K_{562}$ tumor cell-line was target of the toxicity of OA.

와송(瓦松)이 만성 골수성 백혈병 세포주(K562)에서 세포사멸에 미치는 영향

윤경수,김영철,이장훈,우홍정,Yun, Kyoung-Su,Kim, Young-Chul,Lee, Jang-Hoon,Woo, Hong-Jung 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.1

Objectives : This study was performed to determine if Orostschys japonicus A. Berger has protective effects against CML in K562 cell lines. Materials and Methods : MTT assay, cell proliferation assay, Reverse transcription-polymerase reaction chain, RT-PCR, DNA fragmentation assay, Quantitative PCR were studied. Results : Orostschys japonicus A. Berger had no effects on Bax gene in K562 cell lines, but decreased Bcl-2 gene, and increased the Caspases-3 gene. This is indicate of induced apoptosis in K562 cell lines by Orostschys japonicus A. Berger. Conclusion : These results suggest that Orostschys japonicus A. Berger has effects on apatosis in K562 cell lines.

Combined Effects of Gamma-irradiation and Hyperthermia on the Human Cell Lines for Various Temperatures and Time Sequences

고경환(Kyung Hwon Koh),조철구 (Chul Koo Cho),박우윤 (Woo Yoon Park),류성렬 (Seong Yul Yoo),윤형근 (Hyong Geun Yun),심재원 (Jae Won Shim),이미정 (Mi Jung Lee). 대한방사선종양학회 1993 Radiation Oncology Journal Vol.11 No.1

원자력 병원 치료방사선과에서 사용 중인 극초단파 온열치료장치에 의한 온열치료시 온열치료의 적정화로 암 치료기술 향상을 도모함을 최종 목표로 하여 인체 암세포주에서 방사선과 온열에 의한 세포생존곡선의 기본적인 특징과 방사선과의 병용에 따른 치료효과의 증강을 파악하여 임상적 응용의 기초적인 근거를 확립하고자 하였다. 본 실험에서는 2개의 세포주, 즉 위 선암 세포주와 만성 골수성 백혈병 세포주를 사용하였다. 위암은 한국인에서 가장 발생빈도가 높고, 국소적으로 진행된 경우, 주위 장기의 한계선량으로 인하여 방사선 단독으로는 적용의 한계가 있어 온열치료와의 병용이 요구되며, 백혈병 세포는 방사선에 민감한 대표적인 세포의 하나로 온열치료와의 병용시 발생할 수 있는 미묘한 차이를 보다 분명히 관찰할 수 있을 것으로 기대하여 본 실험에 사용하였다. 감마선과 온열치료를 겸한 경우 두가지 방법을 동시에 시행한 경우, 치료효과가 가장 높았고 온열을 감마선 치료 후에 시행한 경우가 감마선 치료 전에 시행한 경우보다 약간 높았으나, 그 차이는 크지 않았고, 6시간 이상의 간격에서는 같았다. 온열치료의 온도 및 치료시간과 치료효과는 온도가 높을수록 치료시간이 길수록 치료효과가 높았고, 43℃에서는 온열내성을 시간경과에 따라 볼 수 있었다. 같은 온도로 30분간 온열치료시 온열치료효과 증진비는 섭씨 43도, 44도, 45의 경우, D 0.01을 기준으로 2.5±0.08, 3.75±0.18, 5.0±0.15였다. 본 연구는 기초 생물학적 실험이나, 이 결과는 임상 암치료에 직접 이용되는 것이며 또한 치료 수행에 반드시 필요한 과정이다. 따라서, 치료방법의 결정, 성적분석, 치료 부작용의 예측 및 대책 확립 등에 적용되고 온열치료의 특성 제시에 활용될 수 있다. We tried to establish the theoretical basis of clinical use of combined modality of hyperthermia and radiation therapy. For this purpose, we made an in vitro experiment in order to get the synergistic and/or additive effects on the cell killing of hyperthermia combined with radiation therapy by using the microwave-hyperthemia machine already installed at our department. In our experiment, we use two human cell lines: MKN-45 (Adenocarcinoma of stomach) and K-562 (leukemia cell lines). In cases of comblined treatments of hyperthermia and gamma-irradiation, the therapeutic effect was the highest in the simultaneous trial. Hyperthermia after gamma irradiation showed slightly higher therapeutic effect than that before irradiation without significant difference, but it's effect was the same in the interval of 6 hours between hyperthermia and irradiation. The higher temperature and the longer treatment time were applied, the higher therapeutic effects were observed. Wecould ovserve the thermoresistance by time elapse at 43℃, When hyperthermia was done for 30 minutes at the same temperature, thermal enhancement ratio (TER) at D001 (dose required surviving fraction of 0.01) were 2.5±0.08, 3.75±0.18, 5.0±0.15 at 43℃, 44℃, and 45℃ respectively in K-562 leukemia cell lines. Our experimental data showed that more cell killing effect can be obtained in the leukemia cell lines, although they usually are known to be radiosensitive, when treated with combined hyperthermia and radiation therapy. Furthermore, our data show that leukemia cell lines may have various intrinsic radiosensitivity, especially in bitro experiments. The magnitude of cell killing effect, however, will be less than that of MKN-45.

