구기자 투여 간손상 흰쥐에서 GOT 및 GPT의 활성화 연구

김병원,노광수 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.3

구기자의 한방 약리효과를 보기 위하여 구기자 혼합 생체시료와 세포배양한 캘러스로부터 추출한 열수 추출물을 사염화탄소로 유발된 간장해 흰쥐 (SD계)에 하루 0.5 g/kg을 경구 투여한 결과, 대조군 (GOT 949, GPT 640 karmen unit)에 비하여 혼합 생체시료 (GOT 760.4, GPT 540 karmen unit) 와 세포배양 캘러스 (GOT 772.1, GPT 556.4 karmen unit) 처리구에서 거의 비슷한 혈청 중 GOT, GPT 활성의 상승을 억제하는 효과를 보였으며, 또한 비교약물 sylimarin 0.1 g/kg 투여 군에서도 대조군에 비하여 혼합 생체시료 (GOT 492.6, GPT 320.4 karmen unit)와 세포배양 캘러스 (GOT 492.6, GPT 320.4 karmen unit) 처리구에서 거의 비슷한 혈청 중 GOT, GPT 활성의 상승을 억제하는 효과를 보였다. 이 상의 결과를 통하여 볼 때 구기자 생체시료와 세포배양 캘러스는 간손상 흰쥐에 대한 간보호 효과가 동일하게 있는 것으로 판단된다. The present study was undertaken in order to investigate betaine production by tissue culture and its medicinal effect in Lycium chinense Mill. In order to investigate the protective effect of L. chinense on the hepatoxicity induced by CCl4, 0.5 g/kg water extract of the compound mixture (leaves, roots and shoots) of L. chinense and its callus were fed to rat (SD line) once a day. As a result, the activity of GOT and GPT in the the goup fed compound mixture (GOT 760.4 and GPT 540 Karmen unit) and callus (GOT 772.1 and GPT 556.4 Karmen unit) was decreased in the blood serums relative to the controlled rat group (GOT 949 and GPT 640 Karmen unit) and the same result was obtained in the group fed with 0.1 g/kg sylimarin (the activity of GOT and GPT was shown 492.6 and 320.4 Karmen unit respectively. These results strongly indicate that water extracts of the mixture and callus from L. chinense do have the same decreasing effect of GOT and GPT in the hepatotoxic rat induced by CCl4.

스트롱 흥미검사의 GOT-BIS 요인구조 재검토

유현실,이은경 한국진로교육학회 2010 진로교육연구 Vol.23 No.4

The purpose of this study is to examine whether the General Occupational Themes(GOT)-Basic Interest Scale(BIS) factor structure of Korean-version Strong Interest Inventory(SII) is the same in the Korean college student sample as that of the U.S. version published in the year of 1994. A confirmatory factor analysis was conducted with the data collected from 3,148 freshmen attending a 4-year university located in the Seoul metropolitan area. The result showed that the BIS factor structure did not fit the GOT codes of the 1994 U.S. version. After disconfirming the factor structure, a exploratory factor analysis was conducted on the same dataset and the result was found that some scales of BIS corresponded to different GOT codes presented in the 1994 U.S. version. These results showed that the factor structure of GOT and BIS in the group of Korean college students may not correspond to that of original American version, which implies the possibility of different classification pattern of vocational interests from that of the Western culture. This study also suggests considering the cultural differences when interpreting the GOT and BIS of the Strong Interest Inventory for Korean clients. 본 연구의 목적은 스트롱 흥미검사의 일반직업분류(General Occupational Themes: GOT)와 기본흥미척도(Basic Interest Scales: BIS)의 요인구조가 1994년판 미국 스트롱 흥미검사에서 제시하는 요인구조와 합치되는지를 한국 대학생 집단에서 검토하는 것이다. 이를 위하여 서울 소재의 한 4년제 대학교 신입생 3,148명을 대상으로 실시한 스트롱 흥미검사 자료에 대하여 확인적 요인분석을 실시하였다. 그 결과 한국 대학생 집단에서 GOT-BIS의 요인구조는 2001년판 한국 스트롱 흥미검사에서 제시하는 GOT-BIS 요인모형과 합치되지 않는 것으로 나타났다. 이에 따라, 다시 동일한 자료를 가지고 최대우도법에 따른 탐색적 요인분석을 실시하였다. 그 결과 BIS의 일부 척도들이 스트롱 흥미검사 GOT 구조와는 다른 코드에 속해 있는 것으로 나타났다. 이러한 결과들은 한국 대학생 집단에서는 스트롱 흥미검사의 GOT와 BIS의 요인구조가 미국판 스트롱 흥미검사의 요인구조와 일치하지 않음을 뜻한다. 따라서 한국 대학생 집단에서 BIS 상에서의 개별 직업에 대한 흥미를 유목화하는 방식이 서구 문화권의 유목화 방식과 다를 수 있으며 향후 한국 내담자를 대상으로 스트롱 흥미 검사를 해석할 때 GOT와 BIS 척도의 해석에서 문화적 차이점을 고려해야할 필요가 있음을 시사한다.

