Growth and Migration of BALB/3T3 Fibroblast Cells on Nano-engineered Silica Beads Surface

Jihee Kim,Prakash Chandra,Jiyoon Yang,Seog Woo Rhee 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.12

In this study, the behavior of cells on the modified surface, and the correlation between the modified substrates and the response of cells is described. A close-packed layer of nano-sized silica beads was prepared on a coverslip, and the adhesion, proliferation, and migration of BALB/3T3 fibroblast cells on the silica layer was monitered. The 550 nm silica beads were synthesized by the hydrolysis and condensation reaction of tetraethylorthosilicate in basic solution. The amine groups were introduced onto the surfaces of silica particles by treatment with 3-aminopropyltrimethoxysilane. The close-packed layer of silica beads on the coverslip was obtained by the reaction of the amine-functionalized silica beads and the (3-triethoxysilyl)propylsuccinic anhydride treated coverslip. BALB/3T3 fibroblast cells were loaded on bare glass, APTMS coated glass, and silica bead coated glass with the same initial cell density, and the migration and proliferation of cells on the substrates was investigated. The cells were fixed and stained with antibodies in order to analyze the changes in the actin filaments and nuclei after culture on the different surfaces. The motility of cells on the silica bead coated glass was greater than that of the cells cultured on the control substrate. The growth rate of cells on the silica bead coated glass was slower than that of the control. Because the close-packed layer of silica beads gave an embossed surface, the adhesion of cells was very weak compared to the smooth surfaces. These results indicate that the adhesion of cells on the substrates is very important, and the actin filaments might play key roles in the migration and proliferation of cells. The nuclei of the cells were shrunk on the weakly adhered surfaces, and the S1 stage in which DNA is duplicated in the cell dividing processes might be retarded. As a result, the rate of proliferation of cells was decreased compared to the smooth surface of the control. In conclusion, the results described here are very important in the understanding of the interaction between implanted materials and biosystems.

Growth and Migration of BALB/3T3 Fibroblast Cells on Nano-engineered Silica Beads Surface

Kim, Jihee,Chandra, Prakash,Yang, Jiyoon,Rhee, Seog Woo Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.12

In this study, the behavior of cells on the modified surface, and the correlation between the modified substrates and the response of cells is described. A close-packed layer of nano-sized silica beads was prepared on a coverslip, and the adhesion, proliferation, and migration of BALB/3T3 fibroblast cells on the silica layer was monitered. The 550 nm silica beads were synthesized by the hydrolysis and condensation reaction of tetraethylorthosilicate in basic solution. The amine groups were introduced onto the surfaces of silica particles by treatment with 3-aminopropyltrimethoxysilane. The close-packed layer of silica beads on the coverslip was obtained by the reaction of the amine-functionalized silica beads and the (3-triethoxysilyl)propylsuccinic anhydride treated coverslip. BALB/3T3 fibroblast cells were loaded on bare glass, APTMS coated glass, and silica bead coated glass with the same initial cell density, and the migration and proliferation of cells on the substrates was investigated. The cells were fixed and stained with antibodies in order to analyze the changes in the actin filaments and nuclei after culture on the different surfaces. The motility of cells on the silica bead coated glass was greater than that of the cells cultured on the control substrate. The growth rate of cells on the silica bead coated glass was slower than that of the control. Because the close-packed layer of silica beads gave an embossed surface, the adhesion of cells was very weak compared to the smooth surfaces. These results indicate that the adhesion of cells on the substrates is very important, and the actin filaments might play key roles in the migration and proliferation of cells. The nuclei of the cells were shrunk on the weakly adhered surfaces, and the S1 stage in which DNA is duplicated in the cell dividing processes might be retarded. As a result, the rate of proliferation of cells was decreased compared to the smooth surface of the control. In conclusion, the results described here are very important in the understanding of the interaction between implanted materials and biosystems.

Development of a Novel Cell Surface Attachment System to Display Multi-Protein Complex Using the Cohesin-Dockerin Binding Pair

( Hyeok-jin Ko ),( Heesang Song ),( In-geol Choi ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.8

Autodisplay of a multimeric protein complex on a cell surface is limited by intrinsic factors such as the types and orientations of anchor modules. Moreover, improper folding of proteins to be displayed often hinders functional cell surface display. While overcoming these drawbacks, we ultimately extended the applicability of the autodisplay platform to the display of a protein complex. We designed and constructed a cell surface attachment (CSA) system that uses a non-covalent protein-protein interaction. We employed the high-affinity interaction mediated by an orthogonal cohesin-dockerin (Coh-Doc) pair from Archaeoglobus fulgidus to build the CSA system. Then, we validated the orthogonal Coh-Doc binding by attaching a monomeric red fluorescent protein to the cell surface. In addition, we evaluated the functional anchoring of proteins fused with the Doc module to the autodisplayed Coh module on the surface of Escherichia coli. The designed CSA system was applied to create a functional attachment of dimeric α-neoagarobiose hydrolase to the surface of E. coli cells.

