HC001의 아토피성 피부염에 대한 항염증 효능 및 기전 연구

최유연,김미혜,금창준,최영진,황만기,손영주,정혁상,양웅모,Choi, You-Youn,Kim, Mi-Hye,Kum, Chang-Jun,Choi, Young-Jin,Hwang, Man-Ki,Sohn, Young-Joo,Jung, Hyuk-Sang,Yang, Woong-Mo 대한한의학방제학회 2012 大韓韓醫學方劑學會誌 Vol.20 No.1

본 실험에서는 피부질환을 위한 새로 구성된 HC001의 처방이 외용제로서 아토피성 피부질환에서의 항염증 효능을 확인하였다. H&E염색을 통한 조직학적 검사에서 DNCB로 유도된 진피 및 표피의 과각화증이 HC001 국소도포로 인해 호전되는 것을 관찰되었으며, 염증과정에서 중요한 지표인 NF-${\kappa}B$의 활성이 억제됨을 확인하였다. 이를 통해 HC001이 아토피성 피부질환의 외용제 사용에 있어 일정한 효과를 나타낼 수 있을 것으로 기대 된다. Objectives : The purpose of this study is to examine the effects of external application of HC001 on atopic dermatitis. HC001, newly composed herbal medicine combinations contains 9 medicinal herbs which are known to have anti-inflammatory and anti-allergic effects. We investigated the anti-inflammatory effects of HC001 on atopic dermatitis-like skin lesions in mice. Methods : Seven-week-old BALB/c mice were sensitized with DNCB to develop atopic dermatitis. Animals were divide into three group: Normal, DNCB (Negative control group), HC001 (Experimental group, treated with DNCB and HC001). Skin sections were stained with H&E to measure the thicknesses of the epidermis and dermis, respectively. The expression of NF-${\kappa}B$ protein was measured by western blotting analysis in skin lesion. Results : Topical HC001 treatment significantly restored the skin thickening and hyperplasia of the epidermis and dermis compared with DNCB-sensitized group in histopathological analyse. In addition, HC001 inhibited the expression of NF-${\kappa}B$ protein increased in DNCB-induced atopic dermatitis-like skin lesions in mice. Conclusions : These results suggest that HC001 may be useful as an external application agent for atopic dermatitis based on reductions of various inflammatory responses.

이동 Ad-Hoc 네트워크 환경에 적합한 스트림 암호 HC-128의 부채널 안전성 분석

배기석(KiSeok Bae),박영호(YoungHo Park),문상재(SangJae Moon) 한국산업정보학회 2012 한국산업정보학회논문지 Vol.17 No.6

최근 eSTREAM 공모사업에서 소프트웨어 분야로 최종 선정된 HC-128 알고리듬은 제한된 메모리 환경에서 고속 암호화가 가능하여 이동 ad-hoc 네트워크에 적합한 스트림 암호이다. 본 논문은 실제 구현되었을 때 발생할 수 있는 부채널 분석 공격에 대한 스트림 암호 HC-128 알고리듬의 안전성을 분석한다. 먼저 부채널 분석 공격에 대한 안전도가 낮은 것으로 판정하였던 기존 분석 방법의 누락된 부분을 밝히고, 올바른 분석 과정에서 필요한 계산 복잡도를 계산하여 HC-128 알고리듬의 부채널 분석 공격에 대한 안전성을 재평가하였다. 그 결과, 비밀키를 알아내기 위해서는 타 스트림 암호에 비해 훨씬 큰 약 2<SUP>64</SUP>만큼의 복잡도가 필요하므로 스트림 암호 HC-128는 부채널 분석 공격에 안전한 것으로 평가된다. The HC-128 stram cipher which selected for the final eSTREAM portfolio is suitable for mobile Ad-Hoc network environments because of the ability of high-speed encryption in restricted memory space. In this paper, we analyzed the vulnerability of side channel analysis attack on HC-128 stream cipher. At the first, we explain a flaw of previous theoretical analysis result which defined the complexity of side-channel attack of HC-128 stream cipher as "low" and then re-evaluate the security against side-channel attack by estimating the concrete complexity for recovering the secret key. As a result, HC-128 stream cipher is relatively secure against side-channel attack since recovering the secret key have 2<SUP>64</SUP> computation complexity which is higher than other stream cipher"s one.