Glutamic-oxaloacetic transaminase 1 regulates adipocyte differentiation by altering nicotinamide adenine dinucleotide phosphate content

Yang Yang,Cheng Zhimin,Zhang Wanfeng,Hei Wei,Lu Chang,Cai Chunbo,Zhao Yan,Gao Pengfei,Guo Xiaohong,Cao Guoqing,Li Bugao 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.2

Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation. Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content. Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p< 0.01), whereas GOT1 overexpression significantly increased lipid accumulation (p<0.01). At the same time, GOT1 knockout significantly decreased the NADPH content and the expression of MDH1 and ME1 in the 3T3-L1 cells. Overexpression of GOT1 significantly increased the NADPH content and the expression of MDH1 and ME1, suggesting that GOT1 regulated adipocyte differentiation by altering the NADPH content. Conclusion: The results preliminarily revealed the effector mechanisms of GOT1 in regulating adipose differentiation. Thus, a theoretical basis is provided for improving the quality of pork and studies on diseases associated with lipid metabolism. Objective: This study was performed to examine whether the porcine glutamic-oxaloacetic transaminase 1 (GOT1) gene has important functions in regulating adipocyte differentiation.Methods: Porcine GOT1 knockout and overexpression vectors were constructed and transfected into the mouse adipogenic 3T3-L1 cells. Lipid droplets levels were measured after 8 days of differentiation. The mechanisms through which GOT1 participated in lipid deposition were examined by measuring the expression of malate dehydrogenase 1 (MDH1) and malic enzyme (ME1) and the cellular nicotinamide adenine dinucleotide phosphate (NADPH) content.Results: GOT1 knockout significantly decreased lipid deposition in the 3T3-L1 cells (p<0.01), whereas GOT1 overexpression significantly increased lipid accumulation (p<0.01). At the same time, GOT1 knockout significantly decreased the NADPH content and the expression of MDH1 and ME1 in the 3T3-L1 cells. Overexpression of GOT1 significantly increased the NADPH content and the expression of MDH1 and ME1, suggesting that GOT1 regulated adipocyte differentiation by altering the NADPH content.Conclusion: The results preliminarily revealed the effector mechanisms of GOT1 in regulating adipose differentiation. Thus, a theoretical basis is provided for improving the quality of pork and studies on diseases associated with lipid metabolism.

일부 폐결핵환자의 HBs Ag. 발현빈도와 HBs Ag. 음양성자 간의 GOT 치와 GPT 치의 비교 검토

김태전,김승곤 서울保健大學 1981 論文集 Vol.1 No.1

폐결핵환자 300명을 대상으로 HBs ag.와 GOT와 GPT치를 검사한 성적을 요약하면 다음과 같다. 1) 폐결핵환자에서의 HBs ag. 양성율은 20%로서 대조군의 4.1%보다 유의하게 높았다(p<0.05). 2)HBs ag. 양성자의 GOT(43.2±2.0unit), GPT(29.3±1.8unit)치는 음성자의 GOT(35.4±1.3unit), GPT(19.3±1.9unit)치보다 다소 높은 경향을 보였다. 따라서 폐결핵환자 치료시에는 완전 멸균된 주사침을 사용해야 함은 물론 항결핵제가 간에 미치는 부작용을 감안 HBs ag.에 대한 검사를 반드시 실시해야 할 것이며 HBs ag.의 음양성이 GOT치나 GPT치에 큰 영양을 주지는 못하는 것으로 생각된다. This study was done to see HBs antigen positive rate of patients, with pulmonary tuberculosis, and to compare GOT, GPT levels between HBs antigen positive and negative patients. The results obtained were summarized as following: 1) As HBs antigen positive rate was 20% in patients with pulmonary tuberculosis, it was significantly higher than HBs positive rate (4.1%) of control group. (P<0.05). 2) GOT, GPT levels of HBs antigen positive patients (GOT:41.3±2.0 unit, GPT:29.3±1.8 unit) had a slightly higher trend than those of HBs antigen negative ones. As the results of this study, when patients were medicated for pulmonary tuberculosis, needles completely sterilized should be used, also tests of HBs antigen had to be done in them, and it was thought that GOT, GPT levels were not largely influenced by occurrance of HBs antigen.

