Biosorption of Tm(III) by free and polysulfone-immobilized Turbinaria conoides biomass

S. Rangabhashiyam,K. Vijayaraghavan 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-

The current investigation focus on the use of brown alga Turbinaria conoides (TC) and polysulfoneimmobilized Turbinaria conoides (PITC) for the biosorption of rare earth element Thulium (Tm(III)) fromaqueous systems. To attain the highest removal of Tm(III), the process parameters including theequilibrium pH, Tm(III) concentration and biosorption time were optimized. The characterizationtechniques were used to analyse the morphological features and elemental analysis of the biosorbents. The isotherm models of selective two and three parameters were subjected to inspect the equilibriumdata. The biosorption datafitted to the different biosorption kinetic models. Results illustrated that bothTC and PITC displayed higher biosorption capacity of 200.5 and 157.9 mg/g, respectively. Through kinetictrials, we identified delay in equilibrium attainment for PITC and the data were described withintraparticle diffusion model. TC and PITC presented comparable results with biosorption equilibriumattained in 200 min, optimum equilibrium solution pH 5.0 and exhibited maximum biosorption capacityfor the initial Tm(III) concentrations of 500 mg/L. Recovery studies represented that the maximumdesorption efficiency attained using 0.01 M HCl and PITC presented potential uptake capacity of Tm(III)during the entire 10 biosorption–desorption cycles.

Biosorption Behaviors of Biosorbents Based on Microorganisms Immobilized by Ca-alginate for Removing Lead (II) from Aqueous Solution

Xiaoli Li,Yaolong Wang,Yanfeng Li,Lincheng Zhou,Xiaoning Jia 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.4

Multiple microorganisms directly or treated with NaOH were immobilized by using Ca-alginate embedding to form biosorbents I and II, successively. The biosorption behaviors of biosorbents I and II for Pb(II)from aqueous solution were investigated in a batch system. Effects of solution pH, initial metal concentration, biosorbent dosage, contact time, temperature, and ionic strength on the adsorption process were considered to study the biosorption equilibrium, kinetics, thermodynamics, and mechanism of Pb(II) ion adsorption on the 2 types of biosorbents. The results showed that the adsorption capacity of biosorbent II for Pb(II) was higher than that of biosorbent I, and biosorbent II had a faster adsorption rate for Pb(II) ions. According to FTIR spectra, the carboxyl,amine, and hydroxyl groups on the biomass surface were involved in the biosorption of Pb(II). EDX analysis showed that ion exchange may be involved in the biosorption process, and the morphology observed by SEM micrograph of biosorbent I was completely different from that of biosorbent II. Desorption and regeneration experiments showed that the 2 types of biosorbents could be reused for 3 biosorption-desorption cycles without significant loss of their initial biosorption capacities.

Biosorption에 의한 유기물 흡착특성에 관한 연구

김동하 ( Kim Dong Ha ),김경진 ( Kim Gyeong Jin ),유환모 ( Yu Hwan Mo ),박선영 ( Park Seon Yeong ) 한국물환경학회 2003 한국물환경학회지 Vol.19 No.4

In this research, we investigated the possibility of applying biosorption reaction to the advanced wastewater system with the analysis of the various operating conditions using the contact stabilization system. Biosorption is common reaction that makes biological removal, adsorption and coagulation achieved using the MLSS in the endogenous respiration. In the paper, we researched the influence of operating conditions, such as MLSS. adsorption time, DO, and activity of microorganism etc. in the biosorption reaction. And it was calculated the mass-balance lor the quantity analysis. The results were abtained as follows: 1. The optimal biosorption condition were determined adsorption time 15min, MLSS 2200㎎/L. DO 0.2㎎/L. Under this optimal condition, the carbon adsorption ratio was 65.5% and the total removal ratio was 81%. 2. The T-N. T-P adsorption ratio is 25.3%, 50.8% respectively under the optimal biosorption condition 3. The adsorption velocity is expressed as the Freundlich isotherm : COD_(ad)/MLSS=19.44(COD_(ex))^(0537). 4. In the optimal biosorption condition, the BOD_(5) amounts are 12.5g/day, which are able to remove the 3.7gNO₃-N/day.