Cloning and characterization of a selenium-independent glutathione peroxidase (HC29) from adult Haemonchus contortus

Wei Sun,Xiaokai Song,Ruofeng Yan,Lixin Xu,Xiangrui Li 대한수의학회 2012 JOURNAL OF VETERINARY SCIENCE Vol.13 No.1

The complete coding sequence of Haemonchus (H.) contortus HC29 cDNA was generated by rapid amplification of cDNA ends in combination with PCR using primers targeting the 5´- and 3´-ends of the partial mRNA sequence. The cloned HC29 cDNA was shown to be 1,113 bp in size with an open reading frame of 507 bp, encoding a protein of 168 amino acid with a calculated molecular mass of 18.9 kDa. Amino acid sequence analysis revealed that the cloned HC29 cDNA contained the conserved catalytic triad and dimer interface of selenium-independent glutathione peroxidase (GPX). Alignment of the predicted amino acid sequences demonstrated that the protein shared 44.7∼80.4% similarity with GPX homologues in the thioredoxin-like family. Phylogenetic analysis revealed close evolutionary proximity of the GPX sequence to the counterpart sequences. These results suggest that HC29 cDNA is a GPX, a member of the thioredoxin-like family. Alignment of the nucleic acid and amino acid sequences of HC29 with those of the reported selenium-independent GPX of H. contortus showed that HC29 contained different types of spliced leader sequences as well as dimer interface sites, although the active sites of both were identical. Enzymatic analysis of recombinant prokaryotic HC29 protein showed activity for the hydrolysis of H2O2. These findings indicate that HC29 is a selenium-independent GPX of H. contortus.

Estrogen Receptor Enhances the Antiproliferative Effects of Trichostatin A and HC-toxin in Human Breast Cancer Cells

Kyung Nan Min,Min Jung Cho,김대기,신윤용 대한약학회 2004 Archives of Pharmacal Research Vol.27 No.5

Trichostatin A, an antifungal antibiotics, and HC-toxin are potent and specific inhibitors of histone deacetylase activity. Histone deacetylase inhibitors are new class of chemotherapeutic drugs able to induce tumor cell apoptosis and/ or cell cycle arrest. In this study, the antiproliferative activities of trichostatin A and HC-toxin were compared between estrogen receptor positive human breast cancer cell MCF-7 and estrogen receptor negative human breast cancer cell MDA-MB-468. Trichostatin A and HC-toxin showed potent antiproliferative activity in both MCF-7 and MDA-MB-468 cells. In MCF-7 cells that contain high level estrogen receptor, trichostatin A and HC-toxin brought about three-times more potent cell growth inhibitory effect than estrogen receptor negative MDA-MB-468 cells. Both trichostatin A and HC-toxin showed cell cycle arrest at G2/M phases of MCF-7 and MDA-MB-468 cells in a dose- and time- dependent manner. Trichostatin A and HC-toxin also induced apoptosis from MCF-7 and MDA-MB- 468 cells in a dose- and time-dependent manner. Results of this study suggested that antiproliferative effects of trichostatin A and HC-toxin might be involved in estrogen receptor signaling pathway, but cell cycle arrest and apoptosis of trichostatin A and HC-toxin might not be involved in estrogen receptor system of human breast cancer cells.

Estrogen Receptor Enhances the Antiproliferative Effects of Trichostatin A and HC-toxin in Human Breast Cancer Cells

Min, Kyung-Nan,Cho, Min-Jung,Kim, Dae-Kee,Sheen, Yhun-Yhong The Pharmaceutical Society of Korea 2004 Archives of Pharmacal Research Vol.27 No.5

Trichostatin A, an antifungal antibiotics, and HC-toxin are potent and specific inhibitors of histone deacetylase activity. Histone deacetylase inhibitors are new class of chemotherapeutic drugs able to induce tumor cell apoptosis and/or cell cycle arrest. In this study, the antiproliferative activities of trichostatin A and HC-toxin were compared between estrogen receptor positive human breast cancer cell MCF-7 and estrogen receptor negative human breast cancer cell MDA-MB-468. Trichostatin A and HC-toxin showed potent antiproliferative activity in both MCF-7 and MDA-MB-468 cells. In MCF-7 cells that contain high level estrogen receptor, trichostatin A and HC-toxin brought about three-times more potent cell growth inhibitory effect than estrogen receptor negative MDA-MB-468 cells. Both trichostatin A and HC-toxin showed cell cycle arrest at G$_2$/M phases of MCF-7 and MDA-MB-468 cells in a dose- and time- depen- dent manner. Trichostatin A and HC-toxin also induced apoptosis from MCF-7 and MDA-MB-468 cells in a dose- and time-dependent manner. Results of this study suggested that antipro-liferative effects of trichostatin A and HC-toxin might be involved in estrogen receptor signaling pathway, but cell cycle arrest and apoptosis of trichostatin A and HC-toxin might not be involved in estrogen receptor system of human breast cancer cells.