플라즈마 표면 처리 된 PDMS 막의 인장으로 생성된 미세패턴을 이용한 세포 접촉 유도 연구

유사무엘(Samuel Yu),최성균(Seongkyun Choi),요시츠미 마스모토(Yoshizumi Masumoto),타이치 히로세(Taichi Hirose),마사히토 반(Masahito Ban),김선민(Sun Min Kim) 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.10

Recently, contact guidance of cells using micro/nanopatterns has been widely investigated for mimicking in vivo environments. In this study, we used different types of micro-patterned surface as contact guidance for enhancing cell adhesion and growth. Micro/nanopatterns were created by stretching PDMS membrane exposed to plasma. Three different types of cells were cultured for 3 days on patterned PDMS surface to analyze cell growth and proliferation with phase contrast microscope. Also, the distribution of angle between patterns axis and cells axis were measured. Cultured cells on patterned PDMS surface were elongated along contact guidance and morphology of them was changed according to size of pattern and type of cell. This study will be helpful for the study on cell-surface interactions.

Quantitative Analysis of Growth of Cells on Physicochemically Modified Surfaces

Prakash Chandra,김지혜,Seog Woo Rhee 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.2

In this study, we describe the most expected behavior of cells on the modified surface and the correlation between the modified substrates and the response of cells. The physicochemical characteristics of substrates played an essential role in the adhesion and proliferation of cells. Glass and polymer substrates were modified using air plasma oxidation, and the surfaces were coated with self-assembled monolayer molecules of silanes. The PDMS substrates embedded with parallel micropatterns were used for evaluation of the effect of topologically modified substrate on cellular behaviour. BALB/3T3 fibroblast cells were cultured on different surfaces with distinct wettability and topology, and the growth rates and morphological change of cells were analyzed. Finally, we found the optimum conditions for the adhesion and proliferation of cells on the modified surface. This study will provide insight into the cell-surface interaction and contribute to tissue engineering applications.

Quantitative Analysis of Growth of Cells on Physicochemically Modified Surfaces

Chandra, Prakash,Kim, Jihee,Rhee, Seog Woo Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.2

In this study, we describe the most expected behavior of cells on the modified surface and the correlation between the modified substrates and the response of cells. The physicochemical characteristics of substrates played an essential role in the adhesion and proliferation of cells. Glass and polymer substrates were modified using air plasma oxidation, and the surfaces were coated with self-assembled monolayer molecules of silanes. The PDMS substrates embedded with parallel micropatterns were used for evaluation of the effect of topologically modified substrate on cellular behaviour. BALB/3T3 fibroblast cells were cultured on different surfaces with distinct wettability and topology, and the growth rates and morphological change of cells were analyzed. Finally, we found the optimum conditions for the adhesion and proliferation of cells on the modified surface. This study will provide insight into the cell-surface interaction and contribute to tissue engineering applications.

Replication of nano Petri dish for studying cell-nanotopography interaction by injection molding process

Kyoung Je Cha,Dong Sung Kim 한국생산제조학회 2012 한국생산제조시스템학회 학술발표대회 논문집 Vol.2012 No.10

Surface Plasmon Resonance Study of SKBR3 Antibody and Antigen Interaction by Using Spectroscopic Ellipsometry

전동렬,김윤복,우민아,Myunghaing Cho 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.3

Using spectroscopic ellipsometry as a surface plasmon resonance (SPR) detector, we studied the antibody-antigen interaction of the Her-2/neu protein from SKBR3 breast cancer cells. Chips were prepared by immobilizing the HER2 antibody on a linker-coated Au surface and the SPR was measured after dipping the chip in SKBR3 buffer and drying. The shifts of the SPR peak at each step of the chip preparation and the corresponding morphology change in the atomic force microscopy images suggested that this method could be useful for the diagnosis of cancerous cells in vitro.

임플란트-조직 계면에서의 부착단백질의 역할

박지선 ( Ji Sun Park ),이재봉 ( Jae Bong Lee ),김진 ( Jin Kim ),임영준 ( Young Jun Lim ) 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.4

Upon fixture implantation, the first interaction between the fixture and the host organism begins at the surface of the fixture as it encounters the blood and the interstitial fluid of the host. Especially, molecules including proteins, derived from the body fluid, adsorbs to the surface of the fixture, and the process of it acts as a signal forwarding following biological reactions, rendering various cells to go through adhesion and reorganization, which enables osseointegration of the fixture within the bone structure. In this review, adhesion proteins that are known to adsorb to the implant surface in initial stage are focused. First, the general process, mediated through adhesion proteins such as integrins in the micro-environment consisting of the surface and cells, are reviewed. Next, the studies of adhesion proteins in vitro and in vivo are discussed. Lastly, this paper will describe how these proteins can affect cell spreading, proliferation and differentiation in the implant- tissue interface. An understanding of the special features of the adhesion proteins and their receptors is a prerequisite for the development of novel implant surfaces.

Non-Thermal Atmospheric-Pressure Plasma Possible Application in Wound Healing

Haertel, Beate,von Woedtke, Thomas,Weltmann, Klaus-Dieter,Lindequist, Ulrike The Korean Society of Applied Pharmacology 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.6

Non-thermal atmospheric-pressure plasma, also named cold plasma, is defined as a partly ionized gas. Therefore, it cannot be equated with plasma from blood; it is not biological in nature. Non-thermal atmospheric-pressure plasma is a new innovative approach in medicine not only for the treatment of wounds, but with a wide-range of other applications, as e.g. topical treatment of other skin diseases with microbial involvement or treatment of cancer diseases. This review emphasizes plasma effects on wound healing. Non-thermal atmospheric-pressure plasma can support wound healing by its antiseptic effects, by stimulation of proliferation and migration of wound relating skin cells, by activation or inhibition of integrin receptors on the cell surface or by its pro-angiogenic effect. We summarize the effects of plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of plasma. The outcome of first clinical trials regarding wound healing is pointed out.