Metal Biosorption by Surface-Layer Proteins from Bacillus Species

Mariana Claudia Allievi,Sabbione Florencia,Prado Acosta Mariano,Palomino Maria Mercedes,Ruzal Sandra M.,Sanchez Rivas Carmen 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.2

Bacillus species have been involved in metal association as biosorbents, but there is not a clear understanding of this chelating property. In order to evaluate this metal chelating capacity, cultures and spores from Gram-positive bacteria of species either able or unable to produce surface layer proteins (S-layers) were analyzed for their capacity of copper biosorption. Only those endowed of S-layers, like Bacillus sphaericus and B. thuringiensis, showed a significant biosorption capacity. This capacity (nearly 50%) was retained after heating of cultures, thus supporting that structural elements of the envelopes are responsible for such activity. Purified Slayers from two Bacillus sphaericus strains had the ability to biosorb copper. Copper biosorption parameters were determined for strain B. sphaericus 2362, and after analyses by means of the Langmuir model, the affinity and capacity were shown to be comparable to other bacterial biosorbents. A competitive effect of Ca2+ and Zn2+, but not of Cd2+, was also observed, thus indicating that other cations may be biosorbed by this protein. Spores that have been shown to be proficient for copper biosorption were further analyzed for the presence of Slayer content. The retention of S-layers by these spores was clearly observed, and after extensive treatment to eliminate the S-layers, the biosorption capacity of these spores was significantly reduced. For the first time, a direct correlation between S-layer protein content and metal biosorption capacity is shown. This capacity is linked to the retention of S-layer proteins attached to Bacillus spores and cells.

The Past, Present, and Future Trends of Biosorption

Park, Dong-Hee,Yun, Yeoung-Sang,Park, Jong-Moon 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.1

The discovery and further development of biosorption phenomena provide a basis for a whole new technology aimed at the removal of various pollutants or the recovery of valuable resources from aqueous systems. Today, biosorption is one of the main components of environmental and bioresource technology. Since the status of scientific development of a technology can be reflected through analyses of the literatures pertaining to it, in this review, we qualitatively examine almost all aspects of biosorption research. A range of subjects are covered, including the initial history, raw materials, mechanisms, instrumental tools, process factors, modification and immobilization methods, recovery and regeneration, continuous processes, commercial application, and modeling studies of biosorption. Finally, we summarized the important considerations of the current research on biosorption, as well as the suggestions for its future directions. We believe that this review will prove to be useful for scientists and engineers in the performance of their research into biosorption.

Multiple biosorption–desorption cycles in a fixed-bed column for Pb(II) removal by acid-treated olive stone

M.A. Martın-Lara,G. Blazquez,A. Ronda,I.L. Rodrıguez,M. Calero 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.3

Biosorption of Pb2+ from aqueous solutions and desorption of Pb2+ from acid-treated olive stone were studied. Equilibrium screening tests of lead desorption established a solution of 0.3 M hydrochloric acid as the most appropriate eluting agent. Fixed-bed biosorption and desorption recovery curves for Pb2+were obtained for multiple consecutive biosorption–desorption cycles and the biosorption properties of the biomass for Pb2+ were compared after each cycle to evaluate the stability of the biomass. The biosorbent retained most of its original uptake capacity over fourteen cycles of use. Life factor calculation revealed that biosorbent bed would be completely exhausted after 71.3 cycles.

Removal, Recovery, and Process Development of Heavy Metal by Immobilized Biomass Methods

Ahn, Kab Hwan,Shin, Yong Kook,Suh, Kuen Hack 한국환경과학회 1997 한국환경과학회지 Vol.6 No.1

Heavy metal adsorption by microbial cells is an alternative to conventional methods of heavy metal removal and recovery from metal-bearing wastewater. The waste Saccharomyces cerevisiae is an inexpensive, relatively available source of biomass for heavy metal biosorption. Biosorption was investigated by free and immobilized-S. cerevisiae. The order of biosorption capacity was Pb>Cu>Cd with batch system. The biosorption parameters had been determined for Pb with free cells according to the Freundlich and Langmuir model. It was found that the data fitted reasonably well to the Freundlich model. The selective uptake of immobilized-S. cerevisiae was observed when all the metal ions were dissolved in a mixed metals solution(Pb, Cu, Cr and Cd). The biosorption of mixed metals solution by immobilized-cell was studied in packed bed reactor. The Pb uptake was investigated in particular, as it represents one of the most widely distributed heavy metals in water. We also tested the desorption of Pb from immobilized-cell by using HCl, H_2SO_4 and EDTA.