MOLECULAR GENETIC STUDIES OF KOREAN POPULATION : 2. GENIC VARIATIONS OF PGM AND GOT 2. PGM, GOT의 유전적 변이에 관하여

KIM, YUNG JIN,KIM, JONG SOON,LEE, CHUNG CHOO 충남대학교 기초과학연구소 1987 연구논문집 Vol.7 No.-

The blood samples obtained from 500 unrelated individuals and the liver samples from 200 induced abortuses were studied with respect to the PGM (PGM_1, PGM_2 and PGM_3) and the GOT (GOT-s and GOT-m) enzymes using starch gel electrophoresis methods. The calculated gene frequencies were: PGM_1^1, 0.787, PGM_1^2, 0.203 and PGM_1 rare allele, 0.010 in the liver samples, and PGM_1^1, 0.775, PGM_1^2, 0.215 and PGM_1 rare allele, 0.010 in hemolyasates. The PGM_2 locus was almost all fixed to the PGM_2^1 allele in both liver and hemolysate samples. However, at the PGM_3 locus the gene frequencies were: PGM_3^1, 0.832 and PGM_3^2, 0.168 in the liver sample. The GOT-s^1 and GOT-m^1 allele were occurred at frequency of 0.965 and 0.993, respectively at the GOT-s and the GOT-m locus in the liver samples. The gene frequencies were compared with those of other racial populations.

소아 뇌막염에서 뇌척수액의 LDH활성도, GOT치, Cholesterol치에 관한 연구

정우영,박병욱,황태규,이순용 인제대학교 1986 仁濟醫學 Vol.7 No.4

뇌막염에서 뇌척수액 내에 존재하는 각종 화학 성분 및 효소의 변화를 초래함은 잘 알려져 있다. 저자들은 이들 중 뇌척수액의 LDH 활성도, GOT치, Cholesterol치를 측정하여 이들의 진단적 가치와 치료 결과 판정에서의 유용성을 조사하였다. LDH activity, GOT and cholesterol levels in CSF were prospectively studied on 43 cases of patients with bacterial, aseptic and tuberculous types of meningitis and 11 cases of control group who were admitted to our department since June 1985. The results obtained were as follows: 1.The LDH activity in CSF was significantly increased in patients with meningitis in all types compared with those of control group respectively (P<0.005). Among these the cases of bacterial meningitis revealed most prominent increment. 2.The LDH activity in CSF suggested a worthy role to differentiate bacterial meningitis from aseptic meningitis and also from tuberculous meningitis but in a lesser degree. 3.The GOT and cholesterol levels in CSF were significantly increased respectively in patients with bacterial meningitis compared with those of control group (P<0.005, P<0.025). 4.The GOT and cholesterol levels in CSF suggested a limited value for differential diagnosis of meningitides compared with those of LDH activity. 5.For the evaluation of treatment and prognosis, the LDH activity in CSF was relatively significant but those of GOT and cholesterol levels were not helpful.

영양고갈-스트레스에 의해서 상승 발현하는 유전자(Got1과 Mat1)의 분석

박준석(Junseok Park),권영숙(Young-Sook Kwon),이은령(Eunryoung Lee),권기상(Kisang Kwon) 한국생명과학회 2014 생명과학회지 Vol.24 No.6