자궁경부의 인유두종 바이러스 검출에 있어 HPV DNA chip 검사의 임상적 유용성

이제훈 ( Je Hoon Lee ),이기헌 ( Ki Heon Lee ),이인호 ( In Ho Lee ),우혁준 ( Hyuk Jun Woo ),성석주 ( Seok Ju Seong ),김태진 ( Tae Jin Kim ),임경택 ( Kyung Taek Lim ),심재욱 ( Jae Uk Shim ),박종택 ( Chong Taik Park ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.10

목적: 비정상 자궁경부 세포검사 소견을 보인 환자 중 질확대경 검사나 원추절제술을 통해 조직학적 병변의 유무가 확인된 환자들을 대상으로 HC-II와 HPV DNA chip 검사를 시행하여 각 검사의 임상적 유용성을 확인하고자 한다. 연구 방법: 2004년 7월부터 10월까지 비정상 자궁경부 세포검사 결과로 본원 부인종양센터에서 질확대경하 조직검사 또는 원추절제술을 시행하여 조직학적 소견이 확인된 200명의 환자에서 HPV 감염을 확인하기 위해 HC-II와 HPV DNA chip 검사를 동시에 시행하여 HPV 검출에 두 검사방법의 일치율을 비교하고, HPV DNA chip의 결과로 확인된 HPV 아형들을 분석하였다. 결과: 200명의 대상 환자 중 HC-II와 HPV DNA chip의 결과가 일치한 경우는 171명 (85.5%) 이었다. 비정상 자궁경부세포검사를 보인 환자 중 조직 검사 상 편평콘딜로마 이상의 병변이 확인된 111명의 환자에서, HC-II와 HPV DNA chip 검사의 민감도는 각각 91.0%와 88.3%였다. HPV DNA chip 검사에서 검출된 HPV의 아형들은 HPV-16이 가장 흔하게 검출되었고 (14.7%), 그 다음으로 HPV-58과 HPV-18이 14.1%와 9.8%의 순으로 검출되었다. 결론: 자궁경부의 병변에서 HPV의 검출에 사용되는 HPV DAN chip 검사는 HC-II에 견줄만한 민감도를 갖는 효과적인 방법이다. 또한 HPV DNA chip은 감염된 HPV의 아형을 확인하고 중복감염 등 임상적으로 유용한 정보를 제공하는 장점을 가지고 있다. Objective: This study was performed to investigate the efficacy of HPV DNA chip method for detection and genotyping of various human papillomavirus in the patients with intraepithelial lesions of uterine cervix. Methods: The study subjects included two hundred patients with abnormal Pap smear from July 2004 to October 2004. After confirmed the pathological status of the cervix with colposcopic biopsy or conization, we evaluated for HPV infection and genotyping with the commercially available Hybrid-Capture II assay (HC-II) and HPV DNA chip. Then we compared the concordance rate between the two methods for the detection of HPV and analysed the HPV genotypes. Results: We compared the results in HPV DNA chip with those in HC-II. In result, the concordance rate between the two methods for the detection of HPV was 85.5% (171 of 200 cases). In 111 patients confirmed the presence of lesions higher than flat condyloma in cervix by pathologc examination, sensitivities of HC-II and HPV DNA chip in detecting HPV were 91.0% and 88.3%, respectively. In HPV DNA chip, HPV-16 was the most frequent type (14.7%) in all patients, the next frequent types were HPV-58 (14.1%) and HPV-18 (9.2%). Conclusion: We confirmed that HPV DNA chip method was as sensitive and effective method for detecting HPV in cervical lesions as HC-II. And that it would provide useful clinical information on genotyping and multiple infections of HPV.

혈액의 pH와 PCO2, HCO3, dissolved CO2, total CO2의 비교

김종호 ( Chong Ho Kim ),신용섭 ( Yong Sub Shin ),윤기은 ( Ki Eun Yoon ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3

We detennined the blood pH, partial carbondioxide pressure(PC02), concentration of bicarbonate ion(HC03 ), dissolved CO2 with H2C03(DSC02), and concentration of total carbonedioxide(ctC02)of 214 patients in hospital. The patients with pH 7.35-7.45 were classified as the reference group, with pH less than pH 7.35 were classified as the acidosis group, and with pH higher than pH 7.45 were classified as the alkalosis group. Wecompared the distribution, mean value and variation of PC02, HC03, DSC02, ctC02. between each group. πle disσibution of PC02(SDI6.87) in the acidosis group, and HC03 -(SD, 17.219) and ctC02(SD, 19.335) in alkalosis groap showed the largest value in the three groups. The reference patients group showed the smallest distribution of PC02(SD, 8.436), HC03(SD, 5.392), ctC02.(SD, 5.612) in the groups. The acidosis group showed the highest average value of PC02 and DSC02, but the alkalosis group showed the lowest average value of PC02, DSC02. The average value of HC03 and ctC02 showed the highest value in the alkalosis group, but lowest in the acidosis group. The variation rates of the mean of PC02, HC03, DSC02, ctC02 in the acidosis group and alkalosis group, relative to the means of the reference group, were calculated. The acidosis group showed ar increasing variation of PC02 and DSC02, but decreasing variation of HC03 and ctC02. The alkalosis group showed increasing variation of HC03 and ctC02, but decreasing variation of PC02 and DSC02.