Biosorption of cadmium by Enterobacter ludwigii isolated from soil contaminated with cadmium near a coal-fired power plant in Korea

Tran Thi Minh,Lee Jong-Un 한국자원공학회 2023 Geosystem engineering Vol.26 No.6

This study aimed to isolate and characterize indigenous bacteria from cadmium-contaminated soil around a coal-fired power plant in Korea for their potential use in biosorption. The 16S ribosomal RNA analysis identified Enterobacter ludwigii G17–1 in the soil, exhibiting a remarkably high minimum inhibitory concentration (2,500 mg/L) for cadmium. The efficiency of cadmium biosorption was investigated under different pH levels (6–9), temperatures (15–40°C), and initial cadmium concentrations (25–100 mg/L), using both live and dead G17–1. The live G17–1 strain exhibited a maximum biosorption efficiency of 50% for 25 mg/L cadmium at 24 hours, while the highest efficiency achieved with dead G17–1 was 48% at 1 hour. The biosorption capacity decreased as the initial cadmium concentration increased. These findings suggest that the isolated bacterium, E. ludwigii G17–1, holds potential for the bioremediation of cadmium-contaminated water and wastewater.

혐기-간헐포기 공정에서 기질의 종류에 따른 Biosorption 특성 평가

신응배 ( Sin Eung Bae ),김미경 ( Kim Mi Gyeong ),김재헌 ( Kim Jae Heon ) 한국물환경학회 2003 한국물환경학회지 Vol.19 No.5

In this study, five representative substrates (glucose, acetate, starch, humic acid, and glycogen) having different characteristics of biodegradability and microbial acclimation were chosen for evaluating biosorption capacity using activated sludge and autoclaved sludge as sorbents, and also effects of contact time, substrate concentration and anaerobic/aerobic conditions were investigated. Results showed that the anaerobic biosorption finished within 10 minutes after contact with substrates and the removal of glucose was two times greater than that of acetate. Considering that the microbes used in this study have been acclimated to glucose, this implies that the microbial acclimation is an important factor for biosorption. In cases of glucose, acetate, and starch, the substrate was removed by biological mechanism rather than by physicochemical mechanisms. The sorption experiments using the autoclaved sludge showed the constant substrate removal of 10-20 ㎎ COD/g SS. Results also showed that the biosorption of the activated sludge fitted the Langmuir isotherm and was largely affected by substrate affinity, biodegradability and microbial acclimation.

Recent Trends in the Biosorption of Heavy Metals: A Review

Sag, Yesim,Kutsal, Tulin The Korean Society for Biotechnology and Bioengine 2001 Biotechnology and Bioprocess Engineering Vol.6 No.6

Considerable attention has been focused in recent years upon the field of biosorption for the removal of metal ions from aqeous effluents. Compared to other technologies, the advan-tages of biosortption are the high purity of the treated waste water and the cheap raw material. Really, the first major challenge for the biosorption field is to select the most promising types of biomass. Abundant biomass types either generated as a waste by-product of large-scale industrial fermentations particularly fungi or certain metal-binding seaweeds have gained importance in re-cent years due to their natural occurrence, low cost and, of course good performance in metal biosorption. Industrial solutions commonly contain multimetal systems or several organic and in organic substances that form complexes with metals at relatively high stability forming a very complex environment. When several components are present, interference and competition phe-nomena for sorption sites occur and lead to a more complex mathematical formulation of the process. The most optimal configuration for continuous flow-biosorption seems to the packed-bed column which gets gradually from the feed to the solution exit end. Owing to the com-petitive ion exchange taking place in the column, one or more of the metals present even at trace levels may overshot the acceptable limit in the column effluent before the breakthrough point of the trargeted metal. Occurrence of 'overshoot's and impact on havey metal removal has not been analyzed enough. New trends in biosorption are discussed in this review.