Ureaplasma urealyticum에 감염된 K-562 세포의 세포핵학적 특성

김광혁,유경식,박인달,장명웅 고신대학교(의대) 고신대학교 의과대학 학술지 1990 고신대학교 의과대학 학술지 Vol.6 No.2

When cultured human chronic myelogenous leukemia cell line(K-562) were exposed to Ureaplasma urealyticum, the cell showed chromosomal aberrations markedly. The K-562 cells were cultivated with U.urealyticum in RPMI 1640 without supplemental urea for 10 days. The cells for karyotyping were propagated in RPMI 1640 containing 10% fetal bovine surum. Chromosomal aberrations included chromosomal break, chromatid break, chromatid gap, chromosome exchange, chromatid exchange, and ring chromosome.

Ureaplasma urealyticum에 감염된 K-562 세포의 세포핵학적 특성

김광혁,유경식,박인달,장명웅 高神大學 醫學部 1990 高神大學醫學部 論文集 Vol.6 No.2

Inhibitory Effect of Curcumin on WT1 Gene Expression in Patient Leukemic Cells

Songyot Anuchapreeda,Pornngarm Limtrakul,Pattra Thanarattanakorn,Somjai Sittipreechacharn,Prasit Chanarat 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.1

Leukemias are common worldwide. Wilms’tumor1 (WT1) protein is highly expressed in leukemic blast cells of myeloid and lymphoid origin. Thus, WT1 mRNA serves as a tumor marker for leukemias detection and monitoring disease progression. Curcumin is well known for its anticancer property. The objective of this study was to investigate the effect of curcumin on WT1 gene expression in patient leukemic cells. The leukemic cells were collected from 70 childhood leukemia patients admitted at Maharaj Nakorn Chiang Mai Hospital, Chiang Mai, Thailand, in the period July 2003 to February 2005. There were 58 cases of acute lymphoblastic leukemia (ALL), 10 cases of acute myeloblastic leukemia (AML), and 2 cases of chronic myelocytic leukemia (CML). There were 41 males and 29 females ranging from 1 to 15 years old. Leukemic cells were cultured in the presence or absence of 10 mM curcumin for 48 h. WT1 mRNA levels were determined by RT-PCR. The result showed that curcumin reduced WT1 gene expression in the cells from 35 patients (50%). It affected the WT1 gene expression in 4 of 8 relapsed cases (50%), 12 of 24 cases of drug maintenance (50%), 7 of 16 cases of completed treatment (44%), and 12 of 22 cases of new patients (54%). The basal expression levels of WT1 gene in leukemic patient cells as compared to that of K562 cells were classified as low level (1-20%) in 6 of 20 cases (30%), medium level (21-60%) in 12 of 21 cases (57%), and high level (61-100%) in 17 of 23 cases (74%). In summary, curcumin decreased WT1 mRNA in patient leukemic cells. Thus, curcumin treatment may provide a lead for clinical treatment in leukemic patients in the future.

Inhibitory Effect of Curcumin on WT1 Gene Expression in Patient Leukemic Cells

Anuchapreeda, Songyot,Limtrakul, Pornngarm,Thanarattanakorn, Pattra,Sittipreechacharn, Somjai,Chanarat, Prasit The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.1