Antiproliferative Effect of Trichostatin A and HC-Toxin in T47D Human Breast Cancer Cells

Joung, Ki-Eun,Kim, Dae-Kee,Sheen, Yhun-Yhong The Pharmaceutical Society of Korea 2004 Archives of Pharmacal Research Vol.27 No.6

Histone deacetylase inhibitors are new class of chemotherapeutic drugs able to induce tumor cell apoptosis and/or cell cycle arrest. Trichostatin A, an antifungal antibiotic, and HC-toxin are potent and specific inhibitors of histone deacetylase activity. In this study, we have examined the antiproliferative activities of trichostatin A and HC-toxin in estrogen receptor positive human breast cancer, T47D cells. Both trichostatin A and HC-toxin showed potent antiprolifer-ative efficacy and cell cycle arrest at $G_2/M$ in T47D human breast cancer cells in a dose-dependent manner. Trichostatin A caused potent apoptosis of T47D human breast cancer cells and trichostatin A-induced apoptosis might be involved in an increase of caspase-3/7 activity. HC-toxin evoked apoptosis of T47D cells and HC-toxin induced apoptosis might not be medi-ated through direct increase in caspase-3/7 activity. We have identified potent activities of anti-proliferation, apoptosis, and cell cycle arrest of trichostatin A and HC-toxin in estrogen receptor positive human breast cancer cell line T47D.

수소 풍부 환원제 변화가 Co-Pt/ZSM5 촉매를 사용하는 탈질 HC-SCR 반응에 미치는 영향

김성수,김대영,오세용,유승준,서영석,김진걸 한국수소및신에너지학회 2012 한국수소 및 신에너지학회논문집 Vol.23 No.3

HC-SCR was conducted over Co-Pt/ZSM5 catalyst coated over 200 cpsi cordierite in the condition of atomspheric pressure and 200℃-500℃. Weight ratio of Co/Pt determined from EDX analysis was 8/2, which was almost equal to the weight ratio at preparation step . XPS showed that nitrates within cobalt precursor and chlorine withn Pt precursor were removed. TEM result demonstrated that crystallite size of cobalt and Pt was under 5nm. Among these tested hydrocarbon reductants, isobutane (i-C4H10) showed the highest de-NOx yield of 80% under the condition of the mole ratio of reductant/NOx =1.0 at 180℃. De-NOx yield from HC-SCR was increased as the carbon number of hydrocarbon reductant was increased. The decrease of bonding energy between C and H of HC reductant played a role to increase of de-NOx yield, which indicated that the dissociation step of C-H bond of hydrocarbon molecule might be the rate determining step of HC-SCR. The increase of oxygen concentration in the feed resulted in the decrease of de-NOx yield but the increase of CO and N2O yield.

질 확대경상 비정상 소견을 보인 환자에서 HPV DNA chip과 Hybrid-Capture II assay의 임상적 유용성 비교

김태정,정찬권,이아원,정은선,최영진,이교영,박종섭,Kim, Tae-Jung,Jung, Chan-Kwon,Lee, Ah-Won,Jung, Eun-Sun,Choi, Young-Jin,Lee, Kyo-Young,Park, Jong-Sup 대한세포병리학회 2008 대한세포병리학회지 Vol.19 No.2

This study was performed to compare the efficacy between a DNA chip method and a Hybrid-Capture II assay (HC-II) for detecting human papillomavirus in patients with intraepithelial lesions of the uterine cervix. From May, 2005, to June, 2006, 192 patients with abnormal colposcopic findings received cervical cytology, HC-II and HPV DNA chip tests, and colposcopic biopsy or conization. We compared the results of HC-II and HPV DNA chip in conjunction with liquid based cervical cytology (LBCC) and confirmed the results of biopsy or conization. The sensitivity of the HPV DNA chip test was higher than HC-II or LBCC. The HPV DNA chip in conjunction with LBCC showed higher sensitivity than any single method and higher sensitivity than HC-II with LBCC. We confirmed that the HPV DNA chip test was more sensitive for detecting HPV in cervical lesions than HC-II, and that it would provide more useful clinical information about HPV type and its multiple infections.