영양분의 제한공급은 인체에 큰 스트레스중의 하나로서, 분자수준의 유전자발현과 생리기능에 영향을 미친다. 영양고갈-스트레스 동안에 일어나는 세포반응을 이해하는 것은 다이어트를 실시할 때에 일어나는 부작용을 최소화할 수 있는 실마리를 제공해준다. Got1 유전자의 발현은 starvation 1시간부터 발현이 증가하다가 24시간에서 정상상태로 돌아왔다. Mat1 유전자의 발현은 starvation 1시간부터 24시간까지 지속적으로 발현이 증가하였다. Rat를 1-3일간 starvation에 의해서는 Got1 유전자의 발현은 큰 변화를 보이지 않았지만, Mat1 유전자의 발현은 cerebral cortex에서 현저하게 줄어드는 반면에 cerebellum과 lung에서는 1-2일간의 starvation에 의해서 유전자 발현이 증가하다가 3일째는 발현이 줄어들었다. Heart에서는 starvation에 의해서 유전자 발현이 관찰되지 않을 정도로 줄어들었다. 간헐 starvation (2일간 starvation 군과 2일간 starvation후 1일간 먹이를 공급한 것과 2일간 starvation + 1일간 먹이를 공급 + 2일간 starvation 군)으로 나누었다. Got1 유전자의 발현은 lung에서만 starvation 후 1일간 먹이를 공급한 군에서 아주 강한 발현을 보였다. liver의 경우는 2일 간 starvation 군과 2일간 starvation후 1일간 먹이를 공급한 군에서 발현이 약해진 후 2일간 starvation + 1일간 먹이를 공급 + 2일간 starvation 군에서 강한 발현을 보였다. Muscle에서는 starvation 시작과 동시에 발현이 현저히 감소 후 2일간 starvation후 1일간 먹이를 공급하면 정상상태로 돌아왔다. Mat1 유전자는 의미 있는 발현 변화가 없었다. Got1 유전자 발현은 ♂의 경우 NaCl 공급에 의해서 lung에서는 강한 발현을 보이고 thymus에서는 감소하였고 나머지에서는 뚜렷한 발현 변화가 관찰되지 않았다. ♀의 경우는 물 공급 보다가 NaCl 공급에 의해서 모두 약한 발현 양상을 보였다. Mat1 유전자의 발현은 ♂의 경우 NaCl 공급에 의해서 lung, kidney, muscle에서 약하지만 상승 발현이 관찰되었다. ♀의 경우는 NaCl 공급에 의해서 상승 발현 하는 것이 관찰되지 않았다. Restricted supply of nutrients may affect genes at the molecular level as well as physiological functions. Understanding the cellular responses during starvation is necessary for developing strategies to reduce damage caused by starvation stress. After 1 h of starvation, Got1 gene expression was increased but its expression returned to the normal state after 24 h. Mat1 gene expression continuously increased with starvation from 1 h until 24 hr. Rats starved for 1-3 days showed significant changes in expression of the Got1 and Mat1 genes, which were significantly reduced in the cerebral cortex and cerebellum. In the lung, gene expression was increased by starvation for 1-2 days but decreased on the third day. No differences were observed in gene expression in the heart. Strong Got1 lung gene expression was seen in the starvation group one day after restoration of the food supply. Muscle mass was significantly reduced at the start of starvation and remained the same after two days of starvation and one day after the food supply was restored. The Mat1 gene expression did not change. The Got1 was induced by NaCl and showed strong expression in the lung and the thymus, but the apparent decrease of the remaining changes were not observed in male rats. The Mat1 gene was not as sensitive as the Got1 gene to induction by NaCl. However, differences in gene induction by NaCl were evident between males and females, indicating that diet control of gene expression is associated with hormones.

강황 섭취 또는 저항 운동과의 복합 처치가 성인 남성의 간 기능 효소들에 미치는 영향

백승준 ( Seung-Joon Paek ),이상현 ( Sang-hyun Lee ),박정수 ( Jung Su Park ),김준수 ( Jun-soo Kim ),박동호 ( Dong-ho Park ) 한국운동생리학회(구 한국운동과학회) 2021 운동과학 Vol.30 No.2

PURPOSE: This study aimed to investigate the effect of turmeric intake alone or in combination with resistance training on liver enzymes in adult men who regularly consumed alcohol at least twice a week. METHODS: Twelve men (age: 36.0±5.26 years, weight: 99.9±17.9 kg, and height: 184.0±6.5 cm) were randomly assigned to the turmeric intake (2-3 g/day) alone (TI, n=6) group or the TI plus resistance training (60 minutes of RT 3 times a week) (TI+RT, n=6) group for 8 weeks. Blood was collected before and after 8 weeks of treatment. The analyzed variables included glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), γ-glutamyl transferase (γ-GTP), and GOT/GPT ratio. RESULTS: There was no significant interaction effect in all variables, but the main effect was found in all variables only in TI+RT group. The Cohen’s d effect size on the main effects of the TI+RT group on GOT (d=0.548), GPT (d=0.835), γ-GTP (d=0.623), and GOT/ GPT ratio (d=0.800) ranged from 0.548-0.835, respectively. However, body composition such as percent body fat (%, p>.05) and muscle mass (kg, p>.05) did not show the main effect on time points as well as the interaction effect (p>.05). CONCLUSIONS: Although the 8-week TI treatment did not show any statistically significant change, TI treatment showed a tendency to decrease in GOT, GPT, and γ-GTP, and the GOT/GPT ratio tends to increase. Therefore, there was a synergic effect of the TI plus RT treatment or the sum of the two treatments for 8 weeks.