Leukemias are common worldwide. Wilms'tumor1 (WT1) protein is highly expressed in leukemic blast cells of myeloid and lymphoid origin. Thus, WT1 mRNA serves as a tumor marker for leukemias detection and monitoring disease progression. Curcumin is well known for its anticancer property. The objective of this study was to investigate the effect of curcumin on WT1 gene expression in patient leukemic cells. The leukemic cells were collected from 70 childhood leukemia patients admitted at Maharaj Nakorn Chiang Mai Hospital, Chiang Mai, Thailand, in the period July 2003 to February 2005. There were 58 cases of acute lymphoblastic leukemia (ALL), 10 cases of acute myeloblastic leukemia (AML), and 2 cases of chronic myelocytic leukemia (CML). There were 41 males and 29 females ranging from 1 to 15 years old. Leukemic cells were cultured in the presence or absence of 10 mM curcumin for 48 h. WT1 mRNA levels were determined by RT-PCR. The result showed that curcumin reduced WT1 gene expression in the cells from 35 patients (50%). It affected the WT1 gene expression in 4 of 8 relapsed cases (50%), 12 of 24 cases of drug maintenance (50%), 7 of 16 cases of completed treatment (44%), and 12 of 22 cases of new patients (54%). The basal expression levels of WT1 gene in leukemic patient cells as compared to that of K562 cells were classified as low level (1-20%) in 6 of 20 cases (30%), medium level (21-60%) in 12 of 21 cases (57%), and high level (61-100%) in 17 of 23 cases (74%). In summary, curcumin decreased WT1 mRNA in patient leukemic cells. Thus, curcumin treatment may provide a lead for clinical treatment in leukemic patients in the future.

Design, Synthesis and Pharmacophoric Model Building of Novel Substituted Nicotinic Acid Hydrazones with Potential Antiproliferative Activity

Hatem A. Abdel-Aziz,Tarek Aboul-Fadl,Abdul-Rahman M. Al-Obaid,Mohamed Ghazzali,Abdullah Al-Dhfyan,Alessandro Contini 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.9

Novel 6-aryl-2-methylnicotinic acid hydrazides 4a-c and their corresponding hydrazones 5a-c and 6a-i were synthesized. X-ray single crystal diffraction of 6h confirmed the chemical structure of hydrazones 6a-i. Antiproliferative activity of the synthetic compounds was investigated against K562 leukemia cell lines. Variable cell growth inhibitory activities were obtained with IC50 range from 24.99 to 66.78 μM where the compound 6c exhibited the maximum activity. Structure activity relationship analysis has been performed and a common pharmacophore model for the synthesized derivatives has been obtained by using the pharmacophore elucidation module of the software MOE. The best model obtained is characterized by two projected locations of potential H-bond donors (F3 and F4) and two Aromatic annotations (F1 and F2).

<i>Hericium erinaceus</i> isolectins recognize mucin-type <i>O</i>-glycans as tumor-associated carbohydrate antigens on the surface of K562 human leukemia cells

Elsevier 2018 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.120 No.1

<P><B>Abstract</B></P> <P>In <I>Hericium erinaceus</I> mushroom fruiting body, two different lectin groups, HEL1 and HEL2, were identified by using peptide mass fingerprinting based on customized protein sequence databases derived from RNA-Seq data. The HEL2 group included four isoforms designated HEL2a–d. Codon-optimized genes encoding HEL1, HEL2a, and HEL2b were expressed in <I>Escherichia coli</I> to produce fully active soluble proteins designated rHEL1, rHEL2a, and rHEL2b. Interestingly, these lectins showed different molecular weights: approximately 15 kDa for rHEL1 and approximately 120 kDa for rHEL2a and rHEL2b under non-denaturing conditions. rHEL2a and rHEL2b exhibited agglutination activities, but rHEL1 did not show any agglutination activity toward animal erythrocytes. The hemagglutination activity of rHEL2 lectins was strongly inhibited by glycoproteins containing mucin-type <I>O</I>-glycans. Glycan array analysis and isothermal titration calorimetry revealed that rHEL2 isolectins interacted strongly with <I>O</I>-glycans harboring the core 1 <I>O</I>-glycan motif, Galβ(1,3)GalNAc. Moreover, the glycan binding specificities of rHEL2 isolectins were comparable to that of peanut agglutinin in their ability to recognize <I>O</I>-glycans attached to leukosialin as tumor-associated carbohydrate antigens on the surface of K562 human leukemia cells. These results indicate that rHEL2 isolectins could be used as a powerful tool for analyzing mucin-type <I>O</I>-glycans expressed on the surface of cancer cells.</P>

Propofol 농도에 따른 말초혈액 단핵세포의 세포독성 평가

송호경 ( Ho Kyung Song ),정대철 ( Dae Chul Jeong ) 대한마취과학회 2003 Korean Journal of Anesthesiology Vol.44 No.4