四物湯의 抗癌劑 副作用 抑制에 關한 實驗的 硏究

안희덕 대한동의병리학회 1995 동의생리병리학회지 Vol.9 No.2

연구배경: 서의의 치료법인 抗癌劑 및 放射線療法이 癌細胞 뿐만 아니라 正堂細胞에까지 毒性을 나타내어 腫瘍細胞를 殺減하는 同時에 骨髓의 造血機能을 抑制하고 消化器官 및 全身에 反應하여 副作用을 일으키며, 人體의 免疫機能을 低下시키기 때문에 抗癌治療의 副作用을 減少시키면서도 癌細胞에 대해 特異的 作用을 나타내는 治療法의 開發이 要求되고 있어 最近 韓醫學界에서도 港癌劑의 副作用 減少에 대한 韓方療法의 硏究가 활발히 進行됨에 따라 저자도 化學療法劑에 의한 肝과 骨髓의 副作用을 抑制하기 위한 方法의 일환으로 四物湯의 效果에 대하여 硏究 檢討하고자 하였다. 방법 : 正堂白鼠에 化學療法劑의 하나인 cyclophosphamide를 注射하여 副作用을 誘發한 후 四物湯을 投與하고 GOT·GPT活性度와 BUN 및 creatininc 含量을 測定하고, 骨髓 副作用에 미치는 效果를 檢査하기 위하여 白血球數 赤血球數 血小板數를 測定하였으며, 다시 腹腔癌을 誘發시킨 雄性 생쥐에 cyclophosphamide를 多量 投與하여 副作用을 誘發하고 四物湯을 投與하여 혈액검사를 함실시함 결론 : GOT活性度는 有意性 있는 減少를 보였고, GOT活性度는 減少하는 傾向이 있었으나 有意性은 없었으며. BUN 및 creatinine 含量은 正堂群과 큰 差異를 보이지 않았다. 白血球·赤血球·血小板數를 測定한 結果 癌을 誘發하지 않은 白鼠의 경우에는 四物湯投與群에서 對照群에 北하여 各各 有意性있는 增加를 보였으나, 擔癌생쥐에 있어서는 모두 對照群과 별다른 差異가 없었다. 결과 : 四物湯은 cyclophsophamide에 의한 肝臟 賢臟 骨髓에 대한 副作用을 抑制하는 效果가 있을 것으로 推定할 수 있으며, 앞으로의 硏究를 通하여 癌治療에 應用될 수 있을 것으로 期待된다. In order to investigate the effects of Siwutang on reducing side-effects of anti-cancer agent to the rats, and mice which have abdominal tumor after Sarcoma-180 cells were transplanted, cyclophosphamide, the anti-cancer agent, was injected in onder to induce the side-effects, and the extract of siwutang was administrated through mouth from 5 days bfore the injection, and observe GOT, GPT, BUN, creatinine, number of leukocyte, erythrocyte and thrombocyte. The results are summarized as follows : 1. In the effects of the inhibition of the side-effect on the liver function, the GOT activity was proved to be reduced by the Siwutang, but GPT activity was not changed. 2. In the effect of the inhibitory effect on the side-effect on the kidney function, the BUN and creatinine remained unchanged in all groups. 3. In the number of leukocyte, erythrocyte and thrombocyte, in the study on the no cancer-carrier rats, sample group showed increase compared with the control group. While, there was no significant increase in the study on the cancer-carrier mice. As the result of above experiment, Siwutang has proven to have inhibitory effect on side-effects of anti-cancer agents. We can conclude that Siwutang will be useful for cure of the cancer.

남자대학생들의 운동 형태에 따른 혈중 GOT?GPT 농도의 변화

허만동 한국체육과학회 2007 한국체육과학회지 Vol.16 No.1

The purpose of this study was compare the change of body composition, physical fitness, GOT and GPT according to different 12 weeks to exercise type for aerobic exercise and resistance training in college man. The subjects consisted of groups of aerobic exercise group(n=10) and resistance training group(n=10). The aerobic exercise program was RPE 13-14(40min/day) and resistance exercise was 8-12 RM 3-set. Statistical technique for data analysis were paired t-test(performed to verify the difference between before and after the exercise), independent t-test(performed to verify the difference 2 groups) and 2 way repeated ANOVA to detect different two groups at each point.The experimental results from this study are following. As results of the aerobic and resistance exercise group were significantly decreased body fat weight and % body fat. lean body mass were significantly increased only resistance exercise group. WHR were significantly increased to two groups, only aerobic exercise group. The change of muscle strength and trunk flexion were significantly increased to two groups, grip strength and trunk extension were significantly increased only resistance exercise group.agility were significantly increased to two groups, resting heart rate were significantly decreased to two groups. GOT and GPT were no significantly increased